Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,530,902	A→G	V513A (GTC→GCC)	yhgE ←	DUF4153 family putative inner membrane protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,530,902	0	A	G	100.0%	228.8 / NA	56	V513A (GTC→GCC)	yhgE	DUF4153 family putative inner membrane protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (31/25);  total (31/25)

AAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGACTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAGAATGTCGC  >  NC_000913/3530757‑3531044                                                                                                                                                  |                                                                                                                                               caGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTg                                                                                                                                           >  1:770061/2‑151 (MQ=255)  aGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGt                                                                                                                                          <  2:590885/151‑1 (MQ=255)    aTCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGtca                                                                                                                                        <  1:139846/151‑1 (MQ=255)       gcgcCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGtcatca                                                                                                                                     <  2:88997/151‑1 (MQ=255)       gcgcCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGtcatca                                                                                                                                     <  2:169098/151‑1 (MQ=255)              gCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTaaa                                                                                                                                >  1:71094/1‑149 (MQ=255)               cTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGc                                                                                                                             >  2:567416/1‑151 (MQ=255)                 ttAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCa                                                                                                                            >  1:334149/1‑150 (MQ=255)                      cTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAgg                                                                                                                      >  1:563355/1‑151 (MQ=255)                        tCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAgg                                                                                                                      <  1:680149/149‑1 (MQ=255)                         cGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGAtc                                                                                                                   >  1:118434/1‑151 (MQ=255)                          gTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGAtcc                                                                                                                  >  1:285701/1‑151 (MQ=255)                          gTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGAtcc                                                                                                                  >  1:716775/1‑151 (MQ=255)                             cccAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGAtccgct                                                                                                               >  1:987247/1‑151 (MQ=255)                                        aTCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCa                                                                                                    <  1:838523/151‑1 (MQ=255)                                           cTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATc                                                                                                  >  1:36739/1‑150 (MQ=255)                                             tCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACt                                                                                               >  1:831736/1‑151 (MQ=255)                                                cAGCTGTGAGTAATTTTTCTTTCGACATTTCGCTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCACAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTAt                                                                                             >  1:47616/1‑150 (MQ=255)                                                   cTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTc                                                                                           >  2:614965/1‑149 (MQ=255)                                                       gAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCt                                                                                     >  2:917824/1‑151 (MQ=255)                                                            aTTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTc                                                                                >  2:401280/1‑151 (MQ=255)                                                                      cGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGAc                                                                       <  2:786827/150‑1 (MQ=255)                                                                       gTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGc                                                                     >  1:988660/1‑151 (MQ=255)                                                                            ttcgttcgGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGc                                                                 <  1:450594/150‑1 (MQ=255)                                                                            ttcgttcgGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGc                                                                 <  2:942249/150‑1 (MQ=255)                                                                            ttcgttcgGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCg                                                                >  2:155484/1‑151 (MQ=255)                                                                             tcgttcgGAAGTAAACCCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTATAAGCAAACAGGATCCGCTCCGCCTGATCATCACTATTCAGATCTTGCTCGACCAAGACGCAGGCg                                                                <  1:313740/150‑1 (MQ=255)                                                                                  cgGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCttt                                                           <  2:940204/150‑1 (MQ=255)                                                                                   ggAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCttttt                                                         >  2:726747/1‑151 (MQ=255)                                                                                   ggAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCttttt                                                         <  1:597687/151‑1 (MQ=255)                                                                                      aGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCaa                                                      <  2:266568/151‑1 (MQ=255)                                                                                         aaaCTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCCATa                                                    <  1:94021/150‑1 (MQ=255)                                                                                             tCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACACTGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGt                                               >  1:161708/1‑151 (MQ=255)                                                                                              cATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGt                                               <  2:71115/150‑1 (MQ=255)                                                                                               atatCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGtca                                             <  2:893715/151‑1 (MQ=255)                                                                                                tatCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGtcat                                            >  2:480510/1‑151 (MQ=255)                                                                                                   cAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGtcatca                                          <  2:606721/150‑1 (MQ=255)                                                                                                       cgcgTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTta                                     <  2:504847/151‑1 (MQ=255)                                                                                                        gcgTCCCATTCTGTTCTGTCTGAGTCAAAGCTATAGACAATGGCTCTGTCATCATTAATAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTtat                                    >  1:415436/1‑151 (MQ=255)                                                                                                            cccATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCg                                >  2:63479/1‑151 (MQ=255)                                                                                                            cccATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCg                                <  1:850743/151‑1 (MQ=255)                                                                                                              cATTCTTTTCTGTCTGAGTCAAAGACATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCAGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGa                               >  1:804786/1‑150 (MQ=255)                                                                                                                 tCTTTTCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCt                           >  1:781720/1‑151 (MQ=255)                                                                                                                    tttCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATTACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCt                           <  1:114790/148‑1 (MQ=255)                                                                                                                    tttCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTgg                         >  1:335114/1‑150 (MQ=255)                                                                                                                     ttCTGTCTGAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGAt                       >  2:73014/1‑151 (MQ=255)                                                                                                                      tctgtctgAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGAtt                      >  1:582929/1‑151 (MQ=255)                                                                                                                         gtctgAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTa                     >  2:170214/1‑149 (MQ=255)                                                                                                                         gtctgAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTa                     <  2:1009103/149‑1 (MQ=255)                                                                                                                             gAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGcc                <  2:991586/150‑1 (MQ=255)                                                                                                                             gAGTCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCg               >  1:420007/1‑151 (MQ=255)                                                                                                                                tCAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTCAGGCCGAc             >  1:775476/1‑150 (MQ=255)                                                                                                                                 cAAAGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCa           <  2:726176/151‑1 (MQ=255)                                                                                                                                   aaGCCATAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCATGTTATAGCGATCCTGGATTAAGGCCGACCAGa         <  2:469842/151‑1 (MQ=255)                                                                                                                                        aTAGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAGAATGTc    <  2:537948/151‑1 (MQ=255)                                                                                                                                          aGACAATGGCTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAGAATGTCGc  >  2:425757/1‑151 (MQ=255)                                                                                                                                                  |                                                                                                                                               AAGATCGCGCCACGCTTTAGGCTTCGTCCCCAGTTTTCCATCCTTCGCAGCTGTGAGTAATTTTTCTTTCGTTATTTCGTTCGGAAGTAAACTCATATCAAGCGCGTCCCATTCTTTTCTGTCTGAGTCAAAGCCATAGACAATGACTCTGTCATCATTAAAAGCAAACAGGATCCGCTCCGCCTGACCATCACTATTCAGATCTTGCTCGACCAGGACGCAGGCGTCTTTTTCAATACAGGTCATCACGTTATAGCGATCCTGGATTAAGGCCGACCAGAATGTCGC  >  NC_000913/3530757‑3531044

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑