Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,966,751	C→T	A112V (GCG→GTG)	rho →	transcription termination factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,966,751	0	C	T	100.0%	292.3 / NA	74	A112V (GCG→GTG)	rho	transcription termination factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (35/39);  total (35/39)

TTCCTCCGTTCCGC‑AGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGT  >  NC_000913/3966606‑3966898                                                                                                                                                     |                                                                                                                                                    ttCCT‑CGTTCCCCAAGACAGCTCCTCCCCCGCCGGTCCTGATGACATCTCCGTTCCCCTTACCA‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATTTCGGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTgctg                                                                                                                                                 <  1:1083796/150‑1 (MQ=255)  tcctccGTTCCGC‑AGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTgctgct                                                                                                                                               >  1:519177/1‑151 (MQ=255)  tcctccGTTCCGC‑AGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTgctgct                                                                                                                                               >  1:1004900/1‑151 (MQ=255)   cctccGTTCCGC‑AGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGctgctg                                                                                                                                              >  1:957937/1‑151 (MQ=255)         ttCCGC‑AGCCACCCCCTCCCTCGCCGGTCCTGATGACTTCTCCTTTCCCCCTAGCC‑AAAC‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGt                                                                                                                                         <  2:22203/150‑1 (MQ=255)         ttCCGC‑AGACAGCTCCTCCCCCGCCGGTCCTGATGACATCTACTTTCCCCCTAGCC‑AAAC‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAg                                                                                                                                          <  2:1040827/149‑1 (MQ=255)          tCCGC‑AGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGtt                                                                                                                                        >  2:869161/1‑150 (MQ=255)          tCCGC‑AGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGtt                                                                                                                                        >  2:571214/1‑150 (MQ=255)             gC‑AGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACg                                                                                                                                    >  2:925304/1‑151 (MQ=255)             gC‑AGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACg                                                                                                                                    >  2:865400/1‑151 (MQ=255)                  aCAGCTCCTCCCTCGCCGGTCCTGATGACATCTACGTTCCCCCTAGCA‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAg                                                                                                                                 <  2:526520/150‑1 (MQ=255)                   cAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGtt                                                                                                                               >  1:34908/1‑151 (MQ=255)                   cAGCCCCTCCCTCCCCGGTCTTGATGCCATCTACTTTTCCCCAACCC‑AAAT‑CCCCCTTTTCACCCCCCGCACTGGGGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGt                                                                                                                                         <  1:540977/141‑1 (MQ=255)                       tCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACt                                                                                                                           >  2:231432/1‑151 (MQ=255)                            ccTCGCCGGTCCTGATGACATCTACGTTCCCCCTACCC‑AAAC‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTc                                                                                                                         <  2:682655/148‑1 (MQ=255)                               cGCCGGTCCTGATGACTTCTACTTTCCCCCTACCC‑AAAC‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACa                                                                                                                     <  1:414278/149‑1 (MQ=255)                                  cGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCt                                                                                                                >  2:303426/1‑151 (MQ=255)                                  cGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCt                                                                                                                >  1:404544/1‑151 (MQ=255)                                      ccTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaa                                                                                                             >  1:901847/1‑150 (MQ=255)                                      ccTGATGACATCTACGTTCCCCCTACCA‑AAAC‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGa                                                                                                              <  2:46128/149‑1 (MQ=255)                                       cTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaaa                                                                                                            >  1:1012744/1‑150 (MQ=255)                                             gacaccgcctttCCCCCAACCAAAAC‑CCTCCGTTTCACCCTCCGCACTGGTGATCCCATCTCGGGTAAGATTCCCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGc                                                                                                        <  1:1176613/140‑1 (MQ=255)                                             aCATCTCCTTTCCCCCTACCA‑AAAC‑CCGCCGTTTAAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGcc                                                                                                       <  2:963528/149‑1 (MQ=255)                                                tttgcGTTCCCCCTAGCA‑AAAT‑CCGCCGTTTAACCCTCCGCACTGGTGATCCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCg                                                                                                     <  2:425287/144‑1 (MQ=255)                                                    actttCCCCCAGCC‑CAATCCCGCCGTTTCACCCTCCGCACGGGTGACCCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaac                                                                                                 <  1:75965/147‑1 (MQ=255)                                                      ttcccccTAGCA‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACg                                                                                                         <  2:103478/135‑1 (MQ=255)                                                       ttCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATc                                                                                           >  1:1145887/1‑151 (MQ=255)                                                       ttCCCCTAGCC‑AAAC‑CCGCCTTTTCACCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAAt                                                                                            <  2:858327/150‑1 (MQ=255)                                                         ccccTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCct                                                                                         >  2:956303/1‑151 (MQ=255)                                                         ccccTACCC‑AAAC‑CCGCCGTTTCACCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCct                                                                                         <  2:300811/151‑1 (MQ=255)                                                         ccccTACCA‑AAAT‑CCGCCGTTTCACCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCct                                                                                         <  2:426763/151‑1 (MQ=255)                                                          cccTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCctc                                                                                        >  1:331640/1‑151 (MQ=255)                                                              tacc‑AAATCCCGCCGTTTCACCCCCCGCGCGGTAAATACCTTTTCTGTTAAGATCCACCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATGct                                                                                         <  1:909111/145‑1 (MQ=255)                                                                 c‑AAAT‑CCGCCGTTTCAACCTCCGCACTCGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTg                                                                                    >  2:1148793/1‑148 (MQ=255)                                                                   aaaT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgaga                                                                                 >  2:290545/1‑150 (MQ=255)                                                                    aaT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAAc                                                                               >  2:54588/1‑151 (MQ=255)                                                                         cgccgTTTCACCCCCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTaa                                                                           <  2:231505/151‑1 (MQ=255)                                                                                   aCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGc                                                                  >  1:552290/1‑150 (MQ=255)                                                                                    ccTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc                                                                >  1:41941/1‑151 (MQ=255)                                                                                    ccTCCCCACTGGTGATACCTTCTCTGTTAACATACGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc                                                                <  1:536659/151‑1 (MQ=255)                                                                                        cGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCa                                                             >  2:1007912/1‑150 (MQ=255)                                                                                                taaTACCATCTCTGTTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActct                                                       <  1:359561/146‑1 (MQ=255)                                                                                                    aCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc                                                >  1:255936/1‑151 (MQ=255)                                                                                                    aCCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGc                                                >  1:613038/1‑151 (MQ=255)                                                                                                     ccgtctcGGGTAAGATGCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCg                                               <  1:364603/148‑1 (MQ=255)                                                                                                         ctctGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATg                                           >  1:121498/1‑151 (MQ=255)                                                                                                             gttAAGATCCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATgg                                          <  2:387015/146‑1 (MQ=255)                                                                                                             ggTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAAc                                       <  2:491853/151‑1 (MQ=255)                                                                                                               tAAAATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGa                                         <  1:1212185/147‑1 (MQ=255)                                                                                                                aaGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGt                                     <  1:868921/150‑1 (MQ=255)                                                                                                                 agtacgagccgccgAAAGAAGGTGAACGCGATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAATACGCCCGCAACAAAATCCTCTTTGAGAACTTAGCCCCGCTGCACGCAAACTCTCGTCTGCGTa                                             <  1:289997/133‑1 (MQ=255)                                                                                                                      cgcccgccGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTaa                                <  1:755584/149‑1 (MQ=255)                                                                                                                      cgcccgccGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACg                              <  1:177024/151‑1 (MQ=255)                                                                                                                      cgcccgccGAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACg                              >  1:58147/1‑151 (MQ=255)                                                                                                                         ccgccgAAAGAAGGTTAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAAc                               <  2:1176003/147‑1 (MQ=255)                                                                                                                         ccgccgAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGtt                           <  2:775767/151‑1 (MQ=255)                                                                                                                         ccgccgAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGtt                           <  2:171830/151‑1 (MQ=255)                                                                                                                          cgccgAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTc                          <  1:303358/151‑1 (MQ=255)                                                                                                                          cgccgAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTc                          >  2:163735/1‑151 (MQ=255)                                                                                                                              gAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTAc                       <  2:497066/150‑1 (MQ=255)                                                                                                                              gAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACt                      >  2:678932/1‑151 (MQ=255)                                                                                                                              gAAAGAAGGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACt                      >  1:1226124/1‑151 (MQ=255)                                                                                                                                aagaagGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTg                     >  2:524687/1‑150 (MQ=255)                                                                                                                                  gaagGTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAg                  <  2:569919/151‑1 (MQ=255)                                                                                                                                      gTGAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGAttt              >  2:530594/1‑151 (MQ=255)                                                                                                                                        gAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTa             <  2:709062/150‑1 (MQ=255)                                                                                                                                        gAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTa             <  2:902102/150‑1 (MQ=255)                                                                                                                                        gAACGCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTa             <  1:526057/150‑1 (MQ=255)                                                                                                                                            gCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACt          >  2:173717/1‑149 (MQ=255)                                                                                                                                            gCTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGc        <  2:1129850/151‑1 (MQ=255)                                                                                                                                             cTATTTTGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCt       >  2:824220/1‑151 (MQ=255)                                                                                                                                                ttttGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTcgc    >  1:582326/1‑151 (MQ=255)                                                                                                                                                 tttGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTcgc    <  1:683490/150‑1 (MQ=255)                                                                                                                                                  ttGTGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGt  >  2:451350/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                    TTCCTCCGTTCCGC‑AGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCC‑AAAT‑CCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTACTGAAGATTTAACTGCTCGCGT  >  NC_000913/3966606‑3966898

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑