Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,376,5050AC44.9% 117.3 / 26.8 69T158P (ACC→CCC) ycjRputative TIM alpha/beta barrel enzyme
Reads supporting (aligned to +/- strand):  ref base A (37/1);  new base C (0/31);  total (37/32)
Fisher's exact test for biased strand distribution p-value = 8.04e-19
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATT  >  NC_000913/1376356‑1376651
                                                                                                                                                        |                                                                                                                                                  
gCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAacac                                                                                                                                                   >  1:434960/1‑151 (MQ=255)
gCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAaca                                                                                                                                                    <  2:110672/150‑1 (MQ=255)
   tACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACAccc                                                                                                                                                 <  1:945100/150‑1 (MQ=255)
         cgATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCg                                                                                                                                           >  1:581572/1‑150 (MQ=255)
                      gcgTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTAc                                                                                                                             >  2:474402/1‑151 (MQ=255)
                         tAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAtc                                                                                                                          >  2:537659/1‑151 (MQ=255)
                           gCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGCACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGCTCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAtcg                                                                                                                         >  1:549650/1‑150 (MQ=255)
                            ccgggAGGGCGCCCGAAAAGGGGGGTGTGTTCCCCTGCGCGTCCGGGAACGGGCCGCCGCGCGTCCCGGACCGGTGTTGTTTCTCGAACCTTAAACCCCCTTTAGGAATCATATGCACACCCCCCTCGCCGATGCCCGCCGTTa                                                                                                                              <  1:761344/141‑1 (MQ=255)
                             cTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACAtcgtcg                                                                                                                      >  2:530164/1‑151 (MQ=255)
                                      gcccaaaaaggggTGAGTCTTTCCCAGCGCGTGCGGAAAAATTTCCCCGCGGGACCGGGAAACGTGGTGTACCAAAAACCTTAAAACCAATTGCAGGACAATAAGCACAACCCCCCCGCCAAGGCCCGCCGTTACATCGTCGAAAACg                                                                                                                <  2:997940/137‑1 (MQ=255)
                                        cggaaagaTGGTGAGTGTTCCCCGGCGCGTAGTGGAACGGGTCGCCGCGCGGACCGAACCGGTGGTTTCTCTCGAACCTTAAACCCGCTATCAGGTTCATATGACAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCtt                                                                                                           <  1:197332/144‑1 (MQ=255)
                                         cGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCtt                                                                                                           >  1:450692/1‑150 (MQ=255)
                                          gCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTa                                                                                                          >  2:303528/1‑150 (MQ=255)
                                           cAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTaa                                                                                                         >  2:1187459/1‑150 (MQ=255)
                                            aaaaTGGTGAGTGTTTCCCTGCGCGTACGGGAACAGGTCGCCGCGCGTCCCGAACCCGGGGTGTATCTCGACCCTTAAACCCGCTATAAGGATAATATAATAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTa                                                                                                          <  2:978956/148‑1 (MQ=255)
                                                   tgagtgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                                 >  2:151958/1‑150 (MQ=255)
                                                    gagtgtTCCCCGGCGCGTCCGGGAACAGGTCGCCGCGCGTCCCGGACCCGTGGTTTATCGCAACCCTTAAACCCGCTATAAGGATCATATGATAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACa                                                                                                <  2:54374/150‑1 (MQ=255)
                                                    gagtgaTTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTAc                                                                                                 >  2:294583/1‑149 (MQ=255)
                                                      gtgttccccGGCGCGTACGGAAACAGGTCGCCGCGCGTCCGGGACCGGGGGTGTATCTCGACCCGTAAACCCGCTAACAGAATCATATGATCAACCCCCCCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAg                                                                                               <  2:1106886/143‑1 (MQ=255)
                                                           tCCCGGCGCTTACTGAAACAGGCCGCGGGGCGAGCCGGAACGGTGTTGTACCTCGACCCGTTAACCGGCTAACAGTATCAGATAACGACCCCCCGCGGCGTTGGCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATc                                                                                         <  1:483141/150‑1 (MQ=255)
                                                           ccccGGCGCGTAGGGAAACAGCTCCCCGCGCGCCCCGGACCCGTGTTGTATCTCGACCTTTTAACCCCCTATCGGTAAAATAGAATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATc                                                                                         <  1:676134/149‑1 (MQ=255)
                                                              cTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCg                                                                                     >  1:549327/1‑151 (MQ=255)
                                                               tGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCg                                                                                     >  2:155731/1‑150 (MQ=255)
                                                                      acgggAACAGGTCGCCGCGCGTCCCGGAACGGTGGTTTATCCCGACCCTTAAACCCGCTACCAGAATCATATAAACAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGAttt                                                                                 <  2:493747/144‑1 (MQ=255)
                                                                      aCTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTAt                                                                             >  2:989009/1‑151 (MQ=255)
                                                                         ggAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcac                                                                          >  2:769529/1‑151 (MQ=255)
                                                                           aaCAGGTCGCCGCGCGTACCGGACCGGGGGTGTATCTCAACCCTTTAACCCGCTATCAGGATCATATGATAACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATcaca                                                                         <  2:365641/150‑1 (MQ=255)
                                                                               ggTCGCCGCGCGTACCGAACCGGGGGTGTCCCCCGACCCTTAAACCCGCTATAGGGATAAAATAACCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTa                                                                              <  1:1189297/141‑1 (MQ=255)
                                                                                  cgccgcGGCTACGGGATCTGTGTTGTATCTCGAACCTTTAAACCTCTACCAGGATCATATGACCACCCCCCCCCACGCTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATg                                                                       <  1:460469/145‑1 (MQ=255)
                                                                                    ccgcGCGTCCCGGACCCGTGGTGTATCTCGAACCTTTAACCCGCTACCAGGATAATATGATCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                                <  2:114220/150‑1 (MQ=255)
                                                                                    ccgcGCGTCCCGGACCCGTGGTGTATCTCGAACCGTTAACCCGCTATAAGGATCATATGATAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCg                                                                <  2:542291/150‑1 (MQ=255)
                                                                                    ccgcGCGTACCGGACCGGTGGTTTACCCCAAACCGTTAACCCGCTATCAGTATCTTATGACCACCCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAata                                                                   <  2:383219/147‑1 (MQ=255)
                                                                                    ccgcGCGTACGGAACCGGTGGTTTATCTCGAACCGTTAACCCGCTATAAGAATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAatat                                                                  <  2:1009062/148‑1 (MQ=255)
                                                                                    ccgcGCGCCCCGAACCGGTGGTTTATCCCAACCCGTTAACCCGCTAACAGAATAATATAAAAAACCCCCCCGCGGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAata                                                                   <  1:1124697/147‑1 (MQ=255)
                                                                                       cgcgTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCga                                                               >  2:460525/1‑148 (MQ=255)
                                                                                        gcgTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                            >  2:207478/1‑150 (MQ=255)
                                                                                         cgTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaag                                                             >  1:404641/1‑148 (MQ=255)
                                                                                          gtcccGGAACGGTGGTGTTTCTCGACCCTTTAACCCGCTACAAGGATCAAATGATCACCCCCCGCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAagaaga                                                         <  2:743628/148‑1 (MQ=255)
                                                                                          gTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                            >  1:889824/1‑148 (MQ=255)
                                                                                          gTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaaga                                                            >  1:926004/1‑148 (MQ=255)
                                                                                            aCCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGAt                                                        >  2:29274/1‑150 (MQ=255)
                                                                                            aCCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGAt                                                        >  2:1066802/1‑150 (MQ=255)
                                                                                               ggAACCGTGGTGTCCCCCAACCCGTAAACCCGTTATCGGAATAATATGACAACCCCCCCCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATaa                                                      <  2:1003583/149‑1 (MQ=255)
                                                                                               gaaaCGGTGGTGTATCTAGACCCGTTAACCCGTTATCAGGATCATAGGAAAAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATaa                                                      <  2:405465/147‑1 (MQ=255)
                                                                                                ggaccggtggttTATCTCGACCCGTAAACCCGCTATAAGTATCATATGATAACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATa                                                       <  2:785423/142‑1 (MQ=255)
                                                                                                 aaCCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTg                                                  >  2:355614/1‑151 (MQ=255)
                                                                                                 aaCCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTg                                                  >  2:1104395/1‑151 (MQ=255)
                                                                                                  accggtggttTATCCCGACCCTTAAACCCGCTATCAGGATCATATGATCAACCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCgaagaa                                                           <  2:1012290/138‑1 (MQ=255)
                                                                                                  aCCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTg                                                  >  1:951600/1‑150 (MQ=255)
                                                                                                   ccGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTgg                                                 >  2:685299/1‑150 (MQ=255)
                                                                                                    cGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgcg                                               >  2:525635/1‑151 (MQ=255)
                                                                                                      gtgttttacccctagccGTAAACCCGCTAAAAGTATCATATGACAACCCCCCCCGCCGTTGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGgcgc                                              <  1:427180/136‑1 (MQ=255)
                                                                                                          tgtATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgc                                          >  2:925664/1‑151 (MQ=255)
                                                                                                           ggaTCTCGAACCGTTAAAACGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGgc                                          >  1:1142425/3‑150 (MQ=255)
                                                                                                               ctcGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGc                                     >  1:290830/1‑151 (MQ=255)
                                                                                                                 cGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCAt                                   >  2:823291/1‑151 (MQ=255)
                                                                                                                   acccGTAAACCCGTTATCAGGATCATATGACCAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTg                                      <  2:886762/144‑1 (MQ=255)
                                                                                                                          aaaCTGCTATCAGGATCATATGACAAACCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACaa                              <  2:1071937/147‑1 (MQ=255)
                                                                                                                           aacccGTTATCAGAATAATATGATCACCCCCCCCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcg                           <  2:450821/147‑1 (MQ=255)
                                                                                                                           aacccGCTATCAGTATAATATAACCACCCCCCCCGCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgc                          <  2:695159/148‑1 (MQ=255)
                                                                                                                           aaCCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACcgcg                         >  1:783461/1‑151 (MQ=255)
                                                                                                                              ccGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGAc                       >  1:903380/1‑151 (MQ=255)
                                                                                                                                gCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCt                     >  2:1027669/1‑151 (MQ=255)
                                                                                                                                     acagggccagaggATAAACCCCCGCCCCGAGGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTc                 <  1:50581/139‑1 (MQ=255)
                                                                                                                                       ggATAATATGACCCACCCCCCCCCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGc                   <  1:135065/146‑1 (MQ=255)
                                                                                                                                       agaaTCATATGATCACCCCCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCgg               <  2:797224/148‑1 (MQ=255)
                                                                                                                                             atatGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgt         >  2:108479/1‑151 (MQ=255)
                                                                                                                                               atGATCACCCCCCCCGCCGATCCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCAtgtg        <  2:515773/150‑1 (MQ=255)
                                                                                                                                                   tCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCatat   >  2:401554/1‑151 (MQ=255)
                                                                                                                                                    cAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAtt  >  2:584522/1‑151 (MQ=255)
                                                                                                                                                    cAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATAtt  >  2:1028419/1‑151 (MQ=255)
                                                                                                                                                        |                                                                                                                                                  
GCTTACCGCCGATGACCTCGCCGCGTAGCCTGGACGGCGACCGCAAAATGGTGAGTGATTCCCTGCGCGTACTGGAACAGGTCGCCGCGCGTACCGGAACCGTGGTGTATCTCGAACCGTTAAACCGCTATCAGGATCATATGATCAACACCCTCGCCGATGCCCGCCGTTACATCGTCGAAAACGATCTTAAACATGTACAGATTATCGGCGATTTCTATCACATGAATATCGAAGAAGATAACCTGGCGCAGGCGCTGCATGACAACCGCGACCTGCTCGGTCATGTGCATATT  >  NC_000913/1376356‑1376651

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: