Read alignment evidence... | |||||||||||
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seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
* | NC_000913 | 3,578,301 | 0 | A | C | 45.5% | 96.4 / 10.4 | 57 | G142G (GGT→GGG) | gntR | d‑gluconate inducible gluconate regulon transcriptional repressor |
Reads supporting (aligned to +/- strand): ref base A (26/4); new base C (0/25); total (27/30) | |||||||||||
Fisher's exact test for biased strand distribution p-value = 1.08e-11 | |||||||||||
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
CGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGA > NC_000913/3578153‑3578445 | cgtcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATAcc > 2:946/1‑151 (MQ=255) tcgAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCg > 1:458058/1‑151 (MQ=255) gTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTc > 1:1123100/1‑151 (MQ=255) gTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTc > 1:382915/1‑151 (MQ=255) aTAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCttt > 1:105845/1‑151 (MQ=255) ggataTGGGGCGTTCCCGGCGAAAAATAAGGGTAGTGGTCATCGGGCGTGCTCCTTCAAATTTAAAAAAC‑CC‑GCGGGGAATATCAAGGCCGGGCTTCTTGC‑TGTCCATCATTCCCACCGCGGGAATCCCCGCCACTTCAATCATCTTTaa < 2:440649/146‑1 (MQ=255) ggCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTa > 1:543830/1‑149 (MQ=255) cAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAgg > 2:432824/1‑150 (MQ=255) agtgtgGCGTCGCCGGCGAAAAAAAAGGGCAGGGGTCTTCGGCCGTCCTCCTCAAAAGTTAAAAAAC‑CC‑GCCGGCAATACCAAGCCAGGGC‑ACTTTCTTGCCCATCATTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAg < 2:616665/146‑1 (MQ=255) tgtgGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTgcg > 2:59142/1‑151 (MQ=255) ggggCGATGCCCGCGAAAAAAAGGGGAAGTGGTCATCGGGCGTGCTGCTAAAAATTTAAAAAAA‑CC‑GGCGGCGATAACAAGGCATGGCGATTTGC‑TCCCCATCAGTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCttt < 1:309207/140‑1 (MQ=255) gATCCCCGCGAGAAATAAGGGCAGTGGTCATCGGGCGTGCTGCTTAAAAGTAAAAAAAC‑CC‑GCCGGCAATATAAAGGCAGGGCGACTTGC‑TGCCCATCAGTCCCCCCGCGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCgt < 1:832057/151‑1 (MQ=255) cgcgAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGt > 1:53466/1‑151 (MQ=255) aGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGt > 1:786101/1‑151 (MQ=255) aatagtGGCGGTGTTCATCTGGGGTGCTGCTTAAAATTTATAAAAC‑CC‑GACGGCAATATCAAGGCAGGGCGACTTGT‑TGCCCATCATTCCCCCCGGGGAAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTc < 1:991707/150‑1 (MQ=255) aataagGGTGGTGTTCTTGTGGCGTGCTCCTT‑AAAATTAACAAAACCC‑GCCGGCGATACCAGGGCATGGCGTTTTTC‑TTTCCACCAGTCCCCCCGGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGt < 2:768358/148‑1 (MQ=255) aataagGGGTGTGGTCATCTGGCGTGCTTCTTAAATTTTAACAAAC‑CC‑GACGGCGATACCAAGGCATGGCTACTTGC‑TTTCCATCACTTCCCCCGCGGAAAGCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCgt < 2:1057050/138‑1 (MQ=255) ataatGGCAGTGGTCATCTGGCGTCCTGCTTAAAATTTATAAAAC‑CC‑GGGGGCGATATCAAGGCATGGCGCTTTGC‑TGTCCTTCATTCCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCgg < 1:969398/151‑1 (MQ=255) aagggCGGTGGTCATCTGGCGTGCTGCTTAAAATTTAAAAAAC‑CC‑GCCGGCGATATAAGGGCATGGCGTCTTGC‑TGTCCATCAGTCCCCCCGGGGAAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGt < 1:106091/147‑1 (MQ=255) ggCAGTGGTCATCTGGCGTGCTGCTTAAAAGTTACCAAAC‑CC‑GACGGCGATATCAAGGCGTGGCGACTTGC‑TGTCCATCAGTTCCCCCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGa < 2:721135/149‑1 (MQ=255) gCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAgg > 1:996067/1‑150 (MQ=255) cAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGAt > 1:12001/1‑151 (MQ=255) gTGGTCTTCGGGGGTGCTGCTCAAAATTTACAAAAC‑CC‑GCGGGCAATAAAAAGGCAGGGCGCTTTGT‑TGCCCATCATTCCCCCCAGGGAAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGAt < 1:187265/149‑1 (MQ=255) aTCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCg > 1:899340/1‑150 (MQ=255) gctTAAAATTTAAAAAAC‑CC‑GACGGCAATATCAAGGCTTGGCTCCTTGC‑TGTCCACCAGTCCCCCCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAg < 2:588595/150‑1 (MQ=255) cctcaaaaGTTACAAAAC‑CC‑CCCGGCGATATCAAGGCAGGGCGCTTTGT‑TCTCCATCAGTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCa < 2:276051/144‑1 (MQ=255) aaaaGTTAACAAAC‑CC‑GACGGAAATATCAAGGCATGGCGATTTGC‑TGTCCATCGGTCCCCCCAGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgag < 2:813105/148‑1 (MQ=255) aatattATAAAAC‑CC‑GCCGGCAATATCAAGGCATGGCGACTTGC‑TGTCCATCTGTCCCCCCAGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATAtt < 2:960777/137‑1 (MQ=255) aaattaataaaac‑CC‑GACGGCGATATCAGGGCGGGGCGCCTGGC‑TGTCCATCGGTCCCACCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgaga < 2:46358/140‑1 (MQ=255) aaaTTTATCAAAC‑CC‑GCCGGCGATATCAAGGCATGGCGCCTTGC‑TGTCCATCATTCCCCCCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAgg < 1:112530/146‑1 (MQ=255) aaatttaaaaacccc‑GAGGCCGATATAAAGGTAGGGCTTCTTGT‑TTCCCATCGGTCCCACCACGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAgg < 1:598724/133‑1 (MQ=255) aaatttaaaaaa‑CCGGACGGCGATATCAAGGCATGGCGCTTTCC‑TGTCCATCAGTCCCCCCCGGGAAACCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGgaga < 1:154775/141‑1 (MQ=255) aaGTTATCAAAC‑CC‑GGGGGCGATAGCACGGAAGGGCGACTTTC‑TTTCCATCATTTCCCCCAGGGAAATTCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTc < 2:869559/134‑1 (MQ=255) aGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCAt > 2:555320/1‑151 (MQ=255) tCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGAtt > 1:929025/1‑151 (MQ=255) aaac‑CC‑GACGGAGATACGAAGGCAGGGCGACTTGT‑TTTCCATCGTTCCCACCAGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTc < 2:917214/150‑1 (MQ=255) aaaa‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCg > 2:421908/1‑151 (MQ=255) aacccccacgacgATAACAAGGCATGCCCTCTTGC‑TGCCCATCATTCCCCCCGGGAGAATCACCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGAtt < 1:882779/142‑1 (MQ=255) aaccc‑gccgGCGATATCAGGGCGTGGCGTCTTGC‑TGTCCATCATTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGa < 1:944654/148‑1 (MQ=255) c‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCg > 1:893390/1‑151 (MQ=255) gATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGc > 1:134576/1‑151 (MQ=255) gATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGc > 2:84553/1‑151 (MQ=255) cAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCAt > 1:503688/1‑151 (MQ=255) ggcgtggcgtCTTGC‑TTTCCATCGTTCCCCCCGCGGAAACCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCAttt < 2:47084/150‑1 (MQ=255) gCATGGCTTCTTGC‑TCTCCATCATTCCCCCCGGGGGAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTcc < 1:749887/151‑1 (MQ=255) gccGACTTCC‑TGCCCATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGt < 1:713541/148‑1 (MQ=255) aCTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTtat > 1:80597/1‑150 (MQ=255) cTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTtata > 1:212708/1‑150 (MQ=255) ttGC‑TGTCCATCAGTTCCACCACGGGAATGCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGCTTATAAc > 2:928414/1‑151 (MQ=255) ttGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAAc > 2:958901/1‑151 (MQ=255) gTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGt > 1:636020/1‑149 (MQ=255) ccATCAGTCCCCCCGGGGAAATCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGt < 1:113312/150‑1 (MQ=255) aTCATTCCCCCCACGGGAAACCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTg < 2:884257/149‑1 (MQ=255) tCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTgcgc > 2:806864/1‑151 (MQ=255) ttCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGc > 1:872538/1‑151 (MQ=255) ggaaaTCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGt < 1:954077/144‑1 (MQ=255) ggaaaTCCCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGa < 2:254158/148‑1 (MQ=255) | CGTCGAGACGTGCGCCGAGATAGGCAATGTGGCGATGCCCGCGAGCAATAATGGCAGTGGTCATCTGGCGTGCTGCTTCAAAGTTATCAAAA‑CC‑GACGGCGATATCAAGGCATGGCGACTTGC‑TGTCCATCAGTTCCACCACGGGAATACCCGCCACTTCAATCATCTTTAAGGTGCGCGGCGTGTGGGTACGTTCGGTGAGGATCAGGCCGTCGATATTCCAGGAGAGCATGGATTCGAGGCGTTCTTGCTCCATTTCCGGTTTATAACCGTAGTGCGCCAGCATGGTCTGA > NC_000913/3578153‑3578445 |
Alignment Legend |
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Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |