Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,538,101	0	G	T	45.5%	53.0 / 15.9	33	T222N (ACC→AAC)	nanM	N‑acetylneuraminic acid mutarotase
Reads supporting (aligned to +/- strand):  ref base G (16/2);  new base T (0/15);  total (16/17)
Fisher's exact test for biased strand distribution p-value = 1.47e-07
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGCTTATCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTG  >  NC_000913/4537955‑4538246                                                                                                                                                    |                                                                                                                                                    tGCTTATCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCTAGGttt                                                                                                                                                  <  1:225445/150‑1 (MQ=255)        ccccGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑t                                                                                                                                         <  1:314474/151‑1 (MQ=255)              aaaaCCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTAtt                                                                                                                                    >  2:6260/1‑150 (MQ=255)                          tACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAAccgccg                                                                                                                        >  2:67261/1‑149 (MQ=255)                                 tCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCg                                                                                                               >  1:253358/1‑151 (MQ=255)                                 tCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCg                                                                                                               >  1:268278/1‑151 (MQ=255)                                          gagaCGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTAcc                                                                                                      >  1:23046/1‑151 (MQ=255)                                             aCGGGAGCAAGTTTATTCCATTTAAAATTATTCCCGGTAAAATCAATTTCAAATCCGCCATCCGTCCGCAACCCTGTTTTGGCTCCCCCATT‑AATAAGCCAGTTTTTACCCCT‑TTTATC‑CACAACCGCCGCACCAGCCGTTCCGTACCAg                                                                                                    <  2:135437/150‑1 (MQ=255)                                               gggAGCAAGTTTTTCCCTTTTAAAATTATTCCGGGTAAAACAAATTTAAAATACGCCACCCTTTCGAAACCCGGGTTGGGCTTCCCCATA‑AAAAACCCAGTTTTTATCCCT‑TTTATC‑CACAACCGCCGCACCAGCCGTTCCGTAcc                                                                                                      <  2:437955/146‑1 (MQ=255)                                                         tttttCCTTTTTAAATTATTCCGGGTAAAATCAATTTAAAATAGGCCTTCCGTCAGCAATCTTGTTTTGGTTTCCCCATT‑AATAAGCGAGTTTTTATCCCT‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCa                                                                                        <  2:537800/147‑1 (MQ=255)                                                           aTTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGCACCAGGGCGATTCGCCAGCg                                                                                     >  2:303391/1‑151 (MQ=255)                                                               cATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAAc                                                                                 >  2:472123/1‑151 (MQ=255)                                                                     aatttatacccggaaaaaTCAATTTAAAACACGTCCTCCGTTCGCATCCGTTTTTTGGCTTCCCCATT‑AATACGCCTGTTTTTATCCCC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCAtt                                                                           <  1:278595/136‑1 (MQ=255)                                                                           ttccgggTAAAATAAAGTTAAAATACGGCACCCGTCCCCAACCCTGTTTTGGCTCCGCCATA‑AATAACCCGGTTTTTACCCCT‑TTTATC‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgt                                                                      <  2:454767/145‑1 (MQ=255)                                                                             aCCGGTAAAATCAAGTTAAAATCCGCCTCCCGTCCGCAATCTTGTTTGGGCTCCCCCATT‑AATAACCCAGTTTTTATCCCT‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCtgtg                                                                     <  2:224373/149‑1 (MQ=255)                                                                               cGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTg                                                                  >  2:255743/1‑150 (MQ=255)                                                                                     aaTCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATc                                                           >  2:125946/1‑151 (MQ=255)                                                                                         aaGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaaa                                                        >  1:376635/1‑150 (MQ=255)                                                                                         aaGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCaaa                                                        >  2:175489/1‑150 (MQ=255)                                                                                                 atcgggCACCCTTCCGCATTCCT‑GTTTTGCTTCCCCCTTAAAAAAGCCGGTTTTTATCCCC‑TTTATC‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAg                                               <  2:58450/147‑1 (MQ=255)                                                                                                    cGGCATCCGTTCGCAATCCTGTTTTGGCTCCGCAATT‑AAAAAGCCGGTTTTTATCCCC‑TTTATT‑CAAAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGaa                                             <  2:121091/150‑1 (MQ=255)                                                                                                    cGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGaa                                             >  1:197437/1‑150 (MQ=255)                                                                                                     ggCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAAc                                           >  2:91269/1‑151 (MQ=255)                                                                                                               cccAATCGGGGTTTGGCTTCCCCATA‑AAAAGCCCAGTTTTTACCCCT‑TTTATC‑CCCACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTgaaga                                 <  2:44341/149‑1 (MQ=255)                                                                                                                  aaTCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACGAGGGCGATTCGCCCCCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAAAAACTTATTGAAGAaat                              >  1:21231/1‑151 (MQ=255)                                                                                                                             gcctcccccATT‑AATAACCCAGTTTTTACCCCT‑TTTATC‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTg                     <  1:107806/142‑1 (MQ=255)                                                                                                                                 tCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCttttt               >  2:131269/1‑151 (MQ=255)                                                                                                                                 cccccATA‑AAAAAGCCGGTTTTTATCCCT‑TTTATC‑AAAACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCttt                 <  1:406554/146‑1 (MQ=255)                                                                                                                                  ccccATT‑AATACGCCAGTTTTTATCACT‑TTTTTT‑CACAGCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttttt              <  2:268354/149‑1 (MQ=255)                                                                                                                                  ccccATA‑AATAAGCCGGTTTTTATCCCT‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtt                  <  2:487162/145‑1 (MQ=255)                                                                                                                                   gCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTg             >  2:216449/1‑151 (MQ=255)                                                                                                                                          aaaaggCCAGTTTTTCCCCCC‑TTTATC‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCtttt                <  1:182942/137‑1 (MQ=255)                                                                                                                                               acccggTTTTAACCCC‑CTTATTCCACACCCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATa     <  1:295889/145‑1 (MQ=255)                                                                                                                                                ccAGTTTTTATCCCC‑TTTATC‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAAt      <  2:398636/146‑1 (MQ=255)                                                                                                                                                 ccagTTTTTTCCCCTTTTATC‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTg  <  1:247174/147‑1 (MQ=255)                                                                                                                                                    |                                                                                                                                                    TGCTTATCCCCGCAAAACCGCCAGCTACGCCATCTGGTGATGAGACGGGAGCAAGCTTATTCCATTTTAAATTATTACCGGTGAAATCAAGTTCAAATACGGCATCCGTTCGCAATCCTGGTTTGGCTTCGCCATT‑AATAAGCCAGGTTTTATCACC‑TTTATT‑CACAACCGCCGCACCAGCCGTTCCGTACCAGGGCGATTCGCCAGCGTAACTCCATTGCTGTGTTGAGGGATCAAAAGACAACAGAAACTTATTGAAGAAATAATCTTCTGCTTTTTTGTCAAAATAGTG  >  NC_000913/4537955‑4538246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑