Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,097,5810AG41.7% 114.7 / 19.3 72noncoding (72/88 nt)serXtRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base A (6/36);  new base G (30/0);  total (36/36)
Fisher's exact test for biased strand distribution p-value = 2.37e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAA  >  NC_000913/1097436‑1097730
                                                                                                                                                  |                                                                                                                                                              
cACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑g                                                                                                                                                           <  2:254690/150‑1 (MQ=31)
 aCTGCTTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTc                                                                                                                                                             <  1:705726/148‑1 (MQ=11)
   tGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGAAGGGGGCTGCGCACCCCCCGGGTGTGTGTCATATACGAAAAAAAGGCCCGTAGTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑Gaa                                                                                                                                                         <  1:148182/149‑1 (MQ=11)
    gCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑cc                                                                                                                                                      <  2:1139336/150‑1 (MQ=33)
      gTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑ccccc                                                                                                                                                   <  2:1102048/151‑1 (MQ=35)
          aCGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATa                                                                                                                                               <  1:582764/151‑1 (MQ=39)
          aCGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGG‑GAGGGGGGGGTTCGGAA‑ccccc                                                                                                                                                   >  2:314081/1‑148 (MQ=255)
            ggTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGA‑GAGGGGGGGGGTT‑GCA‑ccccccgccc                                                                                                                                              >  1:450690/1‑145 (MQ=1)
                 gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTT‑‑GAA‑ccccccccttc                                                                                                                                             >  1:1163670/1‑139 (MQ=2)
                         ggCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATaca                                                                                                                                <  2:836823/150‑1 (MQ=255)
                                 ccAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCa                                                                                                                     <  2:1164016/151‑1 (MQ=255)
                                     ttGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTT‑GAA‑CCCCCCCTA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCg                                                                                                                 >  2:400762/1‑151 (MQ=255)
                                     ttGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGc                                                                                                                  <  1:323397/150‑1 (MQ=255)
                                       gTCGAACCCCGCTCGGGGCTTCTCATCCCCCCGGTGTCTACAATATACGAAAAAAAAGCCCTTACTTTCGTACGATCTCTTCTTTAAATATGCCGTT‑TATTGTCGCATTC‑GAA‑CCCCCTATA‑CTTTTCCGTATACA‑CAC‑TTTCCattctt                                                                                                                <  1:382926/150‑6 (MQ=14)
                                          gAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGGTT‑GAA‑CCCCCCATA‑CGTTTCCGTATACA‑CAC‑TTTCCAGGCGTg                                                                                                               >  2:722790/1‑148 (MQ=255)
                                                 ggTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGGTT‑GAA‑CCCCCCCTACCGTTGCC‑TGTTCA‑CAC‑TTTTCCAGCGTGCTCCCTccac                                                                                                     >  2:22224/1‑148 (MQ=11)
                                                    cGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGGAGGGGGGGGTT‑‑GCA‑CCCCCCCTA‑CGTTTCCGTGTATA‑CAC‑TTTCCAGGCgggggcccttagcccc                                                                                                  >  1:1165963/1‑136 (MQ=14)
                                                       ggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTc                                                                                                <  2:738982/150‑1 (MQ=255)
                                                       ggCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCg                                                                                               <  2:450819/151‑1 (MQ=255)
                                                          ttCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGG‑GGGGGGGGGGGTC‑GTA‑CCCCCCATA‑CCTTGTCGTATACC‑CAC‑CTTCCCGGCGTGCCCCTTCAGCCAcac                                                                                                >  1:1065632/1‑145 (MQ=17)
                                                                 ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTT‑GAA‑CCCCCCCTA‑CGTTGCCGTATACA‑CAC‑CTTTCCGGCGTGCTCCTTCAGCCCCCCGGAC‑A‑cccccc                                                                                   >  2:637982/1‑147 (MQ=17)
                                                                 ccccccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCa                                                                                    <  2:394318/150‑1 (MQ=255)
                                                                     ccGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGA‑GAGGGGGGGGTTC‑TAA‑CCCCCCCTA‑CGTTTCCCTATATA‑CAC‑CTTTCCGGCGGGCTCCTTCTGCCCCACCGAC‑A‑ccccccc                                                                                  >  1:150682/1‑143 (MQ=14)
                                                                      cGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGG‑GAGGGGGGGGTTT‑GAACCCCCCAAAA‑CGTTGCCG‑ATACA‑CACATTTCCCGG‑GTGCCCCTTCCTACCCCCGGGC‑A‑CCCCACCaaa                                                                               >  2:81295/1‑150 (MQ=9)
                                                                       ggTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAAtt                                                                             <  2:436849/151‑1 (MQ=255)
                                                                         tgtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTC‑GGA‑ACCCCGATA‑CGGTGGCGTATAAA‑AAC‑TTTTCAGGCGTGCTCCCTTCGCCACCCCGGC‑A‑CCTCCCCCAAtttt                                                                           >  2:650204/1‑149 (MQ=14)
                                                                          gtgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTc                                                                          <  1:714136/151‑1 (MQ=255)
                                                                           tgtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGA‑GGGGGGGGGGGTC‑GAA‑CCCCCCCTA‑CGTTGTCGTGTTAA‑CAC‑TTTCCACGCGCGCCCCTTCCACCACCCCGAC‑A‑cccccaccaa                                                                               >  1:410289/1‑137 (MQ=9)
                                                                           tgtgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GCGGGGGGGGTTT‑GCC‑TCCCCCGTC‑CCTTGCTCGATACA‑CAC‑CTTCCCCGCGTGGTCCCTTCCCCCCCCGGGC‑A‑CCCCACCACAtttt                                                                           >  1:520677/1‑147 (MQ=2)
                                                                             tgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGA‑GGGGGGGGGATTC‑GAA‑ACCCCCATA‑CGTTTCCCTCTAAA‑CAC‑CTTTCAGGCGGGCTCCTTCCACCCCCCCGAC‑A‑ccccccccacctttt                                                                          >  1:150052/1‑135 (MQ=9)
                                                                                aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTT‑GAA‑CCCCCCATA‑CCTTTCCCTATACA‑AAC‑CTTCCAGGCGTGCTCCTTCACCCCCCCGGGC‑A‑CCCCCCCCAAATTTT‑GTTCCt                                                                   >  2:45303/1‑151 (MQ=14)
                                                                                aaTATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCt                                                                   <  1:470696/151‑1 (MQ=255)
                                                                                    taCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTT‑GAA‑ACCCCCATA‑CGTTGGCGTATACA‑AAC‑TTTCCAGGCGGGCTCCTTCAGCCCCCCGGAC‑A‑CCCCCCCCAATTTTC‑GTTCCTGt                                                                 >  2:955264/1‑149 (MQ=14)
                                                                                     aCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTC‑GCA‑CCCCCGATA‑CGTTTCCGTATACA‑CAC‑CTTTCCGGCGTGCGCCCTCTGCCACTCCGGC‑A‑CCTCCCCCAATTGTT‑GTTTCCGTCtt                                                              >  1:726271/1‑151 (MQ=11)
                                                                                      cGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTT‑GAA‑ACCCCCCTA‑CGTTGCCCTATAAA‑CAC‑CTTCCCGGCGGGCTCCTTCCGCCCCTCCGAC‑A‑CCCCACCCAATTTTC‑GTTCCTGTCtt                                                              >  2:128632/1‑150 (MQ=14)
                                                                                      cGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCtt                                                              <  1:515903/150‑1 (MQ=255)
                                                                                       gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTC‑GAA‑CCCCCCATA‑CGTTTGCGTATAAA‑AAC‑TTTCCCGGGGTGGTCCTTCCCCCCCCCCGAC‑A‑CCCCCCCAAAATTTT‑GTTCCTTTCtt                                                              >  1:560471/1‑149 (MQ=2)
                                                                                       gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGc                                                            <  1:540605/151‑1 (MQ=255)
                                                                                       gAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGA‑GAGGGGGGGGTTT‑CAA‑CCCCCCCTT‑CGTTTCCGTTTAAA‑AAC‑TTTTCCGGCGGGGGCCTTCCGCCCCCCCGAC‑A‑CCCCCCCAAAATTTC‑GTTCCTCTCtt                                                              >  1:385318/1‑149 (MQ=9)
                                                                                        aaaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCt                                                           <  1:99404/151‑1 (MQ=255)
                                                                                         aaaaaaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTg                                                          <  2:154958/151‑1 (MQ=255)
                                                                                             aaaGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAAc                                                      <  2:271525/151‑1 (MQ=255)
                                                                                                gCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTT‑GCA‑CCCCCCCTA‑AGTTTTCGTATCAA‑CAC‑CTTCCAGGCGGGCTCCTTCAGCCCCCCGGCA‑A‑CCTCCCCACATTGTC‑GTTCCTTTCTTTCTTGGACggg                                                   >  1:1132845/1‑151 (MQ=9)
                                                                                                 cccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTC‑GAA‑ACCCCCCTA‑CCTTTTCGTATACA‑CAC‑CTTTCACGCGTGCTCCTTCAGCCACCCGGAC‑A‑CCCCCCCCAAATTTC‑GTTTCTGTCTTTCTGGGACggg                                                   >  2:491653/1‑150 (MQ=14)
                                                                                                  ccGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGgcg                                                 <  2:992964/151‑1 (MQ=255)
                                                                                                    gTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGG‑GAGGGGGGGGTTC‑GAA‑CCCCCCCTA‑CGTTTTCCTATACA‑CAC‑TTTTCAGGCGGGGTCCTTCCGCCACCCCGAC‑A‑CCCCCCCAAAATTTC‑GTTCCTGTCTTTCTGGGAAggg                                                   >  1:273200/1‑147 (MQ=9)
                                                                                                      aCTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTT‑GGA‑ACCCCCATA‑CGCTGCCCTATAAA‑CAC‑TTTTCAGGCGGGCTCCCTCAGCCACCCCGAC‑A‑CCCCACCCAATTCTT‑GTTTCTGTCTTGCTTGAACCGGCGCTa                                              >  2:634381/1‑150 (MQ=9)
                                                                                                      aCTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGTTT‑GAA‑ACCCCCCTA‑CGTTTCCGTATTCACCCC‑TTTCCAGGGTGGTTCCTTC‑TCCCCTCCGGC‑A‑CCCCCCCAAATTTTT‑GTTCCTGTTTTTCTGGGACGGgggc                                                >  1:138201/1‑148 (MQ=2)
                                                                                                       cTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTa‑a                                            <  2:126114/150‑1 (MQ=255)
                                                                                                        tttCGTACGAGCTCTTCTTTAAATATGGCGGT‑GGGGGGGGGGGTT‑GAA‑ACCCCCACA‑CGTTGTCGTATAAA‑CAC‑CTTTCCGGCGGGGTCCCTCTGCCACCCGGGCAA‑CCCCCCCAAATT‑TT‑GTTTCTGTCTTTTTGGGACAGGCGCCA‑Att                                          >  1:712119/1‑151 (MQ=2)
                                                                                                         ttCGTACGAGCTCTTCTTTAAATATGGCGGT‑TGGGGGGGGGTTT‑GAA‑CCCCCCCTA‑CGTTGCCGTATAAA‑CAC‑CTTTCCGGGGTGGTCCCTCAGCCCCCCCGGC‑ACCCCCCCCAAATTTTC‑‑TTTCTTTCTTTCTTGGAAGGGCGCCA‑Attt                                         >  1:598421/1‑151 (MQ=2)
                                                                                                           cGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGGTG‑GAA‑CACCCCCTC‑CATTGCCGTATATC‑CAC‑CTTTCTGGGGGGGTCCTTCAGACACACGGGC‑A‑CCCCCCCCAAATTTC‑GCTCCTGTTTTGTTTGAAAGGgggcccatt                                           >  1:315653/1‑143 (MQ=2)
                                                                                                           cGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAg                                       <  1:314012/151‑1 (MQ=255)
                                                                                                             tACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGGGTT‑GAA‑ACCCCCCTA‑CGTTGCCGTATACA‑CCC‑TTTTCAGGCGTGGTCCTTCATCCACCCGGGC‑A‑CCTCACCAAAATTTC‑GTTCCTGTCTTGCTTGAACAGGCGCTA‑Atttttg                                      >  2:733544/1‑147 (MQ=17)
                                                                                                                gAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGaaa                                  <  1:1071409/151‑1 (MQ=255)
                                                                                                                  gCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATc                                <  2:921387/151‑1 (MQ=255)
                                                                                                                       tcttTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCAt                              <  2:726474/148‑1 (MQ=255)
                                                                                                                       tcttTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGa                            <  1:618179/150‑1 (MQ=255)
                                                                                                                        cttTAAATATGGCGGT‑GAGGGGGGGGTTC‑GAA‑CCCCCCCTA‑CCTTTCCGTATACA‑CAC‑TTTCCACGCGTGCTCCTTCAGCCCCCCGGAC‑A‑CCCCCCCCAATTTTC‑GTTCCTGTCTTGCTGGGACGGGCGCCA‑ATTTTTGGGAATCATGAcc                          >  2:831726/1‑151 (MQ=17)
                                                                                                                        cttTAAATATGGCGGT‑GAGGGGGGGAATC‑GAA‑CCCCCGGAA‑CGTTTTCGTATACA‑CAC‑TTTTCAGGCGGGGTCCCTCAGCCACCCCGGC‑A‑CCCCACCAAATTTTCGGTTCCT‑TCTTGCTGGGAAGGGGGCCACTTTTAGGGaaacaagacc                           >  1:711087/1‑144 (MQ=2)
                                                                                                                           tAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTg                        <  2:659804/150‑1 (MQ=255)
                                                                                                                           tAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TATCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTg                        <  2:481009/150‑1 (MQ=255)
                                                                                                                              atatGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTGAGGt                    >  2:149384/1‑151 (MQ=255)
                                                                                                                              atatGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTGAGGt                    <  1:413714/151‑1 (MQ=255)
                                                                                                                                  ggCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCACGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTGAGGTcaac                >  1:169610/1‑151 (MQ=255)
                                                                                                                                   gCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTGAGGTcaaaa               <  2:356940/151‑3 (MQ=255)
                                                                                                                                      gT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTGAGGTCAACAAAc            >  1:186930/1‑151 (MQ=255)
                                                                                                                                         gAGGGGGGGATTC‑GAA‑CCCCCGATG‑CGTTGCCGTATACG‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCGCCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTGAGGTCAACAAACt           <  1:661648/150‑1 (MQ=255)
                                                                                                                                         gAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCCCCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTGAGGTAAACAAACtt          >  1:434708/1‑151 (MQ=255)
                                                                                                                                             gggggATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTg      <  2:803434/151‑1 (MQ=255)
                                                                                                                                             gggggATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTg      <  2:564470/151‑1 (MQ=255)
                                                                                                                                                 gATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGaaaa  <  2:1101324/151‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                              
CACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGT‑GAGGGGGGGATTC‑GAA‑CCCCCGATA‑CGTTGCCGTATACA‑CAC‑TTTCCAGGCGTGCTCCTTCAGCCACTCGGAC‑A‑CCTCACCAAATTGTC‑GTTCCTGTCTTGCTGGAACGGGCGCTA‑ATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAA  >  NC_000913/1097436‑1097730

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑