Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,206,435	0	C	T	41.9%	72.4 / 43.1	63	intergenic (‑179/‑28)	ttdR/ttdA	transcriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (7/29);  new base T (25/1);  total (33/30)
Fisher's exact test for biased strand distribution p-value = 5.08e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206290‑3206584                                                                                                                                                   |                                                                                                                                                        tATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGa                                                                                                                                                     <  2:275523/150‑1 (MQ=255)  aTTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCaaa                                                                                                                                                     >  1:202133/1‑147 (MQ=255)       gCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTTACACaaaaa                                                                                                                                               >  1:182277/1‑148 (MQ=255)         aaaaCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACaa                                                                                                                                            <  2:905633/151‑1 (MQ=255)            aCATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACaaaaa                                                                                                                                         <  1:388981/151‑1 (MQ=255)             cATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTAGAAAAATACaaaaa                                                                                                                                         >  1:178455/1‑150 (MQ=255)                   cATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGTTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTGCATACCGATAaaaaaagat                                                                                                                                      >  2:110495/1‑144 (MQ=255)                             tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCTCACAAAAAAAAAAAAATGAG‑GTGCCat                                                                                                                           >  2:550507/1‑147 (MQ=255)                             tctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTCAGACAAAAACAAAAAATGGAG‑GTGCCatgat                                                                                                                        >  2:33739/1‑150 (MQ=255)                              ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATATTTTCAAACAAAAACAAAAAATGGAG‑GTGTCat                                                                                                                           >  1:365254/1‑146 (MQ=255)                                ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCACAGAAAAAAAAAAAAAGGAG‑ATGccct                                                                                                                           >  2:546276/1‑142 (MQ=255)                                ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATGTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGc                                                                                                                    <  1:621465/151‑1 (MQ=255)                                    cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGaaa                                                                                                                <  2:26550/151‑1 (MQ=255)                                     aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAg                                                                                                               <  2:799418/151‑1 (MQ=255)                                        attcattcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGta                                                                                                             <  1:76803/150‑1 (MQ=255)                                            attcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGAG‑AATTTTTTCAGACAAAAACAAAAAAAGGAG‑GTGCCCTGGTGAACGAAAGtaa                                                                                                            >  1:1013170/1‑147 (MQ=255)                                             ttcTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAAAAAAAACAAAAACTGGAG‑TTTCCatgatgggaggaaggaa                                                                                                            >  1:384611/1‑135 (MQ=255)                                                 tATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCaa                                                                                                     <  1:1006192/149‑1 (MQ=255)                                                  aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAg                                                                                                  <  2:347799/151‑1 (MQ=255)                                                  aTTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAg                                                                                                  <  1:996717/151‑1 (MQ=255)                                                      tCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCa                                                                                               <  1:444568/150‑1 (MQ=255)                                                      tCGCGCTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAACAATATAAAACCACATCTTTAGTGGTAGTTTGTGGCGGTGCACATTTTTCA‑ACAAAAAAAAAAAATGGAA‑CTGCCATTTTGTGCGCAACTAGCAAACAACACGCat                                                                                              >  1:941533/1‑150 (MQ=255)                                                       cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAg                                                                                              <  1:29333/150‑1 (MQ=255)                                                       cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAg                                                                                              <  2:326662/150‑1 (MQ=255)                                                       cgcgTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGt                                                                                             <  1:874081/151‑1 (MQ=255)                                                            tGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTCCAAAAAAATAAAAAAAATGGAG‑TTGGCATGATGAGGGAAAATAATAAACaaaacaga                                                                                               >  1:613630/1‑138 (MQ=255)                                                             gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑GATTTTTTCAGACAAAAAAAAAAAATGGAG‑TTTGCATGATGAGGGGAAGTAATAAGCAACAGGCAGGGAATa                                                                                        >  1:1004591/1‑150 (MQ=255)                                                              aTTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAg                                                                                      <  2:365461/151‑1 (MQ=255)                                                                tCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTTATAAAAAAACAAAAAATGGAG‑GTGGTATGATGAGGGAAAATAATAAACAAAAAGCAGTGaaaa                                                                                        >  1:839895/1‑145 (MQ=255)                                                                      aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑aga                                                                             <  2:843208/151‑1 (MQ=255)                                                                       cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAG‑TTGCCATGATGAGCGAAAGGAATAAAAAAAAGGCAGTGAATAAGTTGaaa                                                                                >  2:383593/1‑146 (MQ=255)                                                                       cTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑aga                                                                             <  1:789975/150‑1 (MQ=255)                                                                         gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATACAAAAAATGGAG‑GTGGCCTGATGAGCGGAAGGAATAAACAACAGGCAGTGGATAAATTGAC‑aga                                                                             >  2:661525/1‑148 (MQ=255)                                                                         gTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑Agaga                                                                           <  2:796522/150‑1 (MQ=255)                                                                            aaTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGCG‑AAATTTTTCCCACAAATAAAAAAAATTGGG‑GTGTCATTATGAGGGAAAGAAAAAAGCAAAAAGCAGAGAACAAATTGAC‑AGAGAtt                                                                         >  2:753211/1‑149 (MQ=255)                                                                             aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTc                                                                      <  2:1013223/151‑1 (MQ=255)                                                                             aTTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AAAATTTTTAAAAAAAAACAAAAAACGGAGTTTGCAAGGAT‑AGCGAAAAGAATAAACAACAAGCAGGGAAAAAATTGaaaaag                                                                             >  1:854455/1‑140 (MQ=255)                                                                                aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATGGAG‑GTTCCATGATGGGCGGAAGGAAAAAACAAAAAGCAGGGAAAAAATTGAC‑AGAGAATGttg                                                                     >  2:370378/1‑147 (MQ=255)                                                                                aCATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTCGc                                                                    <  2:397352/150‑1 (MQ=255)                                                                                          ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAAAAAAAAACAAAAAATGGAG‑TTGCCATGATGAGCGAAAGTAAAAAACAAAAAGCAGTGAAAAAGTTGAC‑AGAGATTTTTGCTAAATTTTcc                                                          >  2:511995/1‑150 (MQ=255)                                                                                          ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTCGCTAACTTTACCg                                                         <  1:839733/151‑1 (MQ=255)                                                                                            tGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCTGACAAATAAAAAAAATTGGG‑GTGCCCTGATGAGGGGAAATTATAAACAAAAGGCAGGGAAAAAGTTTTA‑AGAGAATGTCGCTTACt                                                               >  2:178112/1‑143 (MQ=255)                                                                                                aaaaaaTTTAAACCCACATTTTTGGTGATATTTTGTGGAGGTG‑AATTTTCCCAGACAAATAAAAAATCTGGGG‑TCGCCATGATGCGGGAAAGTAAGAAGCAAGCGGCAGTGAATAAGTTGAC‑AGAGTTTTTCGCTAACTTTACCGCCATGa                                                   <  1:460818/151‑1 (MQ=255)                                                                                                aaaaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAATAAAAAAACTGGAG‑TTGTCATGATTAGCGAAAGTAATAAAAAACAGGCAGTGAATAAGTTGAC‑AGAGATTTTCGCTAACTTTACCGcc                                                       >  2:611529/1‑147 (MQ=255)                                                                                                  aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAACAAAAAATTGAG‑TTGGCATGATGAGCGGAAGGAATAAACAACAAGCAGTGAATAAGATGAC‑AGAGATTGTCGCTAACTTTACCCCCCTGAtt                                                 >  2:328144/1‑151 (MQ=255)                                                                                                  aaaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTCGCTAACTTTACCGCCATGAtt                                                 <  2:952911/151‑1 (MQ=255)                                                                                                   aaaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTTCAGACAAAAAAAAAAAATTGAG‑TTGGCCTGATGGGCGAAAGGAATAAACAAAAGGCAGGGAAAAAGGTGAC‑AGAGATTGTCGCTAACTTTACCGCCATGAttt                                                >  2:670587/1‑151 (MQ=255)                                                                                                           aaCCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCAATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGa                                        <  2:714331/151‑1 (MQ=255)                                                                                                             ccACATTTTTAGTGGTAGTTTGTGGCGGTA‑ATTTTTTCCAGAAAAATAAAAAAACGGGAG‑TTGCCATGATGAGCGAAAGTAAAAACCAACAGGCAGTGAAAAAGTTGAC‑AGAAATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAAt                                      <  2:626314/151‑1 (MQ=255)                                                                                                                 aTTTTTAGTGGTAGTTTGTGGCGGTG‑AAATTTTTTCGGCAAAAATAAAAAATGGGG‑GTTGCATGGTGAGGGGAAGGAAAAAAAAAAAAGCAGAGAAAAAAATGAA‑AGAGAATGTCGCTTACTTTTACCCCATGAttttt                                              >  1:841319/1‑137 (MQ=255)                                                                                                                 aTTTTTAGTGGTAGTTTGTGGCGGCG‑AGTTTTTTTCGGCAAAAACAAAAAATGGAG‑TTGGCCTGGTGGGGGAAAGGAAATAAAAAAAGGCAGGGAAAAAATTGAA‑AGAGAATGTTGGTAACTTTACCGCCATGATTTTTACCCGaa                                       >  1:240918/1‑146 (MQ=255)                                                                                                                         tGGTAGTTTGTGGCGGTG‑AATTTTTTCCGACAAAAAAAAAAAATGGAG‑GTGCCATGATGAGGGAAAGTAATAAGAAACAGGCAGGGGATAAGTTGAC‑AGAGATTGTCGCTAACTTTACCGCCATGATTTTTACCCCAATGCCTGATGACg                           >  2:829091/1‑150 (MQ=255)                                                                                                                               tttGTGGGGGTG‑AATTTTCCAAGAAAAATAAAAAACCTGGAG‑TTGCCATGATGAGCAAAAGAAATAAGCAACAGGCGGTGAATAAGTGAAC‑AGAGATTGTCGTTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGtggtgg                     <  1:60723/150‑1 (MQ=255)                                                                                                                               tttGTGACGGCA‑AATTTTTCCACACAAATACAAAACCGGGAG‑TTGCCATCATGAGCGAAAGTAATAAGAAACAGAGAGTCAATAA‑TTGCCAAAATTTTAACAATACCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggtg                      <  2:813688/149‑1 (MQ=255)                                                                                                                                       ggTG‑AATTTTTCAAGACAAATAAAAAAACTGGAT‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGCCAGTGAATAAGTTGAC‑AGAGATTGTCGCTACTTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaa            <  2:877351/151‑1 (MQ=255)                                                                                                                                          g‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAAc         >  1:736104/1‑151 (MQ=255)                                                                                                                                              tttttCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc      >  2:87738/1‑151 (MQ=255)                                                                                                                                               ttttCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTCGCTAATTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGc      <  2:669181/150‑1 (MQ=255)                                                                                                                                               ttttCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt     >  1:745694/1‑151 (MQ=255)                                                                                                                                                tttCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCt     >  1:585633/1‑150 (MQ=255)                                                                                                                                                 ttCCAGAAAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGAAACGGGCAGTGAATAATTTGAC‑AGAGATTGTCGTAATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTa    <  1:383382/150‑1 (MQ=255)                                                                                                                                                  tCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa   >  2:330797/1‑150 (MQ=255)                                                                                                                                                  tCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaa   >  2:1001568/1‑150 (MQ=255)                                                                                                                                                   ccAGACAAATAAAAAAACTGGAT‑TGGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGAC‑GGAGATTGTCGCTATATTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTaaa  <  1:180966/150‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                        TATTTCGCAAAACATTGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTG‑AATTTTTCCAGACAAATACAAAAACTGGAG‑TTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGAC‑AGAGATTGTCGCTAACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAA  >  NC_000913/3206290‑3206584

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑