Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,810,804	Δ1 bp	coding (35/531 nt)	mprA →	transcriptional repressor of microcin B17 synthesis and multidrug efflux

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,810,804	0	T	.	100.0%	249.2 / NA	51	coding (35/531 nt)	mprA	transcriptional repressor of microcin B17 synthesis and multidrug efflux
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base . (26/25);  total (26/25)

GAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCG  >  NC_000913/2810658‑2810949                                                                                                                                                   |                                                                                                                                                  gAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCaaaat                                                                                                                                               >  2:148389/1‑150 (MQ=255) gAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCaaaat                                                                                                                                               <  2:234971/151‑2 (MQ=255)     gTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTcg                                                                                                                                          <  2:21691/151‑1 (MQ=255)        aTGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCgcgc                                                                                                                                       <  1:367081/151‑1 (MQ=255)           gCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGcc                                                                                                                                      >  1:749300/1‑149 (MQ=255)              aTTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAgccg                                                                                                                                 <  2:379908/151‑1 (MQ=255)                 atataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAgccgcc                                                                                                                               <  2:770250/150‑1 (MQ=255)                 atataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAgccgcc                                                                                                                               >  1:704247/1‑150 (MQ=255)                   ataACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACg                                                                                                                            >  1:598338/1‑151 (MQ=255)                     aaCCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGaa                                                                                                                          <  2:463161/151‑1 (MQ=255)                           caAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGAtttt                                                                                                                    >  1:361071/1‑151 (MQ=255)                             aGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTcc                                                                                                                  <  1:845771/151‑1 (MQ=255)                              gAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCt                                                                                                                 >  2:32316/1‑151 (MQ=255)                               aaTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt                                                                                                                <  1:373779/151‑1 (MQ=255)                               aaTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt                                                                                                                <  1:539197/151‑1 (MQ=255)                                aTCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCtt                                                                                                                >  1:690240/1‑150 (MQ=255)                                 tCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTAt                                                                                                              >  1:778786/1‑151 (MQ=255)                                  cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTAt                                                                                                              <  1:87027/150‑1 (MQ=255)                                  cATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTAt                                                                                                              <  1:830615/150‑1 (MQ=255)                                    tttttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATc                                                                                                             >  2:270530/1‑149 (MQ=255)                                      tttCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg                                                                                                          <  2:820454/150‑1 (MQ=255)                                        tCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAgg                                                                                                          >  1:166868/1‑148 (MQ=255)                                           aaaaCAAGACATTTGCTTTATTTGTCACTGTCGTTACTATCTCGGCTGAAATTAATGAGGTCATACCCAAATGTATGGTTCGTTTACGCCCATGGAACAAATGC‑AAAATTTCGCGCCAGCCGCCAGGAAGATTTTACTTATCAGGAGAt                                                                                                      <  2:653183/149‑1 (MQ=255)                                               ctaTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGTCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGCTTTTCCTTATCAGGAGATACTTCt                                                                                                >  2:664726/3‑151 (MQ=255)                                                aaTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTg                                                                                               <  2:662175/151‑1 (MQ=255)                                                aaTACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTg                                                                                               <  2:815959/151‑1 (MQ=255)                                                            ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCttt                                                                                     <  1:773642/149‑1 (MQ=255)                                                            ttATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgc                                                                                   >  1:612318/1‑151 (MQ=255)                                                             tATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgc                                                                                   >  2:631042/1‑150 (MQ=255)                                                               tttGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgcatg                                                                                >  1:698961/1‑151 (MQ=255)                                                               tttGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgcat                                                                                 >  2:399818/1‑150 (MQ=255)                                                                 tGTCACTGTCGTTACTATATCGGCTGAAATTACTGAGGTCATACCCAAATGCATAGGTCGTTTACGCCCATTGAACAAATGC‑AAAATTTAGCGCCAGCCGCCACGAAGGTATTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgcatgc                                                                               >  2:362547/1‑150 (MQ=255)                                                                 tGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTtgcatgc                                                                               >  1:139862/1‑150 (MQ=255)                                                                     aCTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatg                                                                          >  2:197830/1‑151 (MQ=255)                                                                     aCTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatg                                                                          >  2:800171/1‑151 (MQ=255)                                                                        gTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatgca                                                                        >  2:92332/1‑150 (MQ=255)                                                                        gTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGcatgcacatgca                                                                        >  1:782522/1‑150 (MQ=255)                                                                                 atatCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAaagcaagc                                                                <  2:497565/149‑1 (MQ=255)                                                                                        cTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGgag                                                        <  2:398435/150‑1 (MQ=255)                                                                                            aaTTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACACGCAAAGCAAGCTGCTGGAGAACCg                                                   <  2:615983/151‑1 (MQ=255)                                                                                              ttAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGc                                                  >  2:532766/1‑150 (MQ=255)                                                                                                      gTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATg                                         >  1:665698/1‑151 (MQ=255)                                                                                                        cATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCt                                       <  1:33212/151‑1 (MQ=255)                                                                                                         aTACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCt                                       <  2:754472/150‑1 (MQ=255)                                                                                                          tACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTg                                      >  1:439051/1‑150 (MQ=255)                                                                                                               aaaTGGATAGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCt                                >  1:49783/1‑151 (MQ=255)                                                                                                                       aGTTCGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGAt                        <  1:69462/151‑1 (MQ=255)                                                                                                                           cGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTaa                     <  2:612169/150‑1 (MQ=255)                                                                                                                           cGTTTACGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTaa                     >  1:216018/1‑150 (MQ=255)                                                                                                                                aCGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGAc               >  1:12229/1‑151 (MQ=255)                                                                                                                                 cGCCCATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACg              <  1:126876/151‑1 (MQ=255)                                                                                                                                    ccATTGAACAAATGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACgttg           <  2:861507/151‑1 (MQ=255)                                                                                                                                             aaaTGC‑AAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCg  <  2:332576/151‑1 (MQ=255)                                                                                                                                                   |                                                                                                                                                  GAAAGTGATGGCGATTATATAACCCACAAGAATCATTTTTCTAAAACAATACATTTACTTTATTTGTCACTGTCGTTACTATATCGGCTGAAATTAATGAGGTCATACCCAAATGGATAGTTCGTTTACGCCCATTGAACAAATGCTAAAATTTCGCGCCAGCCGCCACGAAGATTTTCCTTATCAGGAGATCCTTCTGACTCGTCTTTGCATGCACATGCAAAGCAAGCTGCTGGAGAACCGCAATAAAATGCTGAAGGCTCAGGGGATTAACGAGACGTTGTTTATGGCG  >  NC_000913/2810658‑2810949

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑