Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT44.6% 50.2 / 36.4 58intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (7/24);  new base T (25/0);  total (34/24)
Fisher's exact test for biased strand distribution p-value = 8.52e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206287‑3206583
                                                                                                                                                     |                                                                                                                                                       
gtgtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCa                                                                                                                                                       <  1:234744/151‑1 (MQ=255)
  gtATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGa                                                                                                                                                     <  2:494370/151‑1 (MQ=255)
             aaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACaaaa                                                                                                                                          <  2:856727/151‑1 (MQ=255)
                  tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAATACAAAAACTgg                                                                                                                                     >  1:477432/1‑151 (MQ=255)
                  tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTg                                                                                                                                      <  2:884691/150‑1 (MQ=255)
                    ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTTAAACAAAAAAAAAAAc                                                                                                                                        >  2:182769/1‑146 (MQ=255)
                    ccATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAg                                                                                                                                   <  2:923243/151‑1 (MQ=255)
                     cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGt                                                                                                                                  <  2:688371/151‑1 (MQ=255)
                       ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAATAAAAAAACTGGAGGTg                                                                                                                                >  1:629567/1‑151 (MQ=255)
                        tCCCTGCTCTGGCGCAATCTTCATTCTTATTTTCGCGTTGATTCACTAACTGTTAATTACATATTCACGGTCGCAACTAATATAAAACCACATTTTTAGTGGTAGTTTGTGTCGGTGTCATTTTCCTTACAACTAaaaaaaaatgggattt                                                                                                                               >  2:427731/1‑141 (MQ=255)
                        tCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTg                                                                                                                                <  1:739886/150‑1 (MQ=255)
                           cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAAAAAAAAAAATGGAGTTGTCa                                                                                                                             >  2:374202/1‑150 (MQ=255)
                             gCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAAAAAAAAATGGAGGTGGCat                                                                                                                            >  1:813063/1‑149 (MQ=255)
                                 tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAACAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTAAGTAAAAAATAAAAAATGGattggtgatg                                                                                                                           >  2:1026212/1‑137 (MQ=255)
                                  ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCAtgatga                                                                                                                       <  1:466118/149‑1 (MQ=255)
                                      cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                                                 <  2:648186/151‑1 (MQ=255)
                                        atatTCATTCTTATTTTCGCTTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAgg                                                                                                               <  2:337106/151‑2 (MQ=255)
                                          attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaa                                                                                                             <  2:993514/151‑1 (MQ=255)
                                           ttcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAAATTTTTTAAAAAAAAATAAAAAATGGAGtttggatg                                                                                                                           >  2:270922/1‑130 (MQ=255)
                                             cattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGtaataa                                                                                                          <  1:909702/151‑1 (MQ=255)
                                                    aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACa                                                                                                  <  1:885380/150‑1 (MQ=255)
                                                      tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAATAAAAAAAATGGAGGTGGCATGATGAGCGGAAGGAATAAACaaaa                                                                                                   >  2:535652/1‑146 (MQ=255)
                                                              tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAGACAAATAAAAAAAATTGAGTTGCCATGATGAGTGAAAAAAATAAAAAAAAGGCAGAGaa                                                                                         >  2:757849/1‑149 (MQ=255)
                                                               gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                      <  1:687962/151‑1 (MQ=255)
                                                               gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATaa                                                                                      <  1:523334/151‑1 (MQ=255)
                                                                aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATa                                                                                       <  1:603270/149‑1 (MQ=255)
                                                                 ttCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAAAACAAAAACTGGAGGTGGCATGATGAGCGAAAGGAATAAACAACAAGCAGGGAATaa                                                                                      >  1:94484/1‑149 (MQ=255)
                                                                    aCGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAGGGAAAAAAAAAAACAACAAGCAGGGAATaa                                                                                      >  1:452893/1‑146 (MQ=255)
                                                                      gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAATTTTTTCAGGCAAAAAAAAAAAATTGAGGTTGCATGGTGATAGAAAATAATAAGGAAAAGGCAGTggagaaagttaa                                                                                >  1:321497/1‑139 (MQ=255)
                                                                             tAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAGAAATTTTTCAGACAAAAACAAAAAATGGAGTTTCCATGATGAGCGCAAGAAAAAAACAAAAAGCAGGGAATAAATTTACa                                                                               >  2:389770/1‑144 (MQ=255)
                                                                                   cATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTTAGAAAAATAAAAAAAAAGGAGGTGCCATGATGGGCGAAAATAATAAAAAACAAGCAGTGAAAAAGGTGTAAGAGAATGGCGCt                                                                  >  1:873878/1‑150 (MQ=255)
                                                                                    atatTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTCCAGCCAAATAAAAAAAATTGGGTTGGCATGATGAGGGAAAATAATAAGAAAAACGGAAGGAAAAAATTTACAGAGAATGtgg                                                                    >  2:914222/1‑145 (MQ=255)
                                                                                      atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAACAGGGGGTGGCCTGATTGGGGAAAGGAATAAACAACAAGCAGGGGATAAATTGACAGAGATTGTCGCTaa                                                                >  1:50032/1‑149 (MQ=255)
                                                                                        tCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGTGAAATTTTAAAAAAAAAACAAAAAATAGAGTTGTCCTTTTGAGGGGAAGGAAAAAGAAACAAGGAGAGGATAAGATGACAGAGAGTTGTGCTAAAttt                                                            >  2:698363/1‑151 (MQ=255)
                                                                                         cACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTCAGAAAAATAAAAAAAATGGAGTTGTTGTGATGAGGGAAAGGAAAAAAAAAAAAGCAAAGGATAAGTAGAAAGAGAATGTTGGTAAAttt                                                            >  2:26800/1‑150 (MQ=255)
                                                                                          aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCAAAAAAAAAACAAAAACAGGAGTTGTCATGGTGAGCGAAAGTAATAAAAAACAAACAATGAAAAAATTGAAAAAGATTTTCGCTAAAtt                                                             >  2:254053/1‑148 (MQ=255)
                                                                                          aCGGTGGCAAAAAATATAAAACCACATTTTTAGTGGAAGTTTGTGGCGGTGAAAGTTTTAAGACCAATAAAAAAAATGGGGTGGGCATGATGGGAGGAAGGAAAAAGCAAACAGGCGAGGAAAAAGTGGAAAAGATTGtagggcacatt                                                            >  2:305820/1‑139 (MQ=255)
                                                                                           cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAAAACAAAAAATGGAGTTGGCATGATGGGCGGAAGAAATAAACAAAAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTAcc                                                         >  1:478964/1‑151 (MQ=255)
                                                                                           cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGAATTTTTTCCGAAAAAAAAAAAAAATGGAGTTGCCCTGATGAGCGGAAGTAATAAACAAACAGCAGGGAATAAAATGAACGAGAGTGTTGCTAACttt                                                            >  2:670026/1‑148 (MQ=255)
                                                                                             gTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAAAAAAATACAAAAACTAGAGTTTGCCTGATTGGCGGAAATAATAAACAACAAGCAGTGGATAAAGTGACAGAGATTGTTGCTAACTTTATCGc                                                       >  2:205167/1‑151 (MQ=255)
                                                                                                  aaaaaaTATAAAACCACATTTTGAGTGGTAGTTTGGGGGGGTGAATTTTTCAAGAAAAAAACAAAAACGGGGGTTGCCATGAAGCGCAAAAGTAATAAGCAACAGGAAGTGAATAAGTTGACAGAGATTGTCGCTATCTTTACCGCCATg                                                   <  2:976709/150‑1 (MQ=255)
                                                                                                    aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCCGACAAATAAAAAAAATGGAGGTGCCATGATGAGCGAAAGAAATAAAAAACAGGGCGTGAATAAAATGATAGAGATTTTCGCTTACTTTTCCCCCATGAtt                                                >  1:811954/1‑151 (MQ=255)
                                                                                                    aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGAAAAAAAAAAAAACTGGAGGTGGCATGATGAGCGAAAGAAAAAAAAAAAAGGCCGGGGAAAAATTGACAGAGATTGTTGCTAACTTTACCGCCATGAtt                                                >  1:2366/1‑151 (MQ=255)
                                                                                                     aaaTATAAAACCACATTTTAAGGGGTATTTTGTGGAGGTGAATTTTTCCAGAAAAATACAAAAACGGGGTTTGCCAGGATGAGAGAAAGTAATAATCAACAGGCAGCGAATAAGTTGACAGAGATTGTCTATACCTTTGCCGCCAGGAttg                                               <  2:854424/151‑2 (MQ=255)
                                                                                                      aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAAAAATAAAAAAAATGGAGTTGGCATGGTGAGAGGAAGTAATAAACAAAAAGCAGGGGAAAAGGTGAAAGAGAATGTCGCTAACTTTTCCGCCATGAtttt                                              >  2:31678/1‑150 (MQ=255)
                                                                                                        tataAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGGAAGTAATAAACAACAAGCAGGGAATAAAGTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCt                                             >  1:193628/1‑150 (MQ=255)
                                                                                                                cacaTTTTTAGTGGTATTTTGTGGCGGTAAATTTTTCCAGACAAATACAAAACCGGGATTTGCCATGAGGAGCAAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATg                                    <  2:700259/151‑1 (MQ=255)
                                                                                                                cacaTTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTCCCGACAAATACAAAAAATGGGGTTGGCATGATGGGCGGAAGAAAAAAACAACAGGAAGTGAATAAGTTGGAAGAGATTGTCGCTAACTTTACCGCCCTGATTTTTAACCCaa                                      >  2:214957/1‑149 (MQ=255)
                                                                                                                    tttttAGTGGTAGTTTGTGGCGGGTGAATTTTTCAGACAAAAAAAAAAACTGGAGGTGGCATGATGAGGGAAAAAAAAAAAAAACAGGGAAGGGAAAAAATGACAGAGATTATTGCTAACTTTTCCACCCAGAATTTTACCCGaaagac                                  >  2:119067/1‑145 (MQ=255)
                                                                                                                      tttAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAAAAAAAAAACGGGAGTTGCCATGATGAGCAAAAGTAAAAAGCAACAGGCAGTAAATAAGTTGACAGAGATGGTCGTTAACTTTACCGCCATGATTTCTACCCGAATGCCtga                               <  2:960099/150‑1 (MQ=255)
                                                                                                                       ttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGAGCGAAAGTAATAAACAACAGGCAGGGAATAAGTTGACAGAGATTGTCGCTAACTTTACCCCCCTGATTTTTTCCCGAATGCCtg                                >  1:15563/1‑148 (MQ=255)
                                                                                                                                 tttGTGGCGGTGAATTTTTCCAGACAAATACAAAAACGGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg                     <  1:102285/149‑1 (MQ=255)
                                                                                                                                  ttGTGGCGGTGAATTTTTCCAGACAAAAACAAAAACAGGAGTTTCCCTGAGGGGGGTAAATAATAAGCAACAAGGAAAGGAAAAAGTTACAGAAATTGGCGCTAACTTTAACCCCCTTATTTTTAACCCAAAACCTGATGGCGtggggg                    >  2:869712/1‑149 (MQ=255)
                                                                                                                                   tgtgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGAt                  <  1:525990/150‑1 (MQ=255)
                                                                                                                                     tgGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATaa                >  2:709739/1‑150 (MQ=255)
                                                                                                                                       gggggtaatttttcCCAAAAAAATACAAAAACGGGAGTGGCCATGATGAGCAAAAGTAATAGGCAACGGCCATTGAATAAGTTGACGGGGATTGTCGCTAACTTGACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                    <  2:568388/136‑1 (MQ=255)
                                                                                                                                             gAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc        >  1:11580/1‑151 (MQ=255)
                                                                                                                                                tttttCCAGAAAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGAAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAg      <  1:645129/150‑1 (MQ=255)
                                                                                                                                                     ccAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa  >  2:216675/1‑149 (MQ=255)
                                                                                                                                                     |                                                                                                                                                       
GTGTATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAA  >  NC_000913/3206287‑3206583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: