Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	954,638	C→T	intergenic (‑172/+234)	focA ← / ← ycaO	formate channel/ribosomal protein S12 methylthiotransferase accessory factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	954,638	0	C	T	100.0%	313.9 / NA	82	intergenic (‑172/+234)	focA/ycaO	formate channel/ribosomal protein S12 methylthiotransferase accessory factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (45/37);  total (45/37)

GGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAATTACAATTATTTTATTAATGC  >  NC_000913/954490‑954783                                                                                                                                                     |                                                                                                                                                  ggCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATtaataa                                                                                                                                                 <  2:311332/151‑1 (MQ=255)  gCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATtaataa                                                                                                                                                 >  2:5686/1‑150 (MQ=255)  gCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATaaa                                                                                                                                                <  2:206511/151‑1 (MQ=255)    gacgaGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATg                                                                                                                                              >  1:5286/1‑151 (MQ=255)    gacgaGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATg                                                                                                                                              >  1:227608/1‑151 (MQ=255)      cgaGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATg                                                                                                                                              >  2:824887/1‑149 (MQ=255)      cgaGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAg                                                                                                                                            <  2:527853/151‑1 (MQ=255)      cgaGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAg                                                                                                                                            <  1:273682/151‑1 (MQ=255)       gagaTACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGt                                                                                                                                           >  1:16234/1‑151 (MQ=255)        agaTACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGtt                                                                                                                                          >  1:259590/1‑151 (MQ=255)              tAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAAttt                                                                                                                                    >  1:521799/1‑151 (MQ=255)               aaCAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAAtttt                                                                                                                                   >  2:489515/1‑151 (MQ=255)                aCAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAAttttt                                                                                                                                  <  2:848118/151‑1 (MQ=255)                   aaGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTcc                                                                                                                               >  1:343927/1‑151 (MQ=255)                    aGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCg                                                                                                                              <  2:981484/151‑1 (MQ=255)                    aGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCg                                                                                                                              <  2:777661/151‑1 (MQ=255)                    aGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGATTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCg                                                                                                                              <  2:642654/151‑1 (MQ=255)                      cATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGc                                                                                                                             >  2:609232/1‑150 (MQ=255)                       aTTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCAt                                                                                                                           <  2:351567/151‑1 (MQ=255)                       aTTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCAt                                                                                                                           >  1:436324/1‑151 (MQ=255)                        ttATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCAtc                                                                                                                          >  1:431909/1‑151 (MQ=255)                            aGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCAtcctcc                                                                                                                      >  1:565774/1‑151 (MQ=255)                            aGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCAtcctcc                                                                                                                      <  1:126684/151‑1 (MQ=255)                            aGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCAtcctc                                                                                                                       <  1:144996/150‑1 (MQ=255)                             gATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCg                                                                                                                     <  2:255703/151‑1 (MQ=255)                                 agaAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTa                                                                                                                  >  2:401510/1‑150 (MQ=255)                                   aaaTTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTaaaa                                                                                                               <  2:1012026/151‑1 (MQ=255)                                     aTTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAAc                                                                                                              >  2:262646/1‑150 (MQ=255)                                        gATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGtt                                                                                                          >  1:163631/1‑151 (MQ=255)                                             aGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGttaatta                                                                                                     <  1:184289/151‑1 (MQ=255)                                             aGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGttaatt                                                                                                      <  1:43262/150‑1 (MQ=255)                                               aTCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGttaattaa                                                                                                    >  2:529991/1‑150 (MQ=255)                                               aTCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTaaa                                                                                                   <  2:848893/151‑1 (MQ=255)                                                   tatCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAggg                                                                                               >  2:478980/1‑151 (MQ=255)                                                   tatCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAggg                                                                                               >  1:12050/1‑151 (MQ=255)                                                         gCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATc                                                                                         >  2:961694/1‑151 (MQ=255)                                                           cTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATc                                                                                         >  1:865840/1‑149 (MQ=255)                                                               cTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCg                                                                                    >  1:192793/1‑150 (MQ=255)                                                                 tataGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAAt                                                                                 >  2:303503/1‑151 (MQ=255)                                                                    aGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATaa                                                                               >  1:20549/1‑150 (MQ=255)                                                                            aaCTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAat                                                                      <  1:1044676/151‑1 (MQ=255)                                                                             aCTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAat                                                                      >  1:339008/1‑150 (MQ=255)                                                                                   aTGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATatcgatc                                                               <  2:638473/151‑1 (MQ=255)                                                                                          ttATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGtattt                                                         <  1:962009/150‑1 (MQ=255)                                                                                          ttATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGtattt                                                         >  1:618689/1‑150 (MQ=255)                                                                                            aTGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGtatttatt                                                      <  1:165247/151‑1 (MQ=255)                                                                                            aTGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATAGCGATCGtatttatt                                                      <  2:903196/151‑1 (MQ=255)                                                                                             tGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGtatttatt                                                      >  2:812727/1‑150 (MQ=255)                                                                                                 aaTACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAAt                                                  >  1:115139/1‑150 (MQ=255)                                                                                                  aTACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATAc                                                >  1:458860/1‑151 (MQ=255)                                                                                                    aCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACaa                                              <  2:941292/151‑1 (MQ=255)                                                                                                    aCGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACa                                               <  1:839046/150‑1 (MQ=255)                                                                                                      ggAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAAt                                            <  2:944812/151‑1 (MQ=255)                                                                                                      ggAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAAt                                            >  1:237605/1‑151 (MQ=255)                                                                                                        aGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAAtt                                           >  2:206202/1‑150 (MQ=255)                                                                                                        aGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAAtt                                           >  1:974040/1‑150 (MQ=255)                                                                                                           aaaTATTTGATTATCCAAATAAAAATAAATTTTAAAAATTGATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTAcc                                        <  1:653365/150‑1 (MQ=255)                                                                                                           aaaTATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTAcc                                        <  1:273651/150‑1 (MQ=255)                                                                                                           aaaTATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCg                                       <  1:986553/151‑1 (MQ=255)                                                                                                             atatTTGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGat                                     <  1:5118/151‑1 (MQ=255)                                                                                                                  tGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATAttt                                 >  2:224503/1‑150 (MQ=255)                                                                                                                  tGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATAttt                                 <  2:431748/150‑1 (MQ=255)                                                                                                                  tGATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATAttt                                 >  1:990620/1‑150 (MQ=255)                                                                                                                   gATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTa                                <  1:237362/150‑1 (MQ=255)                                                                                                                   gATTATCCAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTa                                >  2:62170/1‑150 (MQ=255)                                                                                                                         ccAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAGCCTtat                         <  1:477014/151‑1 (MQ=255)                                                                                                                         ccAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTtat                         <  1:430773/151‑1 (MQ=255)                                                                                                                         ccAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTtat                         >  1:1032137/1‑151 (MQ=255)                                                                                                                         ccAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTtat                         >  1:26291/1‑151 (MQ=255)                                                                                                                         ccAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTtat                         >  1:138552/1‑151 (MQ=255)                                                                                                                          cAAATAAAAATAAATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTtata                        <  2:429819/151‑1 (MQ=255)                                                                                                                           aaataaaaataaATTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTACAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTtata                        >  2:648386/1‑150 (MQ=255)                                                                                                                             ataaaaataaaTTTTAAAAATTAATAAATGGGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAAtt                     >  2:344040/1‑151 (MQ=255)                                                                                                                              taaaaataaaTTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAAtt                     >  1:680666/1‑150 (MQ=255)                                                                                                                                 aaataaatTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAATTACaa                 <  1:1101735/151‑1 (MQ=255)                                                                                                                                  aataaatTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAATTACAat                <  1:904161/151‑1 (MQ=255)                                                                                                                                  aataaatTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAATTACAat                <  1:991103/151‑1 (MQ=255)                                                                                                                                   ataaatTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAATTACAatt               >  2:422611/1‑151 (MQ=255)                                                                                                                                      aatTTTAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAATTACAattatt            >  1:824913/1‑151 (MQ=255)                                                                                                                                         tttAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAATTACAAttatttt          >  2:735859/1‑150 (MQ=255)                                                                                                                                          ttAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAATTACAAttatttta         <  1:930530/150‑1 (MQ=255)                                                                                                                                           tAAAAATTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAATTACAAttattttatt       >  2:36718/1‑151 (MQ=255)                                                                                                                                             aaaaTTAATAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAATTACAATTATTTTATTaa     <  1:1017257/151‑1 (MQ=255)                                                                                                                                                 ttAATAAATGAGTTGAGTTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAACGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAATTACAATTATTTTATTAATGc  >  2:978568/1‑150 (MQ=255)                                                                                                                                                     |                                                                                                                                                  GGCGACGAGATACTAACAAAGCATTATAGATGAGAAATTGATATAGATCATATCTCGCCTGGCTTATAGGCCCGTAACTCGCATGGTTTTTATGCAAATACGGAGTAAATATTTGATTATCCAAATAAAAATAAATTTTAAAAATTAACAAATGAGTTGAATTTTTTCCGCATCCTCCGCTAAAACAGTTAATTAAAAGGGAGCATCAGGCGAATAAAGTAACAATATCGATCGTATTTATTAAATACAAATTACCGATATTTAACCTTATAATTACAATTATTTTATTAATGC  >  NC_000913/954490‑954783

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑