Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,628,5980GA37.5% 80.4 / 23.6 56S114L (TCA→TTA) yfgFcyclic‑di‑GMP phosphodiesterase, anaerobic
Reads supporting (aligned to +/- strand):  ref base G (21/14);  new base A (1/20);  total (22/34)
Fisher's exact test for biased strand distribution p-value = 3.98e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAAT  >  NC_000913/2628450‑2628747
                                                                                                                                                       |                                                                                                                                                       
gAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGa                                                                                                                                                        >  2:983306/1‑150 (MQ=255)
      aaaaGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACgttg                                                                                                                                                 >  1:752656/1‑151 (MQ=255)
       aaaGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACgttgt                                                                                                                                                <  2:120385/151‑1 (MQ=255)
        aaGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACgttgtt                                                                                                                                               >  2:1092782/1‑151 (MQ=255)
                  acCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCgggg                                                                                                                                     >  2:1134811/1‑150 (MQ=255)
                   ccGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGa                                                                                                                                    <  1:594166/150‑1 (MQ=255)
                   ccGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAg                                                                                                                                   <  1:955880/151‑1 (MQ=255)
                      aCCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTa                                                                                                                                 >  2:887727/1‑150 (MQ=255)
                       ccAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTaaa                                                                                                                               <  1:92983/151‑1 (MQ=255)
                        cAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAg                                                                                                                              <  2:731786/151‑1 (MQ=255)
                         aGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGa                                                                                                                             <  1:1006576/151‑1 (MQ=255)
                              ttCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCt                                                                                                                         <  2:474341/150‑1 (MQ=255)
                                  ctGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTa                                                                                                                     <  1:884915/149‑1 (MQ=255)
                                        gCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGcccc                                                                                                             >  1:869924/1‑151 (MQ=255)
                                           aGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAAc                                                                                                          >  2:1064329/1‑151 (MQ=255)
                                                  caaaaaaTGCAGAAACCGGAAAAATTATCAGAAACAGCGTTGAAGAAAACACAATCTGCAAGAATAGCCGCGGGGAAATTAACCGGGTATCACCATGGAAAACGTTGTTACGCCGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGaa                                                                                                    <  1:176852/146‑1 (MQ=255)
                                                  cTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGaat                                                                                                   >  2:635009/1‑151 (MQ=255)
                                                    aCAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCCCCATGTAAAACGTTGTTACGCCGGGGAGTAAAGCCCCTGTAACCGCCCCAACAGAGaatg                                                                                                  <  2:715729/150‑1 (MQ=255)
                                                    aCAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGAAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGTTATCACCATGTAAAACGTGGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGaatga                                                                                                 <  1:33701/151‑1 (MQ=255)
                                                      aaaTGCAGAAACTGGAAAAATTATCAAAAAAAGCGTGGCAGAAAACACAATCTGCAAGATTATCCGCTGGGAAATAAAACGGTTATCCCCATGTGAAACGTTGTTACGCGGGGGAGAAAAGCCCCTGAAACCGCCCCAACAGAGaatgata                                                                                               <  1:395275/151‑1 (MQ=255)
                                                         tGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGaatgataatg                                                                                            >  2:103387/1‑151 (MQ=255)
                                                            agcaAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGaatgataatgat                                                                                          >  2:1049779/1‑150 (MQ=255)
                                                               aaaCGGGAAAAGTATCAAAAACAGGGTTGAAGGAAACAAATTCTGCAAGAAAAGCCGTTGGGAAATTAAACGGGAATCCCATTGTAAAAGTTTATTCCGCCGGGGAATAAAGCCCTTTTATCCGCCCCAACAGAGaatgataatg                                                                                            <  1:48529/145‑1 (MQ=255)
                                                                aaCGGGAAAGTTATCGAAAACAGCGTAGCAGGAAACACAATCTGCAATAATACAGGCTGGGAAATTAACCGGGCATCCGCATGTAAAATTTTATTACGCGGGGGATTAAAGCCTGTGTAACCGCCCCAACAGAGAATGATAATGataata                                                                                      <  1:635626/150‑1 (MQ=255)
                                                                       aaaaGTATCAGAAACAGCTTTGCAGGAAACACAACCGCCCAAAATATACGCTGGGAAATTAAACGGGTATCCCAATGTAAAACGTTGTTACGCGGGGGAGAAAAGACCCTGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAg                                                                               <  1:288338/151‑1 (MQ=255)
                                                                       aaaaGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCGGGGAAATTAACCGGGTATCACCATGTGAAACGTTGTTACGCGGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTa                                                                                <  2:908479/150‑1 (MQ=255)
                                                                       aaaaGTATAAGAAACAGCTTTGCAGAAAACACAATCTGCCAGAATATACGCTGGGAAATAAAACGGGTATCACCATGTGAAACGTTTTTACGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAAt                                                                                  <  2:259555/148‑1 (MQ=255)
                                                                         aaGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGcc                                                                             >  2:1124143/1‑151 (MQ=255)
                                                                         aaGTATCAGAAACAGCGTGGAAGAAAACCCAATCGGCAAGAATATCCGCTGGAAAATAAAACGTGTACCCCCATGTAAAGCGTTGTTCCGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGcc                                                                             <  2:222669/151‑1 (MQ=255)
                                                                               cAGAAACAGCGATCAAGGAACCATATTCTTCAAAAATATACGCTGGAAAATAAAACGGGATCCACCTTGTGAAACGTTATGCCGCTGGGGAGAAAAGCCCCTTGGCCCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGtt                                                                          <  1:512492/148‑1 (MQ=255)
                                                                                aaaaaCACCGTGGCAGAAACCCCACTCCGCCAGAATATACGCGGGGAAATTAAACGGGCAACACCACGTAAAACGTTTTTCCCCCGGGGAGAAAAGCCCTTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATg                                                                      <  2:243161/149‑1 (MQ=255)
                                                                                  aaccAGCGTGGAAGAAAACACATTATGCCAGATTATGCGGTGGAAAATAAACGGGGTACCACCTTGTAAAACGTTTTAACGCGGGTGAGAAAAACCCTGAGAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGAt                                                                    <  1:1034998/148‑1 (MQ=255)
                                                                                  aaaCAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGa                                                                     >  1:376754/1‑150 (MQ=255)
                                                                                   aaCAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGAt                                                                    >  1:481367/1‑150 (MQ=255)
                                                                                       gggtggCAGGAAACAAATTTTGCAAGAATATACGCGGGGAAATAAAACGGTTATCCCCATGGAAAACGTTGTTAGGCGGGGGAGAAAAGACCTGGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCg                                                                <  1:775766/145‑1 (MQ=255)
                                                                                       gCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGt                                                               >  2:308615/1‑151 (MQ=255)
                                                                                          ttGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCCCCATGTAAAACGTTGTTACGCCGGGGATAAAAGACCCTTTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGtt                                                              <  1:769465/149‑1 (MQ=255)
                                                                                          ttGCAGGAAACACAACCGGCCAAAATATACGCGGGAAAATAAAACGGGTATCCCCAGGTAAAACGTTTTTAGGCGGGGGATAAAAGCCCTTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGtt                                                              <  1:1045823/149‑1 (MQ=255)
                                                                                            gCAGGAAACACAATCTGCCAGAATATACCCTGGGAAATTAAACGGGGATCACCATGGAAAACGTTGTTACGCCGGGGAGTAAAGGCCCTGTAACCGCCCCAATAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGt                                                          >  1:373992/1‑151 (MQ=255)
                                                                                                     acaATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAAccc                                                  >  2:548414/1‑150 (MQ=255)
                                                                                                     acaAGCTGCCAGAATAGCCGCTGGGAAATAAAACGGGTATCACCATGTAAAACGTGGTTACGCTGGGGAGAAAAAACCTTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAAGTAGCCGTTAATGATAGCGTTTCGTAAAAACCCa                                                 <  1:439866/151‑1 (MQ=255)
                                                                                                      caATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAAccc                                                  >  2:14043/1‑149 (MQ=255)
                                                                                                       aaccgcccAGAATCTCCGCGAGAAAAATAAACGGTAATCACCATGTAAAATGGTATTACAACGAGAAATAAAGTCCCTATAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAg                                                <  1:611074/144‑1 (MQ=255)
                                                                                                           tgcaataaTATAGGCTGGAAAATAAACGGGGAACCCCAATGCGAAACGTTGTTACGCGGGGGAGAAAAATCCCTCTACCCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAATCCCAGctct                                            <  2:349408/144‑1 (MQ=255)
                                                                                                               aaaaTATACGCGGGAAAATTAAACGGTTCCCCCCTTGTAAAACTTGTTACCGCCGGGGAGAAAAACCCCTTAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTg                                           <  1:982819/145‑1 (MQ=255)
                                                                                                                    ataCGCGGGAAAATTAACCGGGTATCCCCATGTGAAACGTTGTTACGCCGGGGAGTAAAGCCCCTTTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTg                                   <  2:990852/150‑1 (MQ=255)
                                                                                                                     taCGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCTTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAATCCAGCTCTGCATATTTGCg                                 >  1:785606/1‑151 (MQ=255)
                                                                                                                     taCGCGGGGAAATAAAGCGGTTATCACCATGTAAAACGTTGTTACGCCGGAAAATAAAAACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGc                                  <  1:501045/150‑1 (MQ=255)
                                                                                                                            ggAAAATTAACGGTTTATCCCCTTGTAAAATTTTTTTGCGCTGGGGTGAAAAGACCCTGAAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACaa                            <  2:982390/150‑1 (MQ=255)
                                                                                                                               aaTTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATc                       >  1:461409/1‑151 (MQ=255)
                                                                                                                                   aaaCGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCt                    >  2:1007386/1‑150 (MQ=255)
                                                                                                                                       gggTATCACCATGTAAAACTTTGTTACGCGGGGGAGAAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAACGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGc               <  2:102723/151‑1 (MQ=255)
                                                                                                                                        ggTATCACCATGTGAAACGTTGTTACGCCGGGGCGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg              >  1:222320/1‑151 (MQ=255)
                                                                                                                                         gttacccccATGTAAAACTTTGTTACGCCGGGGAGAAAAGCCCCTGCAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCg              <  2:913865/144‑1 (MQ=255)
                                                                                                                                             tccccATTTAAAACTTTGTTACCCCGGGGAGTAAAGCCCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGAt            <  2:1066119/146‑1 (MQ=255)
                                                                                                                                                ccATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTg       >  2:903163/1‑151 (MQ=255)
                                                                                                                                                      tgAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAAt  >  2:762973/1‑151 (MQ=255)
                                                                                                                                                       |                                                                                                                                                       
GAGGTTAAAAGGCATGACACCGACCAGATTTTCTCTGCTCGCCAGTAATCCTACAAATGCAGCAAACTGGAAAAGTATCAGAAACAGCGTTGCAGGAAACACAATCTGCCAGAATATACGCTGGGAAATTAAACGGGTATCACCATGTGAAACGTTGTTACGCCGGGGAGTAAAGACCCTGTAACCGCCCCAACAGAGAATGATAATGATAATAAAATTAGCCGTTAATGATAGCGTTTCGTAAAAACCCAGCTCTGCATATTTGCGGACAAAAATCCCTAAGGCGATCCCTGGCAAT  >  NC_000913/2628450‑2628747

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: