| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 1,478,324 | 0 | T | G | 41.8% | 78.6 / 23.6 | 56 | G33G (GGT→GGG) | ynbB | putative CDP‑diglyceride synthase |
| Reads supporting (aligned to +/- strand): ref base T (8/24); new base G (23/0); total (32/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.74e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
TTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACA > NC_000913/1478183‑1478470 | ttAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGATTTA‑GGCGGGAAAACGGGGGGAgg > 1:832479/1‑146 (MQ=255) aaTCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCC‑CGGGAGAAAAAGGGGGGGGGGTACAtt > 2:551278/1‑151 (MQ=255) tGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTa < 1:839016/150‑1 (MQ=255) ggAGTGTGCTTCTTATGAGCGCTGAAATTTAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGGAAAAGGGGGGGGGGTACATTACGTATTTGt > 2:984080/1‑151 (MQ=255) gAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTa < 2:201900/151‑1 (MQ=255) gtgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCtg < 1:654784/151‑1 (MQ=255) tgCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAAC‑CGGGAGAAAAGGGGGGGAGGGTAAATTTCGTATTCGTAccctg > 1:790077/1‑148 (MQ=255) tcttATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGATACC‑GGGGAGGAAAGGGGGGGAGGGTTCATTATGTATTCTTGCCCgggggg > 1:560433/1‑151 (MQ=255) gAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCC‑GGAGGGGAAAAGGGGGGGGGGTAAATTTCATAATCGCACCCGGTggggg > 2:93327/1‑146 (MQ=255) gAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATc < 2:151654/150‑1 (MQ=255) gAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCg < 1:848615/151‑1 (MQ=255) cTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACT‑CGGGGGAAAAGGGGGGGGGGGTACATTTCGTATTCGGACCTGGGGGGGGGTCGtgct > 1:251780/1‑147 (MQ=255) tGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAAC‑GGAGAGAAAAGGGGGGGAGGGTACATTACGTATTTGTACCTGGGGGGGGATCGTCAttt > 2:603022/1‑151 (MQ=255) aaaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTCC‑GGGGAGAAAAAGGGGGGGGGTTACATTACGTATTCGTACCTGGTGGGGCGTCGTCATTTGt > 2:105876/1‑151 (MQ=255) aaaTATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCAtt < 1:564753/148‑1 (MQ=255) ataATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTAAC‑CGGGGGAAAAAGGGGGGGGGGTATATTACGTATTCGTACCTGGGGGGGGATTGTCATtttttttt > 2:935130/1‑151 (MQ=255) taATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGCGGGAAAAGGGGGGGGGGGTATATTACGTGTTCGTACCTGGTGGGGGATCGTCCTTTGtttttt > 2:852613/1‑150 (MQ=255) aaTGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAAGGGGGGGGGGTAAACTACATTTTCTTACCTGGGGGGGGGGCGGCATTTGttttt > 1:853653/1‑149 (MQ=255) aTGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGGGAAAAGGGGGGGGGGGTACATTACGTATTTGTACCCGGTGGGGGAGCGTCATTTGtttttt > 2:131011/1‑148 (MQ=255) tctcTGGCAACACTTTTCTCGTTGTTAATTTTAGCAACGCTTTTAAACCGTTTTCTGCTATGGCGGTTACCTGGTGTGAAAAGGGGG‑GAGGGTGGCTTATGTTGTTGTAACTTTTGGGGTATTGTCACTTGTTTTTTTATTGTGGttttt > 1:213140/1‑148 (MQ=255) ggCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGt < 1:321349/148‑1 (MQ=255) aaCACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTGACC‑GGAGAGGAAAAGGGGGGGGGGTGCATTACTCATTATGACCTGGTGGGGCGTCCTCCTttttttttttat > 2:848012/1‑128 (MQ=255) cacTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGGAAAAGGGGGGAGGGGATATTATGTGTATGTACCTTGGGGGGGAACGTCTTTTGTTTTTTACTGGTGGTTTTCGGTTCAc > 2:413472/1‑147 (MQ=255) tttCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGGCCCCGCTGGa < 1:857540/149‑1 (MQ=255) tCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtga < 2:347567/150‑1 (MQ=255) cgcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGTTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtgac < 2:27295/150‑1 (MQ=255) cgcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGAtgacg < 1:333464/151‑1 (MQ=255) gcgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTTAC‑GGGGGGGAAAGGGGGGGGGGGTACATTTAGTATTCGTAACTGGGGGGGGATCGGCCTTTTTTTTTTAATGGGGGTTTTAGGGCCACGCTGGGtgaag > 2:711902/1‑148 (MQ=255) cgTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGGGAGAAAAGGGGGGGGGGGTACAATACGGATTCGTACCTGGTGGGGGATCGTCATTTTTTTTTTAATTGGGATTTTAGGTCCACGCAGGGtgacg > 2:702606/1‑149 (MQ=255) ttgttAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGGGAGAAAAAGGGGGGGGGGTACATTACGTATTCGTACCTGGGGGGGGATCGTCATTTGTTTTTTAATGGGGGTTTTAGGGCCACGCTGGGtggcggt > 2:334039/1‑147 (MQ=255) ttAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGGGGGGAAAGGGGGGGAGGGTACATTACGGATTTGTACCTGGTGGGGGATCGTCATTTGTTTTTTCATGGTGGTTTCAGGGCCACGCTGGGTGACGTTGAGGtttttt > 2:146898/1‑150 (MQ=255) tAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGGGAGAAAAAGGGGGGGGGGTACATTAAGTATTCGTACCTGGTGGGGGATCGTCATTTGTTTTTTAATGGTGATTTTAGGGGCACGCTGGGTGACGTTGGCGtttttt > 2:840027/1‑149 (MQ=255) tAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGGGGGGGTACATTACGTATTCGTACCTGGGGGGGGATCGTCATTTTTTTTTTAATTGGGGTTTTTGGTCCCCCCTGGGTTACGTTTGCGgtttttt > 2:793819/1‑149 (MQ=255) cAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGGGAGAAAAAGGGGGGGGGGTACATTACGTATTTGTACCTGGTGGGGCATCGTCATTTGTTTTTTTATGGTGGTTTTAGGTCCACGCTGGGTGACGTTGACGtttttt > 2:27637/1‑146 (MQ=255) aaCGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTg < 2:611252/151‑1 (MQ=255) aTAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGtt < 1:264499/151‑1 (MQ=255) ccGTTTTCTGCTATGGCGGTTACC‑GGGGAGGAAAGGGGGGGGGGGTACATTACTTATTCGTACCTGGGGGGGGATAGTCATTTGTTTTTTAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTTTGCTGATAAGtttttt > 1:76397/1‑148 (MQ=255) gTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGc < 2:845005/151‑1 (MQ=255) tgtCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGc < 1:268886/147‑1 (MQ=255) aTGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGaa < 2:38282/151‑1 (MQ=255) ggCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAata < 1:427038/151‑1 (MQ=255) ggCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAat > 1:98858/1‑150 (MQ=255) ggTTACC‑GGAGAGAAAAGGTGGTGATGTTACATTACGTATTCTTACCTGGTGGGGCATAGTCATTTGTTTTTCAATGGTGATTTCTGGTCCACGCTTGATGACGTTGACGTTTGTTGCGCTGATAAGTTTTCTGGCTTTCAAAGAATATTg < 1:601252/151‑1 (MQ=255) ggTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTTCCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTg < 2:276544/151‑1 (MQ=255) tACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTAc > 2:716142/1‑151 (MQ=255) aCC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACg > 2:790963/1‑151 (MQ=255) cc‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGc < 1:876370/151‑1 (MQ=255) c‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCt > 2:882333/1‑151 (MQ=255) agagaAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtat > 2:272070/1‑151 (MQ=255) gagaAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtat > 1:28049/1‑150 (MQ=255) agaAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTtata < 2:142248/150‑1 (MQ=255) aaaGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTg > 2:36170/1‑151 (MQ=255) aaGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGTTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGt < 1:847612/151‑1 (MQ=255) aaGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGt < 2:832877/151‑1 (MQ=255) ggggTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTAc > 2:382954/1‑150 (MQ=255) ggggTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACa < 2:82945/151‑1 (MQ=255) | TTAATCGTTGTCGGAGTGTGCTTCTTATGAGCGCTGAAATATAATGCTGGAAAAATCTCTGGCAACACTTTTCGCGTTGTTAATTTTAGCAACGCTGATAAACCGTTTTCTGCTATGGCGGTTACC‑GGAGAGAAAAGGGGGTGAGGTTACATTACGTATTCGTACCTGGTGGGGCATCGTCATTTGTTTTTCAATGGTGATTTCAGGTCCACGCTGGATGACGTTGACGTTTTTTGCGCTGATAAGTTTTCTGGCATTGAAAGAATATTGTACGCTTATATCTGTACA > NC_000913/1478183‑1478470 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |