Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	663,746	C→T	S73N (AGC→AAC)	dacA ←	D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	663,746	0	C	T	100.0%	314.3 / NA	81	S73N (AGC→AAC)	dacA	D‑alanyl‑D‑alanine carboxypeptidase (penicillin‑binding protein 5)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (43/38);  total (43/38)

GGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAG  >  NC_000913/663599‑663890                                                                                                                                                    |                                                                                                                                                 ggTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTgg                                                                                                                                               >  1:626606/1‑151 (MQ=255)  gTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGc                                                                                                                                              >  2:242883/1‑151 (MQ=255)     tGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAg                                                                                                                                            <  1:791327/150‑1 (MQ=255)     tGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAg                                                                                                                                            <  1:933600/150‑1 (MQ=255)      gAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGa                                                                                                                                          <  1:983530/151‑1 (MQ=255)       aGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAgg                                                                                                                                           >  2:342931/1‑149 (MQ=255)        ggAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATc                                                                                                                                        <  1:116377/151‑1 (MQ=255)             aTCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATcgcg                                                                                                                                     >  1:70708/1‑149 (MQ=255)              tCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCgcggcg                                                                                                                                  >  1:135464/1‑151 (MQ=255)              tCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCgcggcg                                                                                                                                  >  2:829714/1‑151 (MQ=255)               cAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGcggcgg                                                                                                                                 >  2:1082820/1‑151 (MQ=255)                  cGAAGAACCTTTAAACACCGGGATACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGAc                                                                                                                               >  2:630983/1‑150 (MQ=255)                    aagaaCCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATc                                                                                                                            >  2:395561/1‑151 (MQ=255)                          cTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGtt                                                                                                                      >  2:35871/1‑151 (MQ=255)                            ttAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCt                                                                                                                    >  2:285633/1‑151 (MQ=255)                             tAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCt                                                                                                                    <  1:115923/150‑1 (MQ=255)                              aaaCACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGt                                                                                                                  <  2:386226/151‑1 (MQ=255)                              aaaCACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGCGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGt                                                                                                                  >  2:762398/1‑151 (MQ=255)                               aaCACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGtt                                                                                                                 <  1:568769/151‑1 (MQ=255)                                 cacCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCg                                                                                                               <  1:285703/151‑1 (MQ=255)                                               tGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGc                                                                                                  >  2:454919/1‑150 (MQ=255)                                                ggCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCg                                                                                                <  2:338021/151‑1 (MQ=255)                                                ggCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCg                                                                                                <  1:842557/151‑1 (MQ=255)                                                    cATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGt                                                                                            >  2:367061/1‑151 (MQ=255)                                                    cATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGt                                                                                            >  2:654600/1‑151 (MQ=255)                                                        cgtcgtTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTtata                                                                                        <  1:547271/151‑1 (MQ=255)                                                        cgtcgtTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTtat                                                                                         >  2:50053/1‑150 (MQ=255)                                                            gtTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCa                                                                                    <  1:167365/151‑1 (MQ=255)                                                              tGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCaa                                                                                   >  2:804413/1‑150 (MQ=255)                                                                ccGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATc                                                                                 >  2:849940/1‑150 (MQ=255)                                                                ccGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCa                                                                                <  1:384/151‑1 (MQ=255)                                                                 cGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATc                                                                                 >  1:1017007/1‑149 (MQ=255)                                                                  gATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAgg                                                                              >  2:227657/1‑151 (MQ=255)                                                                   aTAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAgg                                                                              >  1:1056847/1‑150 (MQ=255)                                                                   aTAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGa                                                                             <  2:235809/151‑1 (MQ=255)                                                                     aGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATg                                                                           >  2:49583/1‑151 (MQ=255)                                                                     aGTGACTAAATCAGTTTCGTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGTAt                                                                            <  2:1125218/150‑1 (MQ=255)                                                                       tGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGTTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATATTCAATCAGGATGt                                                                          <  1:335070/150‑1 (MQ=255)                                                                       tGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGt                                                                          >  1:625660/1‑150 (MQ=255)                                                                        gACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTa                                                                         <  1:196014/150‑1 (MQ=255)                                                                             aaTCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTc                                                                   >  2:500120/1‑151 (MQ=255)                                                                              aTCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTcc                                                                  <  2:276308/151‑1 (MQ=255)                                                                                  gTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCAt                                                              >  2:945118/1‑151 (MQ=255)                                                                                     tCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATGTTGGTCAGGTTCGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATc                                                             <  2:874294/149‑1 (MQ=255)                                                                                      cTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGAt                                                          >  1:183991/1‑151 (MQ=255)                                                                                      cTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGAt                                                          >  1:406943/1‑151 (MQ=255)                                                                                         tAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCt                                                        <  2:391459/150‑1 (MQ=255)                                                                                         tAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTg                                                       >  2:1006446/1‑151 (MQ=255)                                                                                          aaaTTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTGTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGc                                                      <  2:806701/151‑1 (MQ=255)                                                                                              ttACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGCGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGt                                                   >  1:1110622/1‑150 (MQ=255)                                                                                                aCCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTaca                                                <  1:544619/151‑1 (MQ=255)                                                                                                aCCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTac                                                 <  1:309042/150‑1 (MQ=255)                                                                                                       ttCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCgg                                           <  2:565396/149‑1 (MQ=255)                                                                                                         cATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATc                                       <  1:376603/151‑1 (MQ=255)                                                                                                         cATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATc                                       <  2:414944/151‑1 (MQ=255)                                                                                                          aTTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATc                                       >  1:1159819/1‑150 (MQ=255)                                                                                                          aTTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCa                                      <  1:310073/151‑1 (MQ=255)                                                                                                            tGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCAt                                     >  1:686902/1‑150 (MQ=255)                                                                                                                tGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGttt                                >  2:411086/1‑151 (MQ=255)                                                                                                                  gCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTg                              <  1:1071165/151‑1 (MQ=255)                                                                                                                  gCCGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTg                              >  1:644793/1‑151 (MQ=255)                                                                                                                    cGATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGat                            >  1:50251/1‑151 (MQ=255)                                                                                                                     gATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGata                           <  2:183945/151‑1 (MQ=255)                                                                                                                     gATAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGat                            >  2:832406/1‑150 (MQ=255)                                                                                                                      aTAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGatat                          <  1:1078092/151‑1 (MQ=255)                                                                                                                       tAACGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATAtt                         >  2:704143/1‑151 (MQ=255)                                                                                                                         aCGTAACTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCa                       <  1:628945/151‑1 (MQ=255)                                                                                                                             aaCTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTc                   <  1:186439/151‑1 (MQ=255)                                                                                                                               cTGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCAt                 >  1:729598/1‑151 (MQ=255)                                                                                                                                tGGTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATc                >  1:656909/1‑151 (MQ=255)                                                                                                                                 ggTCATCATTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCg               >  2:1018182/1‑151 (MQ=255)                                                                                                                                    catcatTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCa            <  1:651561/151‑1 (MQ=255)                                                                                                                                     atcatTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAt           <  1:420877/151‑1 (MQ=255)                                                                                                                                      tcatTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtg          >  1:470603/1‑151 (MQ=255)                                                                                                                                       catTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtgt         >  1:665281/1‑151 (MQ=255)                                                                                                                                       catTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtgt         >  1:647241/1‑151 (MQ=255)                                                                                                                                       catTTTGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTGCTGTTCGGCGAGCTCTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCAtgt         <  2:963184/151‑1 (MQ=255)                                                                                                                                           ttGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgca      <  2:165042/150‑1 (MQ=255)                                                                                                                                            tGGTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgcag     >  1:957034/1‑150 (MQ=255)                                                                                                                                             ggTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTgcagca   <  1:50071/151‑1 (MQ=255)                                                                                                                                              gTCAGGTTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGcagcag  <  1:449240/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                 GGTTTGAGGAACATCAGCGAAGAACCTTTAAACACCGGGTTACCGGTGGCCCATGCGTCGTTGCCGATAGTGACTAAATCAGTTTCTTTAAATTTACCGGCTTTCATTGCCTGGCCGATAACGTAACTGGTCATCATTTTGGTCAGGCTGGCAGGATCGCGGCGGACATCTGCGTTCTGTTCGGCGAGCACTTTGCCGGAGTTATAGTCAATCAGGATGTAGGACTCCGCATCGATCTGCGGTACACCCGGGATCATAGTTTTGATATTCAGGTCATCGGCATGTGCAGCAG  >  NC_000913/663599‑663890

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑