| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 646,747 | 0 | T | G | 46.9% | 61.8 / 17.1 | 49 | T255P (ACC→CCC) | citG | 2‑(5''‑triphosphoribosyl)‑3'‑dephosphocoenzyme‑A synthase |
| Reads supporting (aligned to +/- strand): ref base T (3/23); new base G (22/1); total (25/24) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.07e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCC > NC_000913/646602‑646897 | atTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGa < 1:612083/150‑1 (MQ=255) aaTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTCGGATTgcccc > 2:626573/1‑151 (MQ=255) aaTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGGCGCAAGccccc > 1:704133/1‑151 (MQ=255) aTTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCt < 2:576547/151‑1 (MQ=255) tttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTTGAATGccccc > 2:1024772/1‑149 (MQ=255) ttATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTTGCATACCCCCctt > 1:786161/1‑151 (MQ=255) tATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCttt < 2:267579/150‑1 (MQ=255) tATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGAACAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GGCGGGGTC‑AATGCCCCCcttt > 1:883916/1‑151 (MQ=255) gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTTGGATGCCCCCTTTTTGCaata > 1:513458/1‑150 (MQ=255) gCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTCGGATGCCCCCctttt > 2:442934/1‑144 (MQ=255) ttAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTTGAATGCCCCCCTTTTGtaataa > 2:763650/1‑149 (MQ=255) atTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTTGAATGCCCCCCCTTTGTAATAATtttt > 1:297725/1‑147 (MQ=255) aaaTCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCt < 2:443051/150‑1 (MQ=255) gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGTCGCCTGGACTGAGATTTCGTTCGATCCACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GCGGTCGAAAGCCCCCTTTTTGCAATACTGTTTGCGCCTCGCGTTg < 1:330024/151‑1 (MQ=255) gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTcgcg < 1:649636/148‑1 (MQ=255) gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCt < 1:659908/150‑1 (MQ=255) gtgCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTTGCCCCgcgc > 2:346342/1‑149 (MQ=255) gCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTCCCATGGCCCCCTTTTgtagataagttt > 1:371066/1‑125 (MQ=255) aaaaaCCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGTGCCTCGCGCTGTAGcc < 2:742416/151‑1 (MQ=255) aaCCAGGTAAGGATCAGGAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGGTGAAAGACCCCCTTTTTTAATAAATTTTGTGCCCcgccgcgcagac > 1:440009/1‑139 (MQ=255) aGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTCGCATGCCCCCCTTTTTTAATAAAATTTTTGCCCCCCGCCGTAGCCCgcgc > 2:282885/1‑149 (MQ=255) tAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGCGGG‑GGGTGGGAATGCCCCCTTTTT‑CAAAAAAGTTTTGGGCTCCCGCCGTAGGCacccccgg > 1:301596/1‑142 (MQ=255) aaGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGcc < 1:763404/150‑1 (MQ=255) cAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTc < 2:589854/150‑1 (MQ=255) cAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTc < 1:924499/150‑1 (MQ=255) cAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTc < 2:533750/150‑1 (MQ=255) aGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGc < 2:741670/151‑1 (MQ=255) aCTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTTGAAAGCCCCCCTTTTTTAATAAAGTTTTTGCCCCGCCCTGTAGCCAGCGCCGGCCCCCCCCCCCACcccgc > 2:396964/1‑148 (MQ=255) tGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTCGGATGCCCCCCTTTTTTAATAAATTTTTCGCCCCCCGCCGTAGCCCGCGCCGGcccccccccccccccccc > 2:928247/1‑134 (MQ=255) cgccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTCGAATGCCCCCCTTTTTCAATAATGTTTTTGCCCCCCGCTGTAGCCAGCGCAAGCCCCCCCCCCCCCCCCGCGCTGGaa > 2:915974/1‑150 (MQ=255) ccTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGACTGCCCCCTTTTTGTAATAAAAATTGCGCCGCGCGCCGTATCCAGCGCAGCCCCCCCCCCCCACCCCGCGCTGCCAcat > 1:1034271/1‑149 (MQ=255) gagaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATc < 2:818376/150‑1 (MQ=255) gagaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCg < 2:519552/151‑1 (MQ=255) gagaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTCGGAAGCCCCCCTTTTTCAATAATTTTTTTGCCCCGCGCCCTAGCCAGCGCAAGGCCCCCCCGCCCCCCCCCGGCGCAACGTTgg > 2:440450/1‑146 (MQ=255) agaTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCg < 1:924931/150‑1 (MQ=255) aTTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCg < 2:474997/151‑1 (MQ=255) gttcgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTCGAATGCCCCCCTTTTTCAATAAAGTTTTCGCCCCGCGCCGTAGCCCGCGCAGGCCCCCCCCCCCACCCCCCGCAGCCACGGTGGTATAGCCGCTggt > 1:114018/1‑149 (MQ=255) cgATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGc < 2:388806/151‑1 (MQ=255) acacTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCAt < 1:944225/150‑1 (MQ=255) acacTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTCGAATGCCCCCCTTTTTCAAAAATATTTTCGCCTCCCGCTGTAGCCAGCGCCGGCCCCCCCCCCCCCCCCCCGCTGCAACGTTGGGGTCGCCCGTGGTCGcc > 2:537988/1‑148 (MQ=255) cccTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAg < 1:396750/151‑1 (MQ=255) tGCCGGAGATAATCGAGATCGGC‑GG‑GGGGTTGAATGCCCCCCTTTTTTAATAATTTTTTCGCCCCCCGCTGTAGCCCGCGCAGGCCCCCCCCCCCCCCCCCCGGTGCAACGTTGGGATCCCCGTTGGTCGCCCTCCGTAGGAGGAAgg > 2:575961/1‑148 (MQ=255) gCCGGAGATAATCGAGATCGGC‑GG‑GGGGTCGAATGCCCCCTTTTTTCAATAATGTTTTCGCCCCCCGCCGTAGCCAGCGCAGGCCCCCCCCCCCCCCCCCCGCTGCAACCTTGGGATCGCCGGTGATCGCCCTCAGTAGGAGCAAGGGAt > 2:845977/1‑150 (MQ=255) gagaTAATCGAGATCGGC‑GG‑GGGGTCGAAAGCCCCCCTTTTGCAATAATTTTTTCGCCCCGCGCTGTAGCCAGCGCAGGCCCCCCCCGCCCCCCCCCCGCGCAACGCTGGTATCGCCGTTTATCGGCATCAGTAGGAGCAAGGTATCGa > 2:101500/1‑149 (MQ=255) cGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCa < 1:442702/150‑1 (MQ=255) gaTCGGC‑GG‑GGGTTCGAAAGCCCCCTTTTTGTAATAAAGTTTTCGCCTCCCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCCCGCGATGCCACGTTGGTATCGCCCTTGATCGCCATCAGTAGGAGCAAGGTCTCCAGCAATGCCCGtt > 1:796510/1‑150 (MQ=255) cggc‑gg‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAgg < 2:201558/151‑1 (MQ=255) gg‑gggTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCt < 2:303633/151‑1 (MQ=255) g‑gggTTCGAATGCCCCCTTGGTGCAACAATGTTTCCGCCTCGCGCTGTATGCGGCGCTGGCTCCCCTCGCCACCGCGCGATGCAACGTTGGTCTCGCGGTTGATCGCCCTCGGTAGGAGCTAGGTTTCGAGCAATGCCAGTTCAggtt < 1:334007/148‑3 (MQ=255) ttCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAAccc > 2:325075/1‑151 (MQ=255) | ATTTCCAAATTTATCAAGTGCTTAAATAATTAAATCTGTGCTAAAAACCAGGTAAGGATCAGTAGGTCAGCACTGCCGCCTGGACTGAGATTTCGTTCGATACACTCCCTGTCGAACTGCCGGAGATAATCGAGATCGGC‑GG‑GGGTTCGAATGCCCCCTTTTTGCAATAATGTTTGCGCCTCGCGCTGTAGCCAGCGCAGGCCCCCCTCGCCACCGCGCGATGCAACGTTGGTATCGCCGTTGATCGCCATCAGTAGGAGCAAGGTATCGAGCAATGCCAGTTCAGGATCTAACCC > NC_000913/646602‑646897 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |