Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,317,438	G→A	A30V (GCG→GTG)	nusA ←	transcription termination/antitermination L factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,317,438	0	G	A	100.0%	234.4 / NA	65	A30V (GCG→GTG)	nusA	transcription termination/antitermination L factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (35/30);  total (35/30)

GGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGG  >  NC_000913/3317290‑3317584                                                                                                                                                     |                                                                                                                                                   ggCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAgcacg                                                                                                                                                  >  2:833598/1‑151 (MQ=255)   cTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGTAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCt                                                                                                                                                >  2:867873/1‑151 (MQ=255)           gTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATg                                                                                                                                        <  1:636842/151‑1 (MQ=255)              aTTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCtt                                                                                                                                     >  1:192185/1‑151 (MQ=255)              aTTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCtt                                                                                                                                     <  2:12666/151‑1 (MQ=255)                ttCCTTGGTCGGCTGTGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCTCCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTc                                                                                                                                    <  2:486917/150‑1 (MQ=255)                 tCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCg                                                                                                                                   <  2:445634/150‑1 (MQ=255)                   cTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGaa                                                                                                                                 >  2:494358/1‑150 (MQ=255)                        tCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAAtctt                                                                                                                           >  1:930445/1‑151 (MQ=255)                          ggCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAAtcttct                                                                                                                         <  1:522529/151‑1 (MQ=255)                          ggCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAAtcttct                                                                                                                         >  1:406053/1‑151 (MQ=255)                           gCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTtctc                                                                                                                        >  2:725517/1‑151 (MQ=255)                             tGGGTGACTTCATCAACAACTAAACAGCGACGGAAAGAGTCAAAATAACCGCTTTTGCGATCTATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAACATCTTCTcgc                                                                                                                      >  2:94835/1‑151 (MQ=255)                                gTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTcgcg                                                                                                                     >  2:1271961/1‑149 (MQ=255)                                     ttCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAgc                                                                                                              >  1:759031/1‑151 (MQ=255)                                        atcaACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGcc                                                                                                           >  1:866091/1‑151 (MQ=255)                                           aacaacTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCttt                                                                                                        >  1:1142841/1‑151 (MQ=255)                                            acaacTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCtttt                                                                                                       >  1:371018/1‑151 (MQ=255)                                                  aaCCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTgg                                                                                                 <  1:481928/151‑1 (MQ=255)                                                    ccAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGAt                                                                                               >  2:228031/1‑151 (MQ=255)                                                     cAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGAt                                                                                               >  1:422702/1‑150 (MQ=255)                                                      aGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATAc                                                                                             >  1:611848/1‑151 (MQ=255)                                                       gCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACg                                                                                            <  1:770396/151‑1 (MQ=255)                                                        cGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACgg                                                                                           >  2:829368/1‑151 (MQ=255)                                                        cGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAAGCTTCTCGCGAGGTAGCGCCTTTTCATGGGATACgg                                                                                           <  2:411896/151‑1 (MQ=255)                                                        cGACGGAAAGTGTCAAAATCACCGCGTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACgg                                                                                           <  2:472689/151‑1 (MQ=255)                                                         gACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGc                                                                                          >  1:87240/1‑151 (MQ=255)                                                          aCGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCt                                                                                         >  2:3833/1‑151 (MQ=255)                                                            ggAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTACTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTc                                                                                       <  1:346504/151‑1 (MQ=255)                                                             gAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTc                                                                                       >  1:480512/1‑150 (MQ=255)                                                             gAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCa                                                                                      <  1:929664/151‑1 (MQ=255)                                                               aaGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAAc                                                                                    >  2:753383/1‑151 (MQ=255)                                                                   gtCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTAc                                                                                 <  1:683690/150‑1 (MQ=255)                                                                         aTCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCaaa                                                                          <  1:1272682/151‑1 (MQ=255)                                                                           cACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCaaaa                                                                         <  1:28163/150‑1 (MQ=255)                                                                                  tttGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCtt                                                                   <  1:868425/149‑1 (MQ=255)                                                                                   ttGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTg                                                                 <  1:298892/150‑1 (MQ=255)                                                                                    tGCGATCGATCTGTACGCGGACGTCGATCGCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCTCCTGTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGt                                                                <  2:651544/150‑1 (MQ=255)                                                                                      cgatcgatCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTc                                                              <  2:837164/150‑1 (MQ=255)                                                                                           gatcTGTACGCGGACGTCGACCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTCTCCAATGCTTCGATGCTCTTCTCGCGGGGCAGCGCCTTTCCGTTGGATACGCCTTCAACTACGCCCAAAATTTTTTTGTTCATcg                                                          >  2:952681/1‑149 (MQ=255)                                                                                               tGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCt                                                    >  2:218747/1‑151 (MQ=255)                                                                                                gTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCtt                                                   <  1:3020/151‑1 (MQ=255)                                                                                                   cgcgGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTc                                                >  1:617586/1‑151 (MQ=255)                                                                                                   cgcgGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTc                                                >  2:215049/1‑151 (MQ=255)                                                                                                    gcgGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCa                                               <  1:326918/151‑1 (MQ=255)                                                                                                        aCGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTc                                           >  1:158894/1‑151 (MQ=255)                                                                                                        aCGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTc                                           >  1:1087832/1‑151 (MQ=255)                                                                                                          gTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCAt                                         <  1:334775/151‑1 (MQ=255)                                                                                                                    tGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACt                                  <  2:138990/148‑1 (MQ=255)                                                                                                                       tCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGTATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTaa                             <  2:780047/150‑1 (MQ=255)                                                                                                                        cATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTaaa                            >  2:1228427/1‑150 (MQ=255)                                                                                                                         atatTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAg                          <  1:375562/151‑1 (MQ=255)                                                                                                                           atTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTg                        <  2:1190032/151‑1 (MQ=255)                                                                                                                                    ttgttgCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAATATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGTGAACCa                <  2:45032/150‑1 (MQ=255)                                                                                                                                     tgttgCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAgg              >  1:1010535/1‑151 (MQ=255)                                                                                                                                     tattgCTGTAACCAGCACGCTTTCCAATGCTTCGACAATCTTCTCGCGAGCTAGCGCAATTTCATTGGATACGGCTTCAACTACAGCCAACATTTCTTTGTTCATCGCGGGCATTTCACCTCATACAGACTCTTAAAAGTGGGGAAcccg               >  2:1161992/3‑148 (MQ=255)                                                                                                                                      gttgCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAgg              <  2:205444/150‑1 (MQ=255)                                                                                                                                      gttgCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAgg              <  2:1258471/150‑1 (MQ=255)                                                                                                                                            gTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTTAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGTACCAGGTTCGCCt       >  2:22080/1‑151 (MQ=255)                                                                                                                                             tcaccAGCACGCTTTCCAATGCTTCGAAAATCTTCTCTCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCTGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGcc        >  2:774345/4‑149 (MQ=255)                                                                                                                                               gCCAGCACGCTTTCCAATGCTTCGAAACTCTTCTCGCGAGGTAGCGCCTTTTCATTGGACACGGCTTCAACTACAGCCAAAATTTCTCTGTTCATCGCGAGCTTTTCACCTCATCCAGACTATTAAATGCGTGGAACCAGGTTCGCCTTc     >  2:516865/1‑150 (MQ=255)                                                                                                                                               gCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCt    >  1:429578/1‑151 (MQ=255)                                                                                                                                                ccAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTg   >  1:1084003/1‑151 (MQ=255)                                                                                                                                                 cAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCTTTCTgg  <  2:790780/151‑1 (MQ=255)                                                                                                                                                 cAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTgg  <  2:192112/151‑1 (MQ=255)                                                                                                                                                 cAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTgg  >  1:230815/1‑151 (MQ=255)                                                                                                                                                     |                                                                                                                                                   GGCTTCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTGG  >  NC_000913/3317290‑3317584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑