Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,903,497	T→C	I139T (ATC→ACC)	manY →	mannose‑specific enzyme IIC component of PTS

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,903,497	0	T	C	100.0%	174.1 / NA	46	I139T (ATC→ACC)	manY	mannose‑specific enzyme IIC component of PTS
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (20/26);  total (20/26)

CAGAGCATTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTG  >  NC_000913/1903349‑1903627                                                                                                                                                     |                                                                                                                                   caGAGCATTGGAGCAGGGATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGAccc                                                                                                                                  >  1:245316/1‑151 (MQ=255)    agCATTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCCGGACCCACg                                                                                                                               >  2:78/1‑151 (MQ=255)         tGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAAGGGCAACCTGACAGCGATTTCCTGGACCCACGTttc                                                                                                                           <  1:763466/150‑1 (MQ=255)               aGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTg                                                                                                                    >  1:908238/1‑151 (MQ=255)                   aTCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTc                                                                                                                 <  1:880362/150‑1 (MQ=255)                    tCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCt                                                                                                               >  2:714538/1‑151 (MQ=255)                     cGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCt                                                                                                               <  2:361430/150‑1 (MQ=255)                                 cccTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCgt                                                                                                  >  2:1039190/1‑151 (MQ=255)                                         ccGCTGCTGGCCAGGTACTGGCCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCt                                                                                            <  1:624044/149‑1 (MQ=255)                                          cGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTAtt                                                                                         <  2:168452/151‑1 (MQ=255)                                                   ccAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTc                                                                                <  2:158999/151‑1 (MQ=255)                                                     aGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCAt                                                                              >  1:894956/1‑151 (MQ=255)                                                      ggTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATc                                                                             <  2:500095/151‑1 (MQ=255)                                                       gTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCg                                                                            >  1:857724/1‑151 (MQ=255)                                                         aCTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGtt                                                                          >  2:92286/1‑151 (MQ=255)                                                          cTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTg                                                                         >  2:1033126/1‑151 (MQ=255)                                                           tGACCTTCATCGTTCGTACTATTTCCGTTGTTTTCGAGCCTGCTGCGGATAAGGCTGCTGATGAAGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCGCTGTTCCTGCAAGGAATGCGTGTGGCTATTCCGGCCGTCag                                                                              <  1:452746/145‑2 (MQ=255)                                                            gACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGTCTATTCCGGCCGTCATCGTTgc                                                                        <  1:473119/150‑1 (MQ=255)                                                                atcatcGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGTGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGctgt                                                                   <  1:568644/151‑1 (MQ=255)                                                                  catcGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGctgtct                                                                 <  1:739797/151‑1 (MQ=255)                                                                      gttcgtACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTg                                                             <  2:200424/151‑1 (MQ=255)                                                                         cgtACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTa                                                          <  2:445112/151‑1 (MQ=255)                                                                                   ccGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGt                                                <  2:908654/151‑1 (MQ=255)                                                                                     gTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTAc                                              <  1:714192/151‑1 (MQ=255)                                                                                        gCTTTCCAGCGCGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGa                                           <  2:374896/151‑1 (MQ=255)                                                                                          tttCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAAc                                         >  2:742499/1‑151 (MQ=255)                                                                                           ttCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGaa                                          >  1:819627/1‑149 (MQ=255)                                                                                            tCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACAt                                       >  2:822660/1‑151 (MQ=255)                                                                                            tCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACAt                                       >  1:213378/1‑151 (MQ=255)                                                                                             ccAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACAt                                       <  1:1032664/150‑1 (MQ=255)                                                                                             ccAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATg                                      <  1:798349/151‑1 (MQ=255)                                                                                               aGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCATTGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCt                                    <  1:617170/151‑1 (MQ=255)                                                                                                gCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTg                                   <  2:719483/151‑1 (MQ=255)                                                                                                gCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTg                                   >  2:559625/1‑151 (MQ=255)                                                                                                    gCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATg                               <  2:135806/151‑1 (MQ=255)                                                                                                    gCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATg                               >  2:404935/1‑151 (MQ=255)                                                                                                     cTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATg                               >  1:714957/1‑150 (MQ=255)                                                                                                        cGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGAt                           >  1:1037874/1‑151 (MQ=255)                                                                                                           aTAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTcc                        >  2:574428/1‑151 (MQ=255)                                                                                                             aaGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCg                       <  1:952954/150‑1 (MQ=255)                                                                                                               ggCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGaa                    <  2:740220/151‑1 (MQ=255)                                                                                                                  tggtgATAACGGCAACCTGACAGCGATTTCATGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCAGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGCAgtg                 >  1:928643/4‑151 (MQ=255)                                                                                                                   gctgATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAgtg                 <  1:829056/150‑1 (MQ=255)                                                                                                                    ctgATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAgtgg                >  1:479148/1‑150 (MQ=255)                                                                                                                     tgATAACGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAgtggtg              >  2:455149/1‑151 (MQ=255)                                                                                                                           cGGCAACCTGACAGCGATTTCCTGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAAt        <  2:858127/151‑1 (MQ=255)                                                                                                                                 ccTGACAGCGATTTCCCGGACCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTg  <  1:78/151‑1 (MQ=255)                                                                                                                                                     |                                                                                                                                   CAGAGCATTGGTGCAGGTATCGCACTGGCAATCCCTCTGGCCGCTGCGGGCCAGGTACTGACCATCATCGTTCGTACTATTACCGTTGCTTTCCAGCACGCTGCGGATAAGGCTGCTGATAACGGCAACCTGACAGCGATTTCCTGGATCCACGTTTCTTCTCTGTTCCTGCAAGCAATGCGTGTGGCTATTCCGGCCGTCATCGTTGCGCTGTCTGTTGGTACCAGCGAAGTACAGAACATGCTGAATGCGATTCCGGAAGTGGTGACCAATGGTCTG  >  NC_000913/1903349‑1903627

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑