Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,386,862	0	G	A	47.1%	32.8 / 52.4	51	intergenic (+31/‑72)	yfbL/yfbM	putative M28A family peptidase/DUF1877 family protein
Reads supporting (aligned to +/- strand):  ref base G (4/23);  new base A (24/0);  total (28/23)
Fisher's exact test for biased strand distribution p-value = 1.27e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGA  >  NC_000913/2386718‑2387012                                                                                                                                                 |                                                                                                                                                           ttttATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTTCCGAAAAAAAGACa                                                                                                                                                       >  2:181922/1‑150 (MQ=255)   ttATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAACGACAAGc                                                                                                                                                    >  1:486754/1‑151 (MQ=255)       cGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGca‑ca                                                                                                                                                <  1:982798/150‑1 (MQ=255)         taataaACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCA‑Cattt                                                                                                                                             >  1:185256/1‑151 (MQ=255)         taataaACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAACGACAAGcaaaatt                                                                                                                                              >  1:558239/1‑146 (MQ=255)          aataaACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAAAGACATGGA‑Catttt                                                                                                                                            >  1:736207/1‑151 (MQ=255)                cAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGCCCGAAAAAAAGAAAAGCA‑Caattttttttt                                                                                                                                      >  1:695493/1‑150 (MQ=255)                   tACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCA‑CATTTTTTTTTtcc                                                                                                                                    >  1:294572/1‑150 (MQ=255)                           gCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGCAAAAAAGAGAAGCA‑CATTTTTTTTTTCCTTATccttt                                                                                                                           >  2:1120167/1‑147 (MQ=255)                           gCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑tt                                                                                                                          <  2:1098813/151‑1 (MQ=255)                            cccGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CAGTTTATTTTCCCTCATCTG‑ttt                                                                                                                         <  1:860497/151‑1 (MQ=255)                                       cGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑Gaa                                                                                                             <  2:178036/151‑1 (MQ=255)                                        gCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGACAAGCC‑CATTTTTTTTTTCCTCATCTG‑TTTTTAGATGC‑Gaa                                                                                                             >  1:405169/1‑150 (MQ=255)                                          agacagaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGTCAGAAAAAAAGACATGCA‑CATTTTTTTTTTTCTCATCTT‑TTTTTAGATGG‑Gaaa                                                                                                            >  2:189465/1‑149 (MQ=255)                                              agaTTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGaa                                                                                                      <  1:1059641/151‑1 (MQ=255)                                                  tGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCGAAAAAAAGAAAAGCA‑CATTTTTTTTTTCCTCCTCTT‑TTTTTAAATGC‑GAAAAAAGaaaat                                                                                                   >  1:492163/1‑148 (MQ=255)                                                  tGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAAtt                                                                                                   <  2:718214/150‑1 (MQ=255)                                                    aaTTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAACGAAATGCA‑CAATTTTTTTTTCCTCATCTG‑TTTTTAGATGC‑GGAACATGAAAtt                                                                                                   >  1:381605/1‑148 (MQ=255)                                                     aTTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGAAATGCA‑AATTTTTTTTTTCCTCATCTG‑TTTTTAGAAAC‑GAAAAAAAAAAtt                                                                                                   >  2:761958/1‑147 (MQ=255)                                                                   gCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACATGCA‑CAATTTTTTTTTCCTCAACCG‑TTTTAAGAAGC‑GAAAAATAAAATTTTACAGAGGGAaaaa‑aa                                                                                 >  1:601870/1‑150 (MQ=255)                                                                   gCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGCTCCTGAAAAAAAGGCAAGAA‑CATTTTTTTTTTCCTCTCCTGCTTTTAAAAATG‑GAAA‑ACGAAAATCAAAGGAGGGAaaat‑aa                                                                                 >  2:542900/1‑150 (MQ=255)                                                                      cAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGGGCAAAAAAAACGACATAGA‑CATTTTTTTTTTTCTCCTTCG‑TTTTTATGTGC‑GAAACACGAAAATTAAAGGGGGGaaaaa‑aaa                                                                                >  1:105916/1‑148 (MQ=255)                                                                       aGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATggg                                                                            <  1:719754/151‑1 (MQ=255)                                                                       aGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGAGCCGAAAAAAAGGCAAGCA‑AAATTTTTTTTTCCTCTTCTT‑TTTTTAGAAGC‑GAAAAAAAAAAATTAACAGGGGGAaaat‑aaa                                                                                >  2:685868/1‑147 (MQ=255)                                                                        ggTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGa                                                                           <  2:771636/151‑1 (MQ=255)                                                                               gATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTg                                                                    <  2:153675/151‑1 (MQ=255)                                                                                     gtTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTAt                                                               <  2:638937/150‑1 (MQ=255)                                                                                       tataACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTAttt                                                             <  1:839639/150‑1 (MQ=255)                                                                                          aaCTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGc                                                          <  2:1054308/150‑1 (MQ=255)                                                                                            cTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCCAAAAAAACGACATTCA‑AATTTTTTTTTTCCTCCTCTG‑TTTTTACAAGC‑GAAAAAAAAAATTTAAAAGAGGAAAAAT‑AAATGGGAAAGAGTGGGTAtttt                                                            >  1:600627/1‑146 (MQ=255)                                                                                             tttGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAGCACCATTTTTTTTTCCC‑CCTCTT‑TTTTTAAATGG‑GAAAAAAGAAAATTAAAGGGGGGAAAAA‑AAAAGGGGATGAATGGGTAttttttag                                                        >  1:182952/1‑145 (MQ=255)                                                                                                  tataCAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTg                                                  <  1:803083/150‑1 (MQ=255)                                                                                                    taCAACAGTAAATAATAAAAATTTCTCGAGGCGGTCCCAAAAAAAAAAAAAAAAA‑AATTTTATTTTTTCCCATCTG‑TTTTTTGATGC‑GAAAAAAAAAAATTAAATGAGGGAAAAT‑AAATAGGGATTATTTGTCAttttttacaa                                                      >  2:81040/1‑138 (MQ=255)                                                                                                     acaacaGTAAATAATAAAAATTTCTCGAGGCGGTTCCCGAAAAAAACACAAACA‑AATTTTATTTTTTCCCCTCTG‑TTTTTAGATGC‑GGAAAATGAAAATTAAAGGGGGGAAAAT‑AAATGGGGATGATTGGGTATTTTTTAGaaa                                                     >  2:418763/1‑144 (MQ=255)                                                                                                     acaacaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACt                                               <  1:993589/150‑1 (MQ=255)                                                                                                     acaacaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTc                                              <  1:1006866/151‑1 (MQ=255)                                                                                                       aacaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGCCATGCA‑CATTTTTTTTTTCCTCATTTG‑TTTTTAGATGC‑GAAAAATGAAAATTAACGGAGGAAAAATAAAATGGGAA‑GAATGGCTATTTTTCAGaaa                                                     >  1:229408/1‑142 (MQ=255)                                                                                                        accataaaaaaataaaaTTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAg                                            <  1:142077/137‑1 (MQ=255)                                                                                                        acaGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAAAGACAAAAA‑AATTTTATTTTTTCTCACCTT‑TTTTTAGAAGCGGAAAAAAAAAATTAAACGGGGGAAAAAA‑AAATGGG‑ATGATTTGGTAttttttcgaaaat                                                   >  2:615376/1‑134 (MQ=255)                                                                                                          aGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGaaa                                         <  1:68381/151‑1 (MQ=255)                                                                                                          aGTAAATAATAAAAATTTCTCGAGGCGGGGCCAGAAAAAACGAAAAGCA‑CATTTTAATTTTCCTCCTCTG‑TTTTTAGAAGT‑GAAAAATGAAAATTTAAAGAGGAAAAAA‑AAAATGGAATGAATTGGTATTTTTCAGAAAATGACTCAGaa                                          >  2:42050/1‑150 (MQ=255)                                                                                                                  ataaAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAAc                                 <  2:1051046/151‑1 (MQ=255)                                                                                                                              cGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGa                      <  2:54085/150‑1 (MQ=255)                                                                                                                                   cGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTAc                >  1:390030/1‑151 (MQ=255)                                                                                                                                    gggTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCACTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACt               >  2:529573/1‑151 (MQ=255)                                                                                                                                        ccAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaa            <  1:181958/150‑1 (MQ=255)                                                                                                                                         cAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaa           <  2:621795/150‑1 (MQ=255)                                                                                                                                          aGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGaaaa          <  2:790167/150‑1 (MQ=255)                                                                                                                                              aaaGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCtt     >  1:535268/1‑151 (MQ=255)                                                                                                                                               aaGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCttt    <  2:182916/151‑1 (MQ=255)                                                                                                                                               aaGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCttt    >  1:1107697/1‑151 (MQ=255)                                                                                                                                                 gCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAATCCTTTGa  <  2:1130773/151‑1 (MQ=255)                                                                                                                                                 gCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGa  <  1:563363/151‑1 (MQ=255)                                                                                                                                                 |                                                                                                                                                           TTTTATCGTAATAAACAATACCACTTGCCCGGTGATACCGCAGACAGATTGAATTATCAGAAAATGGCTCAGGTAGTGGATGGTGTTATAACTTTGTTATACAACAGTAAATAATAAAAATTTCTCGAGGCGGGTCCAGAAAAAGCGACATGCA‑CATTTTATTTTCCCTCATCTG‑TTTTAAGATGC‑GAAACATGAAATTCAACGGAGGAAAAAT‑AAATGGGAATGATTGGCTATTTTGCAGAAATTGACTCAGAAAAGATTAACCAACTGCTTGAGTCTACTGAAAAACCTTTGA  >  NC_000913/2386718‑2387012

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑