Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,622,8160GA42.1% 21.1 / 40.5 38intergenic (+75/+144)yneM/dgcZinner membrane‑associated protein/diguanylate cyclase, zinc‑sensing
Reads supporting (aligned to +/- strand):  ref base G (21/1);  new base A (0/16);  total (21/17)
Fisher's exact test for biased strand distribution p-value = 7.64e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGG  >  NC_000913/1622675‑1622956
                                                                                                                                                  |                                                                                                                                            
cGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAAc                                                                                                                                     >  1:1079555/1‑151 (MQ=255)
 gTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAAcc                                                                                                                                    >  2:687624/1‑151 (MQ=255)
    cTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGcc                                                                                                                                 >  2:1098071/1‑151 (MQ=255)
      gggAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCa                                                                                                                                <  1:258755/150‑1 (MQ=255)
                     ttctggttttctggCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGa                                                                                                                 >  2:299659/1‑150 (MQ=255)
                             ttctggCCGTTTTTTCCACCCAAAAAGGGAATAATAAAAGACGGGAAATAACCCCCCCCCAACCCGCCCCTTTTTTACTTTTTTGTACAGGGGGCCTTTTTTTTAGGACGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGc                                                                                                         <  2:1137844/150‑1 (MQ=255)
                              tctggCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCAt                                                                                                       >  2:917781/1‑151 (MQ=255)
                                tggCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATgg                                                                                                     >  2:759798/1‑151 (MQ=255)
                                 ggCCGCTTTTTCCAGCCAAAAGGGGGAGAACAAATGAGGGAAAATACCCCCCCCCCAACCCGCCCCTTTGTTACAGTTGTGTAAAGGGGGCCTTTTTTTTATGGCGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCAt                                                                                                       <  2:946541/148‑1 (MQ=255)
                                 ggCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATgg                                                                                                     >  2:412953/1‑150 (MQ=255)
                                    cgcgTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGa                                                                                                    >  2:750155/1‑148 (MQ=255)
                                     gcgTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGAt                                                                                                   >  2:703908/1‑148 (MQ=255)
                                        ttttcaacccAAAAAGGGGATAAAAAATAAACGGAAATACCCCCCCCCCAACCCGCCCCTTTTTTACGGTTGTAAACAGGGGGCCTGTTTTTTAGGAGGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGAtt                                                                                              <  2:569536/142‑1 (MQ=255)
                                        tATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGCTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGAttt                                                                                             >  1:1023607/1‑151 (MQ=255)
                                         aTTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGGGAATGCTTGCATGGATAGAttt                                                                                             >  2:699437/1‑150 (MQ=255)
                                                        gggATGAAAAATGAACGGAGATACCCCCTCCCCTACCCGCCCCTTTTTTACATTTGTGTAAAGGGGGCTTTTTTTTTATGACGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCtttt                                                                                <  2:772306/148‑1 (MQ=255)
                                                        gggATAATTAATGAAGGGAAATACCCCCCCCCCAACCCGCCCCTTTTTTCAGTTTGTGAAAAGGGGGCTTTTTTTTTATGACGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCtttt                                                                                <  2:785273/148‑1 (MQ=255)
                                                         ggtggaaaagtGAAGGGATATACCCCCCCCCCTACCCGCCCCCTTTTTACTGTTGGCAAAAGGGGTTTTGTTTTTTATGGGGAAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGc                                                                            <  1:703494/141‑1 (MQ=255)
                                                              aCTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAAc                                                                        >  1:968943/1‑150 (MQ=255)
                                                                       cGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCAtttt                                                               >  2:989082/1‑150 (MQ=255)
                                                                           gaTAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGc                                                          >  2:704536/1‑151 (MQ=255)
                                                                                 ccccccccTACCCGCCCCTTTGTTACAGTTGTTTACAGGGGGCTTTTTTTTTATGACGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGa                                                     <  2:436960/144‑1 (MQ=255)
                                                                                  cccccaccaaccctccccTTGTTTAAAGTTGGTTACAGGGGTCTTTATTTTTAGGGGGAAAAAAAAAACCCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTg                                                      <  1:418999/135‑1 (MQ=255)
                                                                                       ccTAACCGCCCCTTTGTTACATTTGTGAACAGGGGGCTTGTTTTTTAGGAGGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGaa                                              <  2:817180/151‑1 (MQ=255)
                                                                                        cTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGaa                                              >  2:550006/1‑150 (MQ=255)
                                                                                               gCCCCTTTTTAACGGTTGTGAAAAGGGGGCTTTTTTTTTATGACGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTg                                        <  2:894433/150‑1 (MQ=255)
                                                                                               gCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGac                                      >  2:838453/1‑151 (MQ=255)
                                                                                                  ccTTGTTACAGTTTTGTACAAGGGGCCTGATTTTTATGACGGGGCTAAAAAAACGCCAGTCAACCGGCGGTGAATGCTAGTACGGAAAGATTTGTGTTTTGCTTTTACGCCAACAGGGATTTTCCTCCACTGATAACGAATCGTTTactc                                    >  1:626010/1‑148 (MQ=255)
                                                                                                       ttttaaaatttttgtAAAGGGGGCTTGTTTTTTAGGCGGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGAcaca                                   <  2:542073/137‑1 (MQ=255)
                                                                                                        ttACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGc                              >  1:111429/1‑151 (MQ=255)
                                                                                                               gttttgaagaaGGGGCTTGTTTTTTATGACGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCAt                            <  1:1007457/139‑1 (MQ=255)
                                                                                                                ttgtaaaaaaGGGCCTTGTTTTTTAAGAGGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCAt                            <  2:26329/139‑1 (MQ=255)
                                                                                                                 ttgggaaaaGGGGCCTTTTTTTTTAGGAGGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATc                           <  2:563835/141‑1 (MQ=255)
                                                                                                                        agggggCCTTTTTTTTATGGCGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCa                 <  2:3807/148‑1 (MQ=255)
                                                                                                                          ggggCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGGATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCCGTTTTCTCa                 >  1:858683/1‑148 (MQ=255)
                                                                                                                           gggCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCaatg              >  2:687958/1‑150 (MQ=255)
                                                                                                                                      ttttAGGACGGAAAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTaaa     <  2:750953/150‑1 (MQ=255)
                                                                                                                                        ttATGACGGAGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGAGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACgg  >  1:409342/1‑151 (MQ=255)
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CGTACTGGGAATAATTTTATTTTCTGGTTTTCTGGCCGCGTATTTCAGCCACAAATGGGATGACTAATGAACGGAGATAATCCCTCACCTAACCGGCCCCTTGTTACAGTTGTGTACAAGGGGCCTGATTTTTATGACGGCGAAAAAAAACCGCCAGTAAACCGGCGGTGAATGCTTGCATGGATAGATTTGTGTTTTGCTTTTACGCTAACAGGCATTTTCCTGCACTGATAACGAATCGTTGACACAGTAGCATCAGTTTTCTCAATGAATGTTAAACGG  >  NC_000913/1622675‑1622956

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: