Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,040,075	0	C	G	50.8%	33.4 / 76.7	62	L200V (CTG→GTG)	yedY	membrane‑anchored, periplasmic TMAO, DMSO reductase
Reads supporting (aligned to +/- strand):  ref base C (9/20);  new base G (30/0);  total (39/23)
Fisher's exact test for biased strand distribution p-value = 3.58e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.09e-04

GGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAA  >  NC_000913/2039926‑2040220                                                                                                                                                      |                                                                                                                                                   ggTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGc                                                                                                                                                    <  1:1276738/150‑1 (MQ=255)    cGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGaa                                                                                                                                                <  2:1246344/151‑1 (MQ=255)            gtgCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGCAATATCCtt                                                                                                                                        >  2:567436/1‑151 (MQ=255)            gtgCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAAAATCCtt                                                                                                                                        >  2:280255/1‑151 (MQ=255)               ccGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATg                                                                                                                                     <  2:1157766/151‑1 (MQ=255)                cGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGCGAAATATcccttt                                                                                                                                      >  2:362657/1‑145 (MQ=255)                 gTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGtt                                                                                                                                   <  2:96232/151‑2 (MQ=255)                    gATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGCGGGCGGGAATATCCtctttttg                                                                                                                                  >  1:1277291/1‑142 (MQ=255)                     aTTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGGGAAAAATACTTAttt                                                                                                                                    >  2:1110226/1‑144 (MQ=255)                     aTTGGTTTTCCGCTGCACAAAGTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGaa                                                                                                                                <  1:765965/150‑1 (MQ=255)                      ttGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAAAATCCTTATGTCGAAg                                                                                                                               >  1:1115871/1‑150 (MQ=255)                              ccGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGAGGGCGGGAAATTCCCTATGTCGGAGGATTGCg                                                                                                                        >  1:941858/1‑149 (MQ=255)                                gCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAAAATCCTTTTTTCGAAGGaat                                                                                                                           >  2:646616/1‑142 (MQ=255)                                    cacaAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAAAATCCTTATGTCGAAGGGGTGTGtctccgc                                                                                                                 >  1:577382/1‑147 (MQ=255)                                    cacaAATTGCTGACGCTTGCCGATCCTACCAGCAATGCGAAGTAGGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTccac                                                                                                                 <  1:432508/150‑1 (MQ=255)                                     acaAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAAAATTCTTATTTCGAAGGATTTCGTCTcgac                                                                                                                 >  1:1151941/1‑149 (MQ=255)                                       aaaTTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGTGGAATATCCTTATGTCGGAGGGGTGTGTCTCGACGaa                                                                                                              >  1:43586/1‑150 (MQ=255)                                         aTTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAATATCCTTATTTCGAAGGATTGCGTCTcggc                                                                                                                 >  1:677041/1‑143 (MQ=255)                                             cTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAATATCCTTTTTTCGGAGGATTGCGTCTCGTCGAAGCaaag                                                                                                        >  2:581455/1‑148 (MQ=255)                                             cTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGCTGAAAAATACTTATGTCGAAGGGTTGCGTCTCGGCGAAGCaaag                                                                                                        >  2:244083/1‑148 (MQ=255)                                              tGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAATATTCTTATTTCGAAGGAGTTCGTCTCGGCGAAGGaa                                                                                                          >  2:462534/1‑147 (MQ=255)                                                     tGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAAAATTCTTTTTTTGAAAGATTTTGGCTCGACGGAGCAAAGCATcc                                                                                                   >  2:866153/1‑147 (MQ=255)                                                      gCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCCCCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTca                                                                                              <  1:17199/151‑1 (MQ=255)                                                        cGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTcaca                                                                                            <  2:169211/151‑1 (MQ=255)                                                         gAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAAAATACTTTTGTCGAAGGGGTGGGTCCCCGCGAAGCAAAGGATCCGccccc                                                                                             >  1:1130012/1‑145 (MQ=255)                                                              tACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGTGGAAAATCCTTATGTCGCAAGAGTTCGTCTCCACGAAGCAAAGCATcc                                                                                                   >  2:596558/1‑138 (MQ=255)                                                               aCCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACtgatga                                                                                     <  1:718634/151‑1 (MQ=255)                                                                 cAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTTGAAAATCCTTATGTGCGAGGGTTTCGGCTCGGCGGAGGAAAGCATccccccccacctc                                                                                        >  1:4861/1‑135 (MQ=255)                                                                    cAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTa                                                                                <  1:1409240/151‑1 (MQ=255)                                                                    cAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTa                                                                                <  2:1004597/151‑1 (MQ=255)                                                                     aaTGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAAAATACTTTTTTCGAAGGAGTGCGTCTCGACGAAGAAATGCATCCCCCCCCACTGATGACCg                                                                                  >  1:654019/1‑148 (MQ=255)                                                                          gAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAATATACTTTTGTCGAAGGATTGCGTCTCGGCGGAGCAATGCATCCGCTCACACTGATGACCGCAGggg‑t                                                                          >  1:397140/1‑150 (MQ=255)                                                                            aGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAAAATCCTTTTTTCGCAGGATTGCGGCTCGGCGAAGCAAAGCATCCGCTCACACTGATGGCCGTAGGGG‑ttt                                                                        >  2:281004/1‑150 (MQ=255)                                                                             gTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTG‑TTTAt                                                                      <  2:845326/151‑1 (MQ=255)                                                                              tATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGGGGGGTTTAAAATTCTTTTTTTGAAAGAAATGGTCCTGACGAAGTAAAGCATCCCCTCCCACCGGTTACCGTTgggggtt                                                                         >  2:1366667/1‑143 (MQ=255)                                                                               aTGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAAAATTCTTTTGTTGAAGGATTTCGTCTCGACCAAGCAATGCCTCCCCTCACACTGATGGACGTAgggggtt                                                                         >  2:895621/1‑142 (MQ=255)                                                                                 gTCGCTTTCGAAACAATTTATGCTCCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTG‑TTTATTGc                                                                   <  1:141073/150‑1 (MQ=255)                                                                                       ttCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTTGGGTGGAATATCCTTTTTTCGAAGGATTTCGTCCCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGGG‑GTTTTTGCAAGGCg                                                             >  2:905570/1‑150 (MQ=255)                                                                                         cGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTG‑TTTATGGCAAGGCGTTa                                                          <  1:570376/151‑1 (MQ=255)                                                                                          gAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAAAATACTTATTTCGAAGGATTTCGTCTCGACAAAACAAAGCATCCGCTCACACCGATGACCGTAGGGG‑GTTATGGCAAGGCggt                                                           >  2:295444/1‑147 (MQ=255)                                                                                          gAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAAAATACCTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCCCTCACACTGATGACCGTAGGGG‑TTTTTGGGAAgggggt                                                           >  1:333911/1‑145 (MQ=255)                                                                                                 tttATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAATATCCTTATGTCGAAAGAATGCGTCTCGACGAAACAAAGCATCCCCTCACACTTATGGCCGTAGGGG‑GTTTTGGCAAAGCGGTACcccc                                                     >  2:1212269/1‑148 (MQ=255)                                                                                                      gCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGCGGAATATCCTTATGTCGAAGGATTTCGTGCCGACGAAGCAACGCCTCCGCCCCCACTGATGAGCGGCGGGG‑TTTTTTGCAAGGGGGTACCCCCCCaaaa                                               >  2:707451/1‑149 (MQ=255)                                                                                                        aCCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAATATTCTTTTGTTGGAGGATTTGGTCTCGGCGGAGCAAAGCCTCCCCCCACACTGATGACCCTTGGGG‑GTTTTTGCAAAGCGGTACCGCCCCaaaaaag                                            >  2:1309910/1‑147 (MQ=255)                                                                                                         ccGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAATATTCTTCTGTCGGAGGGGTGCGTCTCCGCGCAGAAAAGCATCCCCTCCCACCCCTGCACGGAGGGG‑GTTTTGTCGAAGGGTTACCACCCCAAAATg                                             >  1:777080/1‑148 (MQ=255)                                                                                                             aaCAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGAAATATTCTTATGTCGAAGGATTGTGGCTCGACGAAACAATGCATCCGCCCACACTGATGACCCTAGGGG‑TTTTTGGCAAGGCGGTACCCCCACAAAAAAGGGCGcc                                      >  2:536320/1‑151 (MQ=255)                                                                                                              aCAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGGTGGAATATTCTTATGTTGGAGGGATGCGTCTCGGCGGAGCAATGCATCCGCCCACACTGATGAACGGAGGGG‑GTTTTGGCAAGGCGTTACCGCCCCAAAATgg                                            >  1:138499/1‑144 (MQ=255)                                                                                                                aGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGGAAAATTCTTTTGTTGAAGGATTTCGTTTCGGCGAAGAAAATCATCCCCTCCCCCCTGTGAGCGTAGGGGTTTTTTGGGAAGGGGTTACC‑CCCCAAAAAGgcggg                                        >  1:1405648/1‑144 (MQ=255)                                                                                                                   tGCCAGGCCAGAAGGCCCGCTTTATCGGCGGTGGGCTAAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTg                                  <  2:120577/149‑1 (MQ=255)                                                                                                                     ccaggccagCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCAAGGGATGGCGTCTCGACGAAGCAATGCACCCGCTCACACTGAGGACCGTGGGTG‑TTTATGGAAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGa                               <  2:950078/150‑1 (MQ=255)                                                                                                                      caggccagCAGGACCGCTTTATCGGCGGTGGGCGGAAATAACCTTCTTTCTAAGGATATCGTCTCCCCGAACAAAAGCATCCGCTCCCCCTGATGAACGTAGGGG‑TTTTTTGGAAAGGGTTACCCCCCCACAATAGGGCGCCgggggg                                >  2:1268872/1‑144 (MQ=255)                                                                                                                      caggccagCAGGACCGCTTTATCGGCGGGGGGCTGAAAAATCCTTATGTCGAAGGGTTTCGTCTCGACGCAGCAAAGCATCCGCTCACACTGATGACCGTAGGGG‑TTTATGGCAAGGCGGTACCGCCACAAAAAGGCGCGCCGGGGCg                                >  1:366568/1‑148 (MQ=255)                                                                                                                       aggccagCAGGACCGCTTTATCGGCGGTGGGCGGCAATAACCTTTTTTCGAAGGGATGCGTCTTGGCGGAGGAATGCATCCTCTCCCACTTAATACCGGAGGTG‑GTTTTTGCAAGGCGTTTCCCACACAAAAAAGCGGGGCGGGGCGACTg                            >  1:603319/1‑151 (MQ=255)                                                                                                                        ggccagCAGGACCTCTTTACCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCCCGCCGAAGCAATCCATCCGCCCACCCTGATGACCGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGa                           <  2:666822/151‑1 (MQ=255)                                                                                                                        ggccagCAGGACCGCTTTATCGGCGGTGGGGTGGAATATACTTTTTTCGAAGGATTTTGTCTCGGCGAAGGAATGCCACCCCTCCCACTGAAGGGCGGCGGGG‑TTTTTGGCAAAGCGGTAACGCCACCAAAAGGCCCGCCgg                                    >  1:1215662/1‑142 (MQ=255)                                                                                                                           cagcagGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATtg                        <  2:416009/151‑1 (MQ=255)                                                                                                                                ggACCGCTTTATCGGCGGTGGGTTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTGGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGc                     <  2:577484/149‑1 (MQ=255)                                                                                                                                   ccGCTTTATCGGCGGTGGGCTAAAATTTCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTgg                <  1:1096575/151‑1 (MQ=255)                                                                                                                                     gtttgaTCGGGGGTGGCCAAAAATATCCTTATGCCGAAGGTTTGCTGCTCGACGAAGCAATGAATCAGCACCCCCCGTTGCACGTAGGTG‑TTTATGGCAAGGCGTTGCCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGa               <  1:1310446/145‑1 (MQ=255)                                                                                                                                        ttATCGGCGGTGGGTTGAAATTTCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCACCCGCTCACACTGATGACCGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAat            <  2:1130217/150‑1 (MQ=255)                                                                                                                                             ggcggTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCt      >  1:1327082/1‑151 (MQ=255)                                                                                                                                             ggcggTGGGCTGAAATATCCTTATGTCAAAGGATTGCGTCTCGACGAAGCAATGCTCCCGCTCACACTGATGCCCGTAGGTT‑TTTATGGCAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCt      <  2:3018/151‑1 (MQ=255)                                                                                                                                                 gTGGGTTAAAATATCCTTATGTCGAAGGTTTGCGTCTCGACGAAGAAATGCATCCGCTCACACTGATGACGGTAGGTG‑TTTATGGCAAGGCTTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTaa  <  2:132040/151‑1 (MQ=255)                                                                                                                                                      |                                                                                                                                                   GGTCGATGGTGGTGCCGTGGATTGGTTTTCCGCTGCACAAATTGCTGGCGCTTGCCGAACCTACCAGCAATGCGAAGTATGTCGCTTTCGAAACAATTTATGCACCGGAACAGATGCCAGGCCAGCAGGACCGCTTTATCGGCGGTGGGCTGAAATATCCTTATGTCGAAGGATTGCGTCTCGACGAAGCAATGCATCCGCTCACACTGATGACCGTAGGTG‑TTTATGGCAAGGCGTTACCGCCACAAAATGGCGCGCCGGTGCGACTGATTGTGCCGTGGAAATATGGCTTTAA  >  NC_000913/2039926‑2040220

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑