Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 2,628,598 | 0 | G | A | 37.5% | 80.4 / 23.6 | 56 | S114L (TCA→TTA) | yfgF | cyclic‑di‑GMP phosphodiesterase, anaerobic |
Reads supporting (aligned to +/- strand): ref base G (21/14); new base A (1/20); total (22/34) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.98e-05 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAAT > NC_000913/2628450‑2628747 | gAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGa > 2:983306/1‑150 (MQ=255) aaaaGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACgttg > 1:752656/1‑151 (MQ=255) aaaGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACgttgt < 2:120385/151‑1 (MQ=255) aaGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACgttgtt > 2:1092782/1‑151 (MQ=255) acCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCgggg > 2:1134811/1‑150 (MQ=255) ccGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGa < 1:594166/150‑1 (MQ=255) ccGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAg < 1:955880/151‑1 (MQ=255) aCCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTa > 2:887727/1‑150 (MQ=255) ccAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTaaa < 1:92983/151‑1 (MQ=255) cAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAg < 2:731786/151‑1 (MQ=255) aGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGa < 1:1006576/151‑1 (MQ=255) ttCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCt < 2:474341/150‑1 (MQ=255) ctGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑Ta < 1:884915/149‑1 (MQ=255) gCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGcccc > 1:869924/1‑151 (MQ=255) aGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAAc > 2:1064329/1‑151 (MQ=255) caaaaaaTGCAGAAACCGGA‑AAAATTATCAGAAACAGCGTTGAAGAAAACACAATCTGCAAGAATAGCCGCGGGGAAATTAACCGGG‑TATCACC‑ATGGAAAACGTTGTTA‑CGCCGGGGAGAAAAGCCCCTG‑TAACCGCCCCAACAGAGaa < 1:176852/146‑1 (MQ=255) cTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGaat > 2:635009/1‑151 (MQ=255) aCAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCCCC‑ATGTAAAACGTTGTTA‑CGCCGGGGAGTAAAGCCCCTG‑TAACCGCCCCAACAGAGaatg < 2:715729/150‑1 (MQ=255) aCAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGAAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGT‑TATCACC‑ATGTAAAACGTGGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGaatga < 1:33701/151‑1 (MQ=255) aaaTGCAG‑AAACTGGAAAAATTATCAAAAAAAGCGTGGCAGAAAACACAATCTGCAAGATTATCCGCTGGGAAATAAAACGGT‑TATCCCC‑ATGTGAAACGTTGTTA‑CGCGGGGGAGAAAAGCCCCTG‑AAACCGCCCCAACAGAGaatgata < 1:395275/151‑1 (MQ=255) tGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGaatgataatg > 2:103387/1‑151 (MQ=255) agcaAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGaatgataatgat > 2:1049779/1‑150 (MQ=255) aaaCGGG‑AAAAGTATCAAAAACAGGGTTGAAGGAAACAAATTCTGCAAGAAAAGCCGTTGGGAAATTAAACGGG‑AATCCCA‑TTGTAAAAGTTTATTC‑CGCCGGGGAATAAAGCCCTTT‑TATCCGCCCCAACAGAGaatgataatg < 1:48529/145‑1 (MQ=255) aaCGGG‑AAAGTTATCGAAAACAGCGTAGCAGGAAACACAATCTGCAATAATACAGGCTGGGAAATTAACCGGG‑CATCCGC‑ATGTAAAATTTTATTA‑CGCGGGGGATTAAAGCCTGTG‑TAACCGCCCCAACAGAGAATGATAATGataata < 1:635626/150‑1 (MQ=255) aaaaGTATCAGAAACAGCTTTGCAGGAAACACAACCGCCCAAAATATACGCTGGGAAATTAAACGGG‑TATCCCA‑ATGTAAAACGTTGTTA‑CGCGGGGGAGAAAAGACCCTG‑AAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAg < 1:288338/151‑1 (MQ=255) aaaaGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCGGGGAAATTAACCGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCGGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTa < 2:908479/150‑1 (MQ=255) aaaaGTATAAGAAACAGCTTTGCAGAAAACACAATCTGCCAGAATATACGCTGGGAAATAAAACGGG‑TATCACC‑ATGTGAAACGTTTTTA‑CGCGGGGGAGAAAAGCCCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAAt < 2:259555/148‑1 (MQ=255) aaGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGcc > 2:1124143/1‑151 (MQ=255) aaGTATCAGAAACAGCGTGGAAGAAAACCCAATCGGCAAGAATATCCGCTGGAAAATAAAACGTG‑TACCCCC‑ATGTAAAGCGTTGTTC‑CGCGGGGGAGAAAAGCCCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGcc < 2:222669/151‑1 (MQ=255) cAGAAACAGCGATCAAGGAACCATATTCTTCAAAAATATACGCTGGAAAATAAAACGGG‑ATCCACC‑TTGTGAAACGTTATGC‑CGCTGGGGAGAAAAGCCCCTT‑GGCCCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGtt < 1:512492/148‑1 (MQ=255) aaaaaCACCGTGGCAGAAACCCCACTCCGCCAGAATATACGCGGGGAAATTAAACGGG‑CAACACC‑ACGTAAAACGTTTTTC‑CCCCGGGGAGAAAAGCCCTTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATg < 2:243161/149‑1 (MQ=255) aaccAGCGTGGAAGAAAACACATTATGCCAGATTATGCGGTGGAAAATAAACGGGG‑TACCACC‑TTGTAAAACGTTTTAA‑CGCGGGTGAG‑AAAAACCCTGAGAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGAt < 1:1034998/148‑1 (MQ=255) aaaCAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGa > 1:376754/1‑150 (MQ=255) aaCAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGAt > 1:481367/1‑150 (MQ=255) gggtggCAGGAAACAAATTTTGCAAGAATATACGCGGGGAAATAAAACGGT‑TATCCCC‑ATGGAAAACGTTGTTA‑GGCGGGGGAGAAAAGACCTGG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCg < 1:775766/145‑1 (MQ=255) gCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGt > 2:308615/1‑151 (MQ=255) ttGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCCCC‑ATGTAAAACGTTGTTA‑CGCCGGGGATAAAAGACCCTT‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGtt < 1:769465/149‑1 (MQ=255) ttGCAGGAAACACAACCGGCCAAAATATACGCGGGAAAATAAAACGGG‑TATCCCC‑AGGTAAAACGTTTTTA‑GGCGGGGGATAAAAGCCCTTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGtt < 1:1045823/149‑1 (MQ=255) gCAGGAAACACAATCTGCCAGAATATACCCTGGGAAATTAAACGGG‑GATCACC‑ATGGAAAACGTTGTTA‑CGCCGGGGAGTAAAGGCCCTG‑TAACCGCCCCAATAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGt > 1:373992/1‑151 (MQ=255) acaATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAAccc > 2:548414/1‑150 (MQ=255) acaAGCTGCCAGAATAGCCGCTGGGAAATAAAACGGG‑TATCACC‑ATGTAAAACGTGGTTA‑CGCTGGGGAGAAAAAACCTTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAAGTAGCCGTTAATGATAGCGTTTCGTAAAAACCCa < 1:439866/151‑1 (MQ=255) caATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAAccc > 2:14043/1‑149 (MQ=255) aaccgcccAGAATCTCCGCGAGAAAAATAAACGGT‑AATCACC‑ATGTAAAATGGTATTA‑CAACGAGAAATAAAGTCCCTA‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAg < 1:611074/144‑1 (MQ=255) tgcaataaTATAGGCT‑GGAAAATAAACGGG‑GAACCCCAATGCGAAACGTTGTTA‑CGCGGGGGAGAAAAATCCCTC‑TACCCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAATCCCAGctct < 2:349408/144‑1 (MQ=255) aaaaTATACGCGGGAAAATTAAACGGT‑TCCCCCC‑TTGTAAAAC‑TTGTTACCGCCGGGGAGAAAAACCCCTT‑AAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTg < 1:982819/145‑1 (MQ=255) ataCGCGGGAAAATTAACCGGG‑TATCCCC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGCCCCTT‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTg < 2:990852/150‑1 (MQ=255) taCGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCTTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAATCCAGCTCTGCATATTTGCg > 1:785606/1‑151 (MQ=255) taCGCGGGGAAATAAAGCGGT‑TATCACC‑ATGTAAAACGTTGTTA‑CGCCGGAAAATAAAAACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGc < 1:501045/150‑1 (MQ=255) ggAAAATTAACGGTTTATCCCC‑TTGTAAAATTTTTTTG‑CGCTGGGGTGAAAAGACCCTG‑AAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACaa < 2:982390/150‑1 (MQ=255) aaTTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATc > 1:461409/1‑151 (MQ=255) aaaCGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt > 2:1007386/1‑150 (MQ=255) ggg‑TATCACC‑ATGTAAAACTTTGTTA‑CGCGGGGGAGAAAAGCCCCTG‑TAACCGCCCCAACAGAGAATGATAACGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGc < 2:102723/151‑1 (MQ=255) gg‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGCGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg > 1:222320/1‑151 (MQ=255) gttaccccc‑ATGTAAAACTTTGTTA‑CGCCGGGGAGAAAAGCCCCTG‑CAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg < 2:913865/144‑1 (MQ=255) tcccc‑ATTTAAAACTTTGTTA‑CCCCGGGGAGTAAAGCCCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGAt < 2:1066119/146‑1 (MQ=255) cc‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTg > 2:903163/1‑151 (MQ=255) tgAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAAt > 2:762973/1‑151 (MQ=255) | GAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGG‑AAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGG‑TATCACC‑ATGTGAAACGTTGTTA‑CGCCGGGGAGTAAAGACCCTG‑TAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAAT > NC_000913/2628450‑2628747 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |