Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 73,413 | 0 | A | C | 40.4% | 105.4 / 13.2 | 58 | V370G (GTG→GGG) | thiP | thiamine/thiamine pyrophosphate ABC transporter permease |
Reads supporting (aligned to +/- strand): ref base A (30/4); new base C (0/23); total (30/28) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 3.31e-12 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
ATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTT > NC_000913/73265‑73563 | aTAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCAcc > 1:532656/1‑151 (MQ=255) gggATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGAc < 1:122318/151‑1 (MQ=255) gCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGcc < 2:661003/150‑1 (MQ=255) ccATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGcc > 1:898025/1‑149 (MQ=255) ttAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCAt > 2:919070/1‑151 (MQ=255) aaCGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCAttt > 1:1091170/1‑151 (MQ=255) aaCGCATGGGTAAAAATCACAATGCCGCCAGCAGATGGTGGCAGGCCGATAGTGTTGTTGGGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAACCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCACCCCCGGCCCCGCCAGCAtt < 2:135281/150‑1 (MQ=255) cGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCAtttt > 2:428938/1‑150 (MQ=255) gCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCt > 1:797897/1‑151 (MQ=255) cATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCt > 1:429937/1‑150 (MQ=255) ggTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCcgc > 1:558753/1‑151 (MQ=255) ggTAAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCcgc < 1:396091/151‑1 (MQ=255) aaaaTCACAATGCGGTCAGCAGATGGTGGCAGGCCGATGGTGTTGTTGAGCA‑TAAAAAAAAGCCCGGTGGCCAGCACAACCCCCGGCATGGCGGGGATCAACCTGCCGCTCATCCCCAGCCCCCGACCCGCCAGCATTTTCTGCCGCGccc < 2:1131002/151‑1 (MQ=255) aaaaCCACAAGCCCGTCAGCAGATTGTGGCGGGCCGATGGTGTTGTGGAGCAGTAAAAAGAGG‑CCGGTAGCCAGCACAACCCCCGGCATGGCGAGTATCACCATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCcgcg < 2:154431/148‑1 (MQ=255) cacaATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGt > 2:765663/1‑151 (MQ=255) aaTGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTcg > 1:793254/1‑151 (MQ=255) cGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGAc > 2:783765/1‑150 (MQ=255) cagATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTcc > 1:1058091/1‑150 (MQ=255) agATTGTGGCAGGCCGATAGTTTTGTTGAGCAGAAAAAAGAAG‑CCGGTAGCCAGCACAACCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCt < 1:136218/147‑1 (MQ=255) aTTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCAt > 1:812750/1‑149 (MQ=255) ttGTGGCGGGCCGATGGTGTTGTGGAGCAGAAAAAAGAAG‑CGGGTAGCCAGCACAATCCCCGGCTGGGCGAGGATCAACATGCCGCCCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATa < 2:132659/149‑1 (MQ=255) ttGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCAt < 1:866596/148‑1 (MQ=255) gCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGcat > 2:593740/1‑151 (MQ=255) cAGGCCGATGGGTTTGTTGAGCAGAAAAAAGAAG‑CCGTTGGCCAGCACAACCCCCGGCAGGGCGAGGATAACCAGCCCGCTAATCTCCCCCCCCGGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGcat < 2:970394/150‑1 (MQ=255) gcctatgttgttgtTGGGCAGAAAAAAGAAG‑CCGGTGGCCACCACATTCCCGGTCTTGGCGAGGATCAACATGCCGTACATCCCCAGCGCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATg < 1:122765/143‑1 (MQ=255) ccGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATgg > 1:541691/1‑151 (MQ=255) aTAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTc > 1:512743/1‑150 (MQ=255) tattgttggTGACCAGTAAAAAGAAG‑CCGGTAGCCCCCACAATCCCGGTCATGGCGAGGATCAACTTGCCGCTATTCACCAGCCCCTTACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCa < 1:231329/147‑1 (MQ=255) aGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGc > 1:918654/1‑151 (MQ=255) aGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGc > 1:1042302/1‑151 (MQ=255) gtgtTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGc > 1:245612/1‑150 (MQ=255) ttgttgAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGc < 1:566578/147‑1 (MQ=255) ttgttgAGCAGAAAAAAGAAG‑CCGGTAGCCAGCACAACCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAg < 1:417475/146‑1 (MQ=255) gCAGTAAAAAGAAG‑CCGGTAGCCAGCACAACCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGc < 1:834928/147‑1 (MQ=255) agaaaaaaGAGG‑CCGGTAGCCAGCACAACCCCCGGCAGGGCGAGGATCACCAGCCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACg < 2:670929/141‑1 (MQ=255) aGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAAt > 2:461360/1‑150 (MQ=255) aaaaaGAGG‑CCGTTGGCCGCCACAACCCCCGGCATGGCGAGGACCACCTTGCCCCTCATCCCCACCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATa < 1:301893/148‑1 (MQ=255) aaaaGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACAcc > 1:856687/1‑151 (MQ=255) aag‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTgccg > 2:269650/1‑151 (MQ=255) ggTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAgc > 1:127592/1‑151 (MQ=255) ggTAGCCACCACAACCCCGGGCAGGGCGAGGACCAACATGCCGCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAg < 2:1042267/150‑1 (MQ=255) gTACCCAGCACAACCCCCGGCAGGGCGAGGATCAACATGCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAgcg < 2:566369/151‑1 (MQ=255) ccAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAgcgc < 1:179804/148‑1 (MQ=255) caATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCa > 2:1025124/1‑150 (MQ=255) aacccccGGCATGGCGAGGATCAACATGCCGCTCACCCCCAGCACCGGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAAc < 1:681090/148‑1 (MQ=255) aaTCCCCGGAATGGGGAGGATAAACAGCCCGCCCATCCCCACCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAAc < 2:999939/151‑1 (MQ=255) ccccGGCAGGGCGAGGATCAACATGCCGCTCATCCCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACg < 1:654905/149‑1 (MQ=255) ccccGGCAGGGCAAGGACCAACATCCTGCTCATCCCCACCCCCGGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAg < 2:590129/151‑1 (MQ=255) tGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCcac > 2:660059/1‑149 (MQ=255) ggCGAGGATCAACATGCCGCTCATCCCCACCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCCGCCGCCAGCGCAATACGCAACGAGGTCcaca < 1:653407/149‑1 (MQ=255) ggCGAGAATCAACATGCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCcaca < 1:783969/149‑1 (MQ=255) cGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACACACAATACACCGGCCGCCAGCGCAATACGCAACGAGGTCCACAgcgc > 2:927758/1‑151 (MQ=255) ggATCAACATGCCGCTCATCTCCAGCCCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCt < 1:144856/150‑1 (MQ=255) cAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCcac > 1:238551/1‑151 (MQ=255) aTGCCGCTCATCCCCAGCCCCTGCCCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCa < 1:314115/151‑1 (MQ=255) gCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCAcc > 1:177460/1‑151 (MQ=255) tCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTtg > 1:430644/1‑151 (MQ=255) aTCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTtgtg > 1:71113/1‑151 (MQ=255) aCCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACtt > 1:33984/1‑151 (MQ=255) | ATAAGGGATCGCCATTAACGCATTGGTGAAAATCACAATGCCGTCAGCAGATTGTGGCAGGCCGATAGTGTTGTTGAGCAGTAAAAAGAAG‑CCGGTAGCCAGCACAATCCCCGGCATGGCGAGGATCAACATGCCGCTCATCTCCAGCACCTGACCCGCCAGCATTTTCTGCCGCGCCCGCAGTTCGCGACTGCTCCATAGCAGCATCATGGTCAGCACTACGCACAATACACCTGCCGCCAGCGCAATACGCAACGAGGTCCACAGCGCCTGCCACAGCACCGGTTGTGCCAGCACTT > NC_000913/73265‑73563 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |