Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,206,4350CT50.0% 14.6 / 29.5 36intergenic (‑179/‑28)ttdR/ttdAtranscriptional activator of ttdABT/L‑tartrate dehydratase, alpha subunit
Reads supporting (aligned to +/- strand):  ref base C (1/17);  new base T (18/0);  total (19/17)
Fisher's exact test for biased strand distribution p-value = 4.19e-09
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206305‑3206585
                                                                                                                                  |                                                                                                                                                       
tGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTg                                                                                                                                      <  1:57714/150‑1 (MQ=255)
 gCCATGCCCTTCACTGGCGCAATATTCATGCTTATTTACGCGTTGACTCTCGAACTGTTGATTACATAGTCACGGTGGCAAAAAATATAAAACGACAAATTTAGTGGTAGTTTGTGGTATGGCGTTTTTCCAGTCAAATACAAAAACTga                                                                                                                                     <  1:124561/150‑2 (MQ=255)
    aTTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATTTTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGtt                                                                                                                                 <  1:91002/151‑1 (MQ=255)
     ttCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCTGACAAAAAAAAAAAATgggggt                                                                                                                                 >  1:335510/1‑146 (MQ=255)
         cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCATACAaaaaaaaaaa                                                                                                                                         >  1:474947/1‑138 (MQ=255)
         cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGAaaaaaaaaaaaaattgtgttttcat                                                                                                                            >  1:339674/1‑138 (MQ=255)
         cTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGGGGATTTTTTCACAAAAAAAAAAAAAAAGGAGTTgtc                                                                                                                              >  1:445214/1‑147 (MQ=255)
          tGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGATgtcctg                                                                                                                           >  2:378705/1‑146 (MQ=255)
              ctGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCatgat                                                                                                                         <  2:467521/149‑1 (MQ=255)
               tGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATAAAAAAACTGGAGTTTCCat                                                                                                                            >  2:131772/1‑145 (MQ=255)
                ggCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAg                                                                                                                      <  1:54310/150‑1 (MQ=255)
                 gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCACACAAAAAAAAAAACTGGaagaacggga                                                                                                                           >  2:476890/1‑135 (MQ=255)
                 gcgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGa                                                                                                                                    <  1:476770/135‑1 (MQ=255)
                  cgcAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGa                                                                                                                   <  2:496387/151‑1 (MQ=255)
                    cAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGaaa                                                                                                                 <  1:316706/151‑1 (MQ=255)
                     aaTATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAAATTTTTCCGACAAAAAAAAAAAAAGGAGGTGGCATGATGAggg                                                                                                                    >  2:113165/1‑145 (MQ=255)
                                    tttCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAgg                                                                                                  <  1:489128/150‑1 (MQ=255)
                                             gATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCTCACAAAAACAAAAAACGGGGTTGGCAAGATGAGGGAAAAAAATAAAAAACaag                                                                                                  >  2:255465/1‑139 (MQ=255)
                                                    gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACa                                                                                 <  1:265316/151‑1 (MQ=255)
                                                    gAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGGGAATAAGtt                                                                                     >  2:513023/1‑147 (MQ=255)
                                                      aCTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGTTGCCATGATGAGCGAAAGTAATAAACAAAAAGCAGGGAATAAGTTGAc                                                                                  >  2:188688/1‑148 (MQ=255)
                                                        tGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAAAAAAAAATGGAGGTGCCATGATGAGCGGAAATAATAAACAACAGGCAGGGAAAAAGTTTACa                                                                                 >  2:144126/1‑147 (MQ=255)
                                                              ttACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGGAATTTTTTAAACAAAAAAAAAAAAATGAGTTGCCCTGGTTGGGGGAAGAAAAAAAGAAAAAGAAGTGGATAAATTGAAAGAGGTTGttg                                                                       >  1:30867/1‑149 (MQ=255)
                                                                    atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AAc                                                                 <  1:102043/150‑1 (MQ=255)
                                                                    atTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACt                                                                <  1:144088/151‑1 (MQ=255)
                                                                         cGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAACAAAAACTGGAGGTGCCCTGGTGAGGGGAAGGAAAAAAAAAAAAGCAGTGAATAAATTGACAGAGATTTTCGCT‑AACttt                                                              >  2:41514/1‑148 (MQ=255)
                                                                          ggTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCg                                                          <  2:89428/151‑1 (MQ=255)
                                                                               cAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAATACAAAAAATGGAGGTGCCATGATGAGGGAAAGGAAAAAACAACAAGCAGGGAAAAAATTGACAGAGATTTTCGCT‑AACTTTAACGCCCTg                                                     >  2:187905/1‑151 (MQ=255)
                                                                                    aaTATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTTCAAACAAAAAAAAAAAATTGGGGTGCCCTGATGAGCGAAAAGAATAAACAACAAGCAGGGAAAAAATTTGAAGAGATTGTTGCC‑TACTTTACCCCCCTGAtttt                                                >  2:122464/1‑150 (MQ=255)
                                                                                           aaCCACATTTTTAGTGGTAGTTTGTGGCGGTGGATTTTTTCAGACAAAAACAAAAAATTGAGGTGGCATGTTGAGCGGAAGAAATAAAGAAAAGGGAGGGAAAAAATTTGCAGAGGATGTTGCTAAACTTTA‑CGCCATGATTTTTAccc                                           >  2:330344/1‑149 (MQ=255)
                                                                                                caTTTTTAGTGGTAGTTTGTGGGGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAATTAATAACCAACAGGCAGTGAATAAGTTGAAAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATg                                      <  2:166639/149‑1 (MQ=255)
                                                                                                  tttttAGTGGTAGTTTGTGGCGGTGAAATTTTTCAGACAAAAAAAAAAAATGGGGTTGGCATGATGAGGGAAAGTAAAAAGCAAAAAGGAGTGAATAAAGTGACAAAGAATGTTGCC‑AAATTTTTCGCCAAGATTTTTACCCGAAAGcc                                    >  2:432520/1‑149 (MQ=255)
                                                                                                    tttAGTGGTAGTTTGTGGCGGTGAATTTTTTCAGACAAAAACAAAAAATGGAGGTGCCATGATGAGGGGAAGTAATAAGCAAAAAGCAGTGAATAAATTGACAGAGAATGTCGCT‑AACTTTTCCGCCCTGATTTTTTCCCGAATGCCtgat                                >  2:88057/1‑151 (MQ=255)
                                                                                                          ggTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCtgatga                              <  1:359613/147‑1 (MQ=255)
                                                                                                             attttGTGGCGGTGAATTTTTCTAAAAAAATACAAAATCGGGAGTTGCAAGGTGGAGCGAAATTAATAAGCAACAGGCATTAAATAAGTTGCCAAATTTTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACgtggt                        <  2:76035/148‑1 (MQ=255)
                                                                                                                        gTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTaaa            <  2:232274/151‑1 (MQ=255)
                                                                                                                                  ccAGAAAAAAAAAAAAACGGGAGTTGCCATGATGAGAGAAATAAATAAGCAACAGGCAGTAAATAAGTTGACAGAGATTGTCGCT‑ATCTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAg  <  1:113133/151‑1 (MQ=255)
                                                                                                                                  |                                                                                                                                                       
TGCCATTCCCTGCTCTGGCGCAATATTCATTCTTATTTTCGCGTTGATTCACGAACTGTTAATTACATATTCACGGTGGCAAAAAATATAAAACCACATTTTTAGTGGTAGTTTGTGGCGGTGAATTTTTCCAGACAAATACAAAAACTGGAGTTGCCATGATGAGCGAAAGTAATAAGCAACAGGCAGTGAATAAGTTGACAGAGATTGTCGCT‑AACTTTACCGCCATGATTTCTACCCGAATGCCTGATGACGTGGTGGATAAACTAAAACAGCTAAAG  >  NC_000913/3206305‑3206585

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑