| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 357,880 | 0 | T | C | 45.2% | 48.6 / 12.6 | 42 | K271E (AAA→GAA) | cynR | transcriptional activator of cyn operon; autorepressor |
| Reads supporting (aligned to +/- strand): ref base T (15/8); new base C (0/19); total (15/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.72e-06 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAA > NC_000913/357730‑358020 | aCTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTAttttt > 1:878994/1‑151 (MQ=255) cTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTc > 1:68170/1‑151 (MQ=255) aGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTccgcc < 2:75357/150‑1 (MQ=255) gCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCcgccgc > 2:914081/1‑150 (MQ=255) ccTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaa < 2:486288/151‑1 (MQ=255) gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGcaaca < 2:189099/150‑1 (MQ=255) tAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACaaa > 2:534877/1‑150 (MQ=255) aaGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAc < 2:136044/151‑1 (MQ=255) aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAcc < 1:270440/151‑1 (MQ=255) ttAGCGACTCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAA‑CCGCGCATTTATCCACCGCCATGTGCAAAAATCCCTTC‑TCGGCGGCTGTCTGCCAGGTATTTTTCCGCCGcaaca < 1:1212673/142‑1 (MQ=17) gTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgcc > 2:524818/1‑151 (MQ=255) tAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAAccgccg > 1:1164024/1‑151 (MQ=255) gcATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTtct > 1:726784/1‑151 (MQ=255) aTTCCAGCCGCAAACCTGTGACAGCGGCTACCTTGATTCAATTCCGCCAACAA‑CCGCGCATTTATCCACCGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGGCAGCTCTTTTTCCGCCGCAACAAAACCGCCGTTCGCTCCatgcg > 1:695910/1‑146 (MQ=21) ccccaccccTGTGTCAGCGGCCCCCGTTATTCTTCCCCACCAACAA‑CCGCGCATTTACCCAGCCCTTTGTACAAAAATGCGTTC‑GGGGCGCTTGTCGCCCAGTTTTTTTCCCCCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgg < 2:788887/142‑1 (MQ=255) ccGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggg > 2:405301/1‑151 (MQ=255) aCCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCgggggga > 2:822801/1‑145 (MQ=255) tgtCAGCGCCCACCGTGTTTCATTCCCGCCAACAC‑CCGCGCATTTATCCAACCCCATGTGAAAAAATGCCTGC‑GGGGCGGCTGTCTCCCAGCTATTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑aaa < 2:16814/149‑1 (MQ=255) cAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAG‑aaaaa > 2:413865/1‑146 (MQ=255) aGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGGAAAG‑AAAAAACtt > 2:447714/1‑151 (MQ=255) gCGGCTCCCGTGTTTCATTCCCCCCAACAC‑CCGCGCATTTACCCACCCCCTTGTAAAAAAATCCCTGC‑GGGGCGGCTTTCTGCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCt < 2:1013703/149‑1 (MQ=255) ggCTCCCGTGATTCATTCCCGCCAACAC‑CCGCGCTTTTACCCACCGCCATGTGAAAAAATGCCTTC‑GGGGGGGCTGTCTGCCAGTTTTTTTCCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCt < 2:667242/147‑1 (MQ=25) gcctcccttGATTCTTTCCCCCCAACAC‑CCGCCTATTTACCCAACGCCTTTTGAAAAAATGCCTCC‑GGGGCGCCTGTCTGCCGGCTTTTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCt < 2:421223/139‑1 (MQ=255) ccGTCATTTTTTCCCCCCAACAC‑CCGCATGTTTAACCACCGCTGTGGGAAAAAGCTCCTCC‑GCGGCGGCGTTGGGCCAGTTTTTTGCCCGCCGAAAAAAATCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGc < 1:758410/148‑1 (MQ=255) gtgtTTCTTTTCCGCCACCAC‑CCGCGCTTTTATCCACCCCCAGGAGAAAAAAGCCCTCC‑GGGGCGTCTGTCTCCCGGTTTTTTTTCCGCCGAAAAAAACCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAg < 1:560189/145‑1 (MQ=255) ttCTTTCCCCCCAACAC‑CCGCGATTTTACCCACCGCCATGGGAAAAAATCCCTCC‑GGGGGGGTTGTCTCCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCa < 2:222854/148‑1 (MQ=255) ttCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCGAGGGAAAAAACCTTTAGGCCCgcc > 2:97644/1‑146 (MQ=25) ttCATTCCCCCCAACAC‑CCGCGCTTTTACCCACCGCCATGTGCAAAAGGGCCTTC‑GCGGCGGCTTTCTGCCAGTTTTTTTCCCCCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCAtg < 1:709955/150‑1 (MQ=255) ttCATTCCCACCAACAC‑CCGCGCTTTTATCCACCCCCATGTCCAAAATTCCCTTC‑GCGGCGGCTGTCGGCCGGCTTTTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCAt < 2:169747/149‑1 (MQ=255) ttctttCCCCCAAAAACCCGCGCTTTTCCCCACCG‑CATTGGAAAAAAAGGCTTCGGGGGGGGCTGCCGCCCAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGt < 2:1002531/143‑1 (MQ=255) ttcccGCCAACAC‑CCGCGAATTTACCCACCCCAATGTGAAAAAATGCTTCG‑GCGGCGGCTGTCTGCCGGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCAtgttg < 2:1006975/146‑1 (MQ=255) cccccaaacac‑cCGCCCTTTTACCCACCCCCATGTCAAAAAATGCCTTG‑GGGCCGGCTTTCTGCCACCTTTTTTCCCGCCACAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTtgtgt < 2:436982/144‑1 (MQ=255) tttACCAAACGCCATGTAAAAAAATCCCTCC‑GGGGCGGCTGTCTGCCAGCTTTTTTCCCCCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCgctgct < 2:1146828/145‑1 (MQ=255) cccaccccctggtaaaaaatTGCCTCC‑GCGGCGGCTTTCGCCCAGTTTTTTTCCCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAt < 2:951034/135‑1 (MQ=255) ccaccccctggtgAAAAAAT‑CCTTCGGGGGCGGTTTTCTCCAAGTTTTTTTCCCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAAtg < 2:1099466/140‑1 (MQ=255) gtgAAAAAGCGCCTTC‑GCGGCGGCTGTCTGCCAGTTATTTTCCCGCCGCAAAAAAGCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGaa < 2:1101774/151‑1 (MQ=255) cAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAG‑GAAAAACATTTAACCCCCCTTGTTTTTTGGGAATCGCTGCTGGTGGCAAAGTGGGGAggg > 2:604906/1‑146 (MQ=255) ccTTC‑GCGGGGGCTGTCTCCCTGTTTTTTTTCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAAt < 1:273880/151‑1 (MQ=255) ggggcggcTGTCGGCCACCTATTTTCCCCCCCAAAAAAAGCCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCt < 1:440339/149‑1 (MQ=255) gcggcTGTCTGCCAGTTTTTTTCCCGCCGAAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTcc < 2:1043186/150‑1 (MQ=255) gcTTTCTCCCAGTTTTTTTCCCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAg < 2:468013/149‑1 (MQ=255) ccAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGGG‑AAAAAAATTTAAACCCGCCCTTTTTTGTGGGTATCGCTTCTGGGAACAATATGGAAAGGGGGGGGCGGGGGAGCAACGCCCGAACcgc > 2:802250/1‑147 (MQ=255) cagtttttttcCCGCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCa < 2:1190831/133‑1 (MQ=255) gCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGAAGAG‑AAAAAACTTTTAAACCCTCCTGTTTTGTGGGAATCGCTGCTGGTGACAAAGAGGGAAAGGGAGGGGGGGGGATCAAATCCCCAACCgcgccca > 2:145155/1‑146 (MQ=255) | ACTCGTAGGCCTGATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAA‑CCGCGCATTTATCCAACGCCATGTGCAAAAATGCCTTC‑GCGGCGGCTGTCTGCCAGCTATTTTTCCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGCAAGAG‑AAATAGCTTTAAGCCCGTCATGTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAA > NC_000913/357730‑358020 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |