Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,153,573	0	T	G	39.3%	100.7 / 31.9	61	Y92D (TAC→GAC)	pabC	4‑amino‑4‑deoxychorismate lyase component of para‑aminobenzoate synthase multienzyme complex
Reads supporting (aligned to +/- strand):  ref base T (5/32);  new base G (24/0);  total (29/32)
Fisher's exact test for biased strand distribution p-value = 2.00e-12
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGCAGGTATTAA  >  NC_000913/1153433‑1153718                                                                                                                                             |                                                                                                                                                    gACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGGGCAGgacaat                                                                                                                                           >  1:994879/1‑149 (MQ=255)   cTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGACAACACAt                                                                                                                                            >  1:199166/1‑148 (MQ=255)     aCAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGa                                                                                                                                         <  1:1037643/149‑1 (MQ=255)      cAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCTGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCTCGGTAGTGGCGGGCGAGGGTACAGCACATTGAAc                                                                                                                                       <  2:12673/150‑1 (MQ=255)       aGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGGGGGGGCAGCACAATGaa                                                                                                                                        >  1:852122/1‑148 (MQ=255)       aGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAg                                                                                                                                     <  1:742507/151‑1 (MQ=255)         gATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCg                                                                                                                                   <  1:1041414/151‑1 (MQ=255)            gCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGGACAGCACATTGAAAAGCgg                                                                                                                                  >  1:454755/1‑149 (MQ=255)                gTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCgg                                                                                                                             <  2:6393/150‑1 (MQ=255)                   aGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGGGGGGGCAGCACCTTTAAAAGCGGAACgg                                                                                                                             >  1:992617/1‑147 (MQ=255)                      ggTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAAcgcg                                                                                                                      <  2:209503/151‑1 (MQ=255)                            tgatTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGGCCGCACAATTAACAGCGGACCGGCAACGCGGGtttt                                                                                                                >  1:1012581/1‑149 (MQ=255)                             gatTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATtctc                                                                                                               <  2:128600/151‑1 (MQ=255)                              atTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGGGGGGAAAACACAATGAACAACGGACCGGCAACGCggggttt                                                                                                                >  1:440077/1‑144 (MQ=255)                                ttCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGGACGCACACTGGACAACGGACCggggacggggggt                                                                                                                  >  2:404465/1‑134 (MQ=255)                                  ccTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGt                                                                                                         <  1:756225/151‑1 (MQ=255)                                  ccTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGGGCAGCACATTTAAAAACGGGGCGGCGACGCGGGTtctct‑c                                                                                                            >  2:505398/1‑148 (MQ=255)                                     gtgACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAc                                                                                                      <  1:505578/151‑1 (MQ=255)                                      tgACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGACAGGACAATGTATAGAAGAACGGGAAAGCGGGAGCTCT‑cccct                                                                                                        >  2:169027/1‑145 (MQ=255)                                           tCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCtt                                                                                                <  1:583225/150‑1 (MQ=255)                                              ggCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATcc                                                                                            <  2:400421/151‑1 (MQ=255)                                               gCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGGGGGGGCAGGACATTTAAAAACGGGCCGGCCACACGGGTTTTCT‑CCCTTAC‑gggctat                                                                                              >  1:354942/1‑143 (MQ=255)                                                 cTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGAGGGGAGAGCGCACTGGAAAACGGAACGGGCAAGCGGGGTCTCT‑CCGCTAC‑gggctattt                                                                                            >  2:520140/1‑141 (MQ=255)                                                   cAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGca                                                                                        <  1:53806/150‑1 (MQ=255)                                                        tGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACAtt                                                                                    <  1:914462/149‑1 (MQ=255)                                                         gAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGGGGGGGGAGAAAAATTAACAGCGGGCCGGGCAAGCGGGGTCTCT‑CCggttaggccttttc                                                                                             >  2:818869/1‑127 (MQ=255)                                                             aaGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGGACAGCACAATGAACAGCGGACCGGCAACGCGGGTTTTCT‑CCCTTTC‑GGCCTTTTCTGCCCATTACGAcc                                                                              >  1:820978/1‑150 (MQ=255)                                                                 gaTGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTg                                                                         <  2:1044900/151‑1 (MQ=255)                                                                    gAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGt                                                                      <  2:523869/151‑1 (MQ=255)                                                                     aaaaCGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTa                                                                     <  1:414283/151‑1 (MQ=255)                                                                      aaaCGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTaa                                                                    <  1:730943/151‑1 (MQ=255)                                                                               cggcagAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGAt                                                           <  1:304903/149‑1 (MQ=255)                                                                                agcagAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGAtt                                                          <  2:548866/151‑1 (MQ=255)                                                                                 gcagAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGGGGGGGGACAGCACATTGAAAAACGGGCCGGGAAAGCGGGGTTTCT‑CCGTTTCGGGCTTTTCC‑GCCCCTTAACACCGTTTTTGTGAAGAgggg                                                             >  1:1082575/1‑147 (MQ=255)                                                                                   agAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGACAGCACATTGAAAAACGGGGCGGCCAAGCGGGGTCTCTCCCCTTCG‑GGCTTTTCC‑GCACATTTCGACCGTTTTTGTAAAGAgggg                                                             >  1:201447/1‑145 (MQ=255)                                                                                     aaCAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGGAAAGCACAATGAAAAGCGGACCGGCAACGCGGAGTCTCT‑CCCTTAC‑CGCTTATCCTGCCCATTACGACCGGTTGCGTAACGAGGGGGTTAAGtt                                                     >  1:473147/1‑151 (MQ=255)                                                                                           aaaaTGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCt                                               <  2:973190/151‑1 (MQ=255)                                                                                                ggTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGACAGCACATTTAAAAGCGGGCCGGGCACGCGGGTTTTCC‑CCCTTAC‑GGCTTATTCTTCCCATTACCACCGTTTTCGTAACGAGGGGGTTACGTTTGGGCTaa                                             >  1:870533/1‑148 (MQ=255)                                                                                                   gtgCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGGGAGGACAATGACAAACGGGGAGGCAACCCGGGGTGTCT‑CCGGTAA‑GGCGTTTTCTCCACAATTCGAACGCTTGCGGATCGGGGGGAGTATGTTGgggcctcaccccg                                       >  2:698426/1‑142 (MQ=255)                                                                                                        gAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGAGAGGACAATTAACAACCGACCGGCCACGCGGGGTCTCT‑TCCTTTC‑GGCTGATTCTTCACCTTTCTACCGCTTGCGTGACGAGGGGGGTTAGTTGGCGCCAAACCCGGCGCgg                                  >  2:334368/1‑151 (MQ=255)                                                                                                        gAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCg                                   <  2:695289/150‑1 (MQ=255)                                                                                                        gAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCg                                   <  2:208367/150‑1 (MQ=255)                                                                                                         aaaGTCGTGATCAGTCGCGGTAGTGGCGGGCGGGGGGAAAGGACAATTAACAGCGGGCCGGCCAAGCGGGTTTTCT‑CCCTTTC‑CGCTTATTCTGCACATTAAGACCGGTTTGGTAAAGAGGGGGGTATGTTGGGGCTAAACCCggggggg                                  >  1:188470/1‑145 (MQ=255)                                                                                                          aaGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGGAAAGCACATTTAAAAGCGGGACGGCAACGCGGGTTTTCT‑CCGTTAC‑GGCCTTTTCTGCACATTACGACCGTTTTTGTAACGAGGGGGGTACGTTGGCGCCAAACCCCGGGCgg                                  >  1:709855/1‑149 (MQ=255)                                                                                                              cGTGATCAGTCGCGGTAGTGGCGGGCGAGGGGACAGCACATTTAAAAGCGGGCCGGCAAAGCGGGTTCTCT‑CCCTTAC‑GGGTTATCCTTCACATTACGACCGTTTGCGGAAAGAGGGGGTTACGTTGGCGCTCAGCCCCGGGCGGGTgggg                            >  1:719834/1‑151 (MQ=255)                                                                                                                tGATCAGTCGCGGTAGTGGCGGGGGGGGGGACAGAACAATGAAAAACGGGCCGGCAACCCGGGGTTTCT‑CCGTTTC‑CGCTTATCCTGCCCAATACGAACGGTTTCGGAACGAGGGGGGTAAGTTTGCGCTAAACCCCGGGCGGCCgggggg                          >  1:1060314/1‑149 (MQ=255)                                                                                                                    cAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAAt                      <  2:1030187/151‑1 (MQ=255)                                                                                                                      gTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATcc                    <  1:474551/151‑1 (MQ=255)                                                                                                                        cgcgGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTc                  <  1:740568/151‑1 (MQ=255)                                                                                                                          cgGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTCAt                <  1:703445/151‑1 (MQ=255)                                                                                                                            gTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTCATCt              <  1:1076118/151‑1 (MQ=255)                                                                                                                                 ggcgggcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGc           >  2:1098970/1‑149 (MQ=255)                                                                                                                                 ggcgggcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGCAg         <  1:46846/151‑1 (MQ=255)                                                                                                                                  gcgggcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGCAgg        >  2:886127/1‑151 (MQ=255)                                                                                                                                    gggcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGCAGGTa      <  2:989170/151‑1 (MQ=255)                                                                                                                                      gcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGCAGGTAtt    <  2:994556/151‑1 (MQ=255)                                                                                                                                      gcgAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGCAGGTAtt    >  1:390686/1‑151 (MQ=255)                                                                                                                                        gAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGCAGGTATTa   >  1:472555/1‑150 (MQ=255)                                                                                                                                        gAGGCTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGTGGCGCAATCCTCATCTTGCAGGTATTaa  <  2:362200/151‑1 (MQ=255)                                                                                                                                        gAGGATACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGCAGGTATTaa  <  2:812611/151‑1 (MQ=255)                                                                                                                                         aGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGCAGGTATTaa  >  2:387950/1‑150 (MQ=255)                                                                                                                                             |                                                                                                                                                    GACTACAGGATGCTTGTCAGCGGTTGATGATTTCCTGTGACTTCTGGCCTCAGCTTGAACAAGAGATGAAAACGCTGGCAGCAGAACAGCAAAATGGTGTGCTGAAAGTCGTGATCAGTCGCGGTAGTGGCGGGCGAGGGTACAGCACATTGAACAGCGGACCGGCAACGCGGATTCTCT‑CCGTTAC‑GGCTTATCCTGCACATTACGACCGTTTGCGTAACGAGGGGATTACGTTGGCGCTAAGCCCGGTGCGGCTGGGGCGCAATCCTCATCTTGCAGGTATTAA  >  NC_000913/1153433‑1153718

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑