Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,270,455	0	G	T	39.1%	62.7 / 19.2	46	intergenic (+853/+170)	yhaC/garK	pentapetide repeats‑related protein/glycerate kinase I
Reads supporting (aligned to +/- strand):  ref base G (5/23);  new base T (18/0);  total (23/23)
Fisher's exact test for biased strand distribution p-value = 2.39e-08
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

GTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCG  >  NC_000913/3270310‑3270596                                                                                                                                                  |                                                                                                                                               gTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGctctc                                                                                                                                           <  2:476083/151‑1 (MQ=255)    cGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGctctct                                                                                                                                          <  1:244145/149‑1 (MQ=255)       gCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCtttt                                                                                                                                       >  2:956812/1‑149 (MQ=255)           tACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGGTTTGTCTCTCtttccc                                                                                                                                   >  1:227011/1‑145 (MQ=255)            aCCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTgg                                                                                                                                <  2:563997/151‑1 (MQ=255)             ccTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGt                                                                                                                               <  1:981607/151‑1 (MQ=255)                tATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCACTgg                                                                                                                                >  2:164263/1‑147 (MQ=255)                 aTGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGt                                                                                                                            <  1:973067/150‑1 (MQ=255)                 aTGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTg                                                                                                                           <  2:826001/151‑1 (MQ=255)                   gAACCCCTATTTGGCCTTGCTCCGGGAGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTTTCTTGTGCACTTGTCGTg                                                                                                                           >  1:875356/1‑149 (MQ=255)                          tATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCCCTTGGCCTGGGTTTcccc                                                                                                                  >  1:127771/1‑151 (MQ=255)                           aTTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCACTGGTCGTGGGGTTtcccc                                                                                                                 >  2:231262/1‑151 (MQ=255)                           aTTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTccc                                                                                                                   <  1:238670/149‑1 (MQ=255)                              tGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCCCCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTccccccc                                                                                                               <  1:639494/150‑1 (MQ=255)                                 ccTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGc                                                                                                           <  2:822019/151‑1 (MQ=255)                                     gCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGtt                                                                                                        <  2:909512/150‑1 (MQ=255)                                     gCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGtt                                                                                                        <  2:136069/150‑1 (MQ=255)                                                          gCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATgg                                                                                  <  2:619177/150‑1 (MQ=255)                                                               ggACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCTCTTTGCCTGGGCGTGGGGTTTCCCCCCCCGCGGTTCCTGG‑CACCCCTCCCCATGGAGccc                                                                             >  2:796325/1‑150 (MQ=255)                                                                gACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCgg                                                                           <  2:363626/151‑1 (MQ=255)                                                                  cTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGCTTTCTTCCTTTTTTACTCGGCCTGGGGTTCCCCCCCCCGCGCCACCCTG‑CCCCCCGCCCTCTTGGGccccg                                                                           >  1:802519/1‑147 (MQ=255)                                                                      tACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTGACTGGTCGGGGGGTTTCCCCCCCGGCGTTTCCCGG‑CCCCCCGCCCTATGGAGCCCCGGCttt                                                                      >  2:525428/1‑150 (MQ=255)                                                                         cAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCGGACTTtcctc                                                                  <  2:625112/151‑1 (MQ=255)                                                                          aGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCGGACTTtcctcc                                                                 <  1:467227/151‑1 (MQ=255)                                                                             cgcgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTCACTGGCCGTGGGGTTTCCCCCCCGGCGTTACCTGGCCCCCCTCCCCCTTGGGCCCCGGCTTTTccccccc                                                               >  1:69361/1‑151 (MQ=255)                                                                               cgcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTcctcccctcc                                                            <  1:76524/151‑1 (MQ=255)                                                                                gcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCg                                                           <  1:768795/151‑1 (MQ=255)                                                                                gcgGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCg                                                           <  2:266994/151‑1 (MQ=255)                                                                                  ggggcgcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGATTTCTCTCTTTTTCACCGGCGGCGGGGTTCCCCCCCACGCCGTTCCTCG‑CCCCCCGCCCCCTGTAGACCGGGCTTTCCTCCCCCCCGcc                                                         >  1:720608/4‑151 (MQ=255)                                                                                  ggTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGcc                                                         <  2:628341/151‑1 (MQ=255)                                                                                     gcgcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCACTGGGCGTGGGGTTTCCCCCCCCGCGGTTCCCGG‑CCCCCTCCCCCATGGAGCCCCGGCTTTcccccccccccccc                                                        >  2:1010173/1‑149 (MQ=255)                                                                                      cgcTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCCCTGGTCCTTGGGCTCCCCCCCCGGCGGTACCCGG‑CCCCCCGCCCCATTGGGGCCGGGCCTTCCCCCCCTcccccc                                                        >  1:821691/1‑148 (MQ=255)                                                                                           ttACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTTTTTCCCTTGGCGTGGGGTTTCCCCCCCCGCGTTTCCCCG‑CCCCCTCCCCCCTTGGGCCCCGACCTTCCTCcccccccccccgcccccc                                                >  1:771029/1‑143 (MQ=255)                                                                                            tACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTTTCTCTTTTGCACCTGTCCGGGGGTTCCCCCCCCCGCGTTATCTGG‑CCCCCCCCCCCTTGGGGCCCCGACCTTCcccccccccccccccccccccc                                               >  1:768156/1‑151 (MQ=255)                                                                                             aCCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGCACTGGGCGTGGGGTTCCCCCCCCGGCGTTACCTGG‑CACCCCGCCCTATGGAGGCCGGGCTTTCCCCCCCTCCGCCCGCCTccccc                                               >  2:257347/1‑150 (MQ=255)                                                                                                    ccTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTCCCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGga                                       <  1:407291/151‑1 (MQ=255)                                                                                                     cTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTTCTCCCTTTTGCACCCGTCGTGGGGTTCCCCCCCCGCGCCTACCTCG‑CCCCCTCCCCCTTTGCGCCCCGCCGTTCCTCCCCCCCCCCCGTCCCCCCCCaaca                                          >  1:726462/1‑145 (MQ=255)                                                                                                       ttCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGgac                                      <  2:721794/149‑1 (MQ=255)                                                                                                          aCCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTTTACTGGTCGTGGGGTTCCCCCCCCCGCGGTACCCGG‑CACCCTCCCCTATGGGGCCCGGACTTTTCTCCCCCCCCCCCGCCTCCCCCCCAGAgggc                                      >  2:898970/1‑144 (MQ=255)                                                                                                                aCCTGATCCCGCTTGCGCGGGCCATCGGCGGGTTTCCCTCTGTTGGCCTGGTCGTGGGGTTCCCCCCCCGGCGGTACCCGG‑CACCCCGCCCCATGGGGACCGGGCTTTCCTCCCCCCCCCCCCCCTCCCCCCCAGAGgacgacgac                                >  1:303679/1‑146 (MQ=255)                                                                                                                    gATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAgcggc                        <  1:358377/150‑1 (MQ=255)                                                                                                                    gATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAgcggc                        <  1:182493/150‑1 (MQ=255)                                                                                                                       cccGCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTGGCCTGGGCGGGGGGTTCCCCCCCCGGCGGTACCCGG‑GACCCCGCCCCATGGGGCCCGGGCGTTCCTCCCCCCCCCCCGTCTCCCCCCAAGAGGACGACGACGAAacggcg                       >  2:40843/1‑148 (MQ=255)                                                                                                                          gCTTGCGCGGGCCATCGGCGGTTTTCTCTCTGTTTCCCTGGTCGTGGGTTTTCCCCCCCGGCGGTACCCGG‑CACCCCGCCCCATGGGGCCCCGGCTTTTCTCCCCCCCCCCCCGCTCCCCCCCAGAGGACGACGACGGAacgg                         >  2:833570/1‑143 (MQ=255)                                                                                                                          gCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTTCACCGGTCGTGGGGTTTCCCCCCAGGCGGTACCTGG‑CACCCCGCCCCATGGAGCCCCGGCTTTCCTCCCCCCCCCCCCTCTCCCCCCAAGAGGACGACGACGAAGCGGCGACCGt                  >  2:826351/1‑150 (MQ=255)                                                                                                                                         cggcggTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTAACTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGAAGACGAAGCGGCGACTGTCTGGTCAGCTTCGgcg  >  2:621525/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                               GTACGGGCCGTACCTTATGAACCCCTATTTGGCCTTGCTCCGGGTGGAGTTTACCGTGCCACGGACTGTTACCAGCCGCGCGGTGCGCTCTTACCGCACCCTTTCACCCTTACCTGATCCCGCTTGCGCGGGCCATCGGCGGTTTGCTCTCTGTTGCACTGGTCGTGGGTTTCCCCCCCAGGCGTTACCTGG‑CACCCTGCCCTATGGAGCCCGGACTTTCCTCCCCTCCGCCCGTCTCCCCCGAAGAGGACGACGACGAAGCGGCGACTGTCTGGTCAGCTTCGGCG  >  NC_000913/3270310‑3270596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑