Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,317,438 G→A A30V (GCG→GTG)  nusA ← transcription termination/antitermination L factor

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,317,4380GA98.3% 222.0 / NA 58A30V (GCG→GTG) nusAtranscription termination/antitermination L factor
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (31/26);  minor base C (0/1);  total (31/27)
Fisher's exact test for biased strand distribution p-value = 4.66e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTG  >  NC_000913/3317294‑3317583
                                                                                                                                                |                                                                                                                                                 
tCAAGGGCGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCttt                                                                                                                                             >  1:451083/1‑151 (MQ=255)
 cAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCttt                                                                                                                                             <  1:35669/150‑1 (MQ=255)
 cAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTc                                                                                                                                            >  2:392815/1‑151 (MQ=255)
        gATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCt                                                                                                                                     <  2:698207/151‑1 (MQ=255)
             ccTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGaa                                                                                                                                <  1:925038/151‑1 (MQ=255)
             ccTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGa                                                                                                                                 <  2:931448/150‑1 (MQ=255)
                 ggTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAAt                                                                                                                             <  2:907853/150‑1 (MQ=255)
                     ggCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAAtcttct                                                                                                                        >  1:791277/1‑151 (MQ=255)
                        tGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTcgc                                                                                                                     >  2:731190/1‑151 (MQ=255)
                          ggTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTcgcg                                                                                                                    <  1:535866/150‑1 (MQ=255)
                          ggTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGa                                                                                                                   <  2:882752/151‑1 (MQ=255)
                             gACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGt                                                                                                                <  2:933925/151‑1 (MQ=255)
                                       acaacTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCtttt                                                                                                      >  1:15759/1‑151 (MQ=255)
                                        caacTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTc                                                                                                     >  2:546718/1‑151 (MQ=255)
                                           cTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCAt                                                                                                   <  1:473222/150‑1 (MQ=255)
                                            tAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTg                                                                                                 >  1:745316/1‑151 (MQ=255)
                                            tAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGAAGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTg                                                                                                 >  1:900587/1‑151 (MQ=255)
                                                cAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATa                                                                                             <  1:30544/151‑1 (MQ=255)
                                                     aCGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCt                                                                                        >  1:1009561/1‑151 (MQ=255)
                                                        gAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTTTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCa                                                                                     <  1:32429/151‑1 (MQ=255)
                                                                 aaaaTCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGcc                                                                            >  2:870853/1‑151 (MQ=255)
                                                                     tCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCaaa                                                                         >  2:17286/1‑150 (MQ=255)
                                                                          gCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCt                                                                   >  1:895467/1‑151 (MQ=255)
                                                                           cTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCtt                                                                  >  1:68441/1‑151 (MQ=255)
                                                                           cTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCtt                                                                  >  2:426413/1‑151 (MQ=255)
                                                                             tttGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCCCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTGTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCttt                                                                 <  1:176836/150‑1 (MQ=255)
                                                                             tttGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCtt                                                                  <  2:222696/149‑1 (MQ=255)
                                                                              ttGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGt                                                               >  2:305215/1‑151 (MQ=255)
                                                                                  gatcgatcTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCAt                                                           >  1:36406/1‑151 (MQ=255)
                                                                                  gatcgatcTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCAt                                                           >  2:210379/1‑151 (MQ=255)
                                                                                           gTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATcgc                                                        <  2:584362/145‑1 (MQ=255)
                                                                                           gTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCgg                                                      <  2:853480/147‑1 (MQ=255)
                                                                                                 ggACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCAcc                                            >  2:703264/1‑151 (MQ=255)
                                                                                                  gACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCt                                           >  1:17301/1‑151 (MQ=255)
                                                                                                  gACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCt                                           <  2:793651/151‑1 (MQ=255)
                                                                                                   aCGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTc                                          <  2:963381/151‑1 (MQ=255)
                                                                                                      tCGATCTCTTGTTCATCTTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCACAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATc                                       >  1:231601/1‑151 (MQ=255)
                                                                                                      tCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATc                                       >  2:432202/1‑151 (MQ=255)
                                                                                                       cGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATcc                                      >  2:325575/1‑151 (MQ=255)
                                                                                                             ctTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCa                                     <  1:589280/146‑1 (MQ=255)
                                                                                                               tGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTa                                <  1:751192/149‑1 (MQ=255)
                                                                                                               tGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTAt                               >  1:160200/1‑150 (MQ=255)
                                                                                                                gTTCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTa                             <  2:1034296/151‑1 (MQ=255)
                                                                                                                 ttCATATTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTACATCGCGGGCTTTTCACCTCATCCAGACTATTa                             >  2:607435/1‑150 (MQ=255)
                                                                                                                    atatTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAg                         <  2:68462/151‑1 (MQ=255)
                                                                                                                     tatTTTTTCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGt                        >  1:27864/1‑151 (MQ=255)
                                                                                                                          tttCTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTgggg                    <  2:15762/150‑1 (MQ=255)
                                                                                                                             cTTTGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAAcc                >  2:190587/1‑151 (MQ=255)
                                                                                                                              tttGTTGCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAAcc                >  2:328563/1‑150 (MQ=255)
                                                                                                                               ttgttgCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCa               <  1:21530/150‑1 (MQ=255)
                                                                                                                                tgttgCTGTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACGACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAg              <  2:576387/150‑1 (MQ=255)
                                                                                                                                       gTCGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGcc       <  2:783269/150‑1 (MQ=255)
                                                                                                                                         cGCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCtt     <  2:1007717/150‑1 (MQ=255)
                                                                                                                                          gCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCt   <  1:514530/151‑1 (MQ=255)
                                                                                                                                          gCCAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATATCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCt   >  1:283571/1‑151 (MQ=255)
                                                                                                                                           ccAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTg  >  1:887568/1‑151 (MQ=255)
                                                                                                                                           ccAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTg  >  1:306096/1‑151 (MQ=255)
                                                                                                                                           ccAGCACGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTg  >  2:883348/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TCAAGGGTGATTTCCTTGGTCGGCTGGGTGACTTCATCAACAACTAACCAGCGACGGAAAGTGTCAAAATCACCGCTTTTGCGATCGATCTGTACGCGGACGTCGATCTCTTGTTCATATTTTTTCTTTGTTGCTGTCGCCAGCGCGCTTTCCAATGCTTCGAAAATCTTCTCGCGAGGTAGCGCCTTTTCATTGGATACGGCTTCAACTACAGCCAAAATTTCTTTGTTCATCGCGGGCTTTTCACCTCATCCAGACTATTAAAAGTGGGGAACCAGGTTCGCCTTCTG  >  NC_000913/3317294‑3317583

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑