Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913898,5980TA48.0% 17.4 / 10.4 25A27A (GCT→GCArlmC23S rRNA m(5)U747 methyltransferase, SAM‑dependent
Reads supporting (aligned to +/- strand):  ref base T (10/3);  new base A (0/12);  total (10/15)
Fisher's exact test for biased strand distribution p-value = 1.08e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CGGCGGTTCTTCCAGTTGCAATAATGTGTATAATCGTCGCCCCTGATGATGTGAAGGTCAATGTATGCAGTGCGCACTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAATGCAGCCGATTCCAGAGCAACTCTCCGCTAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGTTTCGTAATAAAGCCAAAATGGTGGTGAGTGGTAGCGTTGAAAAACCACTGCTCGGTAT  >  NC_000913/898454‑898738
                                                                                                                                                |                                                                                                                                            
cggcggTTCTTCCAGTTGCAATAATGTGTATAATCGTCGCCCCTGATGATGTGAAGGTCAATGTATGCAGTGCGCACTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAATGCAGCCGATTCCAGAGCAACTCTCCGCTAAAAcc                                                                                                                                        >  1:3725/1‑151 (MQ=255)
    ggTTCTTCCAGTTGCAATAATGTGTATAATCGTCGCCCCTGATGATGTGAAGGTCAATGTATGCAGTGCGCACTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGTTAATGCAGCCGATTCCAGAGCAACTCTCCGCTAAAAccgccg                                                                                                                                    >  1:353398/1‑151 (MQ=255)
                           gtATAATCGTCGCCCCTGATGATGTGAAGGTCAATGTATGCAGTGCGCACTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAATGCAGCCGATTCCAGAGCAACTCTCCGCTAAAACCGCCGATCTTAAAAATCTGCTCGCCGAc                                                                                                             >  2:120427/1‑151 (MQ=255)
                             ataATCGCCCCCCGTGATGATGTGAAGGTAAATGTATGCGGGGCGCATTTGACGGGGCGGGTCTCTTTCTTCCCTGTCAGGGGATAATACAGCCGTTCCCAGAGAACCTCCCCGCAAAAACCGCCGATCTTAAAAATCTGCTCGCCGACtt                                                                                                           <  1:270963/151‑1 (MQ=255)
                               atccgccgCCCCGGATGATGTGAGGGTAAATGTATGCAGTGCGCATTTTCCGACGCGGGTCGTTGTCGTCCCGGTAAGTGGATAATGAAGCCGATTCCAGAGCAACTCCCCGCAAAAACCGCCGATCTTAAAAATCTGCTc                                                                                                                   <  1:316489/135‑1 (MQ=255)
                                       ccccTGATGATGTGAAGTTAAATGTATGCAGTGCGCCCTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAATGCAGCCGATTCCAGAGCACCTCTCCGCTAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGa                                                                                                  <  2:118656/150‑1 (MQ=255)
                                            gatgatGTGAAGGTCAATGTATGCAGTGCGCACTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAATGCAGCCGATTCCAGAGCAACTCTCCGCTAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAAt                                                                                             >  2:183258/1‑150 (MQ=255)
                                               gatgTGAAGGTCAATGTATGCAGTGCGCACTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAATGCAGCCGATTCCAGAGCAACTCTCCGCTAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTg                                                                                         >  2:509897/1‑151 (MQ=255)
                                                 tgtgAAGGTAAATGTATGCAGTGCGCATTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAATGCAGCCGATTCCAGAGCAACTCCCCGCAAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGt                                                                                          <  2:314996/148‑1 (MQ=255)
                                                            aTGTATGCAGTGCGCACTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAATGCAGCCGATTCCAGAGCAACTCTCCGCTAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTc                                                                             >  1:536948/1‑150 (MQ=255)
                                                            aTGTATGCAGTGCGCACTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAATGCAGCCGATTCCAGAGCAACTCTCCGCTAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTc                                                                             >  2:469766/1‑150 (MQ=255)
                                                                        cgcATTTTACGCCGCGGGTCGCTGTCGTTCCTGTCAGTGGATAATGCAGCCGATCCCAGAGCAACTCCCCGCAAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACa                                                                 <  2:235040/150‑1 (MQ=255)
                                                                          cgcTTTACGACGCGGGTCGCTGTCTTTCCTGTCAGGGGATAATGCAGCCGATTCCAGACCACCTCTCCGAAAAAGCCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACa                                                                 <  1:62980/146‑1 (MQ=255)
                                                                              tgacgaggGGGTTCGCTGTCGTTCCTGAAAGTGAATAATGCACCCTATCCGAGAGCACCTCTCCGCAAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGtt                                                           <  1:481326/148‑1 (MQ=255)
                                                                              taccgccgcgGTTCGTTGTCGTCCCTGTCAGGGAATAGTGCGGCCGTTCCCAGAGCACCTCCCCCAAAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGtt                                                           <  1:499574/144‑1 (MQ=255)
                                                                                     gcgcgccGCTGCCGTCCCTGTCAGTGGATAATGCAGCCTATCCCAGACAAACTCCCCGAAAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGTTTCGtaata                                                   <  1:335046/145‑1 (MQ=255)
                                                                                       gggTCGCTGTCGTTCCTGTCAGTGAATAATGCAGCCGTTTCCAGAGCACCTCTCCCCTAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGTTTCGtaat                                                    <  2:124087/148‑1 (MQ=255)
                                                                                       gggTCGCTGTCGTTCCTGTCAGTGAAAAATGCAGCCGATTCAAGAGCACCTCCCCGCAAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGttt                                                          <  2:187175/142‑1 (MQ=255)
                                                                                                  gTTCCTGTCAGTGGATAATGCAGCCGATTCCAGAGCAACTCTCCGCTAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGTTTCGTAATAAAGCCAAAAtgg                                       >  2:165730/1‑150 (MQ=255)
                                                                                                     ccTGTCAGTGGATAATGCAGCCGATTCCAGAGCAACTCTCCGCTAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGTTTCGTAATAAAGCCAAAATggtggt                                   >  2:97083/1‑151 (MQ=255)
                                                                                                     ccTGTCAGTGGATAATGCAGCCGATTCCAGAGCAACTCCCCGCAAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGTTTCGTAATAAAGCCAAAAtg                                        <  1:99910/146‑1 (MQ=255)
                                                                                                         taaggggAAAATGCGGCCGATCCCAGACCAACCCTCCGCTAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGTTTCGTAATAAAGCCAAAATGGTGGTGa                                 <  1:95127/144‑1 (MQ=255)
                                                                                                                  aaggggccctatcccAGAGCAACTCCCCGCAAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGTTTCGTAATAAAGCCAAAATGGTGGTGAGTGGt                            <  1:509164/132‑1 (MQ=255)
                                                                                                                                  gaacacccctcCGCAAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGTTTCGTAATAAAGCCAAAATGGTGGTGAGTGGTAGCGTTGAAAAACCACTGCt        <  2:353492/141‑1 (MQ=255)
                                                                                                                                      aaCTCTCCGCTAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGTTTCGTAATAAAGCCAAAATGGTGGTGAGTGGTAGCGTTGAAAAACCACTGCTCGGTAt  >  1:40156/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                            
CGGCGGTTCTTCCAGTTGCAATAATGTGTATAATCGTCGCCCCTGATGATGTGAAGGTCAATGTATGCAGTGCGCACTTTACGACGCGGGTCGCTGTCGTTCCTGTCAGTGGATAATGCAGCCGATTCCAGAGCAACTCTCCGCTAAAACCGCCGATCTTAAAAATCTGCTCGCCGACTTTCCGGTTGAGGAATGGTGCGCGCCGGTGTCAGGCCCGGAACAAGGGTTTCGTAATAAAGCCAAAATGGTGGTGAGTGGTAGCGTTGAAAAACCACTGCTCGGTAT  >  NC_000913/898454‑898738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: