Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,627,567	C→T	Q85* (CAG→TAG)	nadR →	trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,627,567	0	C	T	100.0%	247.0 / NA	63	Q85* (CAG→TAG)	nadR	trifunctional protein: nicotinamide mononucleotide adenylyltransferase, ribosylnicotinamide kinase, transcriptional repressor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/35);  total (28/35)

AATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATC  >  NC_000913/4627423‑4627714                                                                                                                                                 |                                                                                                                                                    aaTGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAgcgcg                                                                                                                                               <  2:60219/151‑1 (MQ=255)       aaaaTCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTa                                                                                                                                         <  1:869407/151‑1 (MQ=255)              aaaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGTGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAgg                                                                                                                                   <  2:199919/150‑1 (MQ=255)               aaGCCCCAGCGCTCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGtt                                                                                                                                 <  2:574370/151‑1 (MQ=255)               aaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGtt                                                                                                                                 <  1:742561/151‑1 (MQ=255)               aaGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGtt                                                                                                                                 <  2:480106/151‑1 (MQ=255)                           gcAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCat                                                                                                                     >  1:665994/1‑151 (MQ=255)                            cAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCat                                                                                                                     >  2:17049/1‑150 (MQ=255)                             aaaaGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCatat                                                                                                                   <  1:941850/151‑1 (MQ=255)                                aGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCAt                                                                                                                >  2:659311/1‑151 (MQ=255)                                  cTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCAtt                                                                                                               >  1:93514/1‑150 (MQ=255)                                   tGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTAt                                                                                                             >  2:919811/1‑151 (MQ=255)                                        gCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTGCCTTATCTAGCTCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGtt                                                                                                        <  2:385770/151‑1 (MQ=255)                                        gCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGtt                                                                                                        <  2:925811/151‑1 (MQ=255)                                        gCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGtt                                                                                                        <  2:485629/151‑1 (MQ=255)                                                ccGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGAt                                                                                                >  2:1026767/1‑151 (MQ=255)                                                    tttttGGGGATTGAGTTTCAAAGGGAGAAGAAAATGATCGGTGTTGTATTCGGTAAGTTTTAATCACTGCATACCGGACATATGTACCTTATCTAGCGTGCTTGTAGCAAGGTTGAGGAGCTGCATATCATTATGGGTTTTGACGATAc                                                                                              >  2:860467/1‑149 (MQ=255)                                                      tttGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCg                                                                                           <  2:495255/150‑1 (MQ=255)                                                       ttGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGt                                                                                          <  2:805683/150‑1 (MQ=255)                                                          gggCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGAcc                                                                                      <  2:177511/151‑1 (MQ=255)                                                          gggCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGAcc                                                                                      <  1:315383/151‑1 (MQ=255)                                                           ggCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACcg                                                                                     <  1:384093/151‑1 (MQ=255)                                                           ggCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACcg                                                                                     <  2:268357/151‑1 (MQ=255)                                                           ggCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACcg                                                                                     <  1:194883/151‑1 (MQ=255)                                                               tGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACcgcgcg                                                                                 >  2:994865/1‑151 (MQ=255)                                                               tGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACcgcgcg                                                                                 >  1:801349/1‑151 (MQ=255)                                                                gAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGCTCTACCCACTGCATACCGGACATATCTACCTTAGCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCgt                                                                                <  2:528328/151‑1 (MQ=255)                                                                 aGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCgtt                                                                               <  2:535742/151‑1 (MQ=255)                                                                   tttCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCgttgt                                                                             >  2:941216/1‑151 (MQ=255)                                                                    ttCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCgttgtt                                                                            >  2:930787/1‑151 (MQ=255)                                                                     tCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCgttgtt                                                                            <  1:322048/150‑1 (MQ=255)                                                                      ccccGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCg                                                                          <  1:430226/151‑1 (MQ=255)                                                                             agaagaAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGAc                                                                     >  2:430363/1‑149 (MQ=255)                                                                              gaagaaAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGAAAg                                                                   >  2:821671/1‑150 (MQ=255)                                                                                agaaAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGc                                                                <  1:887112/151‑1 (MQ=255)                                                                                   aaaCGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCAt                                                             >  1:377248/1‑151 (MQ=255)                                                                                      cGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTc                                                          <  1:522662/151‑1 (MQ=255)                                                                                       gATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTc                                                          >  1:1002764/1‑150 (MQ=255)                                                                                       gATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCg                                                         >  1:996026/1‑151 (MQ=255)                                                                                          cGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgca                                                       <  1:155010/150‑1 (MQ=255)                                                                                          cGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgc                                                        >  2:726640/1‑149 (MQ=255)                                                                                           ggTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCgcagc                                                     >  2:195729/1‑151 (MQ=255)                                                                                              gtCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCagcagc                                                  <  2:831445/151‑1 (MQ=255)                                                                                              gtCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCagcagc                                                  >  1:900575/1‑151 (MQ=255)                                                                                                cGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCg                                                >  1:994448/1‑151 (MQ=255)                                                                                                    ttCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCg                                            >  2:255203/1‑151 (MQ=255)                                                                                                               cTACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCg                                  >  2:1032642/1‑150 (MQ=255)                                                                                                                tACCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTc                                >  2:712818/1‑151 (MQ=255)                                                                                                                 aCCCACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCt                               <  1:801890/151‑1 (MQ=255)                                                                                                                    cACTGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCg                            <  2:471875/151‑1 (MQ=255)                                                                                                                       tGCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTg                         >  1:899142/1‑151 (MQ=255)                                                                                                                        gCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGtt                          <  2:511649/149‑1 (MQ=255)                                                                                                                        gCATACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTgg                        <  1:879590/151‑1 (MQ=255)                                                                                                                          aTACCGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGtt                      >  1:330753/1‑151 (MQ=255)                                                                                                                             ccGGACATATCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTAtt                   <  1:752266/151‑1 (MQ=255)                                                                                                                                   atatCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAAc             <  1:631872/151‑1 (MQ=255)                                                                                                                                     atCTACCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGCTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACt            <  2:924241/150‑1 (MQ=255)                                                                                                                                     atCTAACTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATCCCCGCGACCGCGCGTTGTTCGAAGACAGTGCCATGTAGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACtt           >  2:1048472/1‑151 (MQ=255)                                                                                                                                         aCCTTATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACttt          <  2:432870/148‑1 (MQ=255)                                                                                                                                            ttATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAat     >  1:73755/1‑150 (MQ=255)                                                                                                                                             tATCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAata    <  2:308453/150‑1 (MQ=255)                                                                                                                                              aTCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAatat   <  1:990939/150‑1 (MQ=255)                                                                                                                                              aTCTAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATc  >  2:588441/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                    AATGCCAAAATCAAAAGCCCCAGCGCGCAAAAGCTGGAGGCGTTGCACCGTTTTTTGGGGCTTGAGTTTCCCCGGCAGAAGAAAACGATCGGTGTCGTATTCGGTAAGTTCTACCCACTGCATACCGGACATATCTACCTTATCCAGCGCGCCTGTAGCCAGGTTGACGAGCTGCATATCATTATGGGTTTTGACGATACCCGTGACCGCGCGTTGTTCGAAGACAGTGCCATGTCGCAGCAGCCGACCGTGCCGGATCGTCTGCGTTGGTTATTGCAAACTTTTAAATATC  >  NC_000913/4627423‑4627714

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑