Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,966,727 C→T P104L (CCG→CTG)  rho → transcription termination factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,966,7270CT98.2% 206.4 / ‑3.9 54P104L (CCG→CTG) rhotranscription termination factor
Reads supporting (aligned to +/- strand):  ref base C (1/0);  new base T (24/29);  total (25/29)
Fisher's exact test for biased strand distribution p-value = 4.63e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.19e-01

GATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTAC  >  NC_000913/3966584‑3966877
                                                                                                                                               |                                                                                                                                                      
gaTATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAaaga                                                                                                                                                  >  2:96645/1‑150 (MQ=255)
  tatTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAgg                                                                                                                                               >  1:817708/1‑151 (MQ=255)
   atTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAgg                                                                                                                                               <  2:371863/150‑1 (MQ=255)
      gcagattggatTTGGTTCCCTCCGTTCCGCAGACAGCTCCTCCCCCCCCGGGCCTGATGCCATTTACGTTTCCCCGAGCGAAATCCGCCGTTTCATCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGa                                                                                                                                                      <  2:188779/134‑1 (MQ=255)
       cAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAAc                                                                                                                                          <  2:915800/151‑1 (MQ=255)
         ggatggttTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGc                                                                                                                                        <  1:53870/151‑1 (MQ=255)
         ggatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGc                                                                                                                                        <  2:551183/151‑1 (MQ=255)
          gatggttTTGGTTTCCTCCGTTCCGCAAACAGCTCCTACCTCGCCGGCCCTGATGCCATCTACGTTCCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCGCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCt                                                                                                                                       <  1:926044/151‑1 (MQ=255)
          gatggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCt                                                                                                                                       >  2:683870/1‑151 (MQ=255)
             ggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTAtt                                                                                                                                    <  2:794617/151‑1 (MQ=255)
             ggatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTAt                                                                                                                                     <  1:186525/150‑1 (MQ=255)
              gatTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGTTGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTAttt                                                                                                                                   <  2:701831/151‑1 (MQ=255)
                   ggTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTgcgc                                                                                                                              >  1:838835/1‑151 (MQ=255)
                        cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCgctgct                                                                                                                          <  1:729364/150‑1 (MQ=255)
                        cctccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCgctgct                                                                                                                          <  2:696537/150‑1 (MQ=255)
                          tccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGa                                                                                                                        >  1:7708/1‑150 (MQ=255)
                           ccGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGaa                                                                                                                       >  2:525543/1‑150 (MQ=255)
                             gTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGt                                                                                                                    >  2:579825/1‑151 (MQ=255)
                               tCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGtt                                                                                                                   >  1:108371/1‑150 (MQ=255)
                                ccGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTa                                                                                                                  <  2:186261/150‑1 (MQ=255)
                                    aGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGaa                                                                                                             <  2:1005016/151‑1 (MQ=255)
                                     gACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAg                                                                                                            <  2:664854/151‑1 (MQ=255)
                                        aGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGtt                                                                                                          >  1:768786/1‑150 (MQ=255)
                                            ccTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACtt                                                                                                     <  2:454741/151‑1 (MQ=255)
                                               aCCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTc                                                                                                    <  2:878569/149‑1 (MQ=255)
                                                     ccGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAGCGAAGTTAACTTCGACAAAcc                                                                                            >  2:191681/1‑151 (MQ=255)
                                                          ccTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaaa                                                                                       <  2:422220/151‑1 (MQ=255)
                                                          ccTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGaaa                                                                                       <  1:272254/151‑1 (MQ=255)
                                                                        cGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGTAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAATCCTGAAAACGCCCGca                                                                              <  1:55019/146‑1 (MQ=255)
                                                                        cGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaacaa                                                                          >  2:841770/1‑150 (MQ=255)
                                                                        cGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaacaa                                                                          >  1:833354/1‑150 (MQ=255)
                                                                        cGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGcaac                                                                            <  2:495293/148‑1 (MQ=255)
                                                                          tttCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAAt                                                                       >  2:10847/1‑151 (MQ=255)
                                                                             ccccTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATcc                                                                     <  2:884983/150‑1 (MQ=255)
                                                                             ccccTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCct                                                                    <  2:339675/151‑1 (MQ=255)
                                                                             ccccTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCct                                                                    <  2:966292/151‑1 (MQ=255)
                                                                                   gCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTg                                                               >  2:613152/1‑150 (MQ=255)
                                                                                          ccgccgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTa                                                       <  1:10846/151‑1 (MQ=255)
                                                                                              cgTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAccc                                                   >  1:792344/1‑151 (MQ=255)
                                                                                               gTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAcccc                                                  <  1:501376/151‑1 (MQ=255)
                                                                                               gTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAAcccc                                                  >  1:648860/1‑151 (MQ=255)
                                                                                                 ttCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGc                                                >  1:174278/1‑151 (MQ=255)
                                                                                                  tCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCt                                               >  2:628680/1‑151 (MQ=255)
                                                                                                   cAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTg                                              >  2:268433/1‑151 (MQ=255)
                                                                                                    aaCCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGc                                             >  2:426526/1‑151 (MQ=255)
                                                                                                         ccGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACg                                          <  2:260079/149‑1 (MQ=255)
                                                                                                         ccGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGc                                         <  1:217647/150‑1 (MQ=255)
                                                                                                              cTGGTGATACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAActc                                   >  2:926110/1‑151 (MQ=255)
                                                                                                                    aTACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTc                              <  1:22670/150‑1 (MQ=255)
                                                                                                                     tACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCt                             >  1:816937/1‑150 (MQ=255)
                                                                                                                     tACCATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTg                            >  1:778511/1‑151 (MQ=255)
                                                                                                                        cATCTCTGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGt                         <  2:844627/151‑1 (MQ=255)
                                                                                                                         aTCTCAGGTAAGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGt                         >  1:940836/1‑150 (MQ=255)
                                                                                                                                  aaGATTCGCCCGCTGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTg               >  1:629961/1‑151 (MQ=255)
                                                                                                                                        cgcccgctGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAAc          <  1:763314/150‑1 (MQ=255)
                                                                                                                                        cgcccgctGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACg         <  2:649176/151‑1 (MQ=255)
                                                                                                                                               cgAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTAc  <  1:628368/151‑1 (MQ=255)
                                                                                                                                               |                                                                                                                                                      
GATATTGCAGGATGGATTTGGTTTCCTCCGTTCCGCAGACAGCTCCTACCTCGCCGGTCCTGATGACATCTACGTTTCCCCTAGCCAAATCCGCCGTTTCAACCTCCGCACTGGTGATACCATCTCTGGTAAGATTCGCCCGCCGAAAGAAGGTGAACGCTATTTTGCGCTGCTGAAAGTTAACGAAGTTAACTTCGACAAACCTGAAAACGCCCGCAACAAAATCCTCTTTGAGAACTTAACCCCGCTGCACGCAAACTCTCGTCTGCGTATGGAACGTGGTAACGGTTCTAC  >  NC_000913/3966584‑3966877

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑