Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,510,391	0	T	A	41.7%	41.5 / 19.4	37	V130E (GTA→GAA)	ydcR	putative DNA‑binding transcriptional regulator and putative aminotransferase
Reads supporting (aligned to +/- strand):  ref base T (18/3);  new base A (0/15);  total (18/19)
Fisher's exact test for biased strand distribution p-value = 2.93e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTAGAGGCATT  >  NC_000913/1510243‑1510541                                                                                                                                                        |                                                                                                                                                        gTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTaa                                                                                                                                                       <  1:398648/151‑1 (MQ=255)         agaTGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAAcc                                                                                                                                              <  2:574831/151‑1 (MQ=255)             gAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCca                                                                                                                                          <  2:633905/151‑1 (MQ=255)                 cAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGc                                                                                                                                      >  1:275640/1‑151 (MQ=255)                     cGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATg                                                                                                                                  >  1:237732/1‑151 (MQ=255)                     cGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATg                                                                                                                                  >  1:580757/1‑151 (MQ=255)                                    tttttttGATTTTTGGCGGGCCGGCCGCTATCGGCCGTTGGTTCCTTGCGCCTCGGCTTTC‑CCCCCCCCGCCATTTTC‑CCCCCTCCAAA‑AACAAACCCGCCCGCTGGCGCAGGAAAGA‑AAAACCGCCACAGCGATGAGCGTGATTGaaaa                                                                                                                    <  2:766306/148‑1 (MQ=255)                                    tatTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAAc                                                                                                                   >  1:516813/1‑151 (MQ=255)                                        tttGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTAc                                                                                                               >  1:314748/1‑151 (MQ=255)                                                 ttGCGGGCCAGCCGCGACCCGTCGGTCTTCCCGTTTCCCTCGCCCTTC‑CCCGCCCCGCGCTTTTC‑CCCCCCCCAAC‑AACAAACCCCCTCGCTGGCGCAGGAAAGA‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAg                                                                                                         <  2:278150/148‑1 (MQ=255)                                                 ttGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGa                                                                                                       >  2:357820/1‑150 (MQ=255)                                                   gCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGaaa                                                                                                     >  1:319418/1‑150 (MQ=255)                                                    cAGGCCACCCGCAACCCTCCGGTCTTTCCGTTTCCCCCGGCCTTC‑CCCGCCCCGCAATTTTC‑CCCCCCCCACC‑AACAAACCCGCTCGCTGGCG‑AGGAAAACAAAAACCGCCACAGCGATGAGCGTGATTGAAATCTTACCGCCAGGAAAc                                                                                                    <  1:95207/150‑1 (MQ=255)                                                     aGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTGT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGc                                                                                                  >  1:365326/1‑151 (MQ=255)                                                             cgctATCCGCCGGTCTTTCC‑TTTCCCCCCGCCTTTCCCCCCCCCGCGCCTTTC‑CCCCCCCAAAC‑AACAAACCCGCCCCCTGGCGAAGGAAAAA‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGaaa                                                                                                     <  1:91325/140‑1 (MQ=255)                                                              gcgATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCg                                                                                         >  1:803189/1‑151 (MQ=255)                                                               cgtcccGTCGGTCGTCCCTTTTCCCCCGGCCTTC‑CCCGCCCCGCGTCTTTC‑CCCCCCCCAAC‑AACAAACCCGCTCGCGGGCGAAGGAAAAA‑AAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTg                                                                                           <  2:234029/144‑1 (MQ=255)                                                                gATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTc                                                                                       >  1:952496/1‑151 (MQ=255)                                                                                      ccgcGGCCGTC‑CCCGACCGGCGGCTTAC‑CCCCCCCCAAA‑AAAAAACCCGCCGGGGGGCGCAGGAAAGA‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTa                                                                                 <  1:333278/132‑1 (MQ=255)                                                                                             cTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTAc                                                            >  2:346387/1‑149 (MQ=255)                                                                                               tt‑CCCGCCCCGGGGCTTTTCCCCCCACCAAC‑AACAAACCCGCTCGTGGGCCCGGGAAAGA‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAg                                                          <  2:10813/150‑1 (MQ=255)                                                                                               tt‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAggg                                                        >  1:93226/1‑151 (MQ=255)                                                                                                 ccccGACCGGAGCCTTTC‑CCCCCCCCAAA‑ACCTAACCCCCGCGCGGCCGAAGTAAAAA‑AAGACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTa                                                             <  2:754061/144‑1 (MQ=255)                                                                                                  cccgccccgcCTCTTTC‑CCCCCCCAAAC‑AACAAACCCCCCCGCGGGCGAGGAAAAAA‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCa                                                      <  2:902321/151‑1 (MQ=255)                                                                                                  cccgaccc‑cgacCTTC‑CCCCCCCCAACAAACAAACCCCCTCTCGGGCGCGGGAAAGA‑AAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAggg                                                        <  2:662480/149‑1 (MQ=255)                                                                                                  cccGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGc                                                       >  1:741288/1‑150 (MQ=255)                                                                                                         cgcgtttttc‑CCCCCCCCAAC‑AACAAACCCGCCCGCGGGCGCAGGAAAAA‑AAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCAt                                               <  2:109139/145‑1 (MQ=255)                                                                                                           gcgatttccccccccccAAC‑ACCTAACCCGCTCGC‑GGGGCCGGGAAGCAAAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCAt                                               <  1:397559/141‑1 (MQ=255)                                                                                                               tttc‑CCCCCCCAAAC‑AACAAACCCGCTCGGGGGCGCAGGAAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAACCTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCa                                                <  2:323135/144‑1 (MQ=255)                                                                                                                 tccccccccccAAA‑AACAAACCCCCCCGCGGGCGGGGTAAAAA‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTtct                                           <  1:834472/145‑1 (MQ=255)                                                                                                                  t‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCtg                                       >  1:550045/1‑150 (MQ=255)                                                                                                                         tCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAAt                                >  2:330885/1‑151 (MQ=255)                                                                                                                         tCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAAt                                >  2:39920/1‑151 (MQ=255)                                                                                                                                    aaaCCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACt                      >  2:225299/1‑151 (MQ=255)                                                                                                                                      cccGCGCGCGGCCGCAGGAAGAA‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTg                     <  1:39904/149‑1 (MQ=255)                                                                                                                                       ccgcccgcGGGCGCAGAAAAGA‑AAACCCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGc                    <  2:238600/150‑1 (MQ=255)                                                                                                                                       ccGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGcc                   >  1:138150/1‑151 (MQ=255)                                                                                                                                                        tAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTAGAGGCAtt  >  2:10827/1‑151 (MQ=255)                                                                                                                                                        |                                                                                                                                                        GTCACTCGAGATGAAGCAGTCGATATCAACACTTATATTTTTGATATGTTGCAGGCCAGTCGCGATCCGTCGGTCGTTCCGTTTGCCTCGGCCTTT‑CCCGACCCGCGACTTTT‑CCCCCTCCAAC‑AACTAAACCGCTCGCTGGCGCAGGTAAGC‑AAAACCGCCACAGCGATGAGCGTGATTGAAAACTTACCGCCAGGAAACGCAGAACTGCGTCAGGCTATTGCTCGTCGCTATGCCTTACAGGGCATCACCATTTCTCCTGATGAAATTGTCATTACTGCCGGGGCGTTAGAGGCATT  >  NC_000913/1510243‑1510541

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑