Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,423,290	0	A	C	37.9%	71.2 / 15.4	67	intergenic (+96/+133)	yhdZ/rrfF	putative amino acid ABC transporter ATPase/5S ribosomal RNA of rrnD operon
Reads supporting (aligned to +/- strand):  ref base A (13/28);  new base C (25/0);  total (39/28)
Fisher's exact test for biased strand distribution p-value = 8.26e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCC  >  NC_000913/3423143‑3423438                                                                                                                                                      |                                                                                                                                                     gcATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCCT‑CCCGcccccc                                                                                                                                                   >  1:1046895/1‑151 (MQ=255)    tCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCCG‑Ccccccccccccc                                                                                                                                                >  1:118310/1‑151 (MQ=255)      cTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑Ccccccccccccccg                                                                                                                                              >  1:710199/1‑149 (MQ=255)       tAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGGGGCCTG‑CCCGCCCCCCCCCcgg                                                                                                                                             >  2:891601/1‑151 (MQ=255)        aaaTCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCggg                                                                                                                                             <  1:1322423/150‑1 (MQ=255)        aaaTCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGc                                                                                                                                            <  1:43154/151‑1 (MQ=255)           tCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGGGTCCGT‑CCCCCCCCCCCCCCGGcc                                                                                                                                           >  1:8006/1‑149 (MQ=255)           tCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGGCCCG‑CCCCCCCCCCCCCCGGcc                                                                                                                                           >  1:486339/1‑149 (MQ=255)            cAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGGCCTG‑CCCCCCCCCCCCCCGGcc                                                                                                                                           >  1:1274303/1‑148 (MQ=255)               agCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGGGCCT‑CCCGCCGccccc                                                                                                                                                 >  2:146393/1‑137 (MQ=38)                   tACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGGCCCT‑CCCCCCCCCCCCCCGGCCGTTTCtt                                                                                                                                    >  2:1233267/1‑148 (MQ=255)                     cGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGGCTCCCT‑CCCCCCACCCCCCcgggc                                                                                                                                           >  2:589192/1‑137 (MQ=255)                     cGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCCG‑CCCCCCCCCCCCCCGGCCggt                                                                                                                                        >  1:530159/1‑140 (MQ=37)                      gAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGGGTCCTG‑CCCCCCCCCCCCCCGGCCGGTGCtt                                                                                                                                    >  1:334738/1‑145 (MQ=255)                          gCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGTTGTCCTG‑CCCGCCACCCTCCCGGGCGGTGCttttcaa                                                                                                                               >  1:1161632/1‑141 (MQ=255)                           cATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTT‑CCCGCCACCCTCCCGGCCCGTGCTTCGCCACGtt                                                                                                                           >  1:930686/1‑149 (MQ=255)                           cATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCCCCCCCCCCCGGGCCGTTGGTTTGCaaactt                                                                                                                           >  2:1178032/1‑145 (MQ=25)                            aTTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGGCCTG‑CCCCCCACCCTCCCGGCCGGTTCTTTGCAACGtt                                                                                                                           >  2:37384/1‑148 (MQ=255)                              ttttATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTGTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCaaa                                                                                                                       <  2:1052345/150‑1 (MQ=255)                                  aTCGCAGTTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCg                                                                                                                   <  2:318741/150‑1 (MQ=255)                                  aTCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCCCCCCCCCCCCGGCCGGTTCTTTCCCACGTTCAAATTCGc                                                                                                                  >  1:142596/1‑151 (MQ=21)                                  aTCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGGCCTG‑CCCGCCCCCCTCCGCCCCGCTGCTTCTCAACGTTCAAAAcccc                                                                                                                  >  2:840833/1‑149 (MQ=255)                                    cGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTc                                                                                                                <  1:902262/151‑1 (MQ=255)                                       aGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCcg                                                                                                             <  1:530172/151‑1 (MQ=255)                                         gTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCcggc                                                                                                           <  1:785082/151‑1 (MQ=255)                                          tAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGGGGCCTT‑TCCGCCCACCTCCGGGCCGTTGGTTTTCAACGTTCAAATACACTCCcg                                                                                                             >  2:629987/1‑148 (MQ=255)                                              ccATTAATTGAATGTTAGTTCTAAAAGCAAAAGGGCCATCCTTTCGGAGGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAt                                                                                                       <  2:410846/150‑1 (MQ=255)                                              ccATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAtt                                                                                                      <  1:626153/151‑1 (MQ=255)                                              ccATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAtt                                                                                                      <  2:1320703/151‑1 (MQ=255)                                              ccATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGAt                                                                                                       <  2:527340/150‑1 (MQ=255)                                               cATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTT‑GCCGCCACCCTCCGGGCCGGTTGTTCGCAACCTTCCCATACCCCCCCGCCGGagt                                                                                                      >  2:1212537/1‑148 (MQ=18)                                                       gAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGCGGGGGGGCCGC‑CCCCCCCCCCCCCGGGCGGTTGC‑TCTCGAAGTTCAAATTCGCTCCCCGCGGGGTTGtgcct                                                                                               >  2:147622/1‑145 (MQ=255)                                                        aaTGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCCCCCCCCCCCCGGCCGCTTCTTCTCCACATTCAAAACCCCCCCCCGCGGGttt                                                                                                     >  1:870260/1‑142 (MQ=18)                                                         aTGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCg                                                                                           <  1:1033429/151‑1 (MQ=255)                                                             tAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGga                                                                                        <  1:779046/150‑1 (MQ=255)                                                                   gAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGGGGCCTT‑CCCGCCCCCCCCCCGGGCGGTTTTTCTCAAAGTTCAAATCCCCCCCCCCCGGGATTGTGCTTCTCcgggg                                                                                       >  2:1217259/1‑143 (MQ=255)                                                                   gAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATCGC‑GGGGGTCCCG‑CCCGCCACCCCTCGCGCCGCTTCCTCCCTACGCTCAAATACACTccc                                                                                                              >  2:1401013/1‑122 (MQ=255)                                                                    aaaaGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTC‑CCCCCCCCCCTCCCGGCCGGTGCCTTGCAACGCTCAAATCCGCCCCCCGCGGGGTTTTCCCTCTCTGGagag                                                                                     >  1:397833/1‑146 (MQ=17)                                                                     aatgCAAAAAGGCCATCCGTCAGGATGGCCTTCTGCTTAATTTGATGCCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCCCCCCCCCGGCCCGTGGTTTGCCACGTTCAAATCCGCCCCCCGCGGGTTTTTTCTtctccagcgagcg                                                                                   >  1:602561/4‑138 (MQ=11)                                                                       aGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGGCCTG‑CCCGCCACCCTCCCGGCCGGTGCTTCTCCACGTTTAAATCCCCTCCCCGCGGGTTTTTTCTACTCCGGGGAGTGTTcctc                                                                             >  2:415761/1‑148 (MQ=255)                                                                          aaaaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGGCCTG‑CCCCCCCCCCCCCCCGCCGGTGCTTTGCCACGTTTAAATCCCCTCCCCGCGGGTTTGTCCTTCTCCGGGGAGGGttgtccgcc                                                                          >  2:115769/1‑144 (MQ=255)                                                                           aaaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGGGGCCTG‑GCCGCCCCCCTCCCGGGCGGTGCTTCCCCAAGTTCAAATACCCCCCCCCCGGGTTTGTTCTTCTTTGGGGAGGGTTTACCgcc                                                                          >  2:1289146/1‑148 (MQ=255)                                                                            aaaGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTAGGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTc                                                                                                                          <  2:1014975/101‑1 (MQ=255)                                                                              aGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCCG‑CCCCCCCCCCCCCGGGCCGGTTCTTCGCAACGTTCAAATACCCCCCCCGCGGGTTTGTTCTACTCCGGAGAGAGTGTACCGccaaa                                                                       >  2:1199716/1‑150 (MQ=14)                                                                               ggCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGacaaaca                                                                     <  2:249231/151‑1 (MQ=255)                                                                               ggCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGGCCTG‑CCCCCCCCCCCCCCGGCCGTTGCTTCGCAACCATCAAAACCGCCCCCCGCGGCGTTTTTCTACTCTGGGGAGGGTTTCCCCacaaa                                                                       >  1:77380/1‑149 (MQ=255)                                                                                     ccTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGAt                                                               <  1:1261766/151‑1 (MQ=255)                                                                                     ccTTTCGGATGGCCTTTCGCTGGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCGCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGAt                                                               <  1:279717/151‑1 (MQ=255)                                                                                      cTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGGGGCCTG‑CCCCCCACCCCCCCGGCCGGTTCTTCGCCACGTTCCAATACCCCCCCCGCGGGTTTGTTCTACTTCGGGGAGTGTTTACCCACAAaaaacaaa                                                                >  1:1230452/1‑147 (MQ=255)                                                                                       tttCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGGGTCCCG‑CCCCCCCCCCCCCCGGCCGTTGCTTTGCAACCCTTAAATCCCCCCCCCGCGGGTTTGTCCTACTCTGGGGAGTGTTCACCCCCAAACAAAAGATa                                                              >  1:551034/1‑150 (MQ=255)                                                                                       tttCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCCCCCCGCCGTTGCTTCGCAAAGTTTTAATCCGCGCCCGGCGGATTTGTCCTACCCGGGAGAGTGTTCTCCCACAAACAACAGATa                                                              >  1:127535/1‑150 (MQ=14)                                                                                          cGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTT‑CCCGCCACCCCCCGCGCCCTTGCTTCTCAACGTTTCAATCCGCCCCCCGCGGGTTTTTTCTTCTCGGGGGAGGGTTCACCGACAAACAACAAATaaaa                                                           >  1:1208991/1‑150 (MQ=14)                                                                                           ggATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACa                                                         <  1:933536/151‑1 (MQ=255)                                                                                           ggATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATACGCTCCCCGCGGGTTTGTCCTACTCGGGGGAGGGTTCACCGACAAACAACAGATaaaa                                                           >  1:948545/1‑149 (MQ=255)                                                                                             aTGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACaaa                                                       <  2:869999/151‑1 (MQ=255)                                                                                                 ccTTTCGCTGGATTGGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGc                                                   <  2:1394525/151‑1 (MQ=255)                                                                                                    ttCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGGGGGCTG‑CCCCCCCCCCACCCGGCCGCTGGTTTGCAACGTTCAAATCCCCCCCCCGCGGGGTTTTCCTTCTCGCGGGAGAGTTTCCCCACAAAAAACAGAGAAAACAAAAGGacc                                                 >  2:653364/1‑150 (MQ=255)                                                                                                      cGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAg                                               <  2:791652/150‑1 (MQ=255)                                                                                                          tgatttgatGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCt                                            <  1:1172088/149‑1 (MQ=40)                                                                                                              ttgatGTCTGGCAGTTTATGGC‑GGGCGGCCCGCCCCCCCCCCCCCCGGGCGGTGGTTTCGAAAGTGTCACA‑CCGCCCCCCGCCGGTTTGTTCTACCCGGGAGAGGGGTCCCCCCCACACCACAaaaaaaaaaaaaagacc                                                 >  2:560847/1‑135 (MQ=255)                                                                                                               tgatGTCTGGCAGTTTATGGC‑GGGCGGCCTG‑CCCGCCCCCCCCCCGGGCGTTGCTTCGCAACGTTCAAATCCCCCCCCCGCGGGATTTTCCTACTCCGGGGAGGGTTTACCGCCAAACAAAAAATAAAAAAAAAAGGCCAGTCTTcc                                         >  1:1050004/1‑147 (MQ=2)                                                                                                                   gTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAg                                  <  2:1102389/150‑1 (MQ=38)                                                                                                                   gTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGc                                 <  2:709992/151‑1 (MQ=37)                                                                                                                      tGGCAGTTTATGGC‑GGGCGTGCCG‑CCCGCCCCCCCCCCGGCCCTGGTTTTTCGACGTTCAAATACCCTCCCCGCGGGCTTGTTCTTCTCGGGGGAGTGTTTACCCACAAACAACAGAAAAAAAAAAAAACCCCGTCCTCCGACCGAGccct                              >  2:727760/1‑149 (MQ=2)                                                                                                                       ggCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCtt                              <  2:486171/150‑1 (MQ=35)                                                                                                                                   c‑gggcgTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTg                  >  1:236274/1‑150 (MQ=38)                                                                                                                                     gggcgTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGAt                <  2:535462/151‑1 (MQ=38)                                                                                                                                         gTCCTG‑CCCGCCACCCTCCTGGCCGTTGCTTCGCATCGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGACAGTGTTCACCGACAAACAACAGATGAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTTTTTTATTTGagg               <  2:832582/148‑3 (MQ=14)                                                                                                                                         gTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCt            >  1:974726/1‑151 (MQ=40)                                                                                                                                                    ccACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTccc  <  1:559832/151‑1 (MQ=40)                                                                                                                                                      |                                                                                                                                                     GCATCCTAAATCAGAGCGTACGAGGGCATTTTTATCGCAGGTAATCCATTAATTGAATGTTAGTTCGAAAAGCAAAAAGGCCATCCTTTCGGATGGCCTTTCGCTTGATTTGATGTCTGGCAGTTTATGGC‑GGGCGTCCTG‑CCCGCCACCCTCCGGGCCGTTGCTTCGCAACGTTCAAATCCGCTCCCGGCGGATTTGTCCTACTCGGGAGAGTGTTCACCGACAAACAACAGATAAAACAAAAGGCCCAGTCTTCCGACTGAGCCTTTTGTTTTATTTGATGTCTGGCAGTTCCC  >  NC_000913/3423143‑3423438

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑