Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,921,173	0	T	C	46.2%	69.0 / 12.2	52	I310V (ATT→GTT)	gudP	putative D‑glucarate transporter
Reads supporting (aligned to +/- strand):  ref base T (25/3);  new base C (0/24);  total (25/27)
Fisher's exact test for biased strand distribution p-value = 6.92e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACT  >  NC_000913/2921027‑2921319                                                                                                                                                   |                                                                                                                                                   cAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATccca                                                                                                                                                <  1:119786/151‑1 (MQ=255)  aGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATcccac                                                                                                                                               <  2:14865/151‑1 (MQ=255)        aTAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGc                                                                                                                                          >  1:274650/1‑150 (MQ=255)        aTAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGc                                                                                                                                          >  2:691552/1‑150 (MQ=255)         tAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGca                                                                                                                                         >  2:364029/1‑150 (MQ=255)                gatgatCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATTGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACAccgccg                                                                                                                                 >  1:1157019/1‑151 (MQ=255)                            ctaaaCGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTCCGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAaaaccgc                                                                                                                       <  2:856102/146‑1 (MQ=255)                                    tGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcgg                                                                                                             >  1:705472/1‑151 (MQ=255)                                        gTAGTTGCACACCCCCACCACCAGCGACAGCAACCTGCCCATAACGATCGGTGTTTCCCGCGCAGTGTCCAGCGACCCCGTGCGGCGCACCAGCCAGTCGGAAATAACCCCACCCACCCCACCGCCGATAAAACCGCAAACCGCcggaa                                                                                                           <  1:779768/149‑1 (MQ=255)                                         tAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCcggaacgg                                                                                                        >  2:537258/1‑151 (MQ=255)                                            ttGAAGAACCCCATCCCCATCGACAGCAACATGCCCATTACGATGGGTTTTTTCCGCGCAATGTTCAGCGACCCCGTGCGGCGCATCAGCCAGTCGGAAATAACCCCCCCCAGCCCACCGCCGATAAAACCGCAAACCGCcggaacg                                                                                                         <  2:126445/147‑1 (MQ=255)                                                aGAACCCCACCCCCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGAAATGTTCAGCGACCCCGTGCGGCGCATCAGCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGc                                                                                                    <  2:862124/148‑1 (MQ=255)                                                     accatcaccatcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGc                                                                                            >  2:1052811/1‑151 (MQ=255)                                                       catcaccatcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGccc                                                                                          >  1:548115/1‑151 (MQ=255)                                                       cacccccatcGACAGCAACATGCCCATTACGATGGGTTTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGccc                                                                                          <  2:848016/146‑1 (MQ=255)                                                           accatcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCtt                                                                                      >  2:467223/1‑151 (MQ=255)                                                            ccaccGACACCAACTTCCCCTTTACGACGGGTTTTTTACGCGCATTTTCCAGCAACCCCGTGCGGCGCACAAGCCAGTCGGAAATAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCtt                                                                                      <  2:116812/150‑1 (MQ=255)                                                             catcGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTc                                                                                    >  1:214290/1‑151 (MQ=255)                                                              accGACAGCACCATGCCCATTACGATCGGGGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCTGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTc                                                                                    <  1:901661/148‑1 (MQ=255)                                                                       aaCATGCCCTTTACGGTCGGTGTTTTCCGCGAAAGGTCCAGCGACCCCGGGCGGCCCATCAGCCAGTCGAAAAAAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATc                                                                              <  1:378219/147‑1 (MQ=255)                                                                       aaCATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGAc                                                                           >  2:967898/1‑150 (MQ=255)                                                                          aTGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATcc                                                                       >  2:469070/1‑151 (MQ=255)                                                                             cccATTCCGAACGGGGTTTTACGCGCAATGTTCAGCGATCCCGAGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGGCTCGGCAGCCcg                                                                     >  1:866509/1‑150 (MQ=255)                                                                               cATTCCGATCGGTTTTTTCCGCCAAATTTCCAGCGATCCCGTGCGGCGCAACAGCCAGGCGAAAATACCCCCCCCCACCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCcgcg                                                                   <  1:1089509/150‑1 (MQ=255)                                                                                    cGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTg                                                             >  2:442099/1‑151 (MQ=255)                                                                                           tgtTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCAcc                                                         >  2:724038/1‑148 (MQ=255)                                                                                            gtTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCAcc                                                         >  2:308943/1‑147 (MQ=255)                                                                                             ttttCCGCGAAATTTCCAGCGACCCCGTGCGGCGCATCACCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGa                                                      <  2:257055/149‑1 (MQ=255)                                                                                             ttttCCGCGAAATGTTCACCGCCCCCGTGCGCCGCTCCAGCCAGTCGAAAATACCCCCCCCCAGCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGAt                                                     <  1:606933/150‑1 (MQ=255)                                                                                                  cgcgCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAAcc                                                >  1:182473/1‑150 (MQ=255)                                                                                                   gcgcAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCgg                                              >  1:23010/1‑151 (MQ=255)                                                                                                   gcgcAATGTCCAGCGCCCCCGTGGGGCGCCCCAGCAGGTGGAAAATAACCCCCCCCACCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAAc                                                 <  1:292513/148‑1 (MQ=255)                                                                                                    cgcAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCggg                                             >  1:57375/1‑151 (MQ=255)                                                                                                       aaTGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAAc                                          >  2:586863/1‑151 (MQ=255)                                                                                                         tGTCCAGCGACCCCGGGCGGCGCACCACCGAGTGGAAAATACCCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGa                                            <  2:848819/147‑1 (MQ=255)                                                                                                            tCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGt                                     >  2:174711/1‑151 (MQ=255)                                                                                                             caccgctCCCGTGCGGCGCATCAGCAAGCCGAAAAAACCCCCCCCAACCCCCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACAGCCCGCGTGCCTGCACCAGATAAACCGGGaa                                           <  1:961887/138‑1 (MQ=255)                                                                                                              aGCGATCCCGTGCGGCGCACCAGCCGTTCGAAAATAACCCCCCCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAgg                                      <  2:240408/148‑1 (MQ=255)                                                                                                                 gaccccGGGCGGGGCACGACCCAGCCGAAAAAACCCCCCCCCAGCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGtaataa                                <  2:1019764/148‑1 (MQ=255)                                                                                                                 gATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGtaata                                 >  2:858002/1‑150 (MQ=255)                                                                                                                  accccGGGCGGCCCACCAGCCAGTCGAAAATAACCCCCCCCAGCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGtaataa                                <  2:590977/148‑1 (MQ=255)                                                                                                                    cccggcgggcgCATCAGCCAGTCGGAAAAAACCCCCCCCAGCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAaaga                             <  2:292776/144‑1 (MQ=255)                                                                                                                          cggcgCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGaagta                         >  1:283287/1‑149 (MQ=255)                                                                                                                           ggcgCATCAGCCGGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAaagaag                           <  2:126430/146‑1 (MQ=255)                                                                                                                             cgcATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTc                     >  2:749498/1‑150 (MQ=255)                                                                                                                             cgcATCACCCAGTCGAAAATAACCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCa                    <  2:907483/151‑1 (MQ=255)                                                                                                                                  cAGCCAGGCGAAAATAACCCCACCCACCACCCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCg               <  1:441971/151‑1 (MQ=255)                                                                                                                                      cggTCGGAAAAAACCCCCCCCAGCCACCCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAgg                  <  2:827703/142‑1 (MQ=255)                                                                                                                                        ccccctcccaatCCC‑CCCACCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTg            <  1:1062430/138‑1 (MQ=255)                                                                                                                                          cGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATAc        >  2:67977/1‑150 (MQ=255)                                                                                                                                            aaaaTATCCCCACCAACCCCACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGt     <  2:1157365/150‑1 (MQ=255)                                                                                                                                             aaaaaaCCCCCCCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGt     <  2:1057674/150‑1 (MQ=255)                                                                                                                                             aaaaaaCCCCCCCAAGCCCACCGCCGATAACACCGCAAGCCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGGCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATAc        <  1:360273/147‑1 (MQ=255)                                                                                                                                               ataatCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACt  >  2:748848/1‑151 (MQ=255)                                                                                                                                                   |                                                                                                                                                   CAGCGCCATAAAGCCGATGATCATCCACTCAACGTTGACGTAGTTGCAGAACACCATCACCATCGACAGCAACATGCCCATTACGATCGGTGTTTTACGCGCAATGTTCAGCGATCCCGTGCGGCGCATCAGCCAGTCGGAAATAATCCCACCCAGCACACCGCCGATAAAACCGCAAACCGCCGGAACGGAAGCCACAAAGCCCGCTTTCAGAATCGACATCCCGCGTGCCTGCACCAGATAAACCGGGAACCAGGTAATAAAGAAGTAAGTCAGGGCGTTGATACAGTACT  >  NC_000913/2921027‑2921319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑