Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,443,2770AC38.2% 148.8 / 11.8 76V274G (GTA→GGA) secYpreprotein translocase membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (43/4);  new base C (0/29);  total (43/33)
Fisher's exact test for biased strand distribution p-value = 4.96e-17
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAAT  >  NC_000913/3443127‑3443423
                                                                                                                                                          |                                                                                                                                                  
gCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTa                                                                                                                                                    <  2:429661/151‑1 (MQ=255)
 cAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAc                                                                                                                                                   <  1:373958/151‑1 (MQ=255)
     cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAccccc                                                                                                                                               >  2:494061/1‑151 (MQ=255)
     cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAccccc                                                                                                                                               >  2:536879/1‑151 (MQ=255)
     cGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTAccccc                                                                                                                                               >  2:617213/1‑151 (MQ=255)
      gCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCg                                                                                                                                              >  2:860490/1‑151 (MQ=255)
       cATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGc                                                                                                                                             >  2:1043365/1‑151 (MQ=255)
        aTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGcc                                                                                                                                            >  1:1013462/1‑151 (MQ=255)
        aTAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGcc                                                                                                                                            >  2:479509/1‑151 (MQ=255)
            agTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCatat                                                                                                                                        >  1:710911/1‑151 (MQ=255)
             gTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATAtt                                                                                                                                       >  2:47651/1‑151 (MQ=255)
                acacATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCAc                                                                                                                                    >  2:174612/1‑151 (MQ=255)
                   caTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTTTTGTCAACCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACttt                                                                                                                                 >  1:503050/1‑151 (MQ=255)
                   caTAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACttt                                                                                                                                 >  1:1139416/1‑151 (MQ=255)
                      aaaGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTc                                                                                                                                >  2:2163/1‑149 (MQ=255)
                       aaGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGc                                                                                                                             >  1:200215/1‑151 (MQ=255)
                         gggttggCCCAGGCAGAAAAAACAGCGAATTTGTTGTCACCCATTCCCAACCAGTCCCCCCCCCAAACCATGACGGGAGGGCCGCCGGAAACAAAATAATACGGAAAGCGAAGATTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGc                                                                                                                             <  2:1089105/143‑1 (MQ=255)
                         gcgttcgCCCAGGCCAAAAAAACAGCAAATTTTTGGTCACCCGTTCCAAACCACTCCCCCCCCCAACCCATGCGGCGATGGTCGCGGGAAAAAAATAAATACTGGAAGCGAAGATTGCCGGAATTCCCCCCGCCATATTCACTTTCAGCgg                                                                                                                           <  1:159104/145‑1 (MQ=255)
                         gCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCgg                                                                                                                           >  2:785670/1‑151 (MQ=255)
                                cccGGGCGGAAAATACAGCAAATTTTTTGTCACCCATTCCCACCCAGTCCCCCCCCCGACCCATGACGCGATGGTCGCCGGGAACAAAATAATACTGAAAGCAAAGATTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTaaa                                                                                                                       <  2:818088/148‑1 (MQ=255)
                                         cAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtgt                                                                                                           >  1:122659/1‑151 (MQ=255)
                                          aaacaaaGCAAATTTGGTGTGAGCCAGTCCCAACAAGTCCCCCCCCCGAACCAGGACGCGGGGGTCGCCGGAAACAAAATAAGTGGGGAAGCGAAAATTGCGGGGATGCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCtg                                                                                                            <  1:341684/143‑1 (MQ=255)
                                           aagagcgagtaaTTTTTGTAAGCCCTTCCCAACCTGTCCCCCCCCCAAAACAGCCCGATATGTCCGCCGGTAAAAAAATACAACTGGAATAGATTATGGCGGGTTTCCCCCCCGCCATATTCACTTTCAGCGGTAAAtgt                                                                                                                    <  1:814138/130‑1 (MQ=255)
                                           aaTACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTgc                                                                                                         >  1:578557/1‑151 (MQ=255)
                                                    aattttttttCACCCGGTCCCAACCAGTCCCCCCCCCGAACCAGGCCGCGATGGTCGCCGGAAAAAAAATAATACGGAAAGCGAAGTTGGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCAt                                                                                                    <  2:744259/138‑1 (MQ=255)
                                                    aaaTTTTTGCCAGCCTGTCCCACCCGTCCCCCCCCCCGACCCGTGACGCGATGGTCGCGGGAAACAAAATAAGACGGGAAGCGAAGTTTGCCGGCATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                <  2:931473/151‑1 (MQ=255)
                                                    aaaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                >  1:665325/1‑151 (MQ=255)
                                                    aaaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGac                                                                                                >  2:928966/1‑151 (MQ=255)
                                                    aaaTGGTGGTCAGCCAGTTCCACCCAGTCCCGCCCCCAAACCAGGACGCGATGGTCGCGGGAAAAAAAATAATACGGGAAGGGAAGTTTGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAg                                                                                                  <  2:740966/149‑1 (MQ=255)
                                                     aaTTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGAAAATGTGTGCTCTGTGCAGCATAGac                                                                                                >  1:193645/1‑150 (MQ=255)
                                                       ttgttgTCAGCCACTTCACACCAGTCCCCCCCCCGAACAAGGACGCGATGGCCGCGGGAAACAAAATAATACTGAAAGGGAAGATGGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGacac                                                                                              <  1:793488/150‑1 (MQ=255)
                                                        tgttgtCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                             >  1:890496/1‑150 (MQ=255)
                                                          ttgtCAGCCATTCCCACCCAGTCCCGCCCCCGACCAAGGACGCGAGGGCCGCGGGGAAAAAAATAATAAGGGAAGGGAATATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                           <  1:108816/150‑1 (MQ=255)
                                                          ttgcCAGCCAGTTCAACCCCGTCCCGCCCCCAACCAAAGCCGCGGTGGCCCCCGGGAACAGAATAATACTGAAAGCGAAGTTTGCCGGAATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacg                                                                                             <  2:407835/148‑1 (MQ=255)
                                                          tggtCGGCCAGTCCAACCCGGACCCCCCCCCAACCCAGGCCGGGATGTCGGCCGGAAAAAAAATAAATCTGGAGGCGAAGAGTGCGGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACacgac                                                                                           <  1:645747/148‑1 (MQ=255)
                                                              cAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCt                                                                                      >  2:849109/1‑151 (MQ=255)
                                                              cAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCt                                                                                      >  1:457467/1‑151 (MQ=255)
                                                                gCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTg                                                                                    >  2:216534/1‑151 (MQ=255)
                                                                   aGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                 >  1:1098877/1‑151 (MQ=255)
                                                                   aGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTAGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTg                                                                                 >  1:1127156/1‑151 (MQ=255)
                                                                      tCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACg                                                                              >  2:328358/1‑151 (MQ=255)
                                                                       ccAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGt                                                                             >  2:693406/1‑151 (MQ=255)
                                                                                 aCCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGt                                                                      >  2:293196/1‑149 (MQ=255)
                                                                                    gCCCCCGAACCATGACGCGCTGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGt                                                                   >  1:651722/1‑149 (MQ=255)
                                                                                    gCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGttt                                                                 >  2:696663/1‑151 (MQ=255)
                                                                                      cccaGAACCATGACGCGAGGGTCGCCGGAAAAAGAATAATTCGGGAAGAGAAGATTGCCGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTAc                                                               <  2:312860/151‑1 (MQ=255)
                                                                                           aaCCATGACGGGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcaca                                                           <  2:206593/150‑1 (MQ=255)
                                                                                            aaccgggACGCGAGGGCCGCCGGAAAAAAAAAAATCGTGGAAGCAAAGTTGGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACca                                                             <  1:71688/142‑1 (MQ=255)
                                                                                              ccagggcgggATGGGCGCGGGAAAAAAAAAAATAGTGGAGGGGAAGTTTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAAt                                                         <  1:460359/141‑1 (MQ=255)
                                                                                                aggcggcgtgggccGCCGGAAAAAAAATAATCCTGGAAGTAAAGTTTGCGGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATgc                                                       <  1:130278/137‑1 (MQ=255)
                                                                                                 gcccgcgGTGGTCGCGGGGAACAAAAAAATGCTGGAAGCGAAGATTGCGGGTATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACcaca                                                           <  1:1056582/142‑1 (MQ=255)
                                                                                                        aTGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGAc                                              >  1:254173/1‑151 (MQ=255)
                                                                                                            cggccgaaaaCAAAAAAAGACAGGAAGGGAAGTGGGCCGGGTTACCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATg                                                        <  1:684264/129‑1 (MQ=255)
                                                                                                              gcgggaaaaaaaataatGCTGAAAAAGAAGTTTCCGGTGTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCAc                                           <  2:892052/137‑1 (MQ=255)
                                                                                                                 gggAAAAAAATAATACTGGAAGCGAAGTTGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTc                                       <  2:221943/149‑1 (MQ=255)
                                                                                                                 gggAAAAAAATAATACTGAAAGCGAAGTTTGCCGGTTTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTca                                      <  2:1078374/150‑1 (MQ=255)
                                                                                                                    aaCAGAATAATACGGAAAGCGAAGATGGCCGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTca                                      <  1:192567/147‑1 (MQ=255)
                                                                                                                     aaaaaaaaaaaaCTGAAAGCAAAAATGGCCGGCATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTcaac                                    <  2:794434/138‑1 (MQ=255)
                                                                                                                      cAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAAt                                >  2:692979/1‑151 (MQ=255)
                                                                                                                       aagaataacgggggAAGCGAAGATTGCGGTAATTCCCCCCGCCATGTTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCCCAATGCGGCGTTGACCACGCTCAACAAAt                                <  1:731528/139‑1 (MQ=255)
                                                                                                                         aataatACGGGAAGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                             <  2:1096221/151‑1 (MQ=255)
                                                                                                                          aaaaatcCTGGAAGCAAAGTTTGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATaca                             <  2:728511/148‑1 (MQ=255)
                                                                                                                             aataCTGGAAGCGAAGATTGCGGGGATCCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATacaaca                          <  2:383791/151‑1 (MQ=255)
                                                                                                                               taCTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATAcaacaa                         >  2:571230/1‑150 (MQ=255)
                                                                                                                                  tGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGa                      >  2:1127356/1‑150 (MQ=255)
                                                                                                                                    gaagaaaaaaTTGCGGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACg                   <  2:68508/142‑1 (MQ=255)
                                                                                                                                    gAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACg                   >  2:312060/1‑151 (MQ=255)
                                                                                                                                     aaGCGAAGATTGCGGGGATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGt                  <  2:458962/151‑1 (MQ=255)
                                                                                                                                        cGAAGATTGCCGGGATTACCCCCGCCATATTAACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCAc               >  1:714151/1‑151 (MQ=255)
                                                                                                                                         gAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACt              >  2:607787/1‑151 (MQ=255)
                                                                                                                                         cgaaaatGCCGGGATTTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACACCAAAGAACGTCa                <  1:928505/144‑1 (MQ=255)
                                                                                                                                             aTTGCCGGGCTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCACCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGCCCCCGCTCAACAAATCCAACACAGAACGTCCCTGc            >  1:345293/1‑149 (MQ=255)
                                                                                                                                             aTTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCa           >  1:699981/1‑150 (MQ=255)
                                                                                                                                                 ccGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac      >  2:1119916/1‑151 (MQ=255)
                                                                                                                                                 ccGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatac      >  2:312227/1‑151 (MQ=255)
                                                                                                                                                   gggATTCCCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaata       <  2:503339/148‑1 (MQ=255)
                                                                                                                                                      aTTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAaatactaat  >  2:34325/1‑150 (MQ=255)
                                                                                                                                                          |                                                                                                                                                  
GCAGACGCATAGAGTAACACATAAAGCGGTTGCCCAGGCTGCAAATACAGCGAAATTGTTGTCAGCCAGTTCCAACCAGTACCGCCCCCGAACCATGACGCGATGGTCGCCGGGAACAGAATAATACTGGAAGCGAAGATTGCCGGGATTACCCCCGCCATATTCACTTTCAGCGGTAAATGTGTGCTCTGTGCAGCATAGACACGACGACCTTGCTGACGTTTCGCGTAGTTTACCACAATGCGGCGTTGACCACGCTCAACAAATACAACAAAGAACGTCACTGCAAATACTAAT  >  NC_000913/3443127‑3443423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: