Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 1,768,309 A→T V126E (GTG→GAG)  ydiJ ← putative FAD‑linked oxidoreductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,768,3090AT98.5% 258.0 / ‑3.9 70V126E (GTG→GAG) ydiJputative FAD‑linked oxidoreductase
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base T (34/33);  total (35/35)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.18e-01

CTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCACCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTGCCGCCGCCGCGGGGGGTAAAGATCAGCGAT  >  NC_000913/1768163‑1768455
                                                                                                                                                  |                                                                                                                                                  
cTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGGCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTcccc                                                                                                                                                >  2:500415/1‑151 (MQ=255)
cTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTcccc                                                                                                                                                >  1:676770/1‑151 (MQ=255)
  gACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTcccc                                                                                                                                                <  1:58772/149‑1 (MQ=255)
  gACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTccc                                                                                                                                                 <  1:212996/148‑1 (MQ=255)
  gACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTcc                                                                                                                                                  <  1:222399/147‑1 (MQ=255)
  gACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCgg                                                                                                                                              >  1:596361/1‑151 (MQ=255)
   aCGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGc                                                                                                                                             >  1:924457/1‑151 (MQ=255)
       tttGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTc                                                                                                                                          <  2:976774/150‑1 (MQ=255)
         tGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCaa                                                                                                                                        <  1:115744/150‑1 (MQ=255)
          gCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAAcg                                                                                                                                      <  1:48033/151‑1 (MQ=255)
           ccATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAAcg                                                                                                                                      >  2:484695/1‑150 (MQ=255)
            cATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAAcgcg                                                                                                                                    <  2:197345/151‑1 (MQ=255)
                  ccAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCa                                                                                                                              <  1:1032355/151‑1 (MQ=255)
                   cAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCAccc                                                                                                                               >  1:252970/1‑149 (MQ=255)
                      cGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGccc                                                                                                                          <  1:729565/151‑1 (MQ=255)
                       gATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCct                                                                                                                         >  2:916198/1‑151 (MQ=255)
                              gACCGGATGCATCGGTATTGATCATCCCACCGAGCGTGGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGGTGATCTTTTATCTCCCCTGCCTCAACGCGCACCCAGCCCGCCTCAg                                                                                                                   <  2:260746/150‑1 (MQ=255)
                                ccGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGt                                                                                                                <  2:1022896/151‑1 (MQ=255)
                                ccGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGt                                                                                                                <  1:195492/151‑1 (MQ=255)
                                    aTGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAAt                                                                                                            >  1:572517/1‑151 (MQ=255)
                                     tGCAACGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGGTCCGGCGCAAAAAAGGAGCCGAACGGTTGCAGGTACTGATTGAGTTGATGTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAAt                                                                                                            <  2:1080415/150‑1 (MQ=255)
                                      gCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAAttt                                                                                                          <  1:2986/151‑1 (MQ=255)
                                       cATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTc                                                                                                         >  2:125639/1‑151 (MQ=255)
                                              aTTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGatgatg                                                                                                  <  1:432711/151‑1 (MQ=255)
                                                 gATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCg                                                                                                <  1:430983/150‑1 (MQ=255)
                                                  atcatcCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGt                                                                                              <  1:265291/151‑1 (MQ=255)
                                                  atcatcCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGt                                                                                              >  1:21101/1‑151 (MQ=255)
                                                    catcCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTc                                                                                            <  2:621445/151‑1 (MQ=255)
                                                       cccACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCACCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCata                                                                                         <  1:1051191/151‑1 (MQ=255)
                                                       cccACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGACGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCa                                                                                           <  2:315913/149‑1 (MQ=255)
                                                         cACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATg                                                                                       >  2:356765/1‑151 (MQ=255)
                                                         cACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATg                                                                                       >  2:616779/1‑151 (MQ=255)
                                                           ccGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGc                                                                                     <  2:87521/151‑1 (MQ=255)
                                                              aGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCggg                                                                                  >  2:215084/1‑151 (MQ=255)
                                                                 gTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGAc                                                                                >  2:892606/1‑150 (MQ=255)
                                                                    gCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACatat                                                                            >  1:979304/1‑151 (MQ=255)
                                                                           tGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCATCTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCACTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATcaacaa                                                                      <  2:391459/150‑1 (MQ=255)
                                                                             cTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACaata                                                                    >  1:160721/1‑150 (MQ=255)
                                                                                gTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATcc                                                                <  1:4739/151‑1 (MQ=255)
                                                                                  cGAAAGGTGCGGCGCAAAAAAGCAGCCGAATGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCGGGGCTCAACGCGCACCCAGCCCTCTTCAGGGGTAAATTCGAGGATGCGGTTCATTTGGCGGGAAATATCAACCATAATccc                                                               >  2:311520/1‑150 (MQ=255)
                                                                                     aaGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTgg                                                           >  2:895157/1‑151 (MQ=255)
                                                                                     aaGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTgg                                                           >  1:822751/1‑151 (MQ=255)
                                                                                       gTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGtt                                                         >  1:871913/1‑151 (MQ=255)
                                                                                       gTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGtt                                                         >  1:554102/1‑151 (MQ=255)
                                                                                       gTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCGCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGtt                                                         <  2:647437/151‑1 (MQ=255)
                                                                                         tCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTgggt                                                         >  2:663136/1‑147 (MQ=255)
                                                                                         tCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGtt                                                         >  2:766772/1‑149 (MQ=255)
                                                                                          ccGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGa                                                       <  1:439902/150‑1 (MQ=255)
                                                                                          ccGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAg                                                      <  1:5185/151‑1 (MQ=255)
                                                                                                cAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCt                                                 >  1:222379/1‑150 (MQ=255)
                                                                                                  aaaaaGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTTAGCGCCTGAc                                              >  2:635100/1‑151 (MQ=255)
                                                                                                   aaaaGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGAcc                                             <  1:649348/151‑1 (MQ=255)
                                                                                                         aGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCTCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAgtg                                      >  1:115469/1‑151 (MQ=255)
                                                                                                                cGGTTTCAGGCACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGACGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAtggccgggg                                >  2:1017907/1‑149 (MQ=255)
                                                                                                                     tCAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTgccgcc                           >  1:226150/1‑151 (MQ=255)
                                                                                                                      cAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTgccgcc                           <  1:136219/150‑1 (MQ=255)
                                                                                                                      cAGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTgccgcc                           >  2:1071101/1‑150 (MQ=255)
                                                                                                                       aGGTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGGGCCGGCgccgcc                           >  2:255132/1‑149 (MQ=255)
                                                                                                                         gTACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTGccgccgccg                       <  1:17128/151‑1 (MQ=255)
                                                                                                                          tACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTGCcgccgccgc                      >  1:86507/1‑151 (MQ=255)
                                                                                                                          tACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTGCcgccgccgc                      >  1:569323/1‑151 (MQ=255)
                                                                                                                          tACTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGCGGcgccgccgc                      >  2:10593/1‑151 (MQ=255)
                                                                                                                           aCTGATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTGCcgccgccgc                      <  1:999534/150‑1 (MQ=255)
                                                                                                                              gATTGAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCCCCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTGCCGCCGCcgcg                     <  2:324731/148‑1 (MQ=255)
                                                                                                                                  gAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTGCCGCCGCCGCGGGGGGt               >  1:540441/1‑150 (MQ=255)
                                                                                                                                  gAGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCGGGTTGAGCGCCTGACCGTTAGTGCCGGTGCCGCCGCCGCGGGGGGt               <  2:234441/150‑1 (MQ=255)
                                                                                                                                   aGTTGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTGCCGCCGCCGCGGGGGGTaa             <  2:487314/151‑1 (MQ=255)
                                                                                                                                     ttGATCTTTTATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTGCCGCCGCCGCGGGGGGTaa             >  2:360445/1‑149 (MQ=255)
                                                                                                                                            tttATCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTGCCGCCGCCGCGGGGGGTAAAGATc        <  1:161661/147‑1 (MQ=255)
                                                                                                                                               aTCTCCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTGCCGCCGCCGCGGGGGGTAAAGATCAGCGAt  <  1:477374/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                  
CTGACGTTTTGCCATAGACCAGCGATCCCTGACCGGATGCATCGGTATTGATCATCCCACCGAGCGTTGCCCGGTTGCTGGTCGAAAGTTCCGGCGCAAAAAAGTAGCCGAACGGTTTCAGGTACTGATTGAGTTGATCTTTTATCACCCCGGCCTCAACGCGCACCCAGCCCTCTTCAGGGTTAATTTCGATGATGCGGTTCATATGGCGGGACATATCAACAATAATCCCCTGGTTGAGCGCCTGACCGTTAGTGCCGGTGCCGCCGCCGCGGGGGGTAAAGATCAGCGAT  >  NC_000913/1768163‑1768455

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: