Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913599,5760TG46.8% 104.8 / 34.8 80V288G (GTG→GGG) cusAcopper/silver efflux system, membrane component
Reads supporting (aligned to +/- strand):  ref base T (5/37);  new base G (35/2);  total (41/39)
Fisher's exact test for biased strand distribution p-value = 1.20e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACC  >  NC_000913/599426‑599725
                                                                                                                                                        |                                                                                                                                                     
aaaCGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGggt                                                                                                                                                       <  1:705242/151‑1 (MQ=255)
   cGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGgggggg                                                                                                                                                     >  1:465311/1‑150 (MQ=255)
    gCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGggggggg                                                                                                                                                    >  1:1423957/1‑149 (MQ=255)
       cgacgaCTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATc                                                                                                                                                <  2:828998/151‑1 (MQ=255)
       cgacgaCTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATc                                                                                                                                                <  2:624493/151‑1 (MQ=255)
        gacgacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATcc                                                                                                                                               >  1:297705/1‑151 (MQ=255)
           gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTg                                                                                                                                             >  1:242654/1‑150 (MQ=255)
           gacTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGc                                                                                                                                            <  2:649171/151‑1 (MQ=255)
             cTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTTCg                                                                                                                                           >  2:1119493/1‑150 (MQ=255)
             cTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGGCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGggggtgggcc                                                                                                                                               >  2:814152/1‑142 (MQ=255)
              tttAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCg                                                                                                                                           <  1:273639/149‑1 (MQ=255)
                tAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGAAGGGGGGGGGagtctacgacc                                                                                                                                        >  1:934425/1‑140 (MQ=255)
                 aaTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTcccccgaacc                                                                                                                                       >  2:595117/1‑142 (MQ=255)
                  aTCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCgg                                                                                                                                     <  2:33725/151‑1 (MQ=255)
                    cacaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGc                                                                                                                                    <  1:1014546/150‑1 (MQ=255)
                     acaTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCaa                                                                                                                                  <  1:1437870/151‑1 (MQ=255)
                      caTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCaaa                                                                                                                                 <  1:1086297/151‑1 (MQ=255)
                       aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCaaaa                                                                                                                                <  1:1199436/151‑1 (MQ=255)
                       aTCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCttaggccccggcaa                                                                                                                                 >  2:1429228/1‑137 (MQ=255)
                          gTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACg                                                                                                                             <  1:1446620/151‑1 (MQ=255)
                             ttAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTTCGGTCCGGCAAAAACGccc                                                                                                                          >  2:666971/1‑151 (MQ=255)
                              tAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGccc                                                                                                                          <  1:677872/150‑1 (MQ=255)
                               aaaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAACGccc                                                                                                                          >  1:1187807/1‑149 (MQ=255)
                                aaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGCGGGGGGGGGTCCCTCGGTCCCGGCAAAACCGcc                                                                                                                           >  2:702327/1‑148 (MQ=255)
                                aaaGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCCAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGTGTGGTGGTGATCCTGCGATCCGGCAAAAACGCCCgag                                                                                                                       <  2:938817/151‑1 (MQ=255)
                                    ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGt                                                                                                                   <  1:614841/151‑1 (MQ=255)
                                    ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGGCCCGCGATCCCGCCAAAAAGCCCgaga                                                                                                                      >  1:1365437/1‑148 (MQ=255)
                                    ccAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCgag                                                                                                                       >  1:92689/1‑147 (MQ=255)
                                     cAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGGGAAGTg                                                                                                                  >  1:273836/1‑151 (MQ=255)
                                         gAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGAGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCCAGAAGGGATcg                                                                                                              >  1:1407645/1‑151 (MQ=255)
                                          aaaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATc                                                                                                               >  1:111771/1‑149 (MQ=255)
                                           aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGGGATCCGGGAAAAAAGCCCggggagggaatg                                                                                                              >  2:125823/1‑138 (MQ=255)
                                           aaaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATcgcc                                                                                                            <  2:1105872/151‑1 (MQ=255)
                                            aaTGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTTCTGCGATCCGGGAAAAAAGCCCgaggaggg                                                                                                                  >  1:453661/1‑142 (MQ=255)
                                                     cccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGATCCGCGCTCCGGCAAAAAAACACGAGAAGTGTGCGccggcggg                                                                                                      >  2:1244090/1‑145 (MQ=255)
                                                      ccGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGTGGGGTTCCTTCGATCCGGCAAAAAAGCCCGAGGAGGGGTCGGCGGCGGGAAgg                                                                                                  >  2:340150/1‑150 (MQ=255)
                                                       cGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCACAAACGCCCGAGAAGTGATTgccgcc                                                                                                         >  2:123557/1‑142 (MQ=255)
                                                        gTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGGGATCCTGCTCTCCGGCAAAAACGCCCCGGAAGCGATGGGCGCCGTGAAGGAc                                                                                                >  1:534065/1‑150 (MQ=255)
                                                           tATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCCAGAAGAGATCTCCGCCGGGAAGGAaaaa                                                                                             >  1:1088678/1‑150 (MQ=255)
                                                            aTCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGCAGTGGCGGGCGGGGGGGGGATCCTGCGCTCCGGCCAAAAAGCCCGCGAAGTGGATGCCGCCCTGAAGGACAAAc                                                                                            >  1:491067/1‑150 (MQ=255)
                                                                gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACt                                                                                           <  1:1398867/147‑1 (MQ=255)
                                                                gcgcGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCCGCAAAAAAGGCCGAGAAGTGGACGCCGGCCGGAAGGGGAAAcc                                                                                           >  2:621010/1‑146 (MQ=255)
                                                                 cgcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCCGGGCGGTGATCCTGCGGTCCCGCAAAAAACCCCCAGAGCGGGTCCCCCCCGGGAAGGACAAACtcg                                                                                         >  2:179219/1‑146 (MQ=255)
                                                                  gcgATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGGCAAAAAGCCCGAAAAGGGATAGCCGCCCGGAAGGACAAACTGGaaa                                                                                      >  1:1379443/1‑150 (MQ=255)
                                                                    gATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGc                                                                                   <  2:372506/151‑1 (MQ=255)
                                                                     aTGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCCGGAAAAACGCCCGCGAAGGGATCGCCGCCGTGAAGGACAAACTGGAAAAGc                                                                                   >  1:972618/1‑150 (MQ=255)
                                                                      tGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCCGCGATCCGGCAAAAAACCCCGAGAAGGGATCGGCGCCGTGGAGGACAAACTGGAAAAGc                                                                                   >  2:1044690/1‑149 (MQ=255)
                                                                        ttGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGa                                                                                <  1:20350/150‑1 (MQ=255)
                                                                         tGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGaa                                                                               <  1:118900/150‑1 (MQ=255)
                                                                           cGAAGGTCCCGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGACGTGGCGGGCCGGGGGGTGGCCCTGGGGTGCGGCCAAAACACACCAGAAGAGATCGCCGCCCGGGAGGGCAAAACGGAAAAGCTGaaa                                                                              >  2:905469/1‑149 (MQ=255)
                                                                           cGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAACGCCCGAGGAGGGATCGCCGCCCTGAAGGACAAACTGGAAACGCTGaaa                                                                              >  1:670476/1‑149 (MQ=255)
                                                                           cGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGAGCCTGGGATCCGGCAAAAAAGCCCGAGAAGGGAACGCCGCCGTGAAGGGCAAAAAGGGAACCGTGaaa                                                                              >  1:1178368/1‑148 (MQ=255)
                                                                            gAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagt                                                                           <  2:71536/151‑1 (MQ=255)
                                                                             aaGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagta                                                                          <  1:517069/151‑1 (MQ=255)
                                                                             aaGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGAACGCCCCCGTGGAGGACAAACTGGAAAAGCTGGAAag                                                                            >  2:1281141/1‑149 (MQ=255)
                                                                              aGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAagtag                                                                         <  1:1059934/151‑1 (MQ=255)
                                                                                 tCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAGGACAAACTGGAAACGCTGAAAAGTAGTc                                                                       >  1:1387122/1‑150 (MQ=255)
                                                                                  ccAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTg                                                                     <  1:730301/151‑1 (MQ=255)
                                                                                         ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAg                                                              <  1:1357151/151‑1 (MQ=255)
                                                                                         ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGGGGTCCTGCGATCCGGCAAAAAAGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAACTGGAAACGCCGAAAAGTAGTCTGCCGGa                                                                >  2:1304767/1‑149 (MQ=255)
                                                                                           cccGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGc                                                            <  1:766133/151‑1 (MQ=255)
                                                                                                    gcgcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGAt                                                    <  2:465148/150‑1 (MQ=255)
                                                                                                      gcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAAAGCTGAAAAGTAGTCTGCCGGAAGGCGTGGGGATa                                                   >  1:639011/1‑149 (MQ=255)
                                                                                                      gcCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGGGAAGGACAAACTGGAAACGCTGAAAAGTAGGCTGCCGGGAGGCGGGGGGGTAGt                                                 >  1:675222/1‑151 (MQ=255)
                                                                                                           ggaaTTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGGCCTGCGATCCGGGAAAAACGCCCGCGAAGGGGTCGCCGCCGTGGAGGACAAACTGGAAAAGCTGAAAAGTAGTTTGCCGGGAGGCGTGGGGATAGTTaaa                                             >  2:1294579/4‑148 (MQ=255)
                                                                                                             cATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTacaaca                                          <  2:560697/151‑1 (MQ=255)
                                                                                                             cATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAAAGCCCCAGAAGGGATCGCCGCCGTGTAGGGCAAAATGGGAAAGCTGAAAAGGAGTCTGCCGGGAGGGGGGGGGATAATTtcaac                                           >  1:168618/1‑150 (MQ=255)
                                                                                                              aTTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGATCCTGCGATCCGGCAAAAAAGCCCGAGAAGTGATCGCCGCCGCGAAGGACAAAATGGAAAAGCTGAAAAGTAGTCTGCCGGAAGGGGGGGAGATAGTTacaaca                                          >  1:944126/1‑150 (MQ=255)
                                                                                                                tGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGTGTTCCTGCGCTCCGGCGAAAACGCCCCAGAAGAGATCCGCGCCGTGAAGGACAAACCGGAAACGCTGAAAAGAAGGCTGCCGGAAGGCGGGGCGGTAGGTacaacaaa                                        >  1:667890/1‑148 (MQ=255)
                                                                                                                    gAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATc                                   <  1:595302/151‑1 (MQ=255)
                                                                                                                    gAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATc                                   <  1:353522/151‑1 (MQ=255)
                                                                                                                         aaaCGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGAt                                    <  1:680331/145‑1 (MQ=255)
                                                                                                                         aaaCGGCGAAGACGAGGGGGCGGGAGGGGGGGTGATCCTGCGATCCGGCAGAAGCGCCCGAGAAGTGATCGGCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTATAGATAGTTACAGCATACGATCGca                                <  2:852902/149‑1 (MQ=255)
                                                                                                                           aCGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagcca                            <  1:21218/151‑1 (MQ=255)
                                                                                                                             ggcgaaggcgaaGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGcagccagc                          <  2:1101566/151‑1 (MQ=255)
                                                                                                                             ggcgaaggcgaaGTGGCGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGGGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGGAGTCTGCCGGAAGGCGGGGAGATAGTTACAACATACGATCGcagccag                           >  1:1040915/1‑150 (MQ=255)
                                                                                                                                 aaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCAt                      <  1:360307/151‑1 (MQ=255)
                                                                                                                                   ggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTg                    <  1:1247630/151‑1 (MQ=255)
                                                                                                                                     cgaagTGGGGGGCGGGGGGGTGATCCTGCGATCCGGCAAAAGCGCCCGAGAAGTGATGGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGAc                  <  2:1249757/151‑1 (MQ=255)
                                                                                                                                     cgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGAc                  <  1:81381/151‑1 (MQ=255)
                                                                                                                                           ggcgggcgGGGTGGTGACCCTGCGATCCGGCAAAACCGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCgcgc             <  2:1178009/150‑1 (MQ=255)
                                                                                                                                             cgggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCAt          <  2:111751/151‑1 (MQ=255)
                                                                                                                                               gggcgggGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCAt          <  2:187789/150‑1 (MQ=255)
                                                                                                                                                     gggTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACa     >  1:745248/1‑150 (MQ=255)
                                                                                                                                                        tggtgATCCTGCGATCCGGCAAAACCGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAAcc  <  1:397838/150‑1 (MQ=255)
                                                                                                                                                        |                                                                                                                                                     
AAACGCTCGACGACTTTAATCACATCGTTTTAAAAGCCAGTGAAAATGGCGTGCCCGTTTATCTGCGCGATGTTGCGAAGGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGCGTGGAGATAGTTACAACATACGATCGCAGCCAGCTCATTGACCGCGCCATCGACAACC  >  NC_000913/599426‑599725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: