Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,252,646	0	T	G	42.0%	132.7 / 11.8	69	V316G (GTA→GGA)	yeiI	putative kinase
Reads supporting (aligned to +/- strand):  ref base T (6/34);  new base G (29/0);  total (35/34)
Fisher's exact test for biased strand distribution p-value = 8.02e-14
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATG  >  NC_000913/2252498‑2252793                                                                                                                                                     |                                                                                                                                                        cAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGta                                                                                                                                                        <  1:249864/150‑1 (MQ=255) cAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGCCCTGGtg                                                                                                                                                        >  1:1154554/1‑149 (MQ=255)   gCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGtatat                                                                                                                                                     <  1:1017683/151‑1 (MQ=255)    cAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGtatata                                                                                                                                                    <  2:1092125/151‑1 (MQ=255)      aGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGc                                                                                                                                                  <  1:1067872/151‑1 (MQ=255)          gtTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCttt                                                                                                                                               <  1:762218/150‑1 (MQ=255)          gtTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑c                                                                                                                                             <  1:46301/151‑1 (MQ=255)          gtTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGCtttt                                                                                                                                              >  1:295504/1‑150 (MQ=255)            tCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑Ct                                                                                                                                            <  2:569054/150‑1 (MQ=255)               gCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGTTATAGGttt‑tttt                                                                                                                                          >  2:783520/1‑148 (MQ=255)                cAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGTTTATAACttt‑ttt                                                                                                                                           >  2:841943/1‑147 (MQ=255)                cAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGCTATTGATTT‑Ctt                                                                                                                                           >  2:378124/1‑147 (MQ=255)                cAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAg                                                                                                                                       <  2:765102/151‑1 (MQ=255)                 aaCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCttt‑ttttgag                                                                                                                                       >  2:840626/1‑146 (MQ=255)                 aaCTGGTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGACTGGGATATATTTTT‑Cttttt                                                                                                                                        >  1:433643/1‑146 (MQ=255)                  aCTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGgatataatttttttttagg                                                                                                                                       >  2:1094412/1‑137 (MQ=255)                   cTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGtattt                                                                                                                                                     >  2:354596/1‑133 (MQ=255)                    tgtttgttTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGGTATAGGTTT‑TTTTAAgg                                                                                                                                      >  2:33237/1‑148 (MQ=255)                    tgtttgttTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATAGCttt‑ttt                                                                                                                                           >  1:612579/1‑143 (MQ=255)                     gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAGGGATGGAGAACATTTTTTGCTGACCGCGCAAGCGCATACGACAGAAGAAGGTTTTGGGGTTGACGAGGGTTTTATGCGGGGCCGGGTAAATAGTttt‑tttttagg                                                                                                                                      >  1:1011500/1‑142 (MQ=255)                     gtttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATa                                                                                                                                   <  2:425493/150‑1 (MQ=255)                       ttgtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTTGGCTATAgaattttt                                                                                                                                            >  2:889079/1‑133 (MQ=255)                         gtttATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGCCCTGGGTTATAGCCTT‑TTTTAAGGATACAggg                                                                                                                              >  2:523394/1‑149 (MQ=255)                             aTTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTcc                                                                                                                           <  2:128625/150‑1 (MQ=255)                             aTTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTcc                                                                                                                           <  2:387875/150‑1 (MQ=255)                               ttGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCcgc                                                                                                                         <  2:477728/150‑1 (MQ=255)                                 gCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGa                                                                                                                       <  2:84402/150‑1 (MQ=255)                                   ccGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTAGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACa                                                                                                                     <  1:1023729/150‑1 (MQ=255)                                     gATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAgcg                                                                                                                  <  1:33903/151‑1 (MQ=255)                                     gATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGATTT‑CTTTAAGGGTaaacgtttc                                                                                                                           >  1:1193000/1‑134 (MQ=255)                                            cAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGttt                                                                                                           <  1:246086/151‑1 (MQ=255)                                            cAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGttt                                                                                                           <  1:31535/151‑1 (MQ=255)                                            cAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGttt                                                                                                           <  1:665326/151‑1 (MQ=255)                                             aGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGGTATAGATTT‑TTTTTAGGGTAAAGTTTTCGCGCAAGCGcccccttt                                                                                                          >  1:531623/1‑146 (MQ=255)                                                  aTTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTACGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGt                                                                                                     <  2:154370/151‑1 (MQ=255)                                                    tGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGCTTT‑TTTTTAGGATACAGTTTTCGCGACAGCGCCCGtttt                                                                                                          >  2:564559/1‑144 (MQ=255)                                                           aaaaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCGGGGGTATTGCTTT‑TTTTTAGGGTACAGTTTTCCCGGCAGCGCCCGcttttccgtgggc                                                                                                 >  2:638789/1‑137 (MQ=255)                                                            aaaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTTGGGTAATGGTTT‑CTTTTAGGAGAAAGTTTCCCCGCCAGCCCCCGCTTTTCGGTGGCCTCcgcg                                                                                           >  2:459172/1‑151 (MQ=255)                                                            aaaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCGCGGGGTATAGCCTT‑TTTAGAGGATACAAGTTGCGCGACCAGGCCCGGTTTGCGGTGGCCcgcgcg                                                                                           >  1:956892/1‑151 (MQ=255)                                                             aaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGTTTTAACTTT‑TTTTTAGGAGACAGTTTTCGCGCCCGCGCCCCTTTTTCGGTTGcc                                                                                                 >  1:384859/1‑144 (MQ=255)                                                             aaGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGCTTT‑TTTTTAGGGAACAGGTTTCGCGACAGCCCCCCCTTTTTGGGAGCCTGCGCgg                                                                                          >  2:983521/1‑151 (MQ=255)                                                                gATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCgg                                                                                          <  1:11024/148‑1 (MQ=255)                                                                   ggAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAAttt                                                                                    <  2:154584/151‑1 (MQ=255)                                                                     agaACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGATAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTcg                                                                                  <  2:1070272/151‑1 (MQ=255)                                                                        aCAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTGGGATATATCTTT‑TTTGTAGGGTACAGGTTTTGCGGCCGCGCCCGCTTTTCGGGAGCCTGCGCCGGCATTTtgcg                                                                                >  1:153336/1‑150 (MQ=255)                                                                         cAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGGTATAGCTTT‑TTTTTAGGGTACAATTTTCCCGACAGCGCCCGCTTTTCGGGAGCCTGCGCGGCAAtttttcgc                                                                               >  2:326397/1‑145 (MQ=255)                                                                         cAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAAGTTT‑TTTTTAGGGTACAGTTTTCCCGGCAGCGCCCGCTTTTCGGTTGCCTGCGCGGCAAttt                                                                                    >  1:994525/1‑145 (MQ=255)                                                                          aaTTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATATAGATTT‑TTTTAAGGGTACAGGTTTCGCGGCAGCGCCCGTTTTTTGGGTGCCTGCGCGGCAATTTtgcgcg                                                                              >  2:644665/1‑150 (MQ=255)                                                                            tttttGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAACTTT‑TTTTTAGGATAAAGTTTCCGCGACAGCGCCCGGTTTTCGGTAGCCTGCGCGGCCATTTTTCGCGcc                                                                            >  2:942179/1‑150 (MQ=255)                                                                                 gCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGc                                                                      <  2:1136867/151‑1 (MQ=255)                                                                                   tGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGCTTT‑CTTTTAGGATAAAGATTTCGCGGAAACGCCCCTTTTTTGGTGGCCTCCGCGGCGATTTTTCTCGCCacccg                                                                       >  1:926816/1‑144 (MQ=255)                                                                                    gACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCa                                                                     <  2:808621/149‑1 (MQ=255)                                                                                    gACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGGATATAGCTTT‑TTTTTAGGATACAGTTTTCGCGACAGCGCCCCCTTTTCGGTAGCCCGCGCGGCAATTTTTCGCGcc                                                                            >  2:560325/1‑142 (MQ=255)                                                                                     aCCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCCGGATATAGACTT‑TTTTTAGGATAAAAATTTCGCGAAAACGCCCGCTTTGCGGGGGGCTGCGCGGCa                                                                                        >  1:876258/1‑129 (MQ=255)                                                                                        gcgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATAGATTT‑TTTTGAGGATAAAAATTTCGCGCGAGCGCCCCTTTTTCGGGAGACTGCGCGGCACATTTGCTCGcc                                                                            >  2:771712/1‑138 (MQ=255)                                                                                        gcgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTaa                                                            <  2:472306/151‑1 (MQ=255)                                                                                         cgcCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTaaa                                                           <  2:734940/151‑1 (MQ=255)                                                                                                  aTACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCCGGGATATAGCTTT‑TTTTTAGGGTAAAATTTTCGCCACAGCCCCCCGTTTTTGGTAACCCGCGCCGCCATTTCGCGCGCCCGCGGCC‑G‑C‑CTTAACAacccc                                                    >  2:464694/1‑148 (MQ=255)                                                                                                       aCAGTAGACAGTTTTGGTGCTGACTATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGt                                             <  2:362838/151‑1 (MQ=255)                                                                                                         aGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTTGTATATAGCTTT‑TTTTGAAGAAACAGTTTCCCCGACCGGGCCCCTTTTGTGGTTGCCCTCGCGGCAAGTTTGCGCGCCCGCGGCC‑G‑CTTTAAAAAaccctccccg                                               >  1:943610/1‑147 (MQ=255)                                                                                                         aGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCt                                           <  2:995456/151‑1 (MQ=255)                                                                                                         aGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCt                                           <  2:446949/151‑1 (MQ=255)                                                                                                           tAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCCGGGATTTAGGTTT‑TTTTTAGGGAAAAATTTTCCCGGCAACGCCCCTTTTTTGGGGGCCCGCGCGGCAAATTTTCGCGCCCGGGGCC‑GCC‑TTAAAAAacccaccc                                                 >  2:111276/1‑140 (MQ=255)                                                                                                            aGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGGCTTGGGTATAAGTTT‑TTTTTTGGGAACAGTTTCCCGGGCAACGCCCCCTTTACTGTAGCCTTCGCGGCGATTTCCCCCGCCCGCGGGG‑G‑C‑TTTAACCaacccaccc                                                >  2:997611/1‑143 (MQ=255)                                                                                                             gACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTGTATAGCTTT‑TTTTGAGGAAACAGTTTTCGCGTCAGCGCCCGTTTTGGGGTAGCCTGCGGGGCAATTTTGCCCGCCAGCGGCAGG‑C‑TCAAAAAaccccccctctcctcc                                         >  1:379466/1‑138 (MQ=255)                                                                                                             gACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCTGCCg                                       <  1:929176/151‑1 (MQ=255)                                                                                                                         gCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCttt                           <  2:690101/151‑1 (MQ=255)                                                                                                                         gCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCttt                           <  1:863614/151‑1 (MQ=255)                                                                                                                           tGACGATGGTTTTATGGCGGGCCGCGTGTATAATTTTCCTTTAAGGAACAAGTTTTC‑CGAGAGCGGCCCTTTTAGGGTGGGCTGCCGGGCAATTTTCCGCGCCAGCGGCG‑G‑C‑CTTAACAACCCCCCCCCCTCTGcccgtaaaccacgat                           >  1:1126842/1‑136 (MQ=255)                                                                                                                            gACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCa                         <  2:101395/150‑1 (MQ=255)                                                                                                                            gACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCAt                        <  1:644496/151‑1 (MQ=255)                                                                                                                                  ggTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGc                  <  2:723254/151‑1 (MQ=255)                                                                                                                                           gCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCAt                        <  2:710225/136‑1 (MQ=255)                                                                                                                                           gCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCAt                        >  1:710030/1‑136 (MQ=255)                                                                                                                                             gggCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTaa       <  2:615355/151‑1 (MQ=255)                                                                                                                                                     tatataGCTTG‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCTGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCTCTTTCATTAGTGCCTATGGTGTAACAATg  <  1:763189/148‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                                        CAGCAAGGTGTTCAGCAACTGTTTGTTTATTTGCCCGATGAGTCAGTTTATTGCAGCGAAAAGGATGGAGAACAATTTTTGCTGACCGCGCCAGCGCATACGACAGTAGACAGTTTTGGTGCTGACGATGGTTTTATGGCGGGCCTGGTATATAGCTTT‑CTTGAAGGATACAGTTTCCGCGACAGCGCCCGTTTTGCGGTAGCCTGCGCGGCAATTTCGCGCGCCAGCGGCA‑G‑C‑TTAAACAACCCTACCCTGTCTGCCGATAACGCGCTTTCATTAGTGCCAATGGTGTAACAATG  >  NC_000913/2252498‑2252793

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑