Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,705,653 | 0 | T | G | 46.7% | 72.2 / 32.0 | 60 | P46P (CCA→CCC) | dppB | dipeptide/heme ABC transporter permease |
Reads supporting (aligned to +/- strand): ref base T (23/9); new base G (0/28); total (23/37) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.72e-09 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
AAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACA > NC_000913/3705504‑3705789 | aaGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTg < 2:1139913/151‑1 (MQ=255) aGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTgg < 2:490787/151‑1 (MQ=255) gcgGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGga < 1:1017183/151‑1 (MQ=255) cGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATccc < 1:620518/151‑1 (MQ=255) tCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGtt < 2:1176368/148‑1 (MQ=255) cccAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑cgcc > 2:349486/1‑151 (MQ=255) ccAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑cgccc < 1:976249/151‑1 (MQ=255) aaaCCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑cgcccgc > 2:498309/1‑151 (MQ=255) cGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATg > 1:380156/1‑151 (MQ=255) gCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGatcatc > 2:724885/1‑151 (MQ=255) cGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGatcatc > 2:98414/1‑150 (MQ=255) cTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCg > 2:530298/1‑151 (MQ=255) cTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCg > 1:1155850/1‑151 (MQ=255) cTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCg > 1:308811/1‑151 (MQ=255) ttttCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCgg > 1:1132139/1‑151 (MQ=255) aTTAAAATCCCTACACCCCCATACATAACCCCCCAAATGTAATGGAGATACTGCTCCCACCTCTTTTTATCTAACCCGATTC‑CACCCAGCCCCTGCCCGTCACCCTCGGGGGAGTCCCCACTTC‑CCCCCGCCATGATCATCACCGGATc < 1:125500/149‑1 (MQ=255) ttaaaaTGCCTAGATCCCCATGCAAACCCCCCAAAATGTAAGGGGGATCCTGCTGCCACATGGTTTTATCTAAGCCGGTTC‑CAGCAAGCAGCGGCGCGTGCCGCTCGGGGGAGACCCCCCGTC‑CGCCCGCCATGATCATCACCGGATc < 1:860923/145‑1 (MQ=255) gAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCg > 1:373491/1‑151 (MQ=255) aaTGCCGAGGCCGCCTGGCAAAACGCCCAAAATGTAAGGGATTTAGCGCTCCAACATGGTTTTATAT‑AGCCGGCTTCCAGCCGCCGGCTGGTCGTGGCGCGGGGGGGAGCCCCCACGTC‑CGCCCGCCATGATCATCACCGGATCGCCCggg < 2:771307/151‑1 (MQ=255) cctatacccccATGCATACCCCCCAAAATGAAAGGGAGATCCTCCTCCCACATCGTTTTATCAAACCCGATTC‑CAGCCAGCAGCTGCGCGGGACGCTCTGGGGAGACCCCCCGTC‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATc < 1:887084/141‑1 (MQ=255) cctagtcccccATGCATACCCCCCAAAATTTAAGGGAGATCCTGCTCCCACTTCGTTTTATCAAACCCGAGTT‑CAGCCAGCAGCGGCGCGTGACGCTCGGGGGAGATCCCACTTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGa < 1:920294/139‑1 (MQ=255) ccTAGACCGCCATGTATACCCCCCAAAATAAAAGGGGGATACGGCCGCCACCGTGTTTTATCAGAGCCGATTC‑CACCCCGCCGCTGCGGGTGCCGCTGGGGGGAGA‑CCCCCGTTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATc < 2:79178/150‑1 (MQ=255) acccccATGCAAAACCCCCAAAATGTAGTGGAGATCCTGCTGCCCCATCTGTTTACCAAGCCCGAGTT‑CAGCCAGCCGCGGCGCGGGCCGCTCGGGGGAGCCCCCGCGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCAt < 2:184294/143‑1 (MQ=255) aTCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTgg > 1:1115107/1‑151 (MQ=255) ggCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGAc > 2:465755/2‑151 (MQ=255) cGCCTTGCATACCCCCCAAAATGTAAGGGAAATACTGCTCCCACATCGTTTTATCTAAGCCGAGTT‑CAGCCAGCAGCGGCGGGTGCCGCTCGGGGGAGATCCCCCGTC‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGa < 1:158358/150‑1 (MQ=255) cGCCATGCAAACCCCCCAAAATTTAATGAAGATCCTGCTCCCACATCGTTTTATCTAAGCGGAGTT‑CAGCCAGCGGCGGCGCGTGACGCTCGGGGGAGACCCCTCGTC‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGAc < 1:1003042/151‑1 (MQ=255) cGCCATCCATAACCCCCAAAATGTAGGGGAGATACTGCTCCCCCATGGTTTTATCTAAGCCGATTT‑CGGCCAGCGGGTGCGGGTGCCGCTCGGGGGAGCCCCCCCTTC‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGAc < 1:346322/151‑1 (MQ=255) ataaaaacccccaaaaaTTTAAGGGAGATCCGGCGGCCACATCGGTTTATCTACGCCGAGTT‑CAGCCCCCACCGGCGCGTGCCGCTGGGGGAAGCTCCCACGTC‑GGCCCGCCATGATCATCACCGGATCGCCCGGGATCAtgt < 2:149375/129‑1 (MQ=255) tGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAgg > 2:523401/1‑151 (MQ=255) ataccccccaAAATGTAATGGAGATACTGCTGCCCCGTCGTTTTATCTAAGCCGAGTT‑CAGCCAGCAGCGGCGCGTGCCGCTCGGGGGAGACCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACaaa < 1:779857/140‑1 (MQ=255) aTACCGCCCAAAATGTAAGGGAGATACTGCTGCCACATCGTTTTATCTAAGCCGAGTT‑CAGCCGGCAGCTGCGCGTGCCGCTGGGGGGAGATCCCACTTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCaa < 1:553147/151‑1 (MQ=255) cccccaAAATTTAAGGGAGATCCTGCTGCCACATGGTTTTATCAAAGCCGAGTT‑CGGCCAGCAGCTGCGCGTGCCGCTGGGGGGACACCCCGCGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCaaa < 1:807537/146‑1 (MQ=255) cccccaAAATGAAAGGGATATACTGCCCCCACATCGTTTTATCTAAGCCGAGTC‑CAGCCGGCGGCGGCGCGGGACGCTCGGGGGAGACCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAAtgt < 1:259307/149‑1 (MQ=255) aaaTGTAAGGGAGATTCTGCTGCCCCATCTTTTTATCTAACCCGAGTC‑CGGCCGGAGGGTGGGCGTGCCGCTGGGGGGAGATCCCAGGTC‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTgaga < 2:868494/149‑1 (MQ=255) gaaagggggatcgtgccgcCACATGGGTTTATAAAAGCGGATTC‑CGCCCGGCGGCGGCGGGGGCCGTTGGGGGGAGACCCCCCGTC‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAg < 2:863099/134‑1 (MQ=255) gggagatctgcctcccACGTCGTTTTACCTACCCGGAGTC‑CGCCCACCCGCTGCGCGTCACGCTGGGGGGAGACCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATAc < 2:590021/137‑1 (MQ=255) gagttagcgctgcCACTTCGTTTTATCAAAGCCGAGTT‑CCGCCAGCAGCGGCGGGGGGCGCTGGGGGGAGACCCCACGTG‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGt < 2:1057387/136‑1 (MQ=255) gaTCCTGCGCCCACATCGTTTTATCAAAGCCGAGTT‑CGCCCGGCAGCTGCGGGTGTCGCTGGGGGGAGACCCCTTTTC‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCg < 1:851578/149‑1 (MQ=255) gaTACTGCTGCCACATCGGTTTATCTAACCCGATTT‑CAGCCAGCAGCGGCGCGTGCCGCTCGGGGGAGACCCCCCGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGa < 1:513531/150‑1 (MQ=255) tccgcctcccACGTCGTTTTATCAAAGCCGAGTC‑CAGCCAGCAGCGGCGCGTGCCGCTCGGGGGAGACCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATa < 1:966462/142‑1 (MQ=255) ctgctgCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAAc > 2:942382/1‑151 (MQ=255) gctgcCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACg > 2:366000/1‑150 (MQ=255) ctgcCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTg > 2:785567/1‑151 (MQ=255) caTGGGTTTATCTAAGCGGTGTT‑CACCCGGCGGCTGCGGGTCCCGCTGGGGGGAGA‑CCCCCGTTCCGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATg < 1:980530/151‑1 (MQ=255) cGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACg > 1:190390/1‑151 (MQ=255) ggTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACg > 2:874318/1‑150 (MQ=255) tttATCAAAGCGGTGTT‑CAGCCAGCCGCGGCGCGTGCCGCTCGGGGGAGCCCCCACTTC‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAg < 1:863053/150‑1 (MQ=255) aTCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTccc > 1:673885/1‑151 (MQ=255) aGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCgg > 1:461655/1‑150 (MQ=255) aGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCgg > 2:259118/1‑150 (MQ=255) tt‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCgc > 2:896219/1‑147 (MQ=255) tccagccagcGGCTGGGCGGGCCGCTCGGGGGAGACCCCCCTTC‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGgag < 1:221499/148‑1 (MQ=255) tccagccagcCGCGGGGCGTGCCGCTGGGGGGATCCCCCACGTG‑CCCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGga < 2:959227/147‑1 (MQ=255) cagccagcAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAAt > 2:810365/1‑151 (MQ=255) cacccgccggcTGCGCGTGACGCGCGGGACATCCCCCACTGG‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGga < 2:324173/138‑1 (MQ=255) ccagcCGCGGGGCGTGCCGCTGGGGGGAGCCCCCCCTTC‑GGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATa < 2:866491/149‑1 (MQ=255) cagcggCTGCGCGTCACGCTGTGGGGACACCCAAGGTC‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAAt < 2:1167721/147‑1 (MQ=255) gcggGTGCCGCTGTGGGGAGCCCCCCCGTC‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAAc < 1:610333/149‑1 (MQ=255) gggTGCCGCTGGGGGGAGCCCCCCCCTG‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACa < 2:997221/146‑1 (MQ=255) | AAGCGCGGCACGAACTCTTCCCAAACCGGGATGCGGCTTTTCATTGAAATGCCTAGATCGCCATGCATAACGCCCCAAATGTAATGGAGATACTGCTGCCACATCGGTTTATCTAAGCCGAGTT‑CAGCCAGCAGCTGCGCGTGACGCTCTGGGGAGATCCCACGTT‑CGCCCGCCATGATCATCACCGGATCGCCCGGGATCATGTGGACAAAGGCAAATGTGAGAAGGGTAATACCGATAAACGTGGGGATGACGAGTCCCAAACGTCGGAGAATAAACTGCAACA > NC_000913/3705504‑3705789 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |