Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,646	0	T	G	46.6%	113.0 / 18.2	74	V316G (GTA→GGA)	yeiI	putative kinase
Reads supporting (aligned to +/- strand):  ref base T (5/34);  new base G (34/0);  total (40/34)
Fisher's exact test for biased strand distribution p-value = 1.34e-15
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTG  >  NC_000913/2252499‑2252796                                                                                                                                                    |                                                                                                                                                            aGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATTGTTTTATGGCGGGCCTGTtata                                                                                                                                                          <  1:263477/151‑1 (MQ=255)   cAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGgata                                                                                                                                                          >  2:503142/1‑149 (MQ=255)          ttCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTTCTGACCGCGCCATCGCATACGACATTATACATTTTTTTTGCTTACGATTTTTTTATGGCGGGCCTGGTATATATCT‑TTc                                                                                                                                                 <  1:113689/150‑1 (MQ=255)          ttCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAATT‑TTCt                                                                                                                                                >  2:319848/1‑151 (MQ=255)            cAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAAAT‑TTCttt                                                                                                                                              >  1:1021232/1‑150 (MQ=255)             aGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGa                                                                                                                                             <  1:1253923/151‑1 (MQ=255)             aGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATTGGt‑tttttt                                                                                                                                              >  1:843133/1‑149 (MQ=255)                aaCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGCT‑TTTTTgt                                                                                                                                             >  1:1012127/1‑147 (MQ=255)                   tgtttgttTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGCATATAGAT‑TTTTTTCAGGAt                                                                                                                                        >  1:105969/1‑150 (MQ=255)                    gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGAt                                                                                                                                        <  2:547466/149‑1 (MQ=255)                       tgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGGTATAGTT‑TTTTTTTAGGGTACAg                                                                                                                                    >  1:326237/1‑150 (MQ=255)                         tttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCCGGGTATAGAT‑TTTTTGTAGGATACAGgtt                                                                                                                                 >  2:1182457/1‑151 (MQ=255)                          ttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAACt‑tttttttagggtaaaggttt                                                                                                                                >  2:1166801/1‑136 (MQ=255)                          ttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATTACT‑TTCTTTTAGGGTAAAGtttt                                                                                                                                >  1:1386217/1‑150 (MQ=255)                           tATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGTTTTTAGCT‑TTTTTTAAGGGTACAAttt                                                                                                                                 >  2:465916/1‑149 (MQ=255)                             tttGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGTGATTGCT‑TTTTTTTAGGATACAGttt                                                                                                                                 >  2:1188052/1‑147 (MQ=255)                               tGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGGTATAGAT‑TTTTTTTAGGAGACatgtt                                                                                                                                 >  1:843669/1‑141 (MQ=255)                               tGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTCGGATATTGCT‑TTTTTTTTGGATACAGttt                                                                                                                                 >  1:575512/1‑145 (MQ=255)                                 cccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGAT‑TTTTTTTAGGGTAAAGtt                                                                                                                                  >  1:606635/1‑142 (MQ=255)                                  ccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGGTATAGCt‑tttttttagggtaaaggtt                                                                                                                                 >  2:393772/1‑128 (MQ=255)                                    gATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGTTATATCT‑TTTTTTAAGGAAACAGgtt                                                                                                                                 >  2:1356050/1‑140 (MQ=255)                                      tGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGATTAGAT‑TTCTTTTAGGATACAGTTTTCGCGGCAGCG‑cc                                                                                                                   >  1:1069226/1‑151 (MQ=255)                                       gAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑ccc                                                                                                                  <  2:804176/151‑1 (MQ=255)                                          tCAGTTTATTGCAGCGAAAAGGATGGAGAACTATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAGAT‑TTTTTTTAGGGTACAGTTTTCCCGGCAGCG‑ccc                                                                                                                  >  2:1037964/1‑148 (MQ=255)                                          tCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATATTT‑TTCTTTGAGGATACAGtttt                                                                                                                                >  1:1328589/1‑134 (MQ=255)                                                    gCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAg                                                                                                      <  1:960019/150‑1 (MQ=255)                                                           aaaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGGTATAGAC‑TTTTTGTAGGAAAAAGATTTCGCGGCAGAGCCCCGtttttggtgggccgcgcg                                                                                               >  2:467556/1‑137 (MQ=255)                                                              ggATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATAGCT‑TTTTTTTAGGGTAAAGATTTCGCGGCCGCG‑CCCCCTTTTCGGGTGCCCGCGC‑gg                                                                                            >  1:718805/1‑149 (MQ=255)                                                                aTGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATAACT‑TTTTTTTAGGATACAGTTTTCCCGCCAGCG‑CCCGCTTTTTGGTAGcc                                                                                                    >  2:1324666/1‑140 (MQ=255)                                                                aTGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAACT‑TTCTTTTAGGATACAGTTTTCCCGACAGCG‑CCCCCTTTTCGGGAGCCCGCGC‑gg                                                                                            >  2:1380524/1‑147 (MQ=255)                                                                 tGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATTTAGCT‑TTTTTTGAGGAAAAAATTTTCGCGACCGCG‑CCCCCTTTTCGGGAGCCTTCGC‑GGCCAtt                                                                                       >  2:1234545/1‑151 (MQ=255)                                                                      aaCAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTcgcg                                                                                  <  2:664620/151‑1 (MQ=255)                                                                         aaTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCgcgcgc                                                                               <  2:625086/151‑1 (MQ=255)                                                                          aTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGCT‑TTCTTTGAGGAGTCAGTTTCCGCGCCCCAG‑CCCGTTTTGTGGTGGCCTGCGG‑CGCGACTTCGCGCGcc                                                                              >  1:47409/1‑151 (MQ=255)                                                                           tttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCT‑TTTTTTAAGGGTACAGTTTTCGCGACAGCG‑CCCCGTTTTTGGGAGCCCGCGC‑GGCAATTTTTCGCGcc                                                                              >  2:1268431/1‑150 (MQ=255)                                                                            ttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCa                                                                             <  1:593083/150‑1 (MQ=255)                                                                             tttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAg                                                                            <  1:261920/150‑1 (MQ=255)                                                                                gCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGc                                                                        <  1:728504/151‑1 (MQ=255)                                                                                 cTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGGTATAGCA‑TTTTTTTAGGGTACAGGTTCCGCGACCGCG‑CCCGCTTTGCGGTAGGCCGCGC‑GGCAATTTTGCGCGcc                                                                              >  1:854416/1‑144 (MQ=255)                                                                                 cTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAACT‑TTTTTTTAAGATACAATTTTCCCGCCCGCG‑GCCGCTTTTCGGGGGCCCCCGC‑GGGGATTTTTCGCGccc                                                                             >  1:1293089/1‑144 (MQ=255)                                                                                  tGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCT‑TTTTTTGAGGGTAAAGGTTTCGCGACACCGCCCCCTTTTTCGGT‑GGCTCCGC‑GGGCATTTTGCGccccc                                                                             >  1:280398/1‑143 (MQ=255)                                                                                   gACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑c                                                                    <  1:911515/151‑1 (MQ=255)                                                                                    aCCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑t                                                                  <  2:729742/151‑1 (MQ=255)                                                                                      cgcgCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTa                                                                <  2:883478/151‑1 (MQ=255)                                                                                        cgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTaaa                                                              <  1:1419672/151‑1 (MQ=255)                                                                                          ccAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAaaca                                                            <  2:990181/151‑1 (MQ=255)                                                                                          ccAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAaaca                                                            <  1:214569/151‑1 (MQ=255)                                                                                          ccAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGTAATAACT‑TTTTTGAAGGAGACCAATTTTGTGGCAACG‑CCCGGTTTTCGGTTGGCAGCGC‑GGGGAATTTGCGCGCCCGCGGGAG‑cattaa                                                               >  1:916895/1‑144 (MQ=255)                                                                                                cATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAaccct                                                       <  2:159068/150‑1 (MQ=255)                                                                                                  tACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATTGCTGTTTTTTTAGG‑TAAAAATTTTGCGGCAGCG‑CCCGCTTTTCGGTTGGCCGCGC‑GGGCCATTCGGTGGCCGGCGGCAG‑C‑TTataaa                                                            >  1:766424/1‑139 (MQ=255)                                                                                                   aCGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAACT‑TTTTTTAAGGGTACAATTTTCTCCACAGCG‑CCCCCTTTTCGGGAGCCTGCGC‑GGCCATTTTTCGCGCCCCCGGCCGCC‑TTAAAAAacccaccc                                                    >  2:522778/1‑147 (MQ=255)                                                                                                    cGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGGTATAGCT‑TTTTTTTAGGAGAAAGGTTTCGCGGCAGCG‑CCCGTTTTTCGGGAGCCTGCGC‑GGGGCATTTTCGCGCCCGCGGCAG‑CGTTAAAAAacccacccc                                                   >  1:1368233/1‑146 (MQ=255)                                                                                                         gTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTg                                             <  2:773782/151‑1 (MQ=255)                                                                                                         gTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTg                                             <  2:970054/151‑1 (MQ=255)                                                                                                          tAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTTGGGTTTTTCT‑TTTTTTTATGGTACAGTTTTTTCGGCCGCC‑CCCCCTTTTTGGTGGCCTGCGC‑GGCGATTTTGCGCGCCCGCGGCCG‑C‑CTAAAAAacccctccccct                                                >  1:867117/1‑139 (MQ=255)                                                                                                            gACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCg                                          <  2:35631/151‑1 (MQ=255)                                                                                                             aCAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGa                                         <  1:852540/151‑1 (MQ=255)                                                                                                              cAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGTTATAGGT‑TTTTTTTAGGATAAAGATTTCGCCACCACG‑CCCGCTTTTCGGGTGCCCTCGC‑GGGCATTTCTGGCGCCAACGGGCG‑G‑GTATAAAACCCCACCCCGTCTGcc                                           >  2:1391478/1‑148 (MQ=255)                                                                                                               aGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATa                                       <  1:1032517/151‑1 (MQ=255)                                                                                                                    tGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAAcgc                                   <  1:698633/150‑1 (MQ=255)                                                                                                                    tGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGCT‑TTCTTTAAGGATAAAGTTTCCGCGGCAGCG‑CCCGGTTTTCGGTAGCCTGCGC‑GGCCATTTTGCGCGCCCGCGGCCG‑C‑TTAAACAACCCTACCCCGTCTGCCGATAAcgc                                   >  2:275975/1‑150 (MQ=255)                                                                                                                         cTGACGATGGTTTTATGGCGGGCCTTGTATATAACT‑TTTTTTTAAGGTACAATTTTCTCGGCAGCG‑CCCGTTTTTCGGGGGGCTGCGC‑GGCAATTTTTCTCGCCAGCGGCGG‑C‑TTTAACAACCCCCCCCTCTCTGCCGCTAACACCCttt                              >  1:190694/1‑150 (MQ=255)                                                                                                                          tGACGATGGTTTTATGGCGGGCCCGGGATATATCT‑TTCTTTTAGGGTACAGTTTTCGCGACAGCG‑CCCGCTTTTTGGTAGCCTGCGCGGGCAATTT‑GCCCGCCCGCGGCAG‑C‑TTTAACAACCCCACCCCGTCTGCCGGTAACgcgc                                 >  1:1407554/1‑146 (MQ=255)                                                                                                                           gACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTc                             <  1:509041/149‑1 (MQ=255)                                                                                                                           gACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCAt                           <  2:287690/151‑1 (MQ=255)                                                                                                                               aTGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGt                       <  1:319798/151‑1 (MQ=255)                                                                                                                                 ggTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTg                      <  2:105983/150‑1 (MQ=255)                                                                                                                                    tttATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCa                   <  2:1151153/150‑1 (MQ=255)                                                                                                                                       aTGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATg                <  1:1267290/150‑1 (MQ=255)                                                                                                                                       aTGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATg                <  1:1187834/150‑1 (MQ=255)                                                                                                                                         ggCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGgt              <  2:1021415/150‑1 (MQ=255)                                                                                                                                                 tGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACa        <  2:215201/148‑1 (MQ=255)                                                                                                                                                  ggTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATg     >  1:775982/1‑150 (MQ=255)                                                                                                                                                  ggTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGt    >  2:895696/1‑151 (MQ=255)                                                                                                                                                   gTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGtt   <  1:465831/151‑1 (MQ=255)                                                                                                                                                   gTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGtt   <  1:484150/151‑1 (MQ=255)                                                                                                                                                    tatataGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTg  >  2:587132/1‑151 (MQ=255)                                                                                                                                                    |                                                                                                                                                            AGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCT‑TTCTTGAAGGATACAGTTTCCGCGACAGCG‑CCCGTTTTGCGGTAGCCTGCGC‑GGCAATTTCGCGCGCCAGCGGCAG‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATGTTG  >  NC_000913/2252499‑2252796

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑