Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,603,342	0	A	C	44.4%	26.2 / 37.6	37	T509P (ACC→CCC)	hyfB	hydrogenase 4, membrane subunit
Reads supporting (aligned to +/- strand):  ref base A (19/1);  new base C (0/16);  total (19/18)
Fisher's exact test for biased strand distribution p-value = 2.33e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCG  >  NC_000913/2603193‑2603491                                                                                                                                                         |                                                                                                                                                       cgcgCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATAc                                                                                                                                                       >  1:415118/1‑151 (MQ=255)         acacacGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCg                                                                                                                                              <  2:355856/151‑1 (MQ=255)             acGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCAc                                                                                                                                          >  1:505042/1‑151 (MQ=255)              cGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACt                                                                                                                                         >  2:377554/1‑151 (MQ=255)                           caGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGa                                                                                                                             >  2:711128/1‑150 (MQ=255)                                  tGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACt                                                                                                                     >  2:31192/1‑151 (MQ=255)                                          aCGATGCGCGCCGCCTTGCTCCTGCCCGCGCCCCTGTGTTTTTTAATTGCTTTGGGTGCGGGTTGGCTGGCCCGG‑AAA‑AAAAGGCATTTGGCCC‑ATGGTTTCCCCACCCCCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCg                                                                                                             <  1:483644/151‑1 (MQ=255)                                           cGATGCTCGCCGCCGTGCTCCTCCGCGCGCCACTCTGCTTATTAATTGCGCTTAGTGCGTGTTGGCGGGAACAA‑AAA‑ATAATGCATATCCCCC‑TTGTTTTCACCACCCCCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTAccc                                                                                                              <  1:188773/149‑1 (MQ=255)                                              tgagCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTTTTAATTGCGCTTAGTGCCAGTGGACTGACACCG‑ACG‑CTCATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACTGAAGCCAGCGGAATAGAACTTGTACCCGACa                                                                                                          >  2:648945/1‑150 (MQ=255)                                                                tGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCAc                                                                                       >  1:1000397/1‑151 (MQ=255)                                                                   tCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCAcccc                                                                                    >  1:181194/1‑151 (MQ=255)                                                                       gcccctttgctttttATTTGCGTTTGGTGCCTGTTGGCTGGCACCA‑AAA‑ATAATGCTTTTTGCCC‑TTGCTTTTCCCAATCCCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTc                                                                                 <  1:271751/137‑1 (MQ=255)                                                                              tGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGt                                                                         >  1:262292/1‑151 (MQ=255)                                                                               gtttattatTGGCGTTTGGTGCCGGTGGGCGGGCACCA‑AAA‑ATATTGCTTTTGCCCC‑ATGCGTTTACAAACCCCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTg                                                                          <  1:618752/146‑1 (MQ=255)                                                                                 ttattatTTGCCCTTGGGGCGGTTTGGTTGCCACAG‑AAG‑AAAATGAAT‑TTGCCCTATGC‑TTTCCCAAATCCCCTCCCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTg                                                                       <  1:233329/149‑1 (MQ=255)                                                                                  tcttAATTGCGCTTAGCGCCAGCTGACTGGCACCG‑AAG‑ATAATGCATATTGCCC‑CTGCGATTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGAACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCCCTACTGTTGc                                                                      >  2:380458/3‑150 (MQ=255)                                                                                  tattatTGGGGCTAAGTGCCGGTGGGGGGCCA‑CG‑AAGAATATTGCATGTTCCCC‑ATGCGTTTCCCAATCCCCCC‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACt                                                                           <  1:41767/145‑1 (MQ=255)                                                                                      atttGCGTTTAGTGCTGGTTGGCGGCCCCAA‑AAA‑ATAATGCATATTCCCC‑ATGTTTTCACCAACCCCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGt                                                                   <  1:154000/147‑1 (MQ=255)                                                                                      atttGCGTTTAGTGCGGGTGGGCGGCCACCA‑AAA‑ATAATGAATTTTCCCC‑TTGCTTTTCCAAACCCCCCC‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATATACAGGTCACCCCCTCATTACTGTTGCTGtt                                                                  <  1:1007925/148‑1 (MQ=255)                                                                                           cgcTTAGTGCCAGTTGGCTGGCACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTactact                                                            >  1:645642/1‑151 (MQ=255)                                                                                           cgcTTAGTGCCAGTTGGCTGGCACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTactact                                                            >  2:447545/1‑151 (MQ=255)                                                                                               ttagtgctgTTGGCCGGGCCCGAAAG‑ATAATCAATTTTCCCC‑TTGTTTTTACCAACCCCCCC‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTg                                                           <  1:414075/141‑1 (MQ=255)                                                                                                    ccgtttGGGTGGCACCA‑AAA‑ATAATGCATTTTCCCC‑TTGCTTTTACAAACCCCCCC‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATg                                                     <  1:546801/145‑1 (MQ=255)                                                                                                          ggccggcACGG‑AGA‑ATAATTCATATTGCCC‑GGGTTTTTCCCAATCCCCCC‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTtgct                                             <  1:632983/151‑1 (MQ=255)                                                                                                           ggtgggcccaaaaG‑ATAATGCATATTGCCC‑GTGCTTTTACAAATCCCCCC‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTtgc                                              <  1:339396/139‑1 (MQ=255)                                                                                                           gctggcACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTtgctg                                            >  1:1018187/1‑151 (MQ=255)                                                                                                            cgggcACGG‑AAG‑ATAATGAATAGTCCCG‑GTTCTTTCACCACTCCCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTtgct                                             <  2:233245/147‑1 (MQ=255)                                                                                                                    g‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCtt                                   >  2:504868/1‑151 (MQ=255)                                                                                                                               gCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCtg                          >  1:589239/1‑151 (MQ=255)                                                                                                                                       gccgagtttttcaCCAACCCCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGgtgt                    <  2:52823/138‑1 (MQ=255)                                                                                                                                       ccc‑ATGCGTTCACCAACCCCCCC‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTgtggtg                     <  1:170286/148‑1 (MQ=255)                                                                                                                                        cccgtgtttttACCAACCCCCCC‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTc                   <  1:573470/142‑1 (MQ=255)                                                                                                                                          gctGCGTTTCCCAACCCCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCtgtg                        <  2:544881/141‑1 (MQ=255)                                                                                                                                            tGCGTTTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTcg              >  2:693459/1‑151 (MQ=255)                                                                                                                                                tttACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTcgcgcc          >  1:667967/1‑151 (MQ=255)                                                                                                                                                     cAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTcgcgccgcgc      >  1:340360/1‑150 (MQ=255)                                                                                                                                                        tACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCg  >  1:385459/1‑151 (MQ=255)                                                                                                                                                         |                                                                                                                                                       CGCGCAGTACACACGCTGAAGAGGCACAGGAAGTGCCAAATACGATGATCGTCGCCATGCTACTGCTCGCGGCACTCTGCGTATTAATTGCGCTTAGTGCCAGTTGGCTGGCACCG‑AAG‑ATAATGCATATTGCCC‑ATGCGTTTACCAATACCCCT‑CCCGCCACTGTCGCCAGCGGAATAGCACTTGTACCCGGCACGTTTCATACACAGGTCACCCCCTCATTACTGTTGCTGTTACTACTGGCGATGCCTTTGCTGCCTGGCCTTTACTGGCTGTGGTGTCGTTCGCGCCGCGCAGCG  >  NC_000913/2603193‑2603491

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑