Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,903,715	0	G	A	43.5%	64.0 / 50.1	62	intergenic (+341/+1032)	ygcE/queE	putative kinase/7‑carboxy‑7‑deazaguanine synthase; queosine biosynthesis
Reads supporting (aligned to +/- strand):  ref base G (9/26);  new base A (27/0);  total (36/26)
Fisher's exact test for biased strand distribution p-value = 3.45e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATACAAATA  >  NC_000913/2903569‑2903861                                                                                                                                                    |                                                                                                                                                     cGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAc                                                                                                                                                    <  1:662511/149‑1 (MQ=255)   aTGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACCCATTT‑ACCTCCAaacaaaaac‑aaa                                                                                                                                                >  1:299018/1‑150 (MQ=255)     gTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACACATTA‑GACTCAAAAAAAAAAC‑aaa                                                                                                                                                >  1:790558/1‑148 (MQ=255)     gTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTTAAAAAACTAATTT‑ACCTTaaaaaaaaaaa‑aaaa                                                                                                                                               >  2:489044/1‑148 (MQ=255)        tttAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACAAACCTTA‑AACTCAAAAAAAAAAA‑AAACTTcc                                                                                                                                           >  2:757865/1‑150 (MQ=255)           aGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACCCATTA‑AGCTCAAAAAAAAAAC‑aaaaattc                                                                                                                                           >  1:12247/1‑142 (MQ=255)               tAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAAAACTCATTA‑TTCTTCAAACAAAAAA‑AAAcattcaaaatt                                                                                                                                     >  2:297924/1‑139 (MQ=255)                aaaaGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAACCCCTCATTT‑ACCTCAAAACAaaaaa‑aaaaatccccatttttt                                                                                                                                  >  1:248400/1‑137 (MQ=255)                    gACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACtt                                                                                                                             <  1:613415/151‑1 (MQ=255)                      cAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTaa                                                                                                                           <  1:592690/151‑1 (MQ=255)                        aTACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAAcc                                                                                                                         <  1:465817/151‑1 (MQ=255)                         tACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAACACATCATTATTCCCAAAAAAAAAAAA‑AAACTTCC‑GA‑TTTTTTACttta                                                                                                                           >  2:42566/1‑146 (MQ=255)                         tACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTAAAAAAACTCACTA‑TCCTCCAAAAAAAAAC‑AAAAATCCAGA‑ATTATTATTTaaac                                                                                                                         >  1:665242/1‑148 (MQ=255)                           cGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAAt                                                                                                                      <  2:187620/151‑1 (MQ=255)                            gAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAACACACATTA‑GCCTCAAAAAAAAAAC‑AAAAATCCCAA‑TTTATTTCTTAAc                                                                                                                          >  1:609542/1‑146 (MQ=255)                                  cACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACCCATTA‑ACCTCAAAAAAAAAAA‑AAAACTTCAGA‑TTTTTTTCTTTAACaaaaaaaaa                                                                                                                >  1:287336/1‑143 (MQ=255)                                   aCTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTGTTAAAACAATCATTT‑TACTCAAAAAAAAAAC‑AAACTTTCAGA‑TTTATTTTTTTAACAAAGAATaa                                                                                                                >  2:52550/1‑149 (MQ=255)                                   aCTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACa                                                                                                              <  2:432861/151‑1 (MQ=255)                                    cTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACACAATT‑CCCTCACAAAAAAAAA‑AAAATTCCAGA‑TTTTTTATTTTAACaaataaaaaaa                                                                                                              >  2:441074/1‑141 (MQ=255)                                    cTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTTAAAACACCCATTT‑TCCTCAAAAAAAAAAA‑AAAAATCCCGA‑TTTTTTTCTTTAACaaaaaaaaaaa                                                                                                              >  1:637960/1‑141 (MQ=255)                                       tATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGc                                                                                                           <  2:665075/150‑1 (MQ=255)                                        aTCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACACCCATTT‑GCCTCAAAAAAAAAAA‑AAAAATCCCAA‑TTTTTTTCTTTACCAAAAAATAACaa                                                                                                             >  1:1130429/1‑147 (MQ=255)                                             ttttCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAAAACTCATTT‑TCCCCAAAAAAAAAAC‑AAAATTTCCAA‑TTTTTTTTTTTAACAATGAATAaaaaaaataaaa                                                                                                     >  2:946702/1‑142 (MQ=255)                                                tCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTAGTCATCAACAGCGTAGAGAGGCGGTTATGTAAAACCACTCATTA‑GCGTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAat                                                                                                  <  2:510375/150‑1 (MQ=255)                                                 cACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACACATTT‑ACCTCAAAAAAAAAAA‑AAAAATCCAGA‑TTTTTTTATTTACCAAAGAATAAAAAAATTaaaaaaaa                                                                                                 >  2:555877/1‑148 (MQ=255)                                                  aCTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTATAAAAACAATCAATATCCCCCAAAAAAAAAAC‑AAA‑TTCTTGA‑TTTTTTTTTTAACCAATGAAAAAAAAAACTaaaaaaaa                                                                                                 >  1:140680/1‑147 (MQ=255)                                                  aCTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAAACACTCATTA‑ACCTCAAAAAAAAAAA‑AAAACTTCAGA‑TTTTTTACTTTACCAATAAATAACAAACTTaaaaaaaa                                                                                                 >  1:918033/1‑147 (MQ=255)                                                      aaaGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGCTTTTTAAAAACACCCCTTA‑TCCTCCAAACAAAAACAAAACTTCAAGT‑TTTTTTTTTTAACCAAGAAAAAAAAACCTAaaaaaaaaaatgt                                                                                            >  1:503950/1‑144 (MQ=255)                                                        aGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTGTTTAAAACCACCCATCA‑GTCTCTAAAAAAAAAC‑CAACCTCCAGC‑TTTTTTACTTAACAAATAAAAAAAAAGAATAAAAAATATACttttt                                                                                         >  1:54770/1‑150 (MQ=255)                                                        aGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGCTATGTTAAAAAAAACATTT‑TACCCAAAAAAAAAAA‑AAAATTTCCGA‑TTTATTATTTTAACAAAGAATAAAAAAATTAAAAAAAATAATGtt                                                                                          >  2:116137/1‑150 (MQ=255)                                                         gTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTAt                                                                                        <  1:620306/151‑1 (MQ=255)                                                          tATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACCCCCATTA‑TCCTCAAAAAAAAAAA‑AAACATCCCGA‑ATTTTTATTTTAACAAAAAATAAAAAACTTAAAAAAAATACTGTtttt                                                                                       >  1:974812/1‑151 (MQ=255)                                                            tGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACAATCATTA‑ACCTCAAAAAAAAAAA‑AAAAATCCAAA‑TTTTTTAATTTACCAATGAATAAAAAACTTAAAAAAAATAATTTTAtttt                                                                                     >  1:3447/1‑149 (MQ=255)                                                               aCTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTaaa                                                                                  <  2:90732/151‑1 (MQ=255)                                                                  ggATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAg                                                                                 <  1:908765/149‑1 (MQ=255)                                                                    atatCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGaaaa                                                                             <  2:745405/151‑1 (MQ=255)                                                                         tttATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACAATCATTA‑TTCTCAAAAAAAAAAA‑AAAAATCCAGA‑ATTTTTACTTAAACAAAAAAAAAAAAAATTAAAAAAAAAACTTTTTTTTTTAaaaaaaaa                                                                           >  2:74794/1‑148 (MQ=255)                                                                                aTAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCa                                                                 <  1:245009/151‑1 (MQ=255)                                                                                aTAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCa                                                                 <  1:158984/151‑1 (MQ=255)                                                                                 tAAGAACTATTCATCAACAGCGTATAGAGGCGGTTTTTTAAAAACACCCATTT‑ACCTCCAAAAAAAAAC‑AAAAATTCAGATTTTTTTTTTTAACAAATAAAAAAAAACTTTAAAAAAAAAATTTTTTTTTTaaaaaaaaaa                                                                           >  1:157028/1‑141 (MQ=255)                                                                                  aaGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCAt                                                                <  2:1052371/150‑1 (MQ=255)                                                                                  aaGAACTATTCATCAACAGCGTATAGAGGCGGGTTTGTAAAAACACTCATTA‑GCCCCAAAAAAAAAAA‑AAAAATTCAGA‑TTTATTTATTTAACAATGAAAAAAAAAAATAAAAAAATAAATTTTTTTTTAAAAAAAAAATAAAAAgcatt                                                                >  1:677540/1‑146 (MQ=255)                                                                                     aaCTATTCATCAACAGCGTATAGAGGCGGTTTTTTTAAAACCCCCCTTA‑TACTCCAAAAAAAAAA‑AAAATTCCCAA‑TTTTTTACTTAAACAAAAAATAAAAAAATTTAAAAAAAAATTGTTTTTTTAGAGAAAAAAATAAAAAGCATTaa                                                             >  1:972823/1‑150 (MQ=255)                                                                                         aTTCATCAACAGCGTATAGAGGCGGTTATTTAAAAACAACCATTT‑ACCTCAAAAAAAAAAA‑AAAACTTCAGA‑TTTATTTCTTAACCAAATAAAAAAAAAATTAAAAAAAAAAATGTTTTTTTAAAAAAAAAAATAAAAAGCAATAAtt                                                           >  1:624543/1‑148 (MQ=255)                                                                                            catcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAatat                                                     <  1:757165/151‑1 (MQ=255)                                                                                            catcaACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAatat                                                     <  2:949797/151‑1 (MQ=255)                                                                                                 aCAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaa                                                <  1:314880/151‑1 (MQ=255)                                                                                                   aGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTaaaaaa                                              <  1:1129747/151‑1 (MQ=255)                                                                                                        ataGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAaataa                                          <  2:30945/150‑1 (MQ=255)                                                                                                         taGAGGCGGTTATTTAAAAACACTCATTA‑GCCCCAAAAAAAAAAA‑AAAAATCCAGA‑ATTATTACTTAAACAAAGAATAAAAAAAATAAAAAAAATAATGTTATTTTTAAGAAAAAAATAAAAAGAATTAATTCAATATTAAAAAaataa                                          >  2:502909/1‑149 (MQ=255)                                                                                                           gagGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGa                                      <  2:47406/151‑1 (MQ=255)                                                                                                            agGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGa                                      <  2:47398/150‑1 (MQ=255)                                                                                                              gCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTa                                   <  1:57990/151‑1 (MQ=255)                                                                                                               cGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTa                                   >  1:1109039/1‑150 (MQ=255)                                                                                                                   tATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAAt                               <  1:576527/150‑1 (MQ=255)                                                                                                                            ccACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACg                      >  1:281682/1‑150 (MQ=255)                                                                                                                            ccACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACg                      >  2:883674/1‑150 (MQ=255)                                                                                                                                tCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGAttt                  >  1:840992/1‑150 (MQ=255)                                                                                                                                  aTTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTa                 >  1:958519/1‑149 (MQ=255)                                                                                                                                   ttA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTa                 >  1:500326/1‑148 (MQ=255)                                                                                                                                     a‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGaa             <  2:956009/150‑1 (MQ=255)                                                                                                                                            aaaaCAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATAc       >  2:1074531/1‑150 (MQ=255)                                                                                                                                             aaaCAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATACa      >  2:184812/1‑150 (MQ=255)                                                                                                                                              aaCAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATACaa     >  2:264527/1‑150 (MQ=255)                                                                                                                                                 aaaGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATACAAATa  <  2:659198/150‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                     CGATGTCTTTAGAATAAAAGACAATACGAATCGCACTTTATCAATTTTCACTTAAAGTATGAACTGGATATCTTTATTCATAAGAACTATTCATCAACAGCGTATAGAGGCGGTTATGTAAAACCACTCATTA‑GCCTCAAAACAAAGAC‑AAACTTCCAGA‑TTTATTACTTAACCAATGAATAACAAGCTTAAAAAATATACTGTTATTCTAAAGAAAAAATTAACAGGCATTAATTCAATATTAAAAAAATAATAGATTAAAATTTCTTAACGATTTAAGAATCATACAAATA  >  NC_000913/2903569‑2903861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑