Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	357,890	0	C	A	51.5%	38.8 / 103.3	69	L267F (TTG→TTT)	cynR	transcriptional activator of cyn operon; autorepressor
Reads supporting (aligned to +/- strand):  ref base C (27/6);  new base A (0/35);  total (28/41)
Fisher's exact test for biased strand distribution p-value = 1.63e-13
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.85e-09

GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCA  >  NC_000913/357742‑358034                                                                                                                                                           |                                                                                                                                                     gATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACAGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGcaaca                                                                                                                                                     >  2:694653/1‑150 (MQ=255)    aaGCGTAGCGCATCATGAAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAAc                                                                                                                                                 <  2:1141729/151‑1 (MQ=255)     aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAAcc                                                                                                                                                <  1:41165/151‑1 (MQ=255)     aGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAAcc                                                                                                                                                >  2:1009346/1‑151 (MQ=255)       cgcaGCCCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTCCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGCCTTCCAGCAATTTTT‑CCTCCCCACCAAAAccg                                                                                                                                               >  2:938793/4‑150 (MQ=17)        gTAGCGCACCAGGCAATTCCAGCCGCTGACCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTTCTCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGTCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAAccgcc                                                                                                                                             <  2:1054173/151‑1 (MQ=255)             gcATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTTCTCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCAGCGGCTGTCTGCCAGCTGTAGTT‑TCTCCGCAACACAAcggcaactc                                                                                                                                         >  2:274509/1‑145 (MQ=14)              cATCAGGCAATTCCAGCCGCTGATCTGTGTCAGCGGCTACCGTGATTCATTCCCGCCAACAACCGCGCATTTCTCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCAGCGGCTGTCTGCCAGCTATTTTT‑TCGCCGGAATACAAccagaacg                                                                                                                                          >  2:41159/1‑142 (MQ=11)               aTCAGGCAATTCCAGCCGCAGACCTGTGTTAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTtctct                                                                                                                                      >  1:632759/1‑151 (MQ=255)               aTCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTtctc                                                                                                                                       >  1:1075833/1‑150 (MQ=255)                 cAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTcc                                                                                                                                    >  1:100978/1‑151 (MQ=255)                  aGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTcc                                                                                                                                    >  1:305618/1‑150 (MQ=255)                      aaTTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCagt                                                                                                                                 >  1:478952/1‑149 (MQ=255)                          ccAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGc                                                                                                                           >  1:718399/1‑151 (MQ=255)                           cAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑g                                                                                                                         >  1:335326/1‑151 (MQ=255)                              ccGCAGACTTGTGTCAGCGGCTCCCGTGTTTCTTTCCCCCCAACACCCGCGCTTTTACCCAACGCCATTTGCAAAAAT‑C‑CC‑TT‑C‑GGGGCGGCTGTCTCCCATTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑ggg                                                                                                                       <  1:140607/150‑1 (MQ=255)                                gCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑gggg                                                                                                                      >  1:965456/1‑149 (MQ=255)                                    cccTGTGTCAGCGGCTCCCGTGATTCTTTCCCCCCACCACCCGCGCTTTTACCCACCGCCATGTGAAAAAAT‑G‑CC‑TC‑C‑GGGGCGGCTGTCTCCCAGTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAaga                                                                                                                 <  1:804919/149‑1 (MQ=255)                                      gtgtgTCAGCGCTTCCGGGGATTCTTTCCCCCCAACCCCCAAGCATTTACCAAACCCTATGACCAAAAAT‑‑‑CC‑TT‑CGGCGGCGTCTGTTTGCCGTTTTTTTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑gggg                                                                                                                      <  2:349882/142‑1 (MQ=255)                                       tgtgTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑gggg                                                                                                                      >  1:598246/1‑142 (MQ=255)                                        gtgtCGGCGGCTACCGTGATTCTTTCCCGCAAACACCCGCGCATTTACCCAACGCCATGTGAAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGTTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑aaa                                                                                                           <  1:680265/150‑1 (MQ=255)                                         tgccggCGGCTCCCGTGATTCATTCCCCCCAACACCCGCGATTTTACCCACCGCCTTGTAAAAAAAT‑C‑CC‑TT‑C‑GCGGCGGCTGTCTCCCAGTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAAt                                                                                                          <  1:702814/145‑1 (MQ=255)                                         tgccAGCGCCTCCTGTGTTTCATTCCCGCCAACACCCGCGCATTTACCCAACGCCATTTGAAAAAAT‑C‑CC‑TG‑C‑GCGGGGGTTGTCTGCCGGTTTTTTTC‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAga‑g‑a                                                                                                             <  1:399420/144‑1 (MQ=255)                                             ggcgctaccccgttttCATTCCCCCAAACACCCGC‑CATTTCTCCCACGCCTTGAGAAAAAAA‑G‑CCTTT‑C‑GCGGGGGCTGCCTGAAAGTTTTTTTT‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAg                                                                                                        <  2:576318/134‑1 (MQ=255)                                              gCGGCTCCCGTGATTCATTCCCCCCAACACCCGCTCATTTACCCACCCCCATGTAAAAAAAT‑G‑CT‑TC‑C‑GGGGGGGCTTTCGCCCATTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAAt                                                                                                          <  1:431867/145‑1 (MQ=255)                                                gcctcctgtgtTTCATTTCCGCAAACACCCGCGCATTTACCCACCGCCATGTGAAAAAAT‑G‑CC‑TC‑C‑GGGGCGTTTGTCTGCCAGCTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTaa                                                                                                  <  1:381073/144‑1 (MQ=255)                                                    aCCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGAGG‑AAAAAACTTTAAccc                                                                                               >  1:427304/1‑151 (MQ=25)                                                     ccttgtttCTTTCCCCCCAACACCCGCGCATTCCCCCACCGCCATGTGCAAAATG‑C‑CC‑TC‑C‑GGGGCGGTGGTCTGCCGGTTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGc                                                                                                <  2:1252020/142‑1 (MQ=255)                                                     ccgtatttCATTCCCCCAAAAACCCGCGCTTTTATCCACCGCCATGTAAAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCGGCCAGTTTTTTTC‑CCGCCCAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAg                                                                                                 <  1:263594/141‑1 (MQ=255)                                                     ccgtattcaaTTCCCGCCAACACCCGCCCTTTTACCCACCGCCTGGTG‑AAAAAA‑G‑CC‑TTCC‑GCGGCGGCGCTCTGCCAGTTTTTTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGccc                                                                                              <  2:174607/141‑1 (MQ=255)                                                     ccGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGA‑G‑aaaaaaactttaaacc                                                                                              >  1:461433/1‑137 (MQ=25)                                                     ccGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGACCAGA‑a‑aaagaagattcaccgc                                                                                              >  1:1027173/1‑137 (MQ=25)                                                          aTTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGCGGGGGCAGGG‑A‑AAAAAGCTTTa                                                                                                   >  2:422978/1‑141 (MQ=25)                                                             cATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGA‑G‑AAAAAACTTTaaaaccccccagtt                                                                                      >  1:715327/1‑138 (MQ=25)                                                               ttcccGCAAACACCCCCGCTTTTCCCCACCGCATTGTAAAAAAAT‑G‑CC‑TC‑C‑GCGGGGGCTTTCTCCCATTTTTTTTC‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAt‑gt                                                                                      <  1:617610/144‑1 (MQ=255)                                                                 cccccaaacaccCGCGCTTTCCCCCACCGCCATGGGAAAAAAG‑C‑CC‑TC‑C‑GGGGCGGCTGTCGGCAATTGTTTTTC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTtgtgtg                                                                                <  2:333896/145‑1 (MQ=255)                                                                 cccGCCAACACCCGCCTTTTTACCCACCCCCTTGTGAAAAAAT‑G‑CC‑TT‑C‑GCGGCGGTTGTCTGCCAGTTTTTTTC‑CCGCGGAAAAAAATCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTtgtg                                                                                  <  2:457935/148‑1 (MQ=255)                                                                  cccccAACAACCCCGTATTTACCCAACGCCATTTGCAAAAAT‑G‑CC‑TG‑C‑GCGGGGGCTTTCTGCCGGCTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTtgtgt                                                                                 <  1:1254925/146‑1 (MQ=255)                                                                        acaccccctcttttACCCACCCCCATGTAAAAAAAG‑C‑CC‑TC‑C‑GGGGGGTCTGTCTGCCTTTTTTTTTC‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATc                                                                          <  1:1089607/139‑1 (MQ=255)                                                                           acCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGA‑G‑AAAAAACTTTTATCCCCCCCT‑GTTTTGTGGGGATCg                                                                         >  1:521794/1‑148 (MQ=25)                                                                           acCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAAAAACTTTAAAACCGTCAT‑GTTGTGTGGGAATc                                                                          >  1:636108/1‑147 (MQ=255)                                                                             cgcgCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGAAGG‑G‑GAAAAAATTTATACCCGGCAT‑CTTGTGTggggagtggtgc                                                                     >  2:146945/1‑140 (MQ=25)                                                                                gcttttACCCACCGCCATGTGAAAAAAT‑G‑CC‑TC‑C‑GGGGCGGTTTTCTGCCATTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGctgctg                                                                   <  1:975438/146‑1 (MQ=255)                                                                                 cATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGAAGA‑G‑AAAAAACTTTAAAACCCCCCT‑GTTTTGTGGGAAACg                                                                         >  1:1011957/1‑142 (MQ=37)                                                                                   tttcccaacccccgtgtgAAAAATT‑C‑CC‑TG‑C‑GGGGCGGTGTTCGGCCAGTTATTTGC‑CCGCCGCAAAAAACCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGt                                                                 <  2:1231295/134‑1 (MQ=255)                                                                                   tttacccaccccattgtgAAAAAAT‑G‑CC‑TT‑C‑GGGGCGTCTGTCTGCCAGTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTa                                                                <  1:880875/135‑1 (MQ=255)                                                                                   tttATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGG‑G‑GAAAAACATTAAGCCCATCCTGGTTGTG‑GGGGATCCCTGCTgg                                                                  >  2:241619/1‑147 (MQ=25)                                                                                   tttACCCACCGCCTTGTAAAAAATT‑C‑CT‑TT‑G‑GGGGCGGCTGTCTGCCAGTTTTTTTC‑CCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTgg                                                                  <  1:458797/147‑1 (MQ=255)                                                                                   tttACCCAACTCCTGGTGAAAAAAG‑C‑CC‑TT‑G‑GGGGCGCCTGCCTGCAAGTTTTTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTaa                                                               <  1:691768/150‑1 (MQ=255)                                                                                        caagcgcttgggcaaaaaaT‑C‑CC‑TC‑C‑GCGGCGTCTTTCT‑CCGGGTGTTTTTCCCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GTGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAAc                                                              <  2:20796/132‑1 (MQ=255)                                                                                          accGCTATGTGAAAAAAT‑G‑CC‑TG‑C‑GCGGGGGCTTTCTGCCAGTTTTTTTC‑CCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAAtg                                                          <  2:13366/146‑1 (MQ=255)                                                                                          accGCCATGTGAAAAAAT‑C‑CC‑TC‑C‑GCGGCGGCTGTCTCCCATTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAAtgt                                                         <  1:366642/147‑1 (MQ=255)                                                                                            ccccATGT‑CAAAAATGG‑CC‑TG‑C‑GGGGCGGTTGTCTGCCAGTTTTTTTC‑CCCCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTgg                                                       <  1:682148/147‑1 (MQ=255)                                                                                              cccttggaaaaaaT‑GCCC‑TT‑C‑GGGCCGGCTGTCTCCCTGTTTTTTTC‑CCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTgg                                                       <  2:434569/140‑1 (MQ=255)                                                                                                 tgtgAAAAAAT‑G‑CC‑TG‑C‑GGGGGGGTTGTCGCCCAGCTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAggg                                                 <  1:725379/150‑1 (MQ=255)                                                                                                 tggcaaaaaaT‑G‑CC‑TC‑C‑GGGGCGGCTTTCTCCCATTTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAgg                                                  <  1:274649/144‑1 (MQ=255)                                                                                                  gtgCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGAAGA‑G‑AAAAAAAATTTAAACCGCCCT‑GTTGTGTGGGGATCCGTGCTggggaaaaagtg                                                        >  1:388109/1‑132 (MQ=255)                                                                                                     cAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGA‑G‑AAAAAAATTTTAAACCCTCCC‑GTTTTGTGGGAAGCGCCTCTGGGTAAAAAGTGGGAAggg                                                 >  1:339747/1‑146 (MQ=255)                                                                                                     aaaaaaT‑C‑CT‑TG‑C‑GGGGGGGCTGTCGGCTTGTTTTTTCC‑CCGCCAAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAAc                                                              <  2:536214/132‑1 (MQ=255)                                                                                                      aaaaaT‑C‑CC‑TC‑C‑GCGGCGGTTTTCTGCCAGTTTTTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTg                                            <  1:186414/150‑1 (MQ=255)                                                                                                      aaaaaG‑G‑CC‑TC‑G‑GGGGTGGCTGTTTGCGAGTTTTTTTG‑TCGCCAAAAAAAATCCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAggg                                                 <  2:1052209/145‑1 (MQ=255)                                                                                                             g‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGGAGA‑GAAAAAACCTTTAAACCC‑CCCT‑GTTTTGTGGGGATCGCTGCTGGGGACAAAGTGGGAAGGGGAgggggggg                                       >  1:540473/1‑142 (MQ=255)                                                                                                                         ggcggcTGTCTGCCAGCTATTTTC‑CCGCCACAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTcc                             <  1:1239325/151‑1 (MQ=255)                                                                                                                            ggttGTCTGCCAGTTTTTTTC‑CCGCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAg                           <  1:695138/147‑1 (MQ=255)                                                                                                                                 tctgcctctttttttc‑CCCCCGCAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGaa                         <  1:646660/136‑1 (MQ=255)                                                                                                                                 tCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCGAGA‑G‑AAAAAAATTTTAAACCCCCCT‑GTTGTGTGGGGATAGCCGCTGGGGAAAATATGGGAAAGGGAGGGGGGGGGATCAGCTCCCGaa                         >  1:972720/1‑147 (MQ=255)                                                                                                                                       gttttttttc‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAAcc                       <  1:1103892/138‑1 (MQ=255)                                                                                                                                          tATTTTC‑CCGCCGAAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAAt               <  2:466298/148‑1 (MQ=255)                                                                                                                                                t‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGGAAGAGG‑AAAAAAATTTAA‑CCCGGCAT‑GTTGTGGGGGAAAAGCTGGTGGGAAAAAAATGGGAAAGGGAGAGGGGGGGAACAACCGCACAAACCCGCTAATTGAGtttg       >  1:601971/1‑148 (MQ=255)                                                                                                                                                     ccgcAAAAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCa  <  1:393123/151‑1 (MQ=255)                                                                                                                                                           |                                                                                                                                                     GATAAGCGTAGCGCATCAGGCAATTCCAGCCGCAGACCTGTGTCAGCGGCTACCGTGATTCATTTCCGCCAACAACCGCGCATTTATCCAACGCCATGTGCAAAAAT‑G‑CC‑TT‑C‑GCGGCGGCTGTCTGCCAGCTATTTTT‑CCGCCGCAACAAAACCGCCGTTCTCTCCAGTAGTGGC‑GGGGCAAGA‑G‑AAATAGCTTTAAGCCCGTCAT‑GTTGTGTGGCAATCGCTGCTGGTAACAATGTGGAAAGGGAAGTGCGGCGAATCAGCTCCAGAACCGCGCTAATTGAGTTCGCCTCA  >  NC_000913/357742‑358034

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑