Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,604,344	0	C	T	39.2%	70.4 / 19.7	51	S62F (TCT→TTT)	bglJ	bgl operon transcriptional activator
Reads supporting (aligned to +/- strand):  ref base C (8/23);  new base T (20/0);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 4.33e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGG  >  NC_000913/4604196‑4604494                                                                                                                                                     |                                                                                                                                                        cTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTtttttttt                                                                                                                                                       >  1:814922/1‑151 (MQ=255)   cATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATtttttcttt                                                                                                                                                      <  2:66165/150‑1 (MQ=255)    aTAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCAtttttttttttt                                                                                                                                                    >  2:594667/1‑151 (MQ=255)       gAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGCCATTttttttttttct                                                                                                                                                  >  2:78761/1‑150 (MQ=255)              gCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATga                                                                                                                                           <  2:64093/150‑1 (MQ=255)               cGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATgaga                                                                                                                                         <  2:153153/151‑1 (MQ=255)                  cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGGGaa                                                                                                                                        >  1:884203/1‑149 (MQ=255)                  cATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCATGAGAAg                                                                                                                                       >  1:891881/1‑150 (MQ=255)                         agtaTCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAgcg                                                                                                                                 >  1:11059/1‑149 (MQ=255)                             tCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCaga                                                                                                                             <  2:395204/149‑1 (MQ=255)                                  aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAggg                                                                                                                        >  1:36314/1‑149 (MQ=255)                                  aTTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGAt                                                                                                                      <  2:998794/151‑1 (MQ=255)                                       gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGATTATCt                                                                                                                 >  1:607998/1‑151 (MQ=255)                                       gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTTTCCTGAGAAGTGAGGGCAGAGAGGGATTATCt                                                                                                                 >  2:952369/1‑151 (MQ=255)                                       gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCt                                                                                                                 <  1:615467/151‑1 (MQ=255)                                       gagTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCt                                                                                                                 <  2:291359/151‑1 (MQ=255)                                         gTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCGCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTg                                                                                                               <  2:383480/151‑1 (MQ=255)                                           ttATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGc                                                                                                              <  1:188456/150‑1 (MQ=255)                                            tATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGAGAGCGCAGAGAGGGGTTTTCTTGCct                                                                                                            >  2:328770/1‑151 (MQ=255)                                                cAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgact                                                                                                        <  2:371158/151‑1 (MQ=255)                                                 aGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGGGAAGGGAGCGCAGAGAGGGGTTATCTTGTcttactg                                                                                                       >  2:811593/1‑151 (MQ=255)                                                  gAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCctgactga                                                                                                      <  1:87466/151‑1 (MQ=255)                                                      gTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGGCATGGGAAGGGCGCGGAGAGAGGGAGTATTTTGTCtgaatgaa                                                                                                     >  2:24975/1‑148 (MQ=255)                                                               tAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCCTGAGAAGGGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTa                                                                                          >  1:240200/1‑149 (MQ=255)                                                                aaCCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGAAGTGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCGA‑TTaa                                                                                         >  1:11401/1‑149 (MQ=255)                                                                     ttATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTTCCCTGAGAAGGGAGCGGAGAGAGGGGTTTTCTTTCCTGGCGGGAATTGCGG‑TTAAAttt                                                                                     >  1:227193/1‑148 (MQ=255)                                                                       ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCcgc                                                                                 <  2:496676/150‑1 (MQ=255)                                                                       ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCcgc                                                                                 <  1:1076014/150‑1 (MQ=255)                                                                       ataAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCcgc                                                                                 <  1:19317/150‑1 (MQ=255)                                                                        taAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCcgcg                                                                                >  2:942210/1‑150 (MQ=255)                                                                          aGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGTGAGCGCCGAGAGGGGTTATCTTGCCTGACTGAAATGGCGG‑TTAAGTTTTcgcg                                                                                >  1:479950/1‑148 (MQ=255)                                                                             tCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGAAGGGAGCGCAGAGAGGGGTTATTTTTCCTGACTGAACTTGCGG‑TTAAGTTTTCGCGTAccc                                                                           >  1:693306/1‑150 (MQ=255)                                                                                aTACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCg                                                                       <  2:36305/151‑1 (MQ=255)                                                                                  aCCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTCTGCCATGAGAAGTGAGCGGAGAGAGGGGTTATCTTTGCTTACTTTACTTGCGATTTAAGTTT‑CGCCTACCCGGCggt                                                                     >  2:1008704/1‑149 (MQ=255)                                                                                     tataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTTTGCCCTGTGAAGGGAGCGCAGAGAGGGGTTATCTTGCCTGACTGAACTGGCGG‑TTTAGTTTTCGCGTACCCGGCGttt                                                                    >  2:589841/1‑149 (MQ=255)                                                                                      ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTGCCATGAGGAGGGAGGGCAGAGAGGGGTTTTCTTTCCTGACTGAAATGGCGA‑TTAAGTTTCCGCCGACCCGGCGgtt                                                                    >  2:282095/1‑148 (MQ=255)                                                                                      ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTTCGCGTACCCGGCGttt                                                                    >  1:881985/1‑148 (MQ=255)                                                                                      ataCCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGt                                                                 <  1:26419/151‑1 (MQ=255)                                                                                            gTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGc                                                           <  2:501039/151‑1 (MQ=255)                                                                                                      aTTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGgatgatgat                                                 <  2:972636/151‑1 (MQ=255)                                                                                                                gCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCt                                        <  2:503713/150‑1 (MQ=255)                                                                                                                 ccATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGAGGAGTGAGGGCAGAGAGGGGTTATCTTTCCCGGCTGAACTGGCGG‑TTTAGTTTTCGCGGGCCCGGCGGTTTGTTTTTTGGGGTGATGATATTTAAGCTc                                       >  1:505158/1‑150 (MQ=255)                                                                                                                 ccATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCg                                      <  1:256248/151‑1 (MQ=255)                                                                                                                   aTGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGc                                    <  1:1030459/151‑1 (MQ=255)                                                                                                                    tGTCGCGGATCTCGTTTGCGGCGGTCCTTTTTTTTTTTTCTGCCCTTAGAAGTGAGCGCCGGGGGGGGTTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGGACCCGGCGTTTTGTTATTTCGGATGTTGATATTTAAGCTCg                                      >  2:938919/1‑148 (MQ=255)                                                                                                                       cgcgGATCTCGTTTGCGGCGGTCATTTTTTTTTTTTTTTCCCTGGGGAGTGAGCGCAGGGGGGGATTATTTTGCCTGTCTGGAATGGCGA‑TTAAGGTTTCGCGGAACCGGCGTTTTGTTTTTGTGGGTGATGGTATTTAAGCCCGGCTggt                                >  2:568912/1‑149 (MQ=255)                                                                                                                          ggATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTgg                             <  2:814664/151‑1 (MQ=255)                                                                                                                                cGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGt                            <  2:891595/146‑1 (MQ=255)                                                                                                                                 gTTTGCGGCGGTCATTTTTTCTTTTTTTTGCATGAGAAGGGAGCGCAGAGAGGGATTATCTTTTCTTTCTGAACTGGCGA‑TTAAGTTTTCGCGTACCCGGCGGTTTGTTTTTGTGGATGATGATATTGAAGCTCGGCTGCTTGGGTTAtt                       >  2:46111/1‑150 (MQ=255)                                                                                                                                  tttGCGGCGGTCATTTTTTCTTTTTTTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTTCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGGTGATGATATTGAAGCTCGGCTGGTTGGTTCATTGt                     >  1:643802/1‑151 (MQ=255)                                                                                                                                     gcggcgGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGc                  <  2:192803/151‑1 (MQ=255)                                                                                                                                           gTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCAc            >  2:258200/1‑151 (MQ=255)                                                                                                                                             cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGCGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCAt               >  2:1054794/1‑146 (MQ=255)                                                                                                                                             cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg          >  1:570223/1‑151 (MQ=255)                                                                                                                                             cATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCg          >  1:1092040/1‑151 (MQ=255)                                                                                                                                                tttttctttttcTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCt        <  1:51168/150‑1 (MQ=255)                                                                                                                                                 ttttctttttctGCTATGAGAAGTGAGCGCAGAGAAGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGCAGGATGACATTGGAGCGCGGCTGATTGGTTCATTGTCGGCATCAGCGCTg       <  2:1074639/150‑1 (MQ=255)                                                                                                                                                    tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGAc    <  2:641300/150‑1 (MQ=255)                                                                                                                                                    tctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACg   >  1:50862/1‑151 (MQ=255)                                                                                                                                                     ctttttctGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTCTTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACgg  >  2:350295/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                        CTCATAGAAAAATGCGTCATGAGTAGTATCGGTATTGAGAGTTTATTCAGAAAGTTTGCGGGTAACCCTTATAAGCTCCATACCTATACCAGTCAGGAGTCATTTCAGGATGCCATGTCGCGGATCTCGTTTGCGGCGGTCATTTTTTCTTTTTCTGCCATGAGAAGTGAGCGCAGAGAGGGATTATCTTGCCTGACTGAACTGGCGA‑TTAAGTTTCCGCGTACCCGGCGTTTAGTTATTGCGGATGATGATATTGAAGCTCGGCTGATTGGTTCATTGTCGCCATCACCGCTGGACGG  >  NC_000913/4604196‑4604494

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑