| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,875 | 0 | A | T | 43.4% | 62.1 / 22.7 | 55 | N272K (AAT→AAA) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base A (18/12); new base T (0/23); total (20/35) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.39e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATG‑TGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCG > NC_000913/357730‑358014 | aCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGCCCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAt‑tttt < 1:1296992/151‑1 (MQ=255)aCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAt‑tttt > 1:349978/1‑151 (MQ=255) gTAGGCCTGATAAGCGTTGCGCATCAGGCAATTCCAGCCGCTGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑ccg < 2:1078182/150‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACACCCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGcaaca < 2:592377/149‑1 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGcaacaa > 1:734887/1‑150 (MQ=255) aTAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACaaa < 1:1281186/151‑1 (MQ=255) aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAAc > 2:943476/1‑151 (MQ=255) aGCGTAGCGCATCAGGCAATTCCAGCCGCATACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAccc > 1:805933/1‑151 (MQ=255) aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAAcc < 2:1419831/151‑1 (MQ=255) cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAAccgc > 1:568882/1‑151 (MQ=255) cGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAAccg < 2:1100193/150‑1 (MQ=255) gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAAccgcc > 2:206636/1‑151 (MQ=255) gcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTtc > 2:1297746/1‑150 (MQ=255) aTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCagtag > 1:1034086/1‑150 (MQ=255) ccACCCGCAGCCTTGTGTCAGCGGCTACCGTAATCCATTCCCCCCAACACCCGCCCTTTTACCCAACGCCATGTGCAAAAATCCCTTCGCGGCGGCTGTCGGCCAGCTTT‑TTTC‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGc < 2:963330/151‑1 (MQ=255) ccGCAGCCCGGTGTCGGGGGCTCCCGTGATTCATTCCCGCCAACACCCGCGCTTTTACCCACCGCCATGTGAAAAAATCCCTTCGCGGCGGCTGCCTGCCAGCTTT‑TTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCggg < 2:9727/150‑1 (MQ=255) ccGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggcg > 1:441115/1‑149 (MQ=255) aGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCggggg > 1:691357/1‑147 (MQ=255) ccTGTGTCGGCGGCTCCCGTAATTCATTCCCCCCAACACCCGCGCATTTATCCACCGCCATGTAAAAAAATGCCTTCGCGGCGGCTGTCTGCCAGTTTT‑TTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGc < 2:761798/145‑1 (MQ=255) tgtgTCGGCGGCTACCGTTTTTCTTTCCCGCCAACACCCGCTATTTTACCCACCCCCATGTGAAAAAATGCTTTCGCGGCGGCTGTCTGCCAGTTTT‑TTTC‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCa < 2:671532/144‑1 (MQ=255) tgggtCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTCCTCCAACGCCATGTGCAAAAATGCCTTCGCAGCGGCTGTCTGCCAGCTGTATTTT‑CTGCCG‑GATACAACCTCCGCTCCCTTCAGCAGCGGCGTGGcccccgga > 1:50112/4‑143 (MQ=14) tgtCGGCGGCCCCCGTTTTTCATTCCCGCCAACACCCCCGCATTTACCCACCGCCATGTGCAAAAATCCCTCCGCGGGGGCTGTCTCCCAGTTTT‑TTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAaga < 2:774533/145‑1 (MQ=255) gtCAGCCGCTCCCTTGATTCTTTCCCCCCAACACCCCCCCTTTCACCCACCGCGATTCCAAAAAATCCCTCGGGGGCGCCTGTCGCCCTGTTTT‑TTTC‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATa < 1:1379383/150‑1 (MQ=255) aGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGCGAG‑aaaaaaatt > 1:923980/1‑145 (MQ=255) ggggttcccttGATTCTTTCCCCCCAACACCCCCGCTTTTACCCACCCCAATTTGAAAAAATGCCTTGGCGGCGGCTTTCCGCCAGCTTT‑TTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAaga < 2:30248/130‑1 (MQ=255) gcgcccccctgttttCTTTCCCCCCAACACCCGCCCTTTTACCCACCCCCTTTTCAAAAAGTGCCTCCGCGGGGGCGGTCTCCCTGTTTT‑TTCC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATa < 1:644287/133‑1 (MQ=255) gCGGCTCCCGTGATTCATTCCCCCCAACACCCGCCCTTTTACCCAACCCCTTGTCAAAAAATCCCTCCGCGGCGGCTGTCTCCCACTTAT‑TTTT‑CCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAAt < 2:1065892/145‑1 (MQ=255) gCGGCTACCGAGTTTCATTCCCCCCAACACCCCCGCTTTTACCCACCCCCATGAGCAAAAAGGCCTCGGCGGCGGTTTTCTGCCAGTTTT‑TTTC‑CCGCCAAAAAAAAGCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCttt < 1:153701/151‑1 (MQ=255) cGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCACAAATGCCTTCGCGGCGGCTGTCTGCCAGCTCT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCCAGCG‑GGATATCttt > 2:591839/1‑150 (MQ=255) gCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggcggg > 1:1377573/1‑132 (MQ=255) cggtttttCTTTCCCCCCAACACCCGCGTTTTTACCCACCGCCTTGTGAAAAAAGCCTTTGGGGGCGGTTGTCGGCCGGTTTT‑TTTC‑CCGCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGcc < 2:564581/143‑1 (MQ=255) ccGTGATTCTTTCCCCCCAACACCCGCGCATTTACCCACCCCCTTGTGAAAAAATCCTTCCGCGGCGGCTTTCTCCCAGTTTT‑TTTC‑CCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGcc < 2:885229/149‑1 (MQ=255) ccGTGATTCATTCCCGCAAACACCCGCGCATTTACCAACCCCCATTTAAAAAAAGGCCTGCGCGGGGGTTGTCTGCCAGTTTT‑TTTC‑CCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAg < 2:948807/147‑1 (MQ=255) ttgttcccttcccGCCAACCCCCGCGATTTTCCCCACCCCCTTGTAAAAAATTGCCTCCGCGGGGGCTGCCGCCCGGTTTT‑TTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGcc < 1:659285/136‑1 (MQ=255) ttGATTCATTCCCCCCAACCCCCCCCCTTTTCCCCACCCCCATGTGAAAAAATCCCTTCGCGGCGGTTTCCTCCCATCTAT‑TTTC‑CCCCCGCAACAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGcc < 1:354610/146‑1 (MQ=255) ttCTTTCCCCCCAACACCCGCCTTTTTATCCACCGCCTTGTGAAAAAATGCCTTCGCGGCGGTTGTC‑GCCAGGTTT‑TTTTCCCGCCAAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGcc < 1:726199/143‑1 (MQ=255) ttccccccAACACCCGCCCATTTACCCACCCCCATGTGAAAAAAGGCCTCCGCGGCGGCTTTCTGCCAGTTAT‑TTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCAtgt < 1:715639/141‑1 (MQ=255) ccccccaccaCCCGCGCATTTACCCACCGCCGTGGGCAAAAATCCCTCCGCGGCGCCTGTCGCCCACTTAT‑TTTC‑CCGCCGAAAAAAAGCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTtgtgt < 1:934235/146‑1 (MQ=255) ccccaccaCCCGCCCTTTTCCCCACCGCCGTGGGAAAAAATGCCTCGGGGGCGGCTGTCTGCCAGTTTT‑TTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGc < 1:572821/149‑1 (MQ=255) gcgcATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAG‑AAAAAACTTTTAACCCGCCCTGTTTTGTggggatag > 2:189260/1‑139 (MQ=37) ttACCCACCCCCATGTAAAAAAATGCCTCCGCGGCGGCTGTCTCCCACCTTT‑TTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAAc < 2:648829/150‑1 (MQ=255) cAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGCGAG‑AAAAAAAATTTAACCCGCCCTATTGTGTggggggcgcggct > 1:294597/1‑128 (MQ=21) acccccATGTGAAAAAATCCCTCCGCGGCGGTTGTCTCCCATTTTT‑TTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATG‑Tgg < 2:35312/147‑1 (MQ=255) taaaaaaaTCCTTGCGGGGGGGCTGTCTGCCAGTTTT‑TTTC‑CCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATG‑TGGAAAGGGaa < 2:1191629/147‑1 (MQ=255) aaaagagCCTCCGCGGGGGCTTTCGGCCAGTTTT‑TTTC‑CCCCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATG‑TGGAAAGGGAAGt < 1:643199/143‑1 (MQ=255) aaaaaGGCCTCCGCGCCGGTTGCCTGCCATTTTT‑TTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATG‑TGGAAAGGGAAGTg < 2:471829/150‑1 (MQ=255) aaaacccttccggggcgccTTTCGGCCAGTAAT‑TTTG‑CCCCAGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATG‑TGGAAAGGGAAGTgcg < 1:985711/138‑1 (MQ=255) aaaaTGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAGAAAAAAGATTTAA‑CCCCCCCTGTTGTGTGGGGATCGCCGCCGGGTACAAAG‑TGgggaggggaggggg > 2:131680/1‑147 (MQ=255) gCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGCG‑AAATAACTTTAAACCCGTCCTGTTTTGTGGGGATCGCTGCTGGTAACAATG‑TGGAAAGGGGAGGggggcg > 1:1041862/1‑149 (MQ=255) ccTTCGCGGCTGCTGTCTCCCAGCTAT‑TTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATG‑TGGAAAGGGAAGTGCGGCGAAt < 2:39694/151‑1 (MQ=255) cTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGAG‑AAAAAAAATTTAAACCGCCCTGTTGTGTGGGGAACGCCGCTGGGAAAAATGTTGGAAAGGGagggggggg > 2:1412534/1‑139 (MQ=255) cTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGAGAAAAAAATTTTAACCCCCCCTTGTT‑TGTGGGGATCGCCCCTGGTAACAAAA‑TGGGAAGGGGAgagggggg > 2:177824/1‑140 (MQ=255) ggggcggtTGTCTGCCATTTTT‑TTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATG‑TGGAAAGGGAAGTGCGGCGAATCa < 2:994444/146‑1 (MQ=255) cTGCCAGTTTT‑TTTC‑CCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATG‑TGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACcg < 2:793042/149‑1 (MQ=255) ccAGCTTT‑TTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATG‑TGGAAAGGGAAGTGCGGCGAATCAGCTCCAGa < 2:735011/142‑1 (MQ=255) | ACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTCGCGGCGGCTGTCTGCCAGCTAT‑TTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATG‑TGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCG > NC_000913/357730‑358014 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |