Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	298,953	0	A	C	47.5%	48.9 / 15.6	41	V661G (GTG→GGG)	paoC	PaoABC aldehyde oxidoreductase, Moco‑containing subunit
Reads supporting (aligned to +/- strand):  ref base A (20/1);  new base C (0/19);  total (20/21)
Fisher's exact test for biased strand distribution p-value = 3.05e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TGGCGTTATACACCGCGTTGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGG‑CCTTCA‑TCGGGGAGGATATGGGGTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGC  >  NC_000913/298807‑299101                                                                                                                                                       |                                                                                                                                                     tGGCGTTATACACCGCGTTGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGG‑CCTTCA‑TCGGGGAGGATATGGGGTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTc                                                                                                                                                  >  1:213054/1‑151 (MQ=255)       tataCACCGCGTTGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGG‑CCTTCA‑TCGGGGAGGATATGGGGTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATAccc                                                                                                                                            >  2:644582/1‑151 (MQ=255)        ataCACCGCGTTGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGG‑CCTTCA‑TCGGGGAGGATATGGGGTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATAcccc                                                                                                                                           >  1:769779/1‑151 (MQ=255)              cgcgTTGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGG‑CCTTCA‑TCGGGGAGGATATGGGGTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCat                                                                                                                                      >  1:269388/1‑150 (MQ=255)               gcgTTGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGG‑CCTTCA‑TCGGGGAGGATATGGGGTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCatat                                                                                                                                    >  2:803948/1‑151 (MQ=255)                  tgggCGATACCCGCGCTCACGCCGCACAGGCCCAGCTCACCAACCCCTTTGG‑CCTTCA‑TCGGGGAGGATATGGGGTCGGTATCACCC‑AGGAAAATCC‑CCTCCTGTTTGGGGATATCCGCATGACCCGGCCCCTCATACCCCGCCata                                                                                                                                     <  2:617166/145‑1 (MQ=255)                   tGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGG‑CCTTCA‑TCGGGGAGGATATGGGGTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTg                                                                                                                                >  1:10540/1‑151 (MQ=255)                      cGATAGCCGCGCTCACGCCGCACAGGCCCAGCCCCCCGACACCTTTGG‑CCTTCA‑TCGGGGAGGATAGGGGGTCGGTATCACCC‑AGGAAAATCC‑CCCCCTGTTTGGGGATACCCGCATGAACCGGCCCCTCATCCCCCGCCATATCGTGAtt                                                                                                                             <  1:512855/151‑1 (MQ=255)                              gcgcTCACCCCGCACAGGCCCATCTCCCCGACCCCTTGGG‑CCTTCA‑TCGGGGAGAATAGGGGGTCGGTATCATCC‑GGAAAAATCC‑CCTCCTGTTTTGGGATAACCGCAGGAACCGCCGCCTCATCCCCCGCCATATCGTGATTAACGa                                                                                                                        <  1:758587/148‑1 (MQ=255)                               cgcTCCCGCCGCACAGGCCCAGCCCCCCGACACCTTGGC‑CCTTCA‑TGGGGGAGGATATGGGGTCGGTACCACCC‑AGAAAAACCC‑CCTCCTTTTTGGGAATACCCAAAGGACCGGCCCCCTCATCCCCCGCCATATCGTGATTAACGaa                                                                                                                       <  2:189975/148‑1 (MQ=255)                                   cccgccgcACAGGCCCAGCTCACCGCCCCCTTTGG‑CCTTCA‑TGGGGGAGGATATGGGGTCGGTATCACCC‑AGAAAAATCC‑CCTCCTTTTTTGGAATATCCGCATGACCCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAg                                                                                                                   <  1:392074/146‑1 (MQ=255)                                   cACGCCGCACAGGCCCAGCTCACCGACACCTTTGG‑CCTTCA‑TCGGGGAGGATATGGGGTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGccc                                                                                                                >  2:806588/1‑151 (MQ=255)                                     cgccgcACAGCCCCAGCTCCCCGACCCCTTTGG‑CCTTCA‑TGGGGGAGGATATGGGGTCGGTATCACCC‑AGAAAAATCC‑CCTCCTGTTTGGGGATACCCGCATGAACCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGcc                                                                                                                 <  1:331567/148‑1 (MQ=255)                                      gccgcACAGGCCCAGCTCACCGACACCTTTGG‑CCTTCA‑TCGGGGAGGATATGGGGTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCaa                                                                                                              >  1:550625/1‑150 (MQ=255)                                           acaGGCCCAGCTCACCGACCCCTTTGC‑CCTTCA‑TCGGGGAGGATATGGGGTCGGTATCACCC‑AGAAAAACCC‑CCTCCTGTTTTGGGATATCCGCATGACCCGGCCCCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGt                                                                                                         <  1:307456/150‑1 (MQ=255)                                             agccccAGCTCACCGACCCCTTTGG‑CCTTCA‑TCGGGGAGGATAGGGGGTCGGTATCATCC‑AGGAAAATCC‑CCTCCTGTTTGGGGATACCCGCATGACCCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCa                                                                                                       <  1:271572/147‑1 (MQ=255)                                                     cccACCGAC‑CCTGTTG‑GCTTCACTCGGGGAGGATA‑GGGGGCGGTAACCGCCAAGAAAAATCA‑CCTCGTGTTTGGGAATATCGCCAGGACCCGGCCCCCCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGcc                                                                                              <  2:815208/149‑1 (MQ=255)                                                     cTCACCGACACCTTTGG‑CCTTCA‑TCGGGGAGGATATGGGGTCGGCATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGcc                                                                                              >  2:105644/1‑151 (MQ=255)                                                         ccgccccttttGC‑CTTTCA‑GGGGGGAGAATTTGGGGTCGTTATCACCA‑AGAAAAACCC‑CCTCCTTTTTGGGGATACCCCCATAACCCGCCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGc                                                                                           <  1:735897/142‑1 (MQ=255)                                                             ccccTTTGGCCCTTCG‑TCGGGGAGGATATGGGGTCGGTATCACCC‑AGAAAACCCC‑CCTCCTGTTTTGGAATACCCGAATAAACCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCGCCGCCAg                                                                                            <  2:662977/144‑1 (MQ=255)                                                                       ccTTCA‑TCGGGGAGGATATGGGGTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGc                                                                             >  1:699350/1‑151 (MQ=255)                                                                        cTTCA‑TCGGGGAGGATATGGGGTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGcc                                                                            >  1:176444/1‑151 (MQ=255)                                                                           cA‑TCGGGGAGGATATGGGGTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCcgcg                                                                         >  1:338639/1‑151 (MQ=255)                                                                            a‑TCGGGGAGGATAGGGGGCCGGTCACACCC‑AAAAAAACCC‑CCTCCTTTTTTGGATTACCCCAATAACCCGCCCCCTCACCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGcc                                                                            <  2:60017/147‑1 (MQ=255)                                                                                   gaggaTATGGGGTCGGAATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATAGACGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAAAGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTGCATCAGTGCCGCgcccatg                                                                   >  2:271587/1‑150 (MQ=255)                                                                                           ggtgcctgTATCATCC‑AGTAAACCCC‑CCCCTTGTGGGGGGATAGCCGAGTAGCCCGCCCCCGCATGCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCgcccatgcccat                                                              <  2:834400/140‑1 (MQ=255)                                                                                           ggggTGGGTATCACCC‑AGAAAAACCC‑CCTCCTTTTTTGGGATACCCGCATGACCCGCCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTc                                                          <  1:792071/151‑1 (MQ=255)                                                                                           ggggTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTc                                                          >  2:578840/1‑151 (MQ=255)                                                                                           ggggTCGGTATCACCA‑AGAAAAATCC‑CCCCCTGTTTGGGGATACCCGCATGACCCGCCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATa                                                             <  1:447727/148‑1 (MQ=255)                                                                                                 ttgttatccccgagAAAATCACCCTATTTTTTAGGGAAATCCCCCCGACCCGGCCCTCCAAACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCAtt                                                       <  1:434675/137‑1 (MQ=255)                                                                                                  gTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTgcgc                                                   >  2:6360/1‑151 (MQ=255)                                                                                                        tcC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGCAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGcc                                                  >  2:256578/1‑146 (MQ=255)                                                                                                        ccc‑AGAAAAATCC‑CCTCTTTTTTTGGGATATCCGCATAACCCGCCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCa                                                 <  2:48534/146‑1 (MQ=255)                                                                                                            agaaaaaCCC‑CCCCCTGTTTGGGGATACCCGAATGACCCGGCACCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGcc                                                  <  1:308852/140‑1 (MQ=255)                                                                                                            aGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCt                                          >  2:60549/1‑151 (MQ=255)                                                                                                                     cccctcctGTTTGGGGATCCCCGCATGACCCGCCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTg                                     <  1:90722/146‑1 (MQ=255)                                                                                                                     ccccccctTTTTGGGGATACCCGAATGACCCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTgc                                    <  1:680581/144‑1 (MQ=255)                                                                                                                              tttgGGGATCTCCGAATGACCCGGCCCCTCATCCCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGtt                            <  1:671881/148‑1 (MQ=255)                                                                                                                               tttGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCg                        >  1:362864/1‑151 (MQ=255)                                                                                                                                                aaCCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAACCGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACATGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTcc        >  2:584183/1‑150 (MQ=255)                                                                                                                                                      cACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTgcagc  >  2:34575/1‑150 (MQ=255)                                                                                                                                                       |                                                                                                                                                     TGGCGTTATACACCGCGTTGGCGATAGCCGCGCTCACGCCGCACAGGCCCAGCTCACCGACACCTTTGG‑CCTTCA‑TCGGGGAGGATATGGGGTCGGTATCATCC‑AGGAAAATCA‑CCTCCTGTTTTGGGATATCCGCATGAACCGGCACCTCATACCCCGCCATATCGTGATTAACGAAGTAGCCCAAACGGTCATCCACCGCCAGCTCCTCCATCAGTGCCGCGCCCATGCCCATAGTCATTGCGCCAATGACCTGGCTGCGCGCAGTTTTCGGATTCAGGATGCGTCCTGCAGC  >  NC_000913/298807‑299101

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑