Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913941,7130TG42.9% 71.5 / 14.1 42V252G (GTG→GGG) dmsAdimethyl sulfoxide reductase, anaerobic, subunit A
Reads supporting (aligned to +/- strand):  ref base T (2/22);  new base G (18/0);  total (20/22)
Fisher's exact test for biased strand distribution p-value = 5.37e-10
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACG  >  NC_000913/941568‑941853
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tactCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCtt                                                                                                                                         >  1:670008/1‑151 (MQ=255)
             aaaTTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTATCTTTaa                                                                                                                              >  1:155834/1‑149 (MQ=255)
                   cGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACg                                                                                                                      <  1:103000/151‑1 (MQ=255)
                      aaGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCa                                                                                                                   <  1:229339/151‑1 (MQ=255)
                         gTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTATCTTGAAAAGGCACGcc                                                                                                                    >  2:414410/1‑147 (MQ=255)
                           ttGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGaaa                                                                                                               <  2:484907/150‑1 (MQ=255)
                           ttGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTAATATCTTTAAAAAGCAAGCCAGaaa                                                                                                               >  1:219466/1‑150 (MQ=255)
                            tGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGaaa                                                                                                               <  2:764771/149‑1 (MQ=255)
                            tGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTTAAAAAGCACGCCCGaaa                                                                                                               >  1:249636/1‑149 (MQ=255)
                             gAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCt                                                                                                            <  2:197527/151‑1 (MQ=255)
                                  ataCCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAAt                                                                                                         <  1:611958/149‑1 (MQ=255)
                                          ggcggcTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAAGCACGCCCGAAATCTAATGccc                                                                                                     >  1:786993/1‑145 (MQ=255)
                                                  ggCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatcatcatc                                                                                       <  1:627604/151‑1 (MQ=255)
                                                   gCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGATTTATAACTTGAACAGGCACGCCAGAAAAATAATGCCCCCATGatcctcattg                                                                                      >  2:741526/1‑149 (MQ=255)
                                                     aGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGAt                                                                                    <  1:751690/151‑1 (MQ=255)
                                                        tGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGAATTTCTATCTTTAAAAAGCACGCCAGAAATATAAAGCCCGCATGATCATTatccatcc                                                                                  >  2:270405/1‑150 (MQ=255)
                                                         ggCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCcg                                                                                 <  1:314040/150‑1 (MQ=255)
                                                                 cccGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATAcc                                                                        <  1:212568/151‑1 (MQ=255)
                                                                  ccGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATAcc                                                                        <  2:344779/150‑1 (MQ=255)
                                                                   cGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAAGCACGCCAGAAAACTAAAGCCCGCATGATCATCATCGATCCGCGCTATAcc                                                                        >  1:690271/1‑149 (MQ=255)
                                                                   cGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGACTTTCTATCTTGAACAGGCACGCCAGAAAACTAATGCCCGCATGATCATCATCCATCCGCGCta                                                                            >  1:826906/1‑145 (MQ=255)
                                                                        gATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCTTTAACAGGCACGCCCGAAATCTAATGCCCGCATGATCATTATCGAACCGCGCTATACCCACAcc                                                                  >  1:263124/1‑150 (MQ=255)
                                                                             cGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTAATATCTTTAACAAGCACGCCAGAAATCTAATGCCCGGCTGATCATCACCGATCCGCGGTATAACGACACCGGTGcc                                                            >  2:149082/1‑151 (MQ=255)
                                                                              gAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCg                                                           <  1:414511/151‑1 (MQ=255)
                                                                              gAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGGGCCg                                                           >  1:8401/1‑151 (MQ=255)
                                                                              gAAAACAGTAAGCTGGTAGTGCTGTTTGCTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCg                                                           <  2:85143/151‑1 (MQ=255)
                                                                                     gTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGa                                                    <  1:569378/151‑1 (MQ=255)
                                                                                         gCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGAt                                                <  2:379249/151‑1 (MQ=255)
                                                                                          cTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCAGAAAACTAATGCCCGCCTGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGGGCGAAg                                                  >  1:258934/1‑148 (MQ=255)
                                                                                            ggTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGa                                              <  2:337886/150‑1 (MQ=255)
                                                                                             gTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCCGAAATCTAATGCCCCCATGATCATCCTCGATCCGCGCTATACCGACACCGGGGCCGGGGGCGGAGATGAg                                             >  1:651077/1‑150 (MQ=255)
                                                                                                gTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGa                                         <  1:91471/151‑1 (MQ=255)
                                                                                                       ttGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGTGCTTACTATCTTGAACAAGAACGCCCGAAAACTAATGCCCGCATGATAATCATCCATCCGCGCTATACCGACACCCGTTCCGGGCGCGAAGATGAGTGGATccc                                     >  2:141652/1‑148 (MQ=255)
                                                                                                         ggTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTc                                <  2:361717/151‑1 (MQ=255)
                                                                                                               aaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCgg                          <  2:173575/151‑1 (MQ=255)
                                                                                                                aCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTTCTATCTTGAACAGGCACGCCAGAAAACTAATGCCCGCATGATCATCCTCGGTCCGCCCCATACCCACACCCGGGCCGGGCGCGAAGATGAGTGGGTCCCTATTCGTCCggg                         >  1:325018/1‑151 (MQ=255)
                                                                                                                  ccTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGaa                       <  1:340422/151‑1 (MQ=255)
                                                                                                                    tGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTa                                   <  1:174674/137‑1 (MQ=255)
                                                                                                                    tGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCCCGCTATACCGACACCCGTGCCGGGCGCGAAGATGGGTGGGTccccaagaacgggagag                       >  2:174635/1‑135 (MQ=255)
                                                                                                                        gAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATg                 <  1:11794/151‑1 (MQ=255)
                                                                                                                                      tGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAAc   >  1:269153/1‑151 (MQ=255)
                                                                                                                                       ggCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACg  >  1:403317/1‑151 (MQ=255)
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TACTCCTCCGCGCAAATTGCGGAAGGTTTGAACTATACCTACGGCGGCTGGGCAGATGGCAACAGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCGGGCGCGAAGATGAGTGGATCCCTATTCGTCCGGGAACAGATGCCGCACTGGTTAACG  >  NC_000913/941568‑941853

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑