Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913635,0920AT40.5% 49.1 / 13.3 37D95E (GAT→GAAybdMSpo0J family protein, ParB‑like nuclease domain
Reads supporting (aligned to +/- strand):  ref base A (8/14);  new base T (15/0);  total (23/14)
Fisher's exact test for biased strand distribution p-value = 7.00e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACGTTGTTTGGGTTGTAATCATTAGGCATAAGTTGGCTGTTTTTAACCCACAGAACGCAATCC  >  NC_000913/634948‑635236
                                                                                                                                                |                                                                                                                                                 
aCGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCCTGGCCTTTTTTTcc                                                                                                                                                >  2:277283/1‑148 (MQ=255)
               gATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTTTTCGTATGTGTGACcaca                                                                                                                                >  1:331646/1‑148 (MQ=255)
                     ttGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTTAAAACCAACCACGATTTTCCTGGCATTTTTTTCCGTATGTGTGACCACAAaggg                                                                                                                           >  1:539697/1‑147 (MQ=255)
                       gATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAAtgggttgggt                                                                                                                     <  2:288557/151‑1 (MQ=255)
                          tACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTGTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTaa                                                                                                                   <  2:305234/150‑1 (MQ=255)
                                      ccAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGGATTTTTTTTCGTATGTGTGACCACAATGGGGTGGGTAAAAACATCTAttt                                                                                                       >  1:77215/1‑150 (MQ=255)
                                        aGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAAACATCCACGATTTTCATGGGATTTTTATCCGTATGTGTGACCACAATGGGGTGGGTAAAACCATCCAttt                                                                                                       >  2:272198/1‑148 (MQ=255)
                                            aGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTTTCCCCATGTGTGACCACAAAGGGGTGGGGAAAACCATCTTTTTCAATgg                                                                                                 >  2:376380/1‑150 (MQ=255)
                                                cACCGGCAAATAACCTTTTGAGCGTAATTTTAATGATGATGAGCCTTTAACAATTTCATGGCGGTGAAAACCAGCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGAttt                                                                                             <  1:542369/150‑1 (MQ=255)
                                                  ccGGCAAATAACCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTg                                                                                          <  1:445612/151‑1 (MQ=255)
                                                     gCAAATAACCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAAACATTCACGATTTTCATGGGATTTTTTTTCGGTTGGGTGACCCAAAAGGGGTTGGGAAAACCATCTATTTTAAAGGGttttt                                                                                           >  1:249837/1‑147 (MQ=255)
                                                        aaTAACCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGcagcag                                                                                    <  1:440983/151‑1 (MQ=255)
                                                           aaCCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGttt                                                                                 <  1:364315/151‑1 (MQ=255)
                                                           aaCCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGttt                                                                                 <  1:16482/151‑1 (MQ=255)
                                                           aaCCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGCGAAAAAAATCCACGATTTTCATTGCATTTTTTTCCCTTTGTGTGACCACAAATGGTTGGGTAAAACCACCTTTTTCAATTGAATTTTTTAGGAGttt                                                                                 >  1:165393/1‑151 (MQ=255)
                                                            aCCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGGTTTTCAAGGCGTTTTTATTCACATTTGTTAGCACAAAAGGTTGGGTGAAACCATCTATTTTAATTGtttttttttccagattt                                                                                >  1:509043/1‑140 (MQ=255)
                                                                 ttAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAAACATCCACGATTTTCATGGCATTTTTATCCGTATGTGTGACCACAATGGGGTGGGTAAAACCATCTATTTCAATGGATTTTTTCAGCAGtttttttt                                                                            >  2:384301/1‑150 (MQ=255)
                                                                 ttAAGCGTAACTTTAATGATGATGAGCCGGTACCAATTTCATAGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCTCAATGGGTTGGGTAAAGCCATCTATTTCAATGTATTGTTGCATCAGttttttt                                                                             <  1:124623/149‑1 (MQ=255)
                                                                             ttAATGATGATGAGCCTTTACCAATTTCATGGCGGTGGAAACCAACCACGATTTCCCTGGCATTTTTTTCCTTATGTGGGACCACAATGGGGTGGGGAAAACCATCTATTTCAATGGATTTTTTTAGCAGTTTTTTTTTGGGGGGTGcccc                                                               >  1:128532/1‑149 (MQ=255)
                                                                             ttAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAAACATCCACCGTTTCCATGGGATTTTTTTCCGCATGTGTGACCAAAATGGGGTGGGGAAAACCCTCTATTTTAATGGATTTTTGTAGCAGTTTTTTTTCgggggggggc                                                                 >  2:390581/1‑145 (MQ=255)
                                                                              tAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTTCATGGCATTTTTTTCCGTATGTGTGACCACAATGGGTTGGGTAAAAACATCTATTTCAATGGATTTTTTCAGCAGTTTTTTTTTGggtgggggccc                                                               >  1:575891/1‑144 (MQ=255)
                                                                              tAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAAACATCCACGAATTACCTTGTATTTTTTTCCCTATGTTTGACCCCCAATGGTTGGGTAAAAACATCTATTTTAATGGATTTTTGCAGTAAGCTTTTTTCggg                                                                        >  1:401867/1‑141 (MQ=255)
                                                                                atgatgatgAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACgtt                                                            <  2:307357/151‑1 (MQ=255)
                                                                                 tgatgatgaGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTTTCCGTATGTGTGACCCCAATGGGTTGGGTAAAAACATCTATTTCTATGGATTTTTGCAGCAGTTTTTTTTTGGGTGGTGCCACgttg                                                           >  2:366643/1‑151 (MQ=255)
                                                                                  gatgatgaGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACgttgt                                                          <  2:249878/151‑1 (MQ=255)
                                                                                  gatgatgaGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACgttg                                                           <  1:125351/150‑1 (MQ=255)
                                                                                   atgatgaGCCTTTACCAATTTCATGGCGGTGAAAAACATCCACGATTTTCCTTGCATTTTTTTTCGTATGGGTGACCACAATGGGTTGGGTAAAACCATCTTTTTCAATGGGTTTTTTCCGCAGTTTTTTTTCTGGTGGGGCCACgttgt                                                          >  2:512533/1‑150 (MQ=255)
                                                                                               tACCAATTTCATGGCGGTGAAAAACAACCACCAGTTCCATGGCATTTTTTTCCCTATGTGTGGCCACAATGGGGTGGGTAAAACCATCTTTTTCCATGGATTTTTTCAGCAGTTTTTTTTTGGGGGGTGCCACCTTGTTTTGGTTGTTAt                                              >  1:498021/1‑150 (MQ=255)
                                                                                                       tCATGGCGGTGAAAACCATCCACGAATTTCATGGGCTTTTTTTCCGTATGGGTGAACACAATGGGTTGGGGACAACCATCTATTTCTATGGGTTTTTTCAGCAGTTTTTTTTTGGGGGGTGCCACGTTGTTTGGGGTGGAATCCTTTgg                                       >  1:232284/1‑149 (MQ=255)
                                                                                                         aTGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGGATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTTGGGGGGGGCCACGTTGTTTGGGTTGTAATCATTAGGCAt                                    >  1:182461/1‑150 (MQ=255)
                                                                                                          tGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACGTTGTTTGGGTTGTAATCATTAGGCATa                                   >  2:346753/1‑150 (MQ=255)
                                                                                                                 gAAGACCATCCGCGATTTGCATGGCATTTTTATCCGTATGTGTGACGACAATGGGTTGGGTAAAACCATCTACTTCAATGGATTTTTGCAGAAGTTATATTGCGGGTGGAGCCGCGTTGGTTGGGGTGTAGTCAGTAGGCATAAGttag                             <  1:25533/149‑3 (MQ=255)
                                                                                                                   aaaCCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACGTTGTTTGGGTTGTAATCATTAGGCATAAGTTGGCTGt                         <  2:533955/151‑1 (MQ=255)
                                                                                                                       cATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACGTTGTTTGGGTTGTAATCATTAGGCATAAGTTGGCTGttttt                     <  2:357699/151‑1 (MQ=255)
                                                                                                                              gATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACGTTGTTTGGGTTGTAATCATTAGGCATAAGTTGGCTGTTTTTAACCcac              >  1:9776/1‑151 (MQ=255)
                                                                                                                              gATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACGTTGTTTGGGTTGTAATCATTAGGCATAAGTTGGCTGTTTTTAACCcac              >  1:2656/1‑151 (MQ=255)
                                                                                                                                          aTTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACGTTGTTTGGGTTGTAATCATTAGGCATAAGTTGGCTGTTTTTAACCCACAGAACGCAATcc  >  2:265041/1‑151 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
ACGAATAGTCGCGGCGATGCGTTGATTACGCGTACCTTCCAGACAGGTCACCGGCAAATAACCTTTTAAGCGTAATTTTAATGATGATGAGCCTTTACCAATTTCATGGCGGTGAAAACCATCCACGATTTCCATGGCATTTTTATCCGTATGTGTGACCACAATGGGTTGGGTAAAACCATCTATTTCAATGGATTTTTGCAGCAGTTTTTTTTCGGGTGGTGCCACGTTGTTTGGGTTGTAATCATTAGGCATAAGTTGGCTGTTTTTAACCCACAGAACGCAATCC  >  NC_000913/634948‑635236

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: