Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,530,902 A→G V513A (GTC→GCC)  yhgE ← DUF4153 family putative inner membrane protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,530,9020AG97.7% 318.6 / ‑3.8 86V513A (GTC→GCC) yhgEDUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (1/1);  new base G (45/39);  total (46/40)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGACTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAGAATGTCGCATCAG  >  NC_000913/3530755‑3531049
                                                                                                                                                   |                                                                                                                                                   
aCAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGctc                                                                                                                                                  >  2:819037/1‑151 (MQ=255)
 cAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGctct                                                                                                                                                 >  2:992512/1‑151 (MQ=255)
   aGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGT‑AGTAATTTTTCTTTCGTTATTTCGTTCGGATGTAAACTAATATCAAGCGCGTCCCATTCTTTTCTGTCTGCGTCAAAGCCATAGACAATGGCTCTGtc                                                                                                                                              >  2:222031/1‑151 (MQ=255)
    gATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGtc                                                                                                                                              <  2:484309/151‑1 (MQ=255)
    gATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGtc                                                                                                                                              <  1:334096/151‑1 (MQ=255)
     aTCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGtca                                                                                                                                             <  1:499808/151‑1 (MQ=255)
          gcCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCAtt                                                                                                                                        <  2:723856/151‑1 (MQ=255)
            cACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTAAAAGCCATAGACAATGGCTCTGTCATCATTa                                                                                                                                       >  2:709425/1‑150 (MQ=255)
              cGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTaaaa                                                                                                                                    <  1:244727/151‑1 (MQ=255)
               gCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAATGCCATAGACAATGGCTCTGTCATCATTAAAAg                                                                                                                                   <  2:90546/151‑1 (MQ=255)
               gCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGCGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGCAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTa                                                                                                                                       >  2:585597/1‑147 (MQ=255)
                        ttCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAgg                                                                                                                           >  2:860300/1‑150 (MQ=255)
                         tCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAgg                                                                                                                           <  1:72405/149‑1 (MQ=255)
                         tCGTCCCCAGTGTTCCATCCGTCGCAGCTGTGAGTAATTATGCTTTCGTTATTTCGTTCGGAAGTACACTCATATCAAGCGCATCCCATTCTTTTCTGGCTAAGTCAAAGCCATAGACAATGGCTCTGTCATCATAAAAAGCAAACAGGa                                                                                                                          <  2:899386/150‑1 (MQ=255)
                            tCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGAtcc                                                                                                                       >  2:939557/1‑150 (MQ=255)
                             ccccAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGAtccgc                                                                                                                     >  1:649852/1‑151 (MQ=255)
                                cAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGAtccgctcc                                                                                                                  >  1:52041/1‑151 (MQ=255)
                                    tttCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTACACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAACAGCAAACAGGATCCGCTCCGCCt                                                                                                              >  2:1179714/1‑151 (MQ=255)
                                     ttCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTGCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCt                                                                                                              <  2:830689/150‑1 (MQ=255)
                                       ccATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGAc                                                                                                           >  1:609888/1‑151 (MQ=255)
                                         aTCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGAcc                                                                                                          >  1:388686/1‑150 (MQ=255)
                                             ttCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCAc                                                                                                     >  1:153986/1‑151 (MQ=255)
                                               cGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACt                                                                                                    >  2:483612/1‑150 (MQ=255)
                                                  aGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTAtt                                                                                                 >  1:274735/1‑150 (MQ=255)
                                                   gCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTc                                                                                                >  1:789876/1‑150 (MQ=255)
                                                      gtgAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGa                                                                                             <  2:757717/150‑1 (MQ=255)
                                                      gtgAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTAAg                                                                                              <  2:552128/149‑1 (MQ=255)
                                                       tgAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTc                                                                                                >  1:855066/1‑146 (MQ=255)
                                                        gAGTAATTTTTCTTTCGGTATTTCGTTCGGAAGTAAACTCAGATCATGCGCCTCTCAGTCTTTTCGGTCTGAGTCAAAGCAATAGGCAATGGCGCCTTCATCATTAAATGCCAACAGGACCCGCTCCTCCTGACCATCGCTATTCAGATc                                                                                           <  2:994392/150‑1 (MQ=255)
                                                             aTTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGACCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCCCTATTCAGATCTTGCTc                                                                                     <  2:820405/151‑1 (MQ=255)
                                                              tttttCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCg                                                                                    >  1:691928/1‑151 (MQ=255)
                                                               ttttCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCg                                                                                    <  2:835408/150‑1 (MQ=255)
                                                                tttctttcGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGa                                                                                   <  2:58059/150‑1 (MQ=255)
                                                                    tttcGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAgg                                                                              >  2:9017/1‑151 (MQ=255)
                                                                    tttcGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAgg                                                                              >  2:102729/1‑151 (MQ=255)
                                                                    tttcGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCGGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAGGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAg                                                                               <  2:19744/150‑1 (MQ=255)
                                                                     ttcGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCa                                                                                <  1:724239/148‑1 (MQ=255)
                                                                      tcGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGa                                                                             <  2:366567/150‑1 (MQ=255)
                                                                       cGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGCCCATCACTATTCAGATCTTGCTCGACCAGGAc                                                                            <  2:868195/150‑1 (MQ=255)
                                                                       cGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGAc                                                                            <  2:437484/150‑1 (MQ=255)
                                                                       cGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACg                                                                           <  1:264583/151‑1 (MQ=255)
                                                                       cGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGAGCCGCTCCGCCTGACCATCAATATTCAGATCTTGCTCGACCAGGa                                                                             <  2:699099/149‑1 (MQ=255)
                                                                        gTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGc                                                                          >  2:105103/1‑151 (MQ=255)
                                                                         ttATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGc                                                                          >  1:1011190/1‑150 (MQ=255)
                                                                          tATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGACTCTGTCATCATTAAAAGCACACAGGATCCGCTCCGCCTGACCATCACTATTCAGCTCTTGCTCGACCAGGACGc                                                                          >  2:1105256/1‑149 (MQ=255)
                                                                             ttcgttcgGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGc                                                                      <  1:222110/150‑1 (MQ=255)
                                                                                 ttcgGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCtt                                                                 >  2:1184966/1‑151 (MQ=255)
                                                                                          aaaCTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATAc                                                        >  2:143324/1‑151 (MQ=255)
                                                                                          aaaCTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATAc                                                        >  2:257071/1‑151 (MQ=255)
                                                                                           aaCTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATAc                                                        >  2:1011811/1‑150 (MQ=255)
                                                                                                atatCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGtca                                                  <  1:605871/151‑1 (MQ=255)
                                                                                                 tatCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGtcat                                                 >  1:439471/1‑151 (MQ=255)
                                                                                                 tatCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGtcat                                                 >  2:733899/1‑151 (MQ=255)
                                                                                                  atCAAGCGCGTCCCATTCTTTTCTCTCTGAGTCAGCGCCATAGACAATGGCTCTGTCATCATTAAAAGCACACAGGATCCGCTCCCCCTGACCATCACTATTCAGATCGTGCTCGACCAGGACGCAGGCGGCTTTTTCAATACAGGtcat                                                 <  2:214294/150‑1 (MQ=255)
                                                                                                   tCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGtcatc                                                >  1:811961/1‑150 (MQ=255)
                                                                                                        cgcgTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGtt                                           >  1:1159586/1‑150 (MQ=255)
                                                                                                           gTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTtat                                         >  1:605041/1‑149 (MQ=255)
                                                                                                            tCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAg                                       <  2:626128/150‑1 (MQ=255)
                                                                                                             cccATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCg                                     >  1:100777/1‑151 (MQ=255)
                                                                                                             cccATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAAAACAGGTCATCACGTTATAGc                                      <  2:826756/150‑1 (MQ=255)
                                                                                                              ccATTCTTTTCTGTCTGAGTCATAGCCATAGACAATCGTTCTGTCATCATTAAACGCAAACAAGATCCGCTCCGCCTCACCAACACTATTCAGATCTTGCTCGACCAGGACGCGGGCGTCTTTTTCAATACAGGTCATCACGTtata                                        >  2:202273/1‑147 (MQ=255)
                                                                                                              ccATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGa                                    <  2:1129594/151‑1 (MQ=255)
                                                                                                                  tCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCt                                >  1:529606/1‑151 (MQ=255)
                                                                                                                     tttCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCt                                <  1:1162848/148‑1 (MQ=255)
                                                                                                                     tttCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTgg                              >  2:147682/1‑150 (MQ=255)
                                                                                                                      ttCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACCGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGCCGTCTTTTTCAATACAGGTCATCACGTTATATCGATCCTGGAt                            >  2:831553/1‑151 (MQ=255)
                                                                                                                        ctgtctgAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGAtt                           >  1:1060636/1‑150 (MQ=255)
                                                                                                                         tgtctgAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTa                          >  1:510845/1‑150 (MQ=255)
                                                                                                                           tctgAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAgg                       >  1:371572/1‑151 (MQ=255)
                                                                                                                           tctgAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAg                        >  2:250889/1‑150 (MQ=255)
                                                                                                                            ctgAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGc                      >  1:232169/1‑151 (MQ=255)
                                                                                                                              gAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGcc                     <  2:1186002/150‑1 (MQ=255)
                                                                                                                              gAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCg                    <  1:856169/151‑1 (MQ=255)
                                                                                                                              gAGTCAAAGCCATAGACAATGACTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAgg                       <  1:534859/148‑1 (MQ=255)
                                                                                                                                gTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGAc                  >  1:478738/1‑151 (MQ=255)
                                                                                                                                   aaaGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGccttcctt               <  1:819577/151‑7 (MQ=255)
                                                                                                                                   aaaGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAg               <  1:154358/151‑1 (MQ=255)
                                                                                                                                    aaGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGa                   >  2:701618/1‑146 (MQ=255)
                                                                                                                                    aaGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAGa              <  1:1253234/151‑1 (MQ=255)
                                                                                                                                      gCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAGAAt            <  1:909237/151‑1 (MQ=255)
                                                                                                                                      gCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAGAAt            >  1:683257/1‑151 (MQ=255)
                                                                                                                                             aCAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAGAATGTCGCAt     <  1:709920/151‑1 (MQ=255)
                                                                                                                                              cAATGGCTCTGTCAGCATGAAAAGCAAACGTGATCCGCTCCGCCTGGCCATCACTATTCAGATCGTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGGCATCACGTTATATCGATCCTGTATTAAGGCCGACCAGAAGGTctgatc    <  2:222357/151‑6 (MQ=255)
                                                                                                                                                aTGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAGAATGTCGCATc    >  1:196355/1‑149 (MQ=255)
                                                                                                                                                aTGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAGAATGTCGCATCAg  <  2:606509/151‑1 (MQ=255)
                                                                                                                                                aTGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAGAATGTCGCATCAg  <  2:1105296/151‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
ACAAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGACTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAGAATGTCGCATCAG  >  NC_000913/3530755‑3531049

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑