Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,966,751 C→T A112V (GCG→GTG)  rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,966,7510CT98.8% 300.0 / ‑4.3 80A112V (GCG→GTG) rhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (42/37);  total (43/37)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.75e-01

TTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGT  >  NC_000913/3966605‑3966898
                                                                                                                                                  |                                                                                                                                                   
tttCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTgctg                                                                                                                                                 >  2:65539/1‑151 (MQ=255)
      ccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGaa                                                                                                                                            >  2:412627/1‑150 (MQ=255)
          tCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGCGAACGCTATTTTGTGCTGCTGAAAGtt                                                                                                                                        >  2:1232397/1‑150 (MQ=255)
           ccGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTa                                                                                                                                       >  1:796316/1‑150 (MQ=255)
                  cAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGtt                                                                                                                               >  1:1374265/1‑151 (MQ=255)
                   aGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGtt                                                                                                                               >  1:1428586/1‑150 (MQ=255)
                     ctcctcCCTCGCCGGTCCTGATGACTTCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACg                                                                                                                                    <  1:1303294/143‑1 (MQ=255)
                      tCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACt                                                                                                                           >  1:887785/1‑151 (MQ=255)
                       cctccctcCCCGGTCCTGATGACATCTACTTTCCCCCTAGCAAAACCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTaa                                                                                                                             <  1:1182736/148‑1 (MQ=255)
                       ccTGCCTCGCCGCTCCTGATGACATCTACGTTTCCCTAACCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTaa                                                                                                                             <  1:1235780/148‑1 (MQ=255)
                       ccTCCCCCGCCGGCCCTGATCACCTCTACTTTCCCCCTCCCCAAACCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTa                                                                                                                              <  2:1211970/147‑1 (MQ=255)
                           ccTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGa                                                                                                                       <  1:1144742/150‑1 (MQ=255)
                              cGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACaa                                                                                                                    >  1:854846/1‑150 (MQ=255)
                                 cGGTCCTGATGACATCTACGTTTCCCCAAGACAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCt                                                                                                                >  2:626233/1‑151 (MQ=255)
                                  gttCCTGATCACATCTACGTTCCCCCTAGCCAAATCCGCCGTTTCACCCTCCGCACTGTTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAcc                                                                                                                 <  2:1064908/147‑1 (MQ=255)
                                    tCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGa                                                                                                              >  1:777803/1‑150 (MQ=255)
                                       tgatgaCATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAAc                                                                                                          >  2:245041/1‑151 (MQ=255)
                                            ccaacTACGTTCCCCCAGCCCAAACCCCCCTTTTCATCCCCCGCCCGGGTGATACCATCTCGGTTAGGATGCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGccc                                                                                                      <  2:1323160/146‑1 (MQ=255)
                                             cATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGCGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGc                                                                                                    >  1:426623/1‑151 (MQ=255)
                                             cATCTACGTTCCCCCAACCAAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACg                                                                                                         <  1:805556/146‑1 (MQ=255)
                                                    gTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaa                                                                                              >  1:877664/1‑150 (MQ=255)
                                                    gTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACaaa                                                                                              >  2:1121945/1‑150 (MQ=255)
                                                     ttccccctcccaaaaCCCGCCGTTTCAACCCCCCCTCTGGTAATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaa                                                                                                  <  2:1052442/133‑1 (MQ=255)
                                                     ttcccccTGGCCAAACCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaacaa                                                                                               <  1:1307931/145‑1 (MQ=255)
                                                     ttcccccTAGCCAAATCCGCCGTTTCACCCCCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGc                                                                                                    <  1:1150113/140‑1 (MQ=255)
                                                      ttCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATc                                                                                           >  2:392935/1‑151 (MQ=255)
                                                        ccccccccaaaaTCCGCGTTTCCACCCCCCGCACTGCTCATCCCTTCTCTGGTACGATCCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaac                                                                                                 <  2:300044/133‑1 (MQ=255)
                                                           cTATCCAAATCCGCCGTTTCAACCTCCGCACTGGGGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCtt                                                                                      >  1:54511/1‑151 (MQ=255)
                                                               ccAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTg                                                                                    >  1:704561/1‑149 (MQ=255)
                                                                 aaaTCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgaga                                                                                 >  1:523890/1‑150 (MQ=255)
                                                                 aaaTCCGCCGTTTCAACCCCCGCACGGGTGATACCATCTCTGGAAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGaa                                                                                <  1:1423029/151‑1 (MQ=255)
                                                                      cgcctTTTCAACCCCCGCACTGTTAATACCATCTCTGGTAAGATACGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAAc                                                                               <  2:1218147/147‑1 (MQ=255)
                                                                        ccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAcc                                                                         <  1:1119664/151‑1 (MQ=255)
                                                                         cgTTTCACCCCCCGCACTGGTGATCCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAccc                                                                        <  1:981486/151‑1 (MQ=255)
                                                                            ttCACCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg                                                                      <  1:61518/150‑1 (MQ=255)
                                                                             tCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGc                                                                     <  1:957605/150‑1 (MQ=255)
                                                                              cAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTg                                                                   >  2:1375263/1‑151 (MQ=255)
                                                                                 cccccGCACTGGTGATCCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCt                                                                    <  2:633164/144‑1 (MQ=255)
                                                                                  cTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACg                                                               >  1:530802/1‑151 (MQ=255)
                                                                                       cagtGGTAATACCTGCTCTGGTAAGATTCGCCCGACGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa                                                            <  2:703411/146‑1 (MQ=255)
                                                                                        aCTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAct                                                         >  1:376487/1‑151 (MQ=255)
                                                                                        aCTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAct                                                         >  1:240644/1‑151 (MQ=255)
                                                                                            gTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg                                                     >  2:896765/1‑151 (MQ=255)
                                                                                              gATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGt                                                    <  1:464796/150‑1 (MQ=255)
                                                                                                tACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTg                                                 >  1:61935/1‑151 (MQ=255)
                                                                                                 aCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc                                                >  2:978087/1‑151 (MQ=255)
                                                                                                   cATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTg                                                 <  1:559731/148‑1 (MQ=255)
                                                                                                     tctcTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTAt                                            >  1:591268/1‑151 (MQ=255)
                                                                                                       tctGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATgg                                          >  1:1118052/1‑151 (MQ=255)
                                                                                                         tGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTa                                             <  2:1379957/146‑1 (MQ=255)
                                                                                                         tGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGa                                         >  1:1086857/1‑150 (MQ=255)
                                                                                                             aaGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg                                      <  2:791978/149‑1 (MQ=255)
                                                                                                             aaGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg                                    >  1:627009/1‑151 (MQ=255)
                                                                                                              aGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTgg                                   <  1:1232767/151‑1 (MQ=255)
                                                                                                               gATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGt                                  >  2:912508/1‑151 (MQ=255)
                                                                                                                 ttCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTa                                 >  2:88773/1‑150 (MQ=255)
                                                                                                                    gcccgccGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACgg                             >  1:987444/1‑151 (MQ=255)
                                                                                                                    gcccgccGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACgg                             >  2:871667/1‑151 (MQ=255)
                                                                                                                     cccgccGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACgg                             <  1:149689/150‑1 (MQ=255)
                                                                                                                     cccgccGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACgg                             <  1:912962/150‑1 (MQ=255)
                                                                                                                      ccgccgAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGtt                           <  1:683297/151‑1 (MQ=255)
                                                                                                                       cgccgAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTc                          <  1:645107/151‑1 (MQ=255)
                                                                                                                       cgccgAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTc                          <  1:93358/151‑1 (MQ=255)
                                                                                                                       cgccgAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTc                          >  2:394283/1‑151 (MQ=255)
                                                                                                                        gccgAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCt                         >  2:247125/1‑151 (MQ=255)
                                                                                                                           gAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACt                      >  1:986333/1‑151 (MQ=255)
                                                                                                                               gaagGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGa                    >  1:785894/1‑149 (MQ=255)
                                                                                                                                aagGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGa                    >  2:1181293/1‑148 (MQ=255)
                                                                                                                                 agGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGa                    >  1:628722/1‑147 (MQ=255)
                                                                                                                                    tGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGAttt              >  2:383775/1‑150 (MQ=255)
                                                                                                                                     gAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTaa            <  1:1057243/151‑1 (MQ=255)
                                                                                                                                      aaCGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCTTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAAc           <  2:735859/151‑1 (MQ=255)
                                                                                                                                      aaCGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAAc           <  1:686325/151‑1 (MQ=255)
                                                                                                                                        cGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACt          >  1:369082/1‑150 (MQ=255)
                                                                                                                                            aTTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCt       <  2:1272552/149‑1 (MQ=255)
                                                                                                                                            aTTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTcg     <  2:215801/151‑1 (MQ=255)
                                                                                                                                            aTTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTcg     >  1:115692/1‑151 (MQ=255)
                                                                                                                                            aTTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTc      <  1:988729/150‑1 (MQ=255)
                                                                                                                                              tttGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTcgc    <  1:1426308/150‑1 (MQ=255)
                                                                                                                                               ttGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGt  >  1:1296808/1‑151 (MQ=255)
                                                                                                                                                  |                                                                                                                                                   
TTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGT  >  NC_000913/3966605‑3966898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: