Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	907,580	0	T	G	40.5%	60.4 / 16.9	42	I235L (ATC→CTC)	ybjT	putative NAD‑dependent oxidoreductase
Reads supporting (aligned to +/- strand):  ref base T (17/8);  new base G (0/17);  total (17/25)
Fisher's exact test for biased strand distribution p-value = 4.41e-06
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.68e-01

GTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTT  >  NC_000913/907436‑907728                                                                                                                                                 |                                                                                                                                                       gTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGggggatgggg‑at                                                                                                                                                 >  2:323340/1‑151 (MQ=255) gTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGggggatgggg‑at                                                                                                                                                 <  2:773089/151‑1 (MQ=255)    gtgGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCa                                                                                                                                               <  1:855577/150‑1 (MQ=255)      gggATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAAcc                                                                                                                                            <  2:285978/151‑1 (MQ=255)            aGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAgcggc                                                                                                                                      <  2:426078/151‑1 (MQ=255)                cACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑tt                                                                                                                                 <  2:951490/151‑1 (MQ=255)                        gcgGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTc                                                                                                                          >  2:406906/1‑150 (MQ=255)                             atcatcCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGcc                                                                                                                    <  1:594880/151‑1 (MQ=255)                                 tcCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGCTGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCAt                                                                                                                  >  1:80818/1‑149 (MQ=255)                                   cGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATaaa                                                                                                               >  2:614799/1‑150 (MQ=255)                                         aagttCGTGTTTCAGCCCCGAAACCAACGCCCTGGGGGGGGGGGGGGGTACGGAAGTAATCACATTGAAAACCCCCACCGAAACCCAGGGGGGGGGGAGGGGGAGGGGG‑ATAAACCGGGGGCGC‑TTCCCGCTCACCGCCATAAAATGt                                                                                                           <  1:417086/144‑1 (MQ=255)                                                tttttCACCCCTTGAATCACCGCCCTGGGGGTGGTGGGGGGTAGGAAAGTAATCACATTGAAAACCCCCCCCGAAACCCGGCGGGTGGGGGGGGGGATGGGG‑ATCACCCAGCGGCGC‑TTCCCGCTCACCGCCATAAAATGTTCAAACTg                                                                                                   <  2:855034/147‑1 (MQ=255)                                                tgtTTCACCCCCTGAATCACCGCCGGGGGGGGGGGGGGCGGTACGAAAGAAACCACATTGAAAACCCCCCCCGAAACCCGGGGGGGGGGGAGGGGGAGGGGG‑ATAAACCGGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACt                                                                                                    <  2:537029/148‑1 (MQ=255)                                                  tttCACCCCTTGAATCACGGCCGTGGCGGTGGTGGGCGGCAGGAATTTATTCAGACTGAGAACCCACACCAAAATCCGGCGGGGGGGGAGGGAGATGGGA‑ATCAGCCGGCGGCGC‑TAGCCCCTCACCGCCATAAAATGTTCAAACTGt                                                                                                  <  1:82259/148‑1 (MQ=255)                                                     cAGCCCCAGAATCAACGCCGGGGCGTGGGGGGGCGGTACGGAAGAAATCAAATTGAAAACCCCCCCCGAACCCCGGGGGGGGGGGGGGGGGAGGGGG‑ACCAGCCAGCGGCTC‑TGCCCTCTCACCGCCATAAAATGTTCAAACTGTTGCTg                                                                                             <  1:1039060/150‑1 (MQ=255)                                                       gCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGAt                                                                                           >  1:148766/1‑150 (MQ=255)                                                          ccTGAATAACCGCCCGGGGGGGGGGGGGCGGTAGGAAAGTAATCACATTGAAAAACCACCCCAAAACCCGGGGGGGGGGGGGGGGGAGGGGG‑ATAAACCGGCGGCGC‑TTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACt                                                                                       <  2:1022642/151‑1 (MQ=255)                                                          ccTGAATAAACGCCCGGGGGGGGGGGGGCGGTAGGGAAGTAATCACATTGAGAACCCACCCCAAAACCCAGCGGGGGGGGGGGGGGAGGGGA‑ATCAACAGGGGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACt                                                                                       <  2:287053/151‑1 (MQ=255)                                                              aaccAACGCCCTGGGGGGGGTGGGCGGTACGGAAGTAATCAATTTGAGAACCCCCCCCAAACTCCGGGGGGTGGGGGGGGGGAGGGGG‑ATCACCCGGCGGCGC‑TTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTg                                                                                      <  1:364933/145‑1 (MQ=255)                                                              aaacaccGCCCGGGGGGGGGTGGGGGGTACGGAAGTAATCACATTGAAAACCCACACCAAAACCCGGGGGGGGGGGGGGGGGAGGGGG‑ATCAACCAGCGGCGC‑TTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGa                                                                                     <  2:471457/143‑1 (MQ=255)                                                               aTCAACGCCCTGGCGGAGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGc                                                                                   >  1:841938/1‑150 (MQ=255)                                                                 cAACGCCCTGGCGGTGGTGGGCGGGACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCAcc                                                                                >  1:589195/1‑151 (MQ=255)                                                                          ggCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcg                                                                       >  2:839137/1‑151 (MQ=255)                                                                           gCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcgg                                                                      >  2:327363/1‑151 (MQ=255)                                                                             ggtggtGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCcggcg                                                                    >  2:756011/1‑151 (MQ=255)                                                                                    ggCGGTGGGGAAGAAATCAAATGGAAAACCCACCCAAAAAGCCGGGGGGGGGGGGGGGGGGGGGGG‑ACCACCCAGCGGCTC‑TGCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGa                                                             <  1:877883/151‑1 (MQ=255)                                                                                     gCGGTACGGAAATAATCAAATTGAAAAACCACACCAAAACCCGGGGGGGGGGGGGGGGGAGGGGG‑ATCACCCGGCGGCGT‑TTCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGaa                                                            <  2:55579/151‑1 (MQ=255)                                                                                      cGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGa                                                             >  1:466771/1‑149 (MQ=255)                                                                                        gTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGa                                                             >  2:904299/1‑147 (MQ=255)                                                                                            ggAAGAAATCACATTGAGAACCCACCCCGAAACCCGGGGGGTGGGGGGGGGGAGGGGG‑ATCAACCGGCGGCGC‑TTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGc                                                       <  2:193515/149‑1 (MQ=255)                                                                                                  aaTCAAATTAAAAACCCCCCCCGAAACCCAGGGGGTGGGGGGGGGGAGGGGG‑ATCAACCAGCGCCTC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGtt                                                 <  2:993212/149‑1 (MQ=255)                                                                                                   aTCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGc                                              >  1:381551/1‑151 (MQ=255)                                                                                                         ttGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCg                                        >  2:872665/1‑151 (MQ=255)                                                                                                          ggaaaccccACACCGAACCCCGGGGGGGGGGGGGGGGGGGGGGG‑AGCACCCGGCGGCGG‑TGACCGCTCCCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCgg                                       <  1:333639/144‑1 (MQ=255)                                                                                                           aagaAACCACCCCGAAACCCGGCGGGGGGGGAGGGGGTGGGGG‑ATCAACCGGCGGCGT‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGga                                      <  2:285361/150‑1 (MQ=255)                                                                                                            aaaacccccccagAAATCCAGGGGGGGGGGGGGGGGAGGGGA‑ATCAACCAGC‑GCGCTTTCCCGCTCGCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGcc                                         <  1:1003562/135‑1 (MQ=255)                                                                                                                  cacaCCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCt                                >  2:444802/1‑150 (MQ=255)                                                                                                                       cGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTaacaac                          >  2:872515/1‑151 (MQ=255)                                                                                                                         aaaTCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGc                        >  1:853556/1‑151 (MQ=255)                                                                                                                          aaTCCGGGGGGTGGGGGGGGGAGGGGGG‑ACCACCCAGCGGCTC‑TCCCCCCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACg                         <  1:191767/149‑1 (MQ=255)                                                                                                                          aaTCCCGGGGGGGGGGGGGGGGGTGGGGAATCACCGGGCGCCGT‑TTACCGCCCGCCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACg                         <  1:10338/150‑1 (MQ=255)                                                                                                                                   gggggggggggggAGGGGG‑ACCACCCGGCGGCGC‑TTCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAg              <  2:803613/148‑1 (MQ=255)                                                                                                                                   ggTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGAt                >  1:563569/1‑149 (MQ=255)                                                                                                                                           gggggAGGGGA‑ATCAACCGGCGGCGT‑TTCCCCCCCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCaa      <  2:837996/151‑1 (MQ=255)                                                                                                                                              ggaggggg‑acCACCCAGCGGCGC‑TCCCCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGtt   <  1:903885/151‑1 (MQ=255)                                                                                                                                               gatgggg‑atCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGttt  >  2:549017/1‑151 (MQ=255)                                                                                                                                                 |                                                                                                                                                       GTTGTGGGATGAGTGCACGTAGCGCGGTATCATCCGCCAGCAGATCGTGTTTCAGCCCCTGAATCAACGCCCTGGCGGTGGTGGGCGGTACGGAAGTAATCACATTGAGAAACCACACCGAAATCCAGCGGGTGGGGAGGGGGATGGGG‑ATCAACCAGCGGCGC‑TTACCGCTCACCGCCATAAAATGTTCAAACTGTTGCTGATAACTGAGCACCTCTGGTCCGGCGGCTTCGAAGATGCGGTGTTCGCTGGCCGGATGATCTAACAACGCAACCAGATAGTGCAGCAAGTTT  >  NC_000913/907436‑907728

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑