Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,966,727	C→T	P104L (CCG→CTG)	rho →	transcription termination factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,966,727	0	C	T	100.0%	289.6 / NA	76	P104L (CCG→CTG)	rho	transcription termination factor
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (33/43);  total (33/43)

TGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTC  >  NC_000913/3966580‑3966874                                                                                                                                                    |                                                                                                                                                    tGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGa                                                                                                                                                   >  2:866388/1‑150 (MQ=255)  ggAGATATTGCAGGATGTATTTGTTTTCCTCCGTTCCGCAGCCAGCTCCTCCCTCGCCGGTCCTGATGACATCTACTTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGa                                                                                                                                                   <  2:248390/149‑1 (MQ=255)  ggAGATATTGCAGGATGGTTTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGaaa                                                                                                                                                 <  1:863618/151‑1 (MQ=255)    agaTATTGCAGGATGGATTTTGATCCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAACTCCGCCGTTTCACCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGaa                                                                                                                                                  >  2:702328/1‑148 (MQ=255)    agaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGaa                                                                                                                                                  >  1:875928/1‑148 (MQ=255)        atTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTTCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAgg                                                                                                                                            <  1:729611/150‑1 (MQ=255)        atTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGt                                                                                                                                           >  2:1108521/1‑151 (MQ=255)         ttGCAGGATGGCTTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCTTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTACGATTCGCCCGCTGAAAGAAGGTg                                                                                                                                          >  2:941323/1‑151 (MQ=255)          tGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTg                                                                                                                                          >  1:996088/1‑150 (MQ=255)               gatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTCCCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCAAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCt                                                                                                                                    <  2:906477/151‑1 (MQ=255)                atggttTTGGTTTCCTCCGTTCCGCAGACAGCTCCTCCCTCGCCGGTCCTGATGACATCTACGTTCCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCt                                                                                                                                    <  2:484928/150‑1 (MQ=255)                            tcatccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCgctgct                                                                                                                       >  2:417059/4‑151 (MQ=255)                             cctcgGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGctgctg                                                                                                                      >  1:741810/1‑151 (MQ=255)                             cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCgctgct                                                                                                                       <  1:937220/150‑1 (MQ=255)                             cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCgctgct                                                                                                                       <  1:489493/150‑1 (MQ=255)                             cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGctgctg                                                                                                                      >  2:583599/1‑151 (MQ=255)                                cctttCCGCAGCCAGCTCCTACCTCGCCGCTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaa                                                                                                                    <  2:1238078/147‑1 (MQ=255)                                 cGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAg                                                                                                                  <  2:439540/151‑1 (MQ=255)                                     ccGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTa                                                                                                               >  1:1105970/1‑150 (MQ=255)                                        cAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACg                                                                                                            <  1:487154/150‑1 (MQ=255)                                          gACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGa                                                                                                           >  1:685041/1‑149 (MQ=255)                                            cAGCTCCTCCCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGtt                                                                                                       <  2:203557/151‑1 (MQ=255)                                            cAGCTCCTCCCCCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATGTTGCGCTGCTGAAAGTTAACGAAGtt                                                                                                       <  2:1260136/151‑1 (MQ=255)                                            cAGCTCCTACCTCCCCGGTCCTGATGACATCTACGTTCCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGaa                                                                                                          <  1:215958/148‑1 (MQ=255)                                             aGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTa                                                                                                      <  1:372271/151‑1 (MQ=255)                                              gCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTa                                                                                                      >  1:161826/1‑150 (MQ=255)                                                  cTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc                                                                                                 >  1:1243307/1‑151 (MQ=255)                                                          ccGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCCACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAcc                                                                                         >  2:484815/1‑151 (MQ=255)                                                          ccGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAAcc                                                                                         >  1:457976/1‑151 (MQ=255)                                                            tgTCCTGATGACATCTACGTTTCCCCTAGCCACATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                                                                                       >  2:816611/2‑151 (MQ=255)                                                            ggTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                                                                                       >  1:815282/1‑151 (MQ=255)                                                            ggTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGCGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                                                                                       >  2:1151562/1‑151 (MQ=255)                                                               ccTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaaa                                                                                    <  1:144284/151‑1 (MQ=255)                                                                cTGATGACATCGACGTTCCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTg                                                                                       <  2:193565/147‑1 (MQ=255)                                                                      aCATCTCCTTTCCCCCAACACAAATCCGCCGTTTCAACCGCCGCACTGGTGATACCATCTCTCGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGCCAAACCTGAAAACGCCCg                                                                             <  2:275099/151‑1 (MQ=255)                                                                       cATCTACTTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCg                                                                             <  2:365187/150‑1 (MQ=255)                                                                         tCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGca                                                                           >  1:179077/1‑150 (MQ=255)                                                                          cTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaac                                                                         >  1:474848/1‑151 (MQ=255)                                                                           tACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaac                                                                         >  1:1236613/1‑150 (MQ=255)                                                                            acttttCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGca                                                                           <  2:896043/144‑1 (MQ=255)                                                                                         ccAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag                                                          <  1:1227842/151‑1 (MQ=255)                                                                                          cAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAGCAAAATCCTCtgtg                                                            <  2:379395/148‑1 (MQ=255)                                                                                          cAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTg                                                            >  2:1036036/1‑148 (MQ=255)                                                                                           aaaTCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTgag                                                          <  2:843635/149‑1 (MQ=255)                                                                                            aaTCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAAc                                                       <  1:1148843/151‑1 (MQ=255)                                                                                             aTCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACt                                                      >  1:148850/1‑151 (MQ=255)                                                                                               ccgccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTa                                                    <  1:771919/151‑1 (MQ=255)                                                                                                  ccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAcc                                                 <  1:715108/151‑1 (MQ=255)                                                                                                   cgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAccc                                                <  2:1019998/151‑1 (MQ=255)                                                                                                    gTTTCAACCTCCCCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAaccc                                               >  2:51674/1‑151 (MQ=255)                                                                                                      ttCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg                                              <  2:775065/150‑1 (MQ=255)                                                                                                      ttCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCg                                              <  1:125660/150‑1 (MQ=255)                                                                                                       tCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCt                                            >  1:123880/1‑151 (MQ=255)                                                                                                        cAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCt                                            <  1:824117/150‑1 (MQ=255)                                                                                                           ccTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCAc                                        <  1:941912/151‑1 (MQ=255)                                                                                                           ccTCCGCACTGGTGATACCATCTCTGGGAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGCACTTAACCCCGCTGCa                                         >  2:719326/1‑150 (MQ=255)                                                                                                               cGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa                                    <  1:453689/151‑1 (MQ=255)                                                                                                               cGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaa                                    <  1:866940/151‑1 (MQ=255)                                                                                                                gCACTGGTGATACCATCTCTGTTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCTACAAACCTGAAACCGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCaaa                                   <  2:511023/151‑1 (MQ=255)                                                                                                                 cACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc                                  >  2:1004245/1‑151 (MQ=255)                                                                                                                 cACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc                                  >  2:1107537/1‑151 (MQ=255)                                                                                                                 cACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc                                  <  1:424296/151‑1 (MQ=255)                                                                                                                 cACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCACGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc                                  <  2:239547/151‑1 (MQ=255)                                                                                                                  aCTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAAc                                  >  1:564940/1‑150 (MQ=255)                                                                                                                     ggTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActct                               <  1:430758/150‑1 (MQ=255)                                                                                                                      gTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCg                             <  1:902575/151‑1 (MQ=255)                                                                                                                         aTACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt                          >  2:946371/1‑151 (MQ=255)                                                                                                                             cctctcTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGt                      >  2:315797/3‑151 (MQ=255)                                                                                                                                  ctGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATgg                  >  1:1164270/1‑150 (MQ=255)                                                                                                                                   tGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGa                 >  2:679441/1‑150 (MQ=255)                                                                                                                                    ggTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGaa                <  2:150083/150‑1 (MQ=255)                                                                                                                                    ggTAAGATTCGCCCGCTGAAAGAAGGTGAACGCGATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGaa                <  2:254399/150‑1 (MQ=255)                                                                                                                                     gTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGCCAAACCGAAATACGCCCGCAGCAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAATCTCTCGTCTGCGTATGGAACg              <  2:20117/151‑1 (MQ=255)                                                                                                                                     gTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACg              <  2:941433/151‑1 (MQ=255)                                                                                                                                            tCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTaa        <  1:1073093/150‑1 (MQ=255)                                                                                                                                            tCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTaa        <  1:23265/150‑1 (MQ=255)                                                                                                                                             cgcccgctGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACg      <  2:893178/151‑1 (MQ=255)                                                                                                                                               cccgctGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGt    >  2:733786/1‑151 (MQ=255)                                                                                                                                               cccgctGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGt    <  1:1192112/151‑1 (MQ=255)                                                                                                                                                ccgctgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGtt   <  1:1213632/151‑1 (MQ=255)                                                                                                                                                ccgctgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGtt   <  2:1262067/151‑1 (MQ=255)                                                                                                                                                ccgctgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGAAACGGtt   >  2:883578/1‑151 (MQ=255)                                                                                                                                                 cgctgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTc  <  1:808888/151‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TGGAGATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTC  >  NC_000913/3966580‑3966874

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑