Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091329,4960TA40.7% 68.3 / 30.9 54intergenic (+301/‑155)dapB/carAdihydrodipicolinate reductase/carbamoyl phosphate synthetase small subunit, glutamine amidotransferase
Reads supporting (aligned to +/- strand):  ref base T (26/6);  new base A (0/22);  total (26/28)
Fisher's exact test for biased strand distribution p-value = 5.02e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGA  >  NC_000913/29349‑29643
                                                                                                                                                    |                                                                                                                                                   
aaTGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaa                                                                                                                                                   >  2:40500/1‑150 (MQ=255)
   gTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATaaaaa                                                                                                                                                >  1:335169/1‑150 (MQ=255)
    tAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAAt                                                                                                                                              >  1:739814/1‑151 (MQ=255)
     aaaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGATGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAatat                                                                                                                                              >  2:466147/1‑150 (MQ=255)
      aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATcc                                                                                                                                            >  2:515682/1‑151 (MQ=255)
      aaTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATcc                                                                                                                                            <  1:760238/151‑1 (MQ=255)
       aTTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATccc                                                                                                                                           >  1:152046/1‑151 (MQ=255)
              ccATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCAtt                                                                                                                                     >  1:140543/1‑150 (MQ=255)
               cATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTa                                                                                                                                    >  1:89077/1‑150 (MQ=255)
                       ccttttttttCTGCTTGTTTTTATTTAATCCAATTTTTTTGCGGCAAAGGCTTTTCCAAGAAGAGTTTAGTGGATCTTTTTGCCGTTAAATGCCGGAAAAACATGAATGACCCAAAAAATAAAAAAAAAAATCCCGCCATTAAGTTGACt                                                                                                                            <  1:657912/146‑1 (MQ=255)
                           ttttttCTTCTCGTTTTTTTTTATTGCATTCTTTTTGCGGGAAAAGCTTTTCCAAAAACAGTTAAAATAATTTTTTTGTCTTTTAACCCCTAAAAAACATGCTTGAGCCAAAAAATAATATAAAAAACCCCGCCATTAAGTTGACttt                                                                                                                          <  2:436932/148‑1 (MQ=255)
                           ttttttCTGCTCTTTTTTTTTTCATGCATTCTTCTTGCTGCGCAAGTGTTTCCAAAAACAGGTAAGATAATTTTTTTGTAATTTAATGCTTGTAAAACATGCATGAGCAAAAAAATAATAAAAAAATCCCCGCCATTAAGTTGACTTTTa                                                                                                                        <  2:232455/150‑1 (MQ=255)
                             ttttCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAgcg                                                                                                                     >  2:161666/1‑151 (MQ=255)
                              tttCTGCTTTTTTTTATTTCATGAATTTTTTGTGCTGCACAACCTTTTTCCAGAACAGGTTAGATAATTTTTTTATCGTTTATTCCGTATAAACCATGCATGAGCCAAAAAATAAAATAAAAAACCCCGCCATTAAGTTGACTTTTAgcg                                                                                                                     <  2:508269/150‑1 (MQ=255)
                                    cTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCatat                                                                                                              >  1:742647/1‑151 (MQ=255)
                                     tctttttttttTCATGCATTTTTCTTGCTGCGCAAGCTTTTTCCAGAACAGGTTAAATGATTTTTTTGTCGCTTAATGCCTGAAAAACATGCATGAGCCAAAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCata                                                                                                               <  1:661990/146‑1 (MQ=255)
                                        ttttttttTCATGAATTCTTCTTGCTCCGAAAGCGTTTCCCAGAACAGTTTAGATGATTTTTTTGTCTCTTAATGCCTATAAAACATGCATGGCCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATAtctc                                                                                                           <  2:428617/150‑1 (MQ=255)
                                         ttttttttCATGCATTCTTCTTGCTGCGCAAGCGTTTCCCAAAACAGTTTAGATGATTTTTTTGTCGTTTAATGCCTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTcc                                                                                                          <  1:1952/150‑1 (MQ=255)
                                            tATTTCATGCATTCTTCTTGCTGCACAAGCTTTTTCAAAAACAGGTTAGATGATTTTTTTGCCGTTAAATGCCTGAAAAACATGCAGGAGCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGa                                                                                                       <  1:235732/150‑1 (MQ=255)
                                                   tGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgcc                                                                                               >  1:453097/1‑151 (MQ=255)
                                                   tGCAATATTCTTGTTGCGCAAGCGGTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTACTGCCTGTAAAACATGCATGAGCCACAAACTAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATgccgc                                                                                                >  2:803943/1‑150 (MQ=255)
                                                    gCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGccgccg                                                                                              >  1:343587/1‑151 (MQ=255)
                                                      aaTCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGtt                                                                                            >  1:714427/1‑151 (MQ=255)
                                                        ttttcttGCTGCGCAAGCGTTTTCAAAAACAGTTTAGATGTTTTTTTTGTCTTTAAATCCTTGAAAAACATGCATGAGCAAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGttt                                                                                           <  1:533618/148‑1 (MQ=255)
                                                                    cacagtgTTTTCCAAAAACAGGTAAAATATTTTTTTTTTCGTTTAATGCCTAAAAAACTTGAATGACCCAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtc                                                                                <  2:507030/144‑1 (MQ=255)
                                                                      aaGCTTTTTCCAGAACGGGTTAGATGATCTTTTTGTCGCTTAATGCCTGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATtcgt                                                                              <  1:800303/149‑1 (MQ=255)
                                                                        atcttttcaaacagcAGGTTAGATCGTCTTTTTCACTCTTCTCGCCTTAAAAACATGAGGGTGCCAAAAAATAATAAAAAAACCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGaaa                                                                                   <  2:379568/129‑1 (MQ=255)
                                                                           ttttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTa                                                                         >  1:309591/1‑149 (MQ=255)
                                                                            tttCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                      >  2:427696/1‑151 (MQ=255)
                                                                               ccAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGc                                                                      >  2:150340/1‑148 (MQ=255)
                                                                                  aaaaCAGTTTAGAATTTTTTTTTGTCTCTTAATCCCTGAAAAACTTGCATGCCCCACAAAATAATATAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGa                                                                   <  2:608728/147‑1 (MQ=255)
                                                                                   gAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGAttt                                                                >  2:288670/1‑151 (MQ=255)
                                                                                      cAGTTTAGATGATTTTTTTGTCGTTTAATGCCTAAAAAACAGGCATGACCCACAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGc                                                              <  1:289643/150‑1 (MQ=255)
                                                                                       aGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCAt                                                            >  1:129402/1‑151 (MQ=255)
                                                                                              atgttttttttGCCGTTTAATGCCTGAAAAACATGAATGACCCAAAAAATAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGa                                                         <  1:398988/141‑1 (MQ=255)
                                                                                                  tttttttGTCGTTTAATGCCTGAAAAACATGCATGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACg                                                   <  1:164762/147‑1 (MQ=255)
                                                                                                  tttttttGTCGCTTAATGCCTGAAAAACATGCATGACCCAAAAAAAAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                  <  1:301540/148‑1 (MQ=255)
                                                                                                   ttttttGTCGCTAAATGCCTGAAAAACATGCAGGAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGt                                                  <  1:647028/148‑1 (MQ=255)
                                                                                                           ctataattGCCTAAAAAACATGCATAAGCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCAtt                                           <  2:49121/141‑1 (MQ=255)
                                                                                                           cGCTTCATGCCTGTAAAACATGCATGAGCCACAAAATCATATAAAAAATCCCGCCATTAAGTTGTCTTTTAACGCCCATATCTCCAGAATGCCGCCTCTTGCCAGAAATTCGTCGGAAAGCAGATTTGCATTGATATACGTCATCAtt                                           >  2:572972/1‑148 (MQ=255)
                                                                                                               tAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAAtt                                    >  1:431728/1‑151 (MQ=255)
                                                                                                                aaTGCCTGTAAATCATGCATATGCCAAAAAAAAATAAAAAAATTCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTa                                   <  2:438908/151‑1 (MQ=255)
                                                                                                                                 catgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtg                   >  2:332677/1‑150 (MQ=255)
                                                                                                                                  atgAGCAAAAAAAAAATAAAAAAAACCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgag                 <  1:80448/151‑1 (MQ=255)
                                                                                                                                  aggggCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATaaa                      <  1:404540/142‑1 (MQ=255)
                                                                                                                                   tgAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGAATTGATTTACGTAATCATTGTGAATTAATATGCAAATAAagtgag                 >  1:526136/1‑150 (MQ=255)
                                                                                                                                   tgAACCAAAAAAAAATATAAAAATCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCaaa                          <  2:613971/141‑1 (MQ=255)
                                                                                                                                   tgAACCAAAAAAAAAAAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAag                     <  2:10800/146‑1 (MQ=255)
                                                                                                                                    gAGCCAAAAAATAATAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg               <  1:313673/151‑1 (MQ=255)
                                                                                                                                     aGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAagtgagtg               >  1:694615/1‑150 (MQ=255)
                                                                                                                                       ccACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat            >  1:255074/1‑151 (MQ=255)
                                                                                                                                          caAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATAtt         >  1:192248/1‑151 (MQ=255)
                                                                                                                                           aaaaaaaaaaaaaaaaCCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAat            <  2:660041/147‑1 (MQ=255)
                                                                                                                                            aaaaaaTAAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATtct       <  1:481592/151‑1 (MQ=255)
                                                                                                                                                aataAAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTgg   <  1:515807/151‑1 (MQ=255)
                                                                                                                                                 ataaAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGa  <  1:812476/151‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
AATGTAAATTTTGACCATTTGGTCCACTTTTTTCTGCTCGTTTTTATTTCATGCAATCTTCTTGCTGCGCAAGCGTTTTCCAGAACAGGTTAGATGATCTTTTTGTCGCTTAATGCCTGTAAAACATGCATGAGCCACAAAATAATATAAAAAATCCCGCCATTAAGTTGACTTTTAGCGCCCATATCTCCAGAATGCCGCCGTTTGCCAGAAATTCGTCGGTAAGCAGATTTGCATTGATTTACGTCATCATTGTGAATTAATATGCAAATAAAGTGAGTGAATATTCTCTGGA  >  NC_000913/29349‑29643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: